Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,669,978 C→A 90.2% A69A (GCG→GCT)  yphD ← inner membrane putative ABC superfamily sugar transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,669,9780CA90.2% 492.4 / 48.0 192A69A (GCG→GCT) yphDinner membrane putative ABC superfamily sugar transporter permease
Reads supporting (aligned to +/- strand):  ref base C (14/5);  new base A (84/89);  total (98/94)
Fisher's exact test for biased strand distribution p-value = 5.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

Only 100 of 192 total aligned reads displayed.

TCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCGA  >  CP009273/2669833‑2670122
                                                                                                                                                 |                                                                                                                                                
tCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGc                                                                                                                                                >  1:2098355/1‑148 (MQ=255)
tCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGc                                                                                                                                                >  2:611345/1‑148 (MQ=255)
 cAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGcc                                                                                                                                               >  1:564400/1‑148 (MQ=255)
 cAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGcc                                                                                                                                               <  1:2269618/148‑1 (MQ=255)
   gcgcgcCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCa                                                                                                                                             <  2:750623/148‑1 (MQ=255)
     gcgcCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAgg                                                                                                                                           <  1:2435882/148‑1 (MQ=255)
     gcgcCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAgg                                                                                                                                           <  1:2435886/148‑1 (MQ=255)
       gcCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAggcg                                                                                                                                         <  2:3236462/148‑1 (MQ=255)
        ccTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAggcgg                                                                                                                                        <  1:566568/148‑1 (MQ=255)
        ccTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAggcgg                                                                                                                                        <  2:563983/148‑1 (MQ=255)
                cAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCa                                                                                                                                <  2:131300/148‑1 (MQ=255)
                cAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAAGTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAAGTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCa                                                                                                                                <  2:1803327/148‑1 (MQ=255)
                 aaCACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCaa                                                                                                                               <  2:309054/148‑1 (MQ=255)
                   cacCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAtg                                                                                                                             >  1:280346/1‑148 (MQ=255)
                   cacCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAtg                                                                                                                             >  1:2492349/1‑148 (MQ=255)
                   cacCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAtg                                                                                                                             >  1:375721/1‑148 (MQ=255)
                   cacCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAtg                                                                                                                             >  1:2042041/1‑148 (MQ=255)
                           ggCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTggcggc                                                                                                                     <  1:104709/148‑1 (MQ=255)
                             cACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCAt                                                                                                                   <  2:21928/148‑1 (MQ=255)
                                  cACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCgcgc                                                                                                              <  2:2748442/148‑1 (MQ=255)
                                    ccGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACCTCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCgcag                                                                                                            >  2:2111550/1‑148 (MQ=255)
                                    ccGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCgcag                                                                                                            >  1:1013439/1‑148 (MQ=255)
                                     cGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCgcagc                                                                                                           >  2:529485/1‑148 (MQ=255)
                                         aGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGt                                                                                                       <  1:1058359/148‑1 (MQ=255)
                                          gCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGtt                                                                                                      >  2:129252/1‑148 (MQ=255)
                                             gAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCAt                                                                                                   <  1:2896514/148‑1 (MQ=255)
                                             gAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCAt                                                                                                   <  2:3024604/148‑1 (MQ=255)
                                             gAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCAt                                                                                                   >  1:2999082/1‑148 (MQ=255)
                                             gAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCAt                                                                                                   >  1:2384401/1‑148 (MQ=255)
                                             gAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCAt                                                                                                   <  2:2593554/148‑1 (MQ=255)
                                             gAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCAt                                                                                                   >  2:2640407/1‑148 (MQ=255)
                                              aaCTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATc                                                                                                  <  2:1324909/148‑1 (MQ=255)
                                                cTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTg                                                                                                >  2:1229990/1‑148 (MQ=255)
                                                cTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTg                                                                                                >  1:2729057/1‑148 (MQ=255)
                                                cTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTg                                                                                                >  1:632821/1‑148 (MQ=255)
                                                cTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTg                                                                                                >  2:2429192/1‑148 (MQ=255)
                                                 ttCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTgg                                                                                               >  1:2360516/1‑148 (MQ=255)
                                                   cAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGtt                                                                                             <  1:771710/148‑1 (MQ=255)
                                                   cAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGtt                                                                                             <  1:916472/148‑1 (MQ=255)
                                                    aaaTTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTa                                                                                            <  1:417715/148‑1 (MQ=255)
                                                      aTTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTAtt                                                                                          >  1:2507325/1‑148 (MQ=255)
                                                       ttGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTc                                                                                         >  2:1257071/1‑148 (MQ=255)
                                                        tGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCa                                                                                        <  2:1946558/148‑1 (MQ=255)
                                                         gcagcaAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCaa                                                                                       <  1:1848118/148‑1 (MQ=255)
                                                         gcagcaAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCaa                                                                                       <  2:1046035/148‑1 (MQ=255)
                                                             caAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATgag                                                                                   >  2:1982690/1‑148 (MQ=255)
                                                             caAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATgag                                                                                   >  1:2784438/1‑148 (MQ=255)
                                                                aaaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATa                                                                                >  1:2630127/1‑148 (MQ=255)
                                                                    gCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGc                                                                            <  2:2953059/148‑1 (MQ=255)
                                                                    gCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGc                                                                            <  1:771313/148‑1 (MQ=255)
                                                                    gCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGc                                                                            <  2:1899944/148‑1 (MQ=255)
                                                                    gCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGc                                                                            <  2:270356/148‑1 (MQ=255)
                                                                       aGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAg                                                                         >  2:2252273/1‑148 (MQ=255)
                                                                         gCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTAATATACCAGGCGTCAATCCCAAATGTGGCGGCATCGCGCAGCACGTTAATCTGGTTATTAAATGAGATAAAGACAGgc                                                                       >  2:47940/1‑148 (MQ=255)
                                                                               cGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTc                                                                 >  2:1644728/1‑148 (MQ=255)
                                                                                gagaCAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCa                                                                <  2:428886/148‑1 (MQ=255)
                                                                                 agaCAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAg                                                               >  1:1893803/1‑148 (MQ=255)
                                                                                 agaCAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAg                                                               >  1:1119489/1‑148 (MQ=255)
                                                                                        aGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGagaag                                                        >  1:3212518/1‑148 (MQ=255)
                                                                                            accaTCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCa                                                    >  1:305587/1‑148 (MQ=255)
                                                                                                 cGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATaa                                               >  1:1379913/1‑148 (MQ=255)
                                                                                                      cAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAAt                                          >  2:1910953/1‑148 (MQ=255)
                                                                                                      cAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAAt                                          >  1:2323199/1‑148 (MQ=255)
                                                                                                        aCGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATgg                                        >  1:2811987/1‑148 (MQ=255)
                                                                                                         cGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGc                                       >  1:1463281/1‑148 (MQ=255)
                                                                                                           cTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCaa                                     >  2:1924423/1‑148 (MQ=255)
                                                                                                            tGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAAt                                    >  2:2424066/1‑148 (MQ=255)
                                                                                                                aTCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATcacc                                <  1:1354708/148‑1 (MQ=255)
                                                                                                                         aCCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCaaa                       <  1:2429192/148‑1 (MQ=255)
                                                                                                                         aCCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCaaa                       >  2:1258915/1‑148 (MQ=255)
                                                                                                                          ccGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAAc                      >  1:2956118/1‑148 (MQ=255)
                                                                                                                          ccGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAAc                      <  2:2780367/148‑1 (MQ=255)
                                                                                                                          ccGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAAc                      >  1:2125378/1‑148 (MQ=255)
                                                                                                                          ccGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAAc                      >  1:2125270/1‑148 (MQ=255)
                                                                                                                            ggAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCg                    >  1:3019584/1‑148 (MQ=255)
                                                                                                                              agaTAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATCAAGAATGGCAATCACCACCAGCAAACCGAt                  >  1:282807/1‑148 (MQ=255)
                                                                                                                              agaTAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAt                  <  2:1879416/148‑1 (MQ=255)
                                                                                                                              agaTAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAt                  >  1:1151360/1‑148 (MQ=255)
                                                                                                                                ataataATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAtct                >  2:1103762/1‑148 (MQ=255)
                                                                                                                                 taataaTCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAtctc               <  1:1233223/148‑1 (MQ=255)
                                                                                                                                 taataaTCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAtctc               <  1:1547353/148‑1 (MQ=255)
                                                                                                                                 taataaTCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAtctc               <  1:3187683/148‑1 (MQ=255)
                                                                                                                                  aataatCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCa              >  1:1447754/1‑148 (MQ=255)
                                                                                                                                  aataatCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCa              <  2:1493852/148‑1 (MQ=255)
                                                                                                                                     aatCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTa           >  2:885837/1‑148 (MQ=255)
                                                                                                                                     aatCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTa           >  2:2276747/1‑148 (MQ=255)
                                                                                                                                     aatCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTa           <  1:3053393/148‑1 (MQ=255)
                                                                                                                                      ctCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGTCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCAACAGCAAACCGATCTCATTaa          <  2:2333006/147‑1 (MQ=255)
                                                                                                                                      atCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTaa          <  2:375721/148‑1 (MQ=255)
                                                                                                                                      atCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTaa          <  2:2042041/148‑1 (MQ=255)
                                                                                                                                      atCAGCGTCATAGCCAAGGCTGCAATCCAAATGGAGTCGGCATCGCGCAGCACGTTCATCTGGTTAATCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGTCAAACACCACCAGCAAACCGATCTCATTaa          >  2:1588953/1‑148 (MQ=255)
                                                                                                                                       tCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAat         >  2:3039293/1‑148 (MQ=255)
                                                                                                                                        cAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAata        >  1:706096/1‑148 (MQ=255)
                                                                                                                                         aGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAatat       >  2:1669654/1‑148 (MQ=255)
                                                                                                                                         aGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAatat       <  1:1644728/148‑1 (MQ=255)
                                                                                                                                         aGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAatat       <  2:280346/148‑1 (MQ=255)
                                                                                                                                         aGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAatat       <  2:2492355/148‑1 (MQ=255)
                                                                                                                                           cGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATgg     >  1:1127727/1‑148 (MQ=255)
                                                                                                                                             tCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCg   <  1:1841246/148‑1 (MQ=255)
                                                                                                                                              cATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCGa  <  1:3132149/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCGA  >  CP009273/2669833‑2670122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑