Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,660,155 G→A 17.0% Q351* (CAA→TAA)  hcaT ← putative 3‑phenylpropionic transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,660,1550GA17.0% 465.0 / 72.7 170Q351* (CAA→TAA) hcaTputative 3‑phenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base G (65/76);  new base A (13/16);  total (78/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 170 total aligned reads displayed.

ATACGGACCTGACGCTGACAACACATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTG  >  CP009273/2660010‑2660300
                                                                                                                                                 |                                                                                                                                                 
ataCGGACCTGACGCTGACAACACATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGAt                                                                                                                                                 <  1:2501561/148‑1 (MQ=255)
ataCGGACCTGACGCTGACAACACATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGAt                                                                                                                                                 <  1:2618104/148‑1 (MQ=255)
ataCGGACCTGACGCTGACAACACATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGAt                                                                                                                                                 >  2:1759575/1‑148 (MQ=255)
        cTGACGCTGACAACACATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGaaa                                                                                                                                         >  2:2446005/1‑148 (MQ=255)
        cTGACGCTGACAACACATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGaaa                                                                                                                                         <  1:2478761/148‑1 (MQ=255)
        cTGACGCTGACAACACATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTAATACAGGaaa                                                                                                                                         <  2:2124407/148‑1 (MQ=255)
            cGCTGACAACACATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCgg                                                                                                                                     <  1:1823576/148‑1 (MQ=255)
                 acaacaCATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGaaa                                                                                                                                <  2:448543/148‑1 (MQ=255)
                   aacaCATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAAc                                                                                                                              <  1:2508841/148‑1 (MQ=255)
                    acacATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACg                                                                                                                             <  2:34092/148‑1 (MQ=255)
                      acaTCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAAAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGt                                                                                                                           <  2:1907562/148‑1 (MQ=255)
                             cAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATa                                                                                                                    >  2:828161/1‑148 (MQ=255)
                             cAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATa                                                                                                                    >  2:828369/1‑148 (MQ=255)
                             cAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATa                                                                                                                    <  2:2551019/148‑1 (MQ=255)
                              aGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAg                                                                                                                   <  1:252903/148‑1 (MQ=255)
                                gAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCg                                                                                                                 <  2:772673/148‑1 (MQ=255)
                                gAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCg                                                                                                                 <  1:2492707/148‑1 (MQ=255)
                                gAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGAGATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCg                                                                                                                 >  1:1960577/1‑148 (MQ=255)
                                 aGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGa                                                                                                                <  1:1575008/148‑1 (MQ=255)
                                 aGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTAATACAGGAAACCGGCGAAAACGGTCATGATAGCGa                                                                                                                >  2:360058/1‑148 (MQ=255)
                                  gCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGAt                                                                                                               <  1:1435094/148‑1 (MQ=255)
                                  gCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGAt                                                                                                               <  1:2176780/148‑1 (MQ=255)
                                   cAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATa                                                                                                              <  2:2332870/148‑1 (MQ=255)
                                    aGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATAc                                                                                                             <  2:2724187/148‑1 (MQ=255)
                                      aTCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTg                                                                                                           <  2:1358609/148‑1 (MQ=255)
                                      aTCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTg                                                                                                           >  2:956239/1‑148 (MQ=255)
                                      aTCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTg                                                                                                           >  2:2610155/1‑148 (MQ=255)
                                              aaTGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCAt                                                                                                   <  2:2926443/148‑1 (MQ=255)
                                               aTGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATc                                                                                                  >  2:1115097/1‑148 (MQ=255)
                                                tGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATcg                                                                                                 >  2:2288796/1‑148 (MQ=255)
                                                tGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATcg                                                                                                 >  2:798734/1‑148 (MQ=255)
                                                    cGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGAc                                                                                             <  2:170652/148‑1 (MQ=255)
                                                      aaTCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACgg                                                                                           <  2:668581/148‑1 (MQ=255)
                                                       aTCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTAATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGc                                                                                          >  1:2326872/1‑148 (MQ=255)
                                                         cAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCag                                                                                        >  2:2260739/1‑148 (MQ=255)
                                                          aGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCaga                                                                                       <  1:2715136/148‑1 (MQ=255)
                                                            cATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGt                                                                                     >  1:1526549/1‑148 (MQ=255)
                                                               gAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTaaa                                                                                  <  1:3063503/148‑1 (MQ=255)
                                                                  ggAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCg                                                                               >  1:1409772/1‑148 (MQ=255)
                                                                  ggAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCg                                                                               >  1:2312563/1‑148 (MQ=255)
                                                                    aacaacTTTCGGGCGCACAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCTCCCATCGCGACGGCAGAGTAAACCGcc                                                                             <  2:504932/148‑1 (MQ=255)
                                                                    aacaacTTTCGGGCGCAAACACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACTCGCCGTGGCCCAGATATTAATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCTGAGTAAACCGcc                                                                             <  2:158913/148‑1 (MQ=255)
                                                                    aacaacTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGcc                                                                             <  2:327153/148‑1 (MQ=255)
                                                                     acaacTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGcat                                                                            <  2:587484/148‑3 (MQ=255)
                                                                      caacTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTg                                                                           >  1:3006838/1‑148 (MQ=255)
                                                                         cTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTaa                                                                        >  1:2473438/1‑148 (MQ=255)
                                                                           ttCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTAATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAAc                                                                      <  2:2189874/148‑1 (MQ=255)
                                                                             cGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACgg                                                                    <  1:860959/148‑1 (MQ=255)
                                                                             cGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACgg                                                                    <  2:1299731/148‑1 (MQ=255)
                                                                                   cAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATAGAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACt                                                              >  2:1788500/1‑148 (MQ=255)
                                                                                   cAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACt                                                              <  1:1884133/148‑1 (MQ=255)
                                                                                   cAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACt                                                              <  2:3041821/148‑1 (MQ=255)
                                                                                   cAAAAACAGTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACt                                                              <  1:1750934/148‑1 (MQ=255)
                                                                                    aaaaaCATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACtt                                                             <  1:1759575/148‑1 (MQ=255)
                                                                                    aaaaaCATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTAATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACtt                                                             <  2:2645983/148‑1 (MQ=255)
                                                                                      aaaCATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCg                                                           >  2:840646/1‑148 (MQ=255)
                                                                                       aaCATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGc                                                          >  2:725932/1‑148 (MQ=255)
                                                                                       aaCATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGc                                                          <  1:1356608/148‑1 (MQ=255)
                                                                                        aCATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCt                                                         >  1:122794/1‑148 (MQ=255)
                                                                                        aCATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTAATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCt                                                         <  1:1012567/148‑1 (MQ=255)
                                                                                         cATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTa                                                        >  2:1814230/1‑148 (MQ=255)
                                                                                         cATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTa                                                        <  1:1064389/148‑1 (MQ=255)
                                                                                           ttGCCGTAAGCGCCACCCGCGCCATTCCCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGTCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTAcc                                                      <  2:249355/148‑1 (MQ=255)
                                                                                           ttGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTAcc                                                      <  1:1814230/148‑1 (MQ=255)
                                                                                           ttGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTAcc                                                      <  1:1814198/148‑1 (MQ=255)
                                                                                           ttGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTAcc                                                      >  1:2359535/1‑148 (MQ=255)
                                                                                           ttGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTAATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTAcc                                                      >  1:126858/1‑148 (MQ=255)
                                                                                           ttGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTAATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTAcc                                                      >  1:1802389/1‑148 (MQ=255)
                                                                                                 gAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCg                                                >  1:2175576/1‑148 (MQ=255)
                                                                                                  aaGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGa                                               <  1:2843375/148‑1 (MQ=255)
                                                                                                  aaGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGa                                               >  2:369043/1‑148 (MQ=255)
                                                                                                   aGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGag                                              <  2:674113/148‑1 (MQ=255)
                                                                                                    gcgcCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGagc                                             <  2:466447/148‑1 (MQ=255)
                                                                                                    gcgcCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTAATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGagc                                             >  1:1583463/1‑148 (MQ=255)
                                                                                                       ccaccaGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTaaa                                                                       <  2:3128339/119‑1 (MQ=255)
                                                                                                        caccaGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAgcagca                                         <  1:1517978/148‑1 (MQ=255)
                                                                                                        caccaGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTAATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAgcagca                                         <  2:1906250/148‑1 (MQ=255)
                                                                                                        caccaGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTAATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAgcagca                                         <  1:2910376/148‑1 (MQ=255)
                                                                                                          ccaGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAat                                       >  1:2075757/1‑148 (MQ=255)
                                                                                                          ccaGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAat                                       >  1:458111/1‑148 (MQ=255)
                                                                                                              cgcCATTACCCAGAACACGCCGTGGCCCAGATATTAATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATaa                                   >  1:323521/1‑148 (MQ=255)
                                                                                                                 cATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGc                                <  1:2610155/148‑1 (MQ=255)
                                                                                                                 cATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGc                                <  1:494115/148‑1 (MQ=255)
                                                                                                                 cATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGc                                <  1:956239/148‑1 (MQ=255)
                                                                                                                   ttACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCAt                              <  2:65574/148‑1 (MQ=255)
                                                                                                                    tACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATg                             >  1:1479880/1‑148 (MQ=255)
                                                                                                                     aCCCAGAACACGCCGTGGCCCAGATATTAATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATgg                            <  1:2828192/148‑1 (MQ=255)
                                                                                                                         aGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATggcggc                        >  2:1617699/1‑148 (MQ=255)
                                                                                                                          gAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGcc                       >  2:301240/1‑148 (MQ=255)
                                                                                                                            acacGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAg                     >  1:2277415/1‑148 (MQ=255)
                                                                                                                            acacGCCGTGGCCCAGATATTAATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAg                     >  1:2031095/1‑148 (MQ=255)
                                                                                                                             cacGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAgg                    >  1:2430676/1‑148 (MQ=255)
                                                                                                                                   gTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAg              >  1:437139/1‑148 (MQ=255)
                                                                                                                                    tGGCCCAGATATTAATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGa             <  2:1565635/148‑1 (MQ=255)
                                                                                                                                          aGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGa       <  1:2921719/148‑1 (MQ=255)
                                                                                                                                            atatTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAg     <  1:2769208/148‑1 (MQ=255)
                                                                                                                                            atatTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAg     >  2:1445575/1‑148 (MQ=255)
                                                                                                                                            atatTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAg     >  1:1012850/1‑148 (MQ=255)
                                                                                                                                              atTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGt   >  2:2734744/1‑148 (MQ=255)
                                                                                                                                              atTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGt   >  2:2594866/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
ATACGGACCTGACGCTGACAACACATCAACAGGAGCAGATCCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTG  >  CP009273/2660010‑2660300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑