Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,633,402 C→A 88.4% R178L (CGC→CTC)  hisS ← histidyl tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,633,4020CA88.4% 359.6 / 47.3 153R178L (CGC→CTC) hisShistidyl tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (13/5);  new base A (62/72);  total (76/77)
Fisher's exact test for biased strand distribution p-value = 4.63e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

Only 100 of 154 total aligned reads displayed.

TCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGC  >  CP009273/2633255‑2633546
                                                                                                                                                   |                                                                                                                                                
tCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATcgc                                                                                                                                                  >  2:1833596/1‑148 (MQ=255)
   ccTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGt                                                                                                                                               >  1:2902154/1‑148 (MQ=255)
   ccTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGt                                                                                                                                               <  1:1828101/148‑1 (MQ=255)
     tAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTaa                                                                                                                                             >  2:1627426/1‑148 (MQ=255)
     tAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTaa                                                                                                                                             >  1:2199330/1‑148 (MQ=255)
      aaTGCCGGTGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAAt                                                                                                                                            <  1:28067/148‑1 (MQ=255)
      aaTGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCCGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAAt                                                                                                                                            <  1:3196973/148‑1 (MQ=255)
      aaTGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAAt                                                                                                                                            <  1:28290/148‑1 (MQ=255)
          ccGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGgc                                                                                                                                        >  2:1312971/1‑148 (MQ=255)
               gCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTg                                                                                                                                   <  1:2401557/148‑1 (MQ=255)
                cgtcgtTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGc                                                                                                                                  <  2:2255317/148‑1 (MQ=255)
                cgtcgtTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGc                                                                                                                                  <  2:2152055/148‑1 (MQ=255)
                cgtcgtTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGc                                                                                                                                  <  2:907528/148‑1 (MQ=255)
                  tcgtTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCtt                                                                                                                                <  2:2606547/148‑1 (MQ=255)
                   cgtTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTc                                                                                                                               >  2:1645823/1‑148 (MQ=255)
                    gtTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTcc                                                                                                                              <  1:2201832/148‑1 (MQ=255)
                          aaGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGaa                                                                                                                        <  2:2552205/148‑1 (MQ=255)
                             cgcCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCCGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCg                                                                                                                     >  1:1734695/1‑148 (MQ=255)
                             cgcCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCg                                                                                                                     <  1:2945584/148‑1 (MQ=255)
                               ccTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGAt                                                                                                                   >  2:1120010/1‑148 (MQ=255)
                               ccTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGAt                                                                                                                   >  2:1119383/1‑148 (MQ=255)
                                 tGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATag                                                                                                                 >  1:209570/1‑148 (MQ=255)
                                  gCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGACCCGATaga                                                                                                                >  2:847899/1‑148 (MQ=255)
                                  gCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATaga                                                                                                                >  1:1790236/1‑148 (MQ=255)
                                   cACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATTCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATagag                                                                                                               >  1:2562747/1‑148 (MQ=255)
                                   cACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATagag                                                                                                               <  1:590795/148‑1 (MQ=255)
                                   cACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATagag                                                                                                               <  1:947580/148‑1 (MQ=255)
                                    aCTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGt                                                                                                              <  1:560013/148‑1 (MQ=255)
                                     cTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGtt                                                                                                             <  2:729736/148‑1 (MQ=255)
                                     cTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGtt                                                                                                             <  1:605457/148‑1 (MQ=255)
                                      ttCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCCAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTc                                                                                                            <  1:526068/148‑1 (MQ=255)
                                      ttCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTc                                                                                                            <  1:3292474/148‑1 (MQ=255)
                                      ttCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTc                                                                                                            <  1:3068193/148‑1 (MQ=255)
                                      ttCCGGATTTTGTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTc                                                                                                            <  1:3068235/148‑1 (MQ=255)
                                       tCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCa                                                                                                           >  1:1582338/1‑148 (MQ=255)
                                        ccGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTGTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGACTGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAg                                                                                                          >  2:249212/1‑148 (MQ=255)
                                            aTTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCa                                                                                                      >  2:1666437/1‑148 (MQ=255)
                                              tttttGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAag                                                                                                    <  2:2887655/148‑1 (MQ=255)
                                                tttGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAagag                                                                                                  <  1:1102476/148‑1 (MQ=255)
                                                tttGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAagag                                                                                                  <  1:1102333/148‑1 (MQ=255)
                                                  tGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGtt                                                                                                <  1:2962211/148‑1 (MQ=255)
                                                      tCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGt                                                                                            >  2:440546/1‑148 (MQ=255)
                                                        cAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGc                                                                                          >  2:536416/1‑148 (MQ=255)
                                                         aGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCt                                                                                         <  2:2674075/148‑1 (MQ=255)
                                                                 cAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATa                                                                                 <  2:200975/148‑1 (MQ=255)
                                                                 cAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATa                                                                                 <  2:1870816/148‑1 (MQ=255)
                                                                      ggTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAg                                                                            <  2:788306/148‑1 (MQ=255)
                                                                      ggTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAg                                                                            <  2:3025566/148‑1 (MQ=255)
                                                                         tAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAgcgc                                                                         >  1:1935629/1‑148 (MQ=255)
                                                                            tgtACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAgcgcgcg                                                                      >  1:1035044/1‑148 (MQ=255)
                                                                             gtACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAAATGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAgcgcgcgc                                                                     >  2:2030560/1‑148 (MQ=255)
                                                                               aCATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGcca                                                                   <  1:3038689/148‑1 (MQ=255)
                                                                                cATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGccac                                                                  <  2:1185573/148‑1 (MQ=255)
                                                                                cATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGccac                                                                  <  1:1645823/148‑1 (MQ=255)
                                                                                  tGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGccacca                                                                >  2:1476524/1‑148 (MQ=255)
                                                                                  tGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGccacca                                                                >  2:1475881/1‑148 (MQ=255)
                                                                                      gcgTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCgg                                                            <  1:2642517/148‑1 (MQ=255)
                                                                                         tttGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCa                                                         >  1:998572/1‑148 (MQ=255)
                                                                                         tttGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCa                                                         >  1:2655190/1‑148 (MQ=255)
                                                                                          ttGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAg                                                        <  1:339615/148‑1 (MQ=255)
                                                                                           tGCAGTCTTCGTCCCGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGt                                                       <  1:3245223/148‑1 (MQ=255)
                                                                                              aGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAg                                                    <  1:86784/148‑1 (MQ=255)
                                                                                                tCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCa                                                  >  1:731260/1‑148 (MQ=255)
                                                                                                tCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCa                                                  >  1:731362/1‑148 (MQ=255)
                                                                                                tCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCa                                                  <  1:1845588/148‑1 (MQ=255)
                                                                                                tCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCa                                                  >  1:1878649/1‑148 (MQ=255)
                                                                                                  ttCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATa                                                <  1:89768/148‑1 (MQ=255)
                                                                                                  ttCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATa                                                <  1:857993/148‑1 (MQ=255)
                                                                                                   tCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATaa                                               >  2:1270337/1‑148 (MQ=255)
                                                                                                     gTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATc                                             <  2:2937894/148‑1 (MQ=255)
                                                                                                       ccAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAg                                           >  2:304893/1‑148 (MQ=255)
                                                                                                        cAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTACTTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGt                                          >  1:1264287/1‑148 (MQ=255)
                                                                                                        cAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGt                                          >  1:1191192/1‑148 (MQ=255)
                                                                                                        cAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGt                                          >  1:2158170/1‑148 (MQ=255)
                                                                                                        cAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGt                                          >  2:680473/1‑148 (MQ=255)
                                                                                                          gCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTc                                        >  2:47252/1‑148 (MQ=255)
                                                                                                           cTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCa                                       >  2:506203/1‑148 (MQ=255)
                                                                                                           cTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCa                                       >  1:1500724/1‑148 (MQ=255)
                                                                                                             tttCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGc                                     >  2:1062566/1‑148 (MQ=255)
                                                                                                                   tATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGat                               <  2:3293716/148‑1 (MQ=255)
                                                                                                                   tATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGat                               <  1:1119383/148‑1 (MQ=255)
                                                                                                                   tATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGat                               <  1:1120010/148‑1 (MQ=255)
                                                                                                                     tgctgcTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGatat                             >  1:1647235/1‑148 (MQ=255)
                                                                                                                            cAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGAcc                      <  1:1259169/148‑1 (MQ=255)
                                                                                                                               ggAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTg                   >  2:3194728/1‑148 (MQ=255)
                                                                                                                                gAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGc                  <  2:2288649/148‑1 (MQ=255)
                                                                                                                                gAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGc                  >  1:1098215/1‑148 (MQ=255)
                                                                                                                                gAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGc                  >  2:1539466/1‑148 (MQ=255)
                                                                                                                                    gCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGAc              >  1:2238156/1‑148 (MQ=255)
                                                                                                                                       accaGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGa           >  1:1366346/1‑148 (MQ=255)
                                                                                                                                          aGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGaaaa        <  2:3281667/148‑1 (MQ=255)
                                                                                                                                          aGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGaaaa        >  1:2700379/1‑148 (MQ=255)
                                                                                                                                            cgcATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACt      >  1:2896279/1‑148 (MQ=255)
                                                                                                                                             gcATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACtt     <  1:440546/148‑1 (MQ=255)
                                                                                                                                              cATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTc    <  1:1177419/148‑1 (MQ=255)
                                                                                                                                                tCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGc  >  1:782055/1‑148 (MQ=255)
                                                                                                                                                tCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGc  >  1:782176/1‑148 (MQ=255)
                                                                                                                                                tCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGc  >  2:2023444/1‑148 (MQ=255)
                                                                                                                                                tCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGc  >  2:208982/1‑148 (MQ=255)
                                                                                                                                                tCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTAAGTGCTCGGAAATACCCATCGCGCGCCACCAGCGGGCAGAGAGCATAATCAGTTCAGCGACGATATCCGGACCATGCAGACCGAAAACTACGc  >  1:2377479/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TCACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTCGC  >  CP009273/2633255‑2633546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑