Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,606,516 G→T 100% D420Y (GAC→TAC)  hyfR → hydrogenase‑4 transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,606,5160GT100.0% 708.9 / NA 215D420Y (GAC→TAC) hyfRhydrogenase‑4 transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (99/116);  total (99/116)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 215 total aligned reads displayed.

TTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGA  >  CP009273/2606372‑2606660
                                                                                                                                                |                                                                                                                                                
ttGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACa                                                                                                                                               >  1:822433/1‑148 (MQ=255)
 tGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACaa                                                                                                                                              <  1:848731/148‑1 (MQ=255)
 tGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACaa                                                                                                                                              >  2:1565836/1‑148 (MQ=255)
    tttGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAgg                                                                                                                                           >  1:2568933/1‑148 (MQ=255)
     ttGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAggg                                                                                                                                          >  2:3218792/1‑148 (MQ=255)
      tgtgGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCTCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAgggg                                                                                                                                         <  1:2740545/148‑1 (MQ=255)
      tgtgGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAgggg                                                                                                                                         <  1:1000333/148‑1 (MQ=255)
      tgtgGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAgggg                                                                                                                                         <  2:2261088/148‑1 (MQ=255)
        tggtgAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCg                                                                                                                                       <  1:1440008/148‑1 (MQ=255)
        tggtgAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCg                                                                                                                                       <  1:1322942/148‑1 (MQ=255)
         ggtgAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGt                                                                                                                                      <  2:746625/148‑1 (MQ=255)
            gAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTa                                                                                                                                   <  1:419383/148‑1 (MQ=255)
              aaCCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACt                                                                                                                                 <  2:1820129/148‑1 (MQ=255)
               aCCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTg                                                                                                                                <  1:184602/148‑1 (MQ=255)
                ccGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTgg                                                                                                                               <  2:2905653/148‑1 (MQ=255)
                ccGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTgg                                                                                                                               >  1:2930145/1‑148 (MQ=255)
                 cGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGt                                                                                                                              <  2:426770/148‑1 (MQ=255)
                     aCTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGa                                                                                                                          >  2:1159422/1‑148 (MQ=255)
                     aCTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGa                                                                                                                          >  2:1484462/1‑148 (MQ=255)
                       tGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGAtt                                                                                                                        >  1:2157973/1‑148 (MQ=255)
                       tGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGAtt                                                                                                                        >  1:2839070/1‑148 (MQ=255)
                          cAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAAt                                                                                                                     <  1:308111/148‑1 (MQ=255)
                            aaGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATAc                                                                                                                   <  2:1233683/148‑1 (MQ=255)
                            aaGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATAc                                                                                                                   >  1:1642939/1‑148 (MQ=255)
                              gagGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATAccc                                                                                                                 >  2:1365426/1‑148 (MQ=255)
                                 gTGATCGCCAGAGCTATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATc                                                                                                              <  1:1565836/148‑1 (MQ=255)
                                 gTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATc                                                                                                              >  2:515990/1‑148 (MQ=255)
                                   gATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGt                                                                                                            >  1:2201724/1‑148 (MQ=255)
                                     tCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTgg                                                                                                          <  2:3047157/148‑1 (MQ=255)
                                     tCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTgg                                                                                                          <  2:3047134/148‑1 (MQ=255)
                                     tCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCCACAAGCCGCTGGTCAAAATAAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTgg                                                                                                          <  1:275780/148‑1 (MQ=255)
                                      cGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCGTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGt                                                                                                         >  1:3015057/1‑148 (MQ=255)
                                        ccAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCg                                                                                                       <  1:3203236/148‑1 (MQ=255)
                                        ccAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCg                                                                                                       <  2:2272190/148‑1 (MQ=255)
                                         cAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCNGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGt                                                                                                      >  1:1681831/1‑148 (MQ=255)
                                           gagCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGttt                                                                                                    >  1:1257204/1‑148 (MQ=255)
                                           gagCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGtgt                                                                                                    >  2:1978437/1‑146 (MQ=255)
                                              cGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGa                                                                                                 >  2:2474989/1‑148 (MQ=255)
                                               gATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGaa                                                                                                <  1:3148614/148‑1 (MQ=255)
                                                  ccATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAAtt                                                                                             >  1:3268413/1‑148 (MQ=255)
                                                   cATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTg                                                                                            <  2:2023205/148‑1 (MQ=255)
                                                   cATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTg                                                                                            >  2:62576/1‑148 (MQ=255)
                                                   cATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTg                                                                                            >  2:1054461/1‑148 (MQ=255)
                                                   cATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTg                                                                                            <  1:2997238/148‑1 (MQ=255)
                                                    aTCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGc                                                                                           <  1:1906667/148‑1 (MQ=255)
                                                      cAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCg                                                                                         >  2:3125488/1‑148 (MQ=255)
                                                        aCTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGAt                                                                                       >  1:173462/1‑148 (MQ=255)
                                                         cTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATg                                                                                      <  1:252717/148‑1 (MQ=255)
                                                          ttAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATgg                                                                                     >  1:1183213/1‑148 (MQ=255)
                                                             gCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATggcgg                                                                                  >  1:2123100/1‑148 (MQ=255)
                                                                  cgacgcgacAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGTAATTTCCGATGGCGGCCCgt                                                                             >  1:683592/1‑148 (MQ=255)
                                                                  cgacgcgacAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTGTTGTAATTTCCGATGGCGGCACgt                                                                             >  1:322000/1‑148 (MQ=255)
                                                                           aaGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCg                                                                    <  2:1703946/148‑1 (MQ=255)
                                                                             gCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGTAATTGCCGATGGCGGCACGTTGTTTCTCGAt                                                                  >  1:394631/1‑148 (MQ=255)
                                                                             gCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGAt                                                                  >  1:2669469/1‑148 (MQ=255)
                                                                              ccGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATg                                                                 <  1:611689/148‑1 (MQ=255)
                                                                              ccGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATg                                                                 >  2:374249/1‑148 (MQ=255)
                                                                                gCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGaa                                                               <  2:1157209/148‑1 (MQ=255)
                                                                                 cTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTTAAAATTCCGATGGCCGCACGTTTTTTCTCGATGaaa                                                              >  1:1389825/1‑148 (MQ=255)
                                                                                 cTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGaaa                                                              >  2:326808/1‑148 (MQ=255)
                                                                                 cTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGaaa                                                              <  2:926017/148‑1 (MQ=255)
                                                                                 cTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGaaa                                                              <  2:111945/148‑1 (MQ=255)
                                                                                  tGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAAt                                                             <  1:2003561/148‑1 (MQ=255)
                                                                                    gTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTg                                                           >  1:1394769/1‑148 (MQ=255)
                                                                                    gTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTg                                                           <  1:1883990/148‑1 (MQ=255)
                                                                                         aaTCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGAt                                                      >  2:1300684/1‑148 (MQ=255)
                                                                                         aaTCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGAt                                                      <  2:2346329/148‑1 (MQ=255)
                                                                                          aTCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATc                                                     <  2:164278/148‑1 (MQ=255)
                                                                                             aaCTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGc                                                  <  2:2930145/148‑1 (MQ=255)
                                                                                              aCTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGcc                                                 >  1:1082626/1‑148 (MQ=255)
                                                                                                  cgcTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTa                                             <  1:445369/148‑1 (MQ=255)
                                                                                                  cgcTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTa                                             <  1:673816/148‑1 (MQ=255)
                                                                                                    cTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGTAATTTGCGATTGCGGCCCGTTGTTTCTCGATGTAAATGGGGTTCTGCCGGTata                                           >  1:896532/1‑146 (MQ=255)
                                                                                                    cTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTGTTGAAATTTCCGATGGCGGCACGGTGTTTCTCGATGAAATTGGCGGTCTGCCGGTAGa                                           >  1:587008/1‑148 (MQ=255)
                                                                                                       ccATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTGTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACt                                        >  1:271816/1‑148 (MQ=255)
                                                                                                        cATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTTAAATTTCCGATGGCGGCACCTTTTTTGTCGATGTAATTTTCGATCTGCCGTTAGaaaat                                       >  1:308696/1‑145 (MQ=255)
                                                                                                        cATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACtt                                       <  2:2981981/148‑1 (MQ=255)
                                                                                                         aTCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGGTAGAACTTc                                      >  1:419516/1‑148 (MQ=255)
                                                                                                            cccGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGc                                   >  1:2089505/1‑148 (MQ=255)
                                                                                                            cccGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGc                                   <  2:3018700/148‑1 (MQ=255)
                                                                                                                ccAGTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTaa                               <  2:1611767/148‑1 (MQ=255)
                                                                                                                 cAGTCTTCTGGAAAGTGAGTTATTCGGTAATTACAAAGGGTCGTTTACTGGTGCGATTAATAACCAACGTGGACGTTTATAAATTGACGAAGTCGGCACCGTTTTTCTCGATTAAAAATGCCATCTCACCGTAGAACTACAGCCaaaa                              >  2:1439481/1‑148 (MQ=255)
                                                                                                                   gTCTTCTGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAAct                            >  1:2428260/1‑148 (MQ=255)
                                                                                                                     cttctGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAActgc                          <  2:1863032/148‑1 (MQ=255)
                                                                                                                       tctGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAActgctg                        <  2:2727127/148‑1 (MQ=255)
                                                                                                                       tctGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAActgctg                        >  2:342333/1‑148 (MQ=255)
                                                                                                                       tctGGAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAActgctg                        >  2:2143186/1‑148 (MQ=255)
                                                                                                                           gAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTGTCGATTAAATTTGCGATCTGCCGTTAGAACTTCAGCCTAAACAGCTGcgcg                    >  1:2035686/1‑148 (MQ=255)
                                                                                                                           gAAAGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATTAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGcgcg                    >  1:1348615/1‑148 (MQ=255)
                                                                                                                              aGTGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTAt                 <  2:1781151/148‑1 (MQ=255)
                                                                                                                                tGAGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTg               >  1:3007121/1‑148 (MQ=255)
                                                                                                                                  aGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCa             >  2:3132430/1‑148 (MQ=255)
                                                                                                                                  aGTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTGTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCa             >  2:1552235/1‑148 (MQ=255)
                                                                                                                                   gTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTTCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATATGCCGTGAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAg            >  1:2629103/1‑148 (MQ=255)
                                                                                                                                   gTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTTGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAg            >  2:2397601/1‑148 (MQ=255)
                                                                                                                                   gTTATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAg            >  2:1832064/1‑148 (MQ=255)
                                                                                                                                     tATTCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTTCCGATTGCGGCACCTTGTTTCTCGATGGAATTTGCGATCTTCCGTTAGAACTTAATCCAAAAATGCTGCCCGTATTTCAGGa          >  1:2810010/1‑148 (MQ=255)
                                                                                                                                        tCGGTCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCg       >  2:445054/1‑148 (MQ=255)
                                                                                                                                            tCATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTTAAATTTCCGAAGGCGGCACCTTGTTTATCGATGAAATTTGCGATATGCCGTTAGAAAATCAGCCAAAAAATCTGCGCGTATTTCAGGAGCGGgag   >  1:2850533/1‑148 (MQ=255)
                                                                                                                                             cATTACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGgaga  <  2:404404/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TTGATTTGTGGTGAAACCGGAACTGGCAAAGAGGTGATCGCCAGAGCGATCCATCAACTTAGCCCGCGACGCGACAAGCCGCTGGTCAAAATCAACTGCGCTGCCATCCCCGCCAGTCTTCTGGAAAGTGAGTTATTCGGTCATGACAAAGGGGCGTTTACTGGTGCGATTAATACCCATCGTGGTCGTTTTGAAATTGCCGATGGCGGCACGTTGTTTCTCGATGAAATTGGCGATCTGCCGTTAGAACTTCAGCCTAAACTGCTGCGCGTATTGCAGGAGCGGGAGA  >  CP009273/2606372‑2606660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑