BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,619,125	G→T	9.0%	G218C (GGT→TGT)	ppx →	exopolyphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,619,125	0	G	T	9.0%	624.4 / 26.5	178	G218C (GGT→TGT)	ppx	exopolyphosphatase
Reads supporting (aligned to +/- strand): ref base G (81/81); new base T (9/7); total (90/88)
Fisher's exact test for biased strand distribution p-value = 7.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 179 total aligned reads displayed.

TGTGTCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTC  >  CP009273/2618978‑2619264

    tCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTAc                                                                                                                                         >  2:1409947/1‑148 (MQ=255)

      aGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCa                                                                                                                                       >  1:780021/1‑148 (MQ=255)

       gCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCAt                                                                                                                                      >  2:28210/1‑148 (MQ=255)

       gCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCAt                                                                                                                                      >  2:2917138/1‑148 (MQ=255)

          ttGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATaaa                                                                                                                                   <  2:135410/148‑1 (MQ=255)

          ttGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATaaa                                                                                                                                   >  1:406504/1‑148 (MQ=255)

            gCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGAACAAAAACTGGAAACTTTAACCTGGCAATTCCGAATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCAGAAAAg                                                                                                                                 >  1:361506/1‑148 (MQ=255)

             cccAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAgc                                                                                                                                <  1:262379/148‑1 (MQ=255)

               cAGCTTTATTTTCCTGGCGGTGTCATCAATAAAGAGAATTTTCATCTCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAAAGTTGAAATGGGCGCTTCCGGTACCATAAAAgccg                                                                                                                              >  1:2992143/1‑148 (MQ=255)

                aGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAgccgc                                                                                                                             >  1:128813/1‑148 (MQ=255)

                aGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAgccgc                                                                                                                             >  1:1822684/1‑148 (MQ=255)

                     tATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATg                                                                                                                        >  1:1722860/1‑148 (MQ=255)

                      aTTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGa                                                                                                                       <  2:2955231/148‑1 (MQ=255)

                             tGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTa                                                                                                                >  2:2334547/1‑148 (MQ=255)

                             tGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTa                                                                                                                <  1:1815145/148‑1 (MQ=255)

                               gCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAAt                                                                                                              <  1:789832/148‑1 (MQ=255)

                                  gggTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGa                                                                                                           >  1:1933953/1‑148 (MQ=255)

                                      catcaATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATg                                                                                                       >  2:2431320/1‑148 (MQ=255)

                                       atcaATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATgg                                                                                                      >  2:1516056/1‑148 (MQ=255)

                                       atcaATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATgg                                                                                                      <  1:555487/148‑1 (MQ=255)

                                       atcaATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATgg                                                                                                      <  1:1059790/148‑1 (MQ=255)

                                        tgaaTAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATggg                                                                                                     >  2:1818782/3‑148 (MQ=255)

                                         caATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGc                                                                                                    >  1:1209394/1‑148 (MQ=255)

                                         caATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGc                                                                                                    >  1:2872833/1‑148 (MQ=255)

                                          aaTAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCg                                                                                                   <  2:3032832/148‑1 (MQ=255)

                                           aTAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCga                                                                                                  <  2:2487644/148‑1 (MQ=255)

                                             aaaGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCgaga                                                                                                >  1:2772008/1‑148 (MQ=255)

                                                 agaATTTTCAGCGCGCTCGCATGGCGTCAGCACAAAAACTGGAATCTTTAACCTGTCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGa                                                                                            <  1:2462069/148‑1 (MQ=255)

                                                 agaATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAAGGGAAATGGGCGAGAAAGa                                                                                            >  2:3031902/1‑148 (MQ=255)

                                                   aaTTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTGAGGGCTGGAACGTTGCAAGGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACg                                                                                          <  1:2784133/148‑1 (MQ=255)

                                                   aaTTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACg                                                                                          >  2:1873523/1‑148 (MQ=255)

                                                   aaTTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACg                                                                                          <  1:2714119/148‑1 (MQ=255)

                                                     ttttCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACggg                                                                                        <  1:1870850/148‑1 (MQ=255)

                                                      tttCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGa                                                                                       <  1:3260761/148‑1 (MQ=255)

                                                      tttCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGa                                                                                       <  1:74758/148‑1 (MQ=255)

                                                       ttCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGAt                                                                                      <  1:1873523/148‑1 (MQ=255)

                                                       ttCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGAt                                                                                      <  2:2714439/148‑1 (MQ=255)

                                                         cAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATaa                                                                                    <  1:1851780/148‑1 (MQ=255)

                                                         cAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATaa                                                                                    <  1:1566227/148‑1 (MQ=255)

                                                           gcgcgcTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAAtt                                                                                  <  2:729087/148‑1 (MQ=255)

                                                           gcgcgcTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAAtt                                                                                  <  2:2906993/148‑1 (MQ=255)

                                                            cgcgcTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGACATGGGCGAGAACGACGGGATAATTa                                                                                 >  2:1834703/1‑148 (MQ=255)

                                                            cgcgcTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTa                                                                                 >  2:1119428/1‑148 (MQ=255)

                                                              cgcTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTAcc                                                                               <  1:3275176/148‑1 (MQ=255)

                                                                 tCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCg                                                                            >  2:1237138/1‑148 (MQ=255)

                                                                    cATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGaa                                                                         <  1:1038526/148‑1 (MQ=255)

                                                                      tGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACg                                                                       <  1:3053901/148‑1 (MQ=255)

                                                                      tGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACg                                                                       >  2:500261/1‑148 (MQ=255)

                                                                      tGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACg                                                                       <  2:2234208/148‑1 (MQ=255)

                                                                      tGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACg                                                                       >  1:859223/1‑148 (MQ=255)

                                                                      tGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAAGGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACg                                                                       <  2:1147922/148‑1 (MQ=255)

                                                                       ggcggcAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGt                                                                      <  1:1225826/148‑1 (MQ=255)

                                                                            cagcaCAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGa                                                                 >  1:2401237/1‑148 (MQ=255)

                                                                               cacaAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGaaaa                                                              <  2:1135019/148‑1 (MQ=255)

                                                                                  aaaaaCTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACt                                                           <  2:2732106/148‑1 (MQ=255)

                                                                                   aaaaCTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTg                                                          >  2:3170459/1‑148 (MQ=255)

                                                                                     aaCTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGt                                                        >  1:1881812/1‑148 (MQ=255)

                                                                                     aaCTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGt                                                        >  2:1279131/1‑148 (MQ=255)

                                                                                     aaCTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGt                                                        <  2:1224648/148‑1 (MQ=255)

                                                                                       cTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTaa                                                      <  1:2214279/148‑1 (MQ=255)

                                                                                       cTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTaa                                                      <  1:170943/148‑1 (MQ=255)

                                                                                         ggAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTaaaa                                                    <  1:184822/148‑1 (MQ=255)

                                                                                           aaaCTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAaaga                                                  <  1:3238700/148‑1 (MQ=255)

                                                                                             aCTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAaagaag                                                >  2:2731995/1‑148 (MQ=255)

                                                                                             aCTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAaagaag                                                <  1:252032/148‑1 (MQ=255)

                                                                                             aCTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAaagaag                                                <  1:1920664/148‑1 (MQ=255)

                                                                                               tttAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGtt                                              >  2:2473229/1‑148 (MQ=255)

                                                                                               tttAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGtt                                              >  2:1785379/1‑148 (MQ=255)

                                                                                                  aaCCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTa                                           <  1:2791954/148‑1 (MQ=255)

                                                                                                     cTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGt                                        >  2:2505943/1‑148 (MQ=255)

                                                                                                       ggCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCa                                      >  2:1746915/1‑148 (MQ=255)

                                                                                                        gCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCAAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCAc                                     <  1:90590/148‑1 (MQ=255)

                                                                                                         cAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCAcc                                    >  2:1071526/1‑148 (MQ=255)

                                                                                                          aaTTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCg                                   >  1:3246005/1‑148 (MQ=255)

                                                                                                           aTTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGt                                  >  1:1751073/1‑148 (MQ=255)

                                                                                                            ttCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTa                                 <  2:1930107/148‑1 (MQ=255)

                                                                                                            ttCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTa                                 >  1:1396257/1‑148 (MQ=255)

                                                                                                            ttCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTa                                 <  1:1374680/148‑1 (MQ=255)

                                                                                                             tCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTaa                                >  1:2985881/1‑148 (MQ=255)

                                                                                                             tCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTaa                                >  1:2984707/1‑148 (MQ=255)

                                                                                                             tCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTaa                                >  2:353076/1‑148 (MQ=255)

                                                                                                             tCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTaa                                <  1:782367/148‑1 (MQ=255)

                                                                                                              ccGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTact                               >  2:3204659/1‑146 (MQ=255)

                                                                                                               cGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAAtt                              <  1:501594/148‑1 (MQ=255)

                                                                                                                 tATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTc                            >  2:1996779/1‑148 (MQ=255)

                                                                                                                 tATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTc                            >  1:1486829/1‑148 (MQ=255)

                                                                                                                  aTTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCg                           >  2:2348568/1‑148 (MQ=255)

                                                                                                                  aTTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCg                           <  1:2346040/148‑1 (MQ=255)

                                                                                                                       gggCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCg                      >  2:187013/1‑148 (MQ=255)

                                                                                                                              aaCGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGtt               <  1:617166/148‑1 (MQ=255)

                                                                                                                              aaCGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGtt               >  2:3065416/1‑148 (MQ=255)

                                                                                                                                     cAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCggg        >  2:2580239/1‑148 (MQ=255)

                                                                                                                                      aaTGGGCGCTTCCGGTATCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGt       <  2:52889/148‑1 (MQ=255)

                                                                                                                                        tGGGCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCt     <  1:2921636/148‑1 (MQ=255)

                                                                                                                                        tGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCt     <  2:1822806/148‑1 (MQ=255)

                                                                                                                                          ggCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCttt   <  1:2069645/148‑1 (MQ=255)

                                                                                                                                          ggCGCTTCCTGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCttt   <  1:2069517/148‑1 (MQ=255)

                                                                                                                                          ggCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCttt   >  1:2221847/1‑148 (MQ=255)

                                                                                                                                           gcgcTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTc  <  2:3285075/148‑1 (MQ=255)

                                                                                                                                           gcgcTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTc  <  1:2593183/148‑1 (MQ=255)

TGTGTCAGCTTTGCCCAGCTTTATTTTCCTGGCGGGGTCATCAATAAAGAGAATTTTCAGCGCGCTCGCATGGCGGCAGCACAAAAACTGGAAACTTTAACCTGGCAATTCCGTATTCAGGGCTGGAACGTTGCAATGGGCGCTTCCGGTACCATAAAAGCCGCCCATGAAGTGTTAATGGAAATGGGCGAGAAAGACGGGATAATTACCCCGGAACGTCTGGAAAAACTGGTAAAAGAAGTTTTACGTCACCGTAATTTCGCATCGCTGAGTTTACCGGGTCTTTC  >  CP009273/2618978‑2619264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`