Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,589,933 G→A 100% T55I (ACC→ATC)  ypfJ ← putative neutral zinc metallopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,589,9330GA100.0% 648.6 / ‑6.7 196T55I (ACC→ATC) ypfJputative neutral zinc metallopeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (112/82);  minor base C (0/1);  total (112/84)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 196 total aligned reads displayed.

TACCCATCTTCTCGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCACTTTC  >  CP009273/2589790‑2590075
                                                                                                                                               |                                                                                                                                              
tACCCATCTTCTCGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTaa                                                                                                                                            <  2:2041257/148‑1 (MQ=255)
tACCCATCTTCTCGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTaa                                                                                                                                            <  1:2884074/148‑1 (MQ=255)
 aCCCATCTTCTCGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTaaa                                                                                                                                           >  2:1091624/1‑148 (MQ=255)
   ccATCTTCTCGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATc                                                                                                                                         >  2:718496/1‑148 (MQ=255)
     aTCTTCTCGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAAGTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGAGGAAATCaa                                                                                                                                       >  1:2722186/1‑148 (MQ=255)
       cttctCGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAaca                                                                                                                                     <  2:1351008/148‑1 (MQ=255)
       cttctCGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAaca                                                                                                                                     >  1:3056469/1‑148 (MQ=255)
          ctcGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCa                                                                                                                                  >  2:2849831/1‑148 (MQ=255)
          ctagAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCAGCTAGGTGAATTTTGCGGCTTAGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCa                                                                                                                                  <  2:599953/145‑1 (MQ=255)
             gAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCAtag                                                                                                                               <  1:1424668/148‑1 (MQ=255)
               aCTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCAtagta                                                                                                                             >  2:2253383/1‑148 (MQ=255)
               aCTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCAtagta                                                                                                                             >  1:2423536/1‑148 (MQ=255)
               aCTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCAtagta                                                                                                                             >  2:592495/1‑148 (MQ=255)
                cTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCAtagtag                                                                                                                            >  1:1149461/1‑148 (MQ=255)
                cTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCAtagtag                                                                                                                            >  1:331593/1‑148 (MQ=255)
                  gttgtCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGcc                                                                                                                          >  2:2841811/1‑148 (MQ=255)
                    tgtCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTg                                                                                                                        >  1:3116328/1‑148 (MQ=255)
                    tgtCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTg                                                                                                                        >  1:631676/1‑148 (MQ=255)
                       ccccAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCaa                                                                                                                     <  1:2184441/148‑1 (MQ=255)
                       ccccAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCaa                                                                                                                     <  1:117966/148‑1 (MQ=255)
                        cccAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAAc                                                                                                                    >  2:3158986/1‑148 (MQ=255)
                         ccAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAAcc                                                                                                                   <  2:2081351/148‑1 (MQ=255)
                          cAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCa                                                                                                                  >  1:286865/1‑148 (MQ=255)
                           aGGTGTCTTCCGTGGTTGCCAGACTCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAg                                                                                                                 <  2:1706495/148‑1 (MQ=255)
                           aGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAg                                                                                                                 >  2:86183/1‑148 (MQ=255)
                            ggTGTCTTCCGTGGTTGCCAGAATCACCGATGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGc                                                                                                                <  1:1751052/148‑1 (MQ=255)
                            ggTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGc                                                                                                                >  2:156356/1‑148 (MQ=255)
                            ggTGTCTTCCGTGGTTGACAGAATCACCGAGGTGAATTTTGCGTCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGc                                                                                                                <  1:229200/148‑1 (MQ=255)
                                   tCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTa                                                                                                         <  2:1331243/148‑1 (MQ=255)
                                   tCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTa                                                                                                         <  2:449889/148‑1 (MQ=255)
                                   tCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTa                                                                                                         >  2:2550692/1‑148 (MQ=255)
                                   tCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTa                                                                                                         >  2:2739389/1‑148 (MQ=255)
                                     cGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATc                                                                                                       >  1:2660405/1‑148 (MQ=255)
                                     cGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATc                                                                                                       >  1:1633641/1‑148 (MQ=255)
                                       tGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAg                                                                                                     >  1:306947/1‑148 (MQ=255)
                                           tGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTaaa                                                                                                 <  2:638682/148‑1 (MQ=255)
                                              cAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATc                                                                                              <  2:1841733/148‑1 (MQ=255)
                                               aGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCa                                                                                             <  2:63173/148‑1 (MQ=255)
                                                gAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAg                                                                                            >  2:1179513/1‑148 (MQ=255)
                                                gAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAg                                                                                            >  2:1652597/1‑148 (MQ=255)
                                                 aaTCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGc                                                                                           <  1:913348/148‑1 (MQ=255)
                                                 aaTCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGc                                                                                           >  2:674350/1‑148 (MQ=255)
                                                  aTCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGcc                                                                                          <  2:2119663/148‑1 (MQ=255)
                                                   tCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGccc                                                                                         >  2:2929659/1‑148 (MQ=255)
                                                    cACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCg                                                                                        >  2:2057825/1‑148 (MQ=255)
                                                      ccGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGcc                                                                                      >  1:179643/1‑148 (MQ=255)
                                                      ccGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGcc                                                                                      <  1:2778569/148‑1 (MQ=255)
                                                         aGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCttt                                                                                   >  2:2548189/1‑148 (MQ=255)
                                                          ggTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTa                                                                                  >  2:1666089/1‑148 (MQ=255)
                                                             gAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCg                                                                               <  1:452243/148‑1 (MQ=255)
                                                             gAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCg                                                                               <  1:1236775/148‑1 (MQ=255)
                                                              aaTTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGc                                                                              >  1:1380605/1‑148 (MQ=255)
                                                                ttttGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCtt                                                                            >  2:1811862/1‑148 (MQ=255)
                                                                ttttGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCtt                                                                            <  2:3129188/148‑1 (MQ=255)
                                                                ttttGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCtt                                                                            >  1:3143531/1‑148 (MQ=255)
                                                                         ttCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGa                                                                   >  1:599159/1‑148 (MQ=255)
                                                                          tCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGaa                                                                  >  2:1767372/1‑148 (MQ=255)
                                                                            gTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGaaaa                                                                <  1:1559999/148‑1 (MQ=255)
                                                                            gTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGaaaa                                                                <  2:1646330/148‑1 (MQ=255)
                                                                            gTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGaaaa                                                                <  1:3291629/148‑1 (MQ=255)
                                                                              cTTCATTTGGGCTTATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAACTCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAAcc                                                              <  1:757145/148‑1 (MQ=255)
                                                                              cTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAAcc                                                              >  2:279491/1‑148 (MQ=255)
                                                                              cTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAAcc                                                              >  1:2755522/1‑148 (MQ=255)
                                                                              cTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAAcc                                                              >  1:2251641/1‑148 (MQ=255)
                                                                              cTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAAcc                                                              <  2:1434731/148‑1 (MQ=255)
                                                                               ttCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCg                                                             <  1:1234699/148‑1 (MQ=255)
                                                                                  aTTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGa                                                          <  2:1126255/148‑1 (MQ=255)
                                                                                    ttGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGAcc                                                        >  2:3180022/1‑148 (MQ=255)
                                                                                    ttGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGAcc                                                        >  2:3102864/1‑148 (MQ=255)
                                                                                       ggCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGcc                                                     <  1:2035165/148‑1 (MQ=255)
                                                                                           aaTTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATa                                                 >  2:737766/1‑148 (MQ=255)
                                                                                             ttGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGa                                               >  1:161708/1‑148 (MQ=255)
                                                                                                aCCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATgg                                            <  2:3056469/148‑1 (MQ=255)
                                                                                                aCCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATgg                                            >  1:2792159/1‑148 (MQ=255)
                                                                                                  cgcgTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTTAATCAACACCATAGTAGCCTGCAACCAGCACGACTATGAGTAAAATCTGCCCGGCTTTACCTCTTGGCAGGCGAAAACCGGGAGCGCCGTTAGATGGac                                          <  2:3210896/148‑1 (MQ=255)
                                                                                                    cgTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGacca                                        >  1:1342653/1‑148 (MQ=255)
                                                                                                    cgTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGacca                                        >  1:3312782/1‑148 (MQ=255)
                                                                                                      ttGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGaccacc                                      >  2:914137/1‑148 (MQ=255)
                                                                                                      ttGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGaccacc                                      >  1:1091835/1‑148 (MQ=255)
                                                                                                        gATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCag                                    <  2:1149461/148‑1 (MQ=255)
                                                                                                         aTTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCaga                                   >  1:2682573/1‑148 (MQ=255)
                                                                                                          ttgttgttgGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCagag                                  >  1:509258/1‑148 (MQ=255)
                                                                                                           tgttgttgGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGc                                 >  1:2707727/1‑148 (MQ=255)
                                                                                                             ttgttgGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTg                               >  2:2315656/1‑148 (MQ=255)
                                                                                                              tgttgGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGt                              >  2:1267355/1‑148 (MQ=255)
                                                                                                                   ggAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTgcgc                         >  1:1071232/1‑148 (MQ=255)
                                                                                                                        ccGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTc                    <  1:977103/148‑1 (MQ=255)
                                                                                                                        ccGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTc                    >  2:372779/1‑148 (MQ=255)
                                                                                                                        ccGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTc                    >  2:3110407/1‑148 (MQ=255)
                                                                                                                        ccGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTc                    >  2:1541483/1‑148 (MQ=255)
                                                                                                                        ccGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTc                    <  1:1652592/148‑1 (MQ=255)
                                                                                                                          ggCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCtt                  <  1:592495/148‑1 (MQ=255)
                                                                                                                          ggCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCtt                  <  1:1653691/148‑1 (MQ=255)
                                                                                                                            cTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCa                >  1:1127025/1‑148 (MQ=255)
                                                                                                                               cccGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCTGAGCTGTTGCGCCTGTCGTCACCa             >  2:2199672/1‑148 (MQ=255)
                                                                                                                                ccGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACAt            <  1:37126/148‑1 (MQ=255)
                                                                                                                                ccGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACAt            <  1:2944906/148‑1 (MQ=255)
                                                                                                                                ccGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACAt            <  1:2430707/148‑1 (MQ=255)
                                                                                                                                 cGGTCCTCAACCCGCTTAAAGCACCACCAGAGGAGCCTGCAACCACCACGACTATCAGTAAAATCAGCCCGCCTGTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACAtt           <  2:2722186/148‑1 (MQ=255)
                                                                                                                                 cGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACAtt           <  2:513007/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                              
TACCCATCTTCTCGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCACTTTC  >  CP009273/2589790‑2590075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑