Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,675,664 T→G 9.4% V147V (GTA→GTC)  yphG ← DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,675,6640TG 9.4% 392.0 / 35.5 149V147V (GTA→GTC) yphGDUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base T (65/70);  new base G (7/7);  total (72/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

Only 100 of 152 total aligned reads displayed.

TTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATG  >  CP009273/2675518‑2675811
                                                                                                                                                  |                                                                                                                                                   
ttCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTa                                                                                                                                                    <  1:1400772/148‑1 (MQ=255)
ttCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTa                                                                                                                                                    <  1:2785006/148‑1 (MQ=255)
  cACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTAcc                                                                                                                                                  <  2:559318/148‑1 (MQ=255)
    cTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCca                                                                                                                                                >  2:1613458/1‑148 (MQ=255)
      gCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCcaca                                                                                                                                              <  2:1455311/148‑1 (MQ=255)
      gCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCcaca                                                                                                                                              <  2:1455701/148‑1 (MQ=255)
       ccGGGTTGGCCCACCACAAGAAATGTCGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCacac                                                                                                                                             >  2:2625432/1‑148 (MQ=255)
           gTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTc                                                                                                                                         <  2:326076/148‑1 (MQ=255)
               gCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCtgtg                                                                                                                                     <  1:104442/148‑1 (MQ=255)
               gCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCtgtg                                                                                                                                     >  2:1314949/1‑148 (MQ=255)
               gCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCGACCCACACCGTCtgtg                                                                                                                                     >  1:864644/1‑148 (MQ=255)
                  caccacAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCAc                                                                                                                                  >  2:15067/1‑148 (MQ=255)
                  caccacAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCAc                                                                                                                                  >  2:2508530/1‑148 (MQ=255)
                   accacAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCAcc                                                                                                                                 >  1:3282830/1‑148 (MQ=255)
                   accacAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCAcc                                                                                                                                 >  2:2270457/1‑148 (MQ=255)
                    ccacAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCg                                                                                                                                >  1:1668824/1‑148 (MQ=255)
                       caAGAAATGACGCGGCGTGGCGGTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCt                                                                                                                             <  2:2423539/148‑1 (MQ=255)
                          gAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCa                                                                                                                          <  1:1803832/148‑1 (MQ=255)
                          gAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCa                                                                                                                          <  1:1803836/148‑1 (MQ=255)
                             aTGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATgg                                                                                                                       >  1:303760/1‑148 (MQ=255)
                              tGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATggg                                                                                                                      >  1:2896029/1‑148 (MQ=255)
                                 cgcgGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCtt                                                                                                                   >  2:291037/1‑148 (MQ=255)
                                   cggcgTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCg                                                                                                                 >  1:576008/1‑148 (MQ=255)
                                   cggcgTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCGACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCg                                                                                                                 <  1:2371867/148‑1 (MQ=255)
                                    ggcgtggcgtTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGa                                                                                                                <  1:1858131/148‑1 (MQ=255)
                                    ggcgtggcgtTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCGACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGa                                                                                                                <  2:1858267/148‑1 (MQ=255)
                                      cgtggcgtTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAgg                                                                                                              <  1:1971822/148‑1 (MQ=255)
                                      cgtggcgtTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAgg                                                                                                              <  1:460566/148‑1 (MQ=255)
                                      cgtggcgtTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAgg                                                                                                              >  2:1206172/1‑148 (MQ=255)
                                      cgtggcgtTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAgg                                                                                                              >  2:1206073/1‑148 (MQ=255)
                                      cgtggcgtTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAgg                                                                                                              <  1:2779796/148‑1 (MQ=255)
                                       gtggcgtTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAggg                                                                                                             <  2:1035909/148‑1 (MQ=255)
                                         ggcgtTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTg                                                                                                           >  1:2304589/1‑148 (MQ=255)
                                         ggcgtTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTg                                                                                                           <  2:811661/148‑1 (MQ=255)
                                         ggcgtTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTg                                                                                                           >  1:2304583/1‑148 (MQ=255)
                                              tCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATc                                                                                                      >  2:526251/1‑148 (MQ=255)
                                               cccGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCa                                                                                                     >  1:1995587/1‑148 (MQ=255)
                                               cccGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCa                                                                                                     >  1:1995584/1‑148 (MQ=255)
                                                ccGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCaa                                                                                                    <  2:919218/148‑1 (MQ=255)
                                                ccGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCaa                                                                                                    <  1:2181677/148‑1 (MQ=255)
                                                 cGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAAc                                                                                                   <  1:3048058/148‑1 (MQ=255)
                                                 cGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAAc                                                                                                   <  2:472969/148‑1 (MQ=255)
                                                   ttATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCGTCGAGGGTGAAATCACCgg                                                                                                 >  2:3173245/1‑148 (MQ=255)
                                                    tataGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCGACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACggg                                                                                                <  2:2987761/148‑1 (MQ=255)
                                                    tataGACGCGGCTGGCGAGTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACggg                                                                                                <  2:683199/148‑1 (MQ=255)
                                                       aGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCAt                                                                                             >  1:1109143/1‑148 (MQ=255)
                                                       aGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCAt                                                                                             >  1:2082346/1‑148 (MQ=255)
                                                       aGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCAt                                                                                             >  1:2081377/1‑148 (MQ=255)
                                                         aCGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCAGaa                                                                                           >  1:887117/1‑148 (MQ=255)
                                                             ggCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAggt                                                                                       <  2:1913956/148‑1 (MQ=255)
                                                              gCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAggtg                                                                                      <  2:2396069/148‑1 (MQ=255)
                                                                 ggCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTc                                                                                   >  2:410842/1‑148 (MQ=255)
                                                                 ggCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTc                                                                                   <  1:2579368/148‑1 (MQ=255)
                                                                 ggCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTc                                                                                   >  2:453298/1‑148 (MQ=255)
                                                                 ggCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTc                                                                                   >  1:781200/1‑148 (MQ=255)
                                                                   cGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCgg                                                                                 <  1:3020983/148‑1 (MQ=255)
                                                                       ttCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCga                                                                             <  1:1240708/148‑1 (MQ=255)
                                                                          cAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCgatga                                                                          <  2:2584293/148‑1 (MQ=255)
                                                                             cgccgcCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCGACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGt                                                                       <  1:2508944/148‑1 (MQ=255)
                                                                               ccgccCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTg                                                                     <  1:2289358/148‑1 (MQ=255)
                                                                                       tCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGt                                                             >  2:1049642/1‑148 (MQ=255)
                                                                                        cAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGtt                                                            >  1:79591/1‑148 (MQ=255)
                                                                                        cAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGtt                                                            >  1:2219780/1‑148 (MQ=255)
                                                                                             cgcAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACt                                                       <  1:266477/148‑1 (MQ=255)
                                                                                             cgcAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACt                                                       <  1:266675/148‑1 (MQ=255)
                                                                                                 gggTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAAt                                                   <  1:918974/148‑1 (MQ=255)
                                                                                                  ggTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATc                                                  >  2:2545981/1‑148 (MQ=255)
                                                                                                   gTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATcc                                                 <  1:354695/148‑1 (MQ=255)
                                                                                                   gTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATcc                                                 <  1:555365/148‑1 (MQ=255)
                                                                                                     gAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAACGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCa                                               >  2:3298919/1‑148 (MQ=255)
                                                                                                         ccTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCgg                                           >  1:1819225/1‑148 (MQ=255)
                                                                                                          cTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGa                                          <  1:3223500/148‑1 (MQ=255)
                                                                                                          cTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGa                                          <  1:2522482/148‑1 (MQ=255)
                                                                                                           tGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGaa                                         >  2:1535351/1‑148 (MQ=255)
                                                                                                             tcatcaCCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAAt                                       <  1:1548983/148‑1 (MQ=255)
                                                                                                             tcatcaCCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAAt                                       <  1:2328677/148‑1 (MQ=255)
                                                                                                             tcatcaCCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAAt                                       <  1:2328726/148‑1 (MQ=255)
                                                                                                               atcaCCTGTAAACCATGCATCGGCTCCGTTTCGCCGACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATcc                                     >  2:1514340/1‑148 (MQ=255)
                                                                                                                tcaCCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCa                                    >  2:1326731/1‑148 (MQ=255)
                                                                                                                tcaCCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCa                                    >  1:1945787/1‑148 (MQ=255)
                                                                                                                tcaCCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCa                                    <  2:1570333/148‑1 (MQ=255)
                                                                                                                 caCCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCAc                                   >  2:1891768/1‑148 (MQ=255)
                                                                                                                 caCCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCAc                                   >  2:1978010/1‑148 (MQ=255)
                                                                                                                  aCCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACg                                  <  1:504247/148‑1 (MQ=255)
                                                                                                                     tGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTGGAGGGTGAAATCAACGGGCATAATGGGGGTCGGGCGATGATGTTGCGGCCATTTAAACTCAATCCCACCGGAAATCCACGGcc                               <  1:291037/148‑1 (MQ=255)
                                                                                                                     tGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGcc                               <  2:629548/148‑1 (MQ=255)
                                                                                                                     tGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGcc                               >  2:370987/1‑148 (MQ=255)
                                                                                                                     tGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGcc                               >  2:114302/1‑148 (MQ=255)
                                                                                                                      gTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGccc                              <  2:1784790/148‑1 (MQ=255)
                                                                                                                             aTGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCagcagc                       >  2:1687826/1‑148 (MQ=255)
                                                                                                                                   cGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCccacc                 >  2:2537640/1‑148 (MQ=255)
                                                                                                                                   cGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCccacc                 >  2:1714981/1‑148 (MQ=255)
                                                                                                                                      cTCCGTTTCGCCGACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAgc              >  2:205166/1‑148 (MQ=255)
                                                                                                                                           tgtCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAgg         <  2:1055882/146‑1 (MQ=255)
                                                                                                                                            ttCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGt        >  2:248937/1‑148 (MQ=255)
                                                                                                                                               gCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTa     <  1:2270457/148‑1 (MQ=255)
                                                                                                                                               gCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTa     <  2:781200/148‑1 (MQ=255)
                                                                                                                                                ccTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTaa    <  1:2362856/148‑1 (MQ=255)
                                                                                                                                                  tACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAACGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATg  <  2:1945787/148‑1 (MQ=255)
                                                                                                                                                  tACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATg  <  2:3171340/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGCTCCGTTTCGCCTACCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATG  >  CP009273/2675518‑2675811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑