Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,683,036 C→A 12.5% T474T (ACG→ACT)  glrK ← sensor protein kinase regulating glmY sRNA in two‑component system with response regulator GlrR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,683,0360CA12.5% 654.6 / 48.1 199T474T (ACG→ACT) glrKsensor protein kinase regulating glmY sRNA in two‑component system with response regulator GlrR
Reads supporting (aligned to +/- strand):  ref base C (88/86);  new base A (8/17);  total (96/103)
Fisher's exact test for biased strand distribution p-value = 9.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

Only 100 of 200 total aligned reads displayed.

CAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCCTTTTCGCTGG  >  CP009273/2682890‑2683183
                                                                                                                                                  |                                                                                                                                                   
cAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCg                                                                                                                                                    <  1:968540/148‑1 (MQ=255)
cAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCg                                                                                                                                                    <  2:505679/148‑1 (MQ=255)
 aGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCgt                                                                                                                                                   >  1:2456605/1‑148 (MQ=255)
   tGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCgtgt                                                                                                                                                 >  2:172580/1‑148 (MQ=255)
    gggCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGtt                                                                                                                                                <  1:1100247/148‑1 (MQ=255)
     ggCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGttt                                                                                                                                               <  2:1299613/148‑1 (MQ=255)
     ggCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGttt                                                                                                                                               <  2:3142704/148‑1 (MQ=255)
     ggCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGttt                                                                                                                                               <  2:1521317/148‑1 (MQ=255)
       cGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTAGTGttttt                                                                                                                                             <  2:1109841/148‑1 (MQ=255)
          aaTTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTcga                                                                                                                                          <  1:2830046/148‑1 (MQ=255)
          aaTTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTcga                                                                                                                                          >  2:2975621/1‑148 (MQ=255)
          aaTTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTAGTGTTTTTcga                                                                                                                                          <  1:1105081/148‑1 (MQ=255)
           aTTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTcgac                                                                                                                                         >  1:2288756/1‑148 (MQ=255)
              gttgCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTAGTGTTTTTCgacgac                                                                                                                                      <  2:61406/148‑1 (MQ=255)
                   aGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTaa                                                                                                                                 <  2:897029/148‑1 (MQ=255)
                   aGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTaa                                                                                                                                 <  2:3106045/148‑1 (MQ=255)
                   aGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGAATTATTTAGTGTTTTTCGACGACGGTaa                                                                                                                                 <  2:2596815/148‑1 (MQ=255)
                   aGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAACAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTaa                                                                                                                                 <  2:234970/148‑1 (MQ=255)
                    gACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAAt                                                                                                                                >  1:2801368/1‑148 (MQ=255)
                    gACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAAt                                                                                                                                >  2:2765019/1‑148 (MQ=255)
                       aGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCa                                                                                                                             <  2:2456605/148‑1 (MQ=255)
                        gTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCaa                                                                                                                            >  2:183785/1‑148 (MQ=255)
                        gTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTAGTGTTTTTCGACGACGGTAATTCaa                                                                                                                            >  2:354794/1‑148 (MQ=255)
                             gacatggacaGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTAGTGTTTTTCGACGACGGTAATTCAATGCgg                                                                                                                       >  1:2728940/1‑148 (MQ=255)
                               catggacaGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTCCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGaa                                                                                                                     <  2:1946173/148‑1 (MQ=255)
                                 tggacaGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGaaac                                                                                                                   >  1:1418862/1‑148 (MQ=255)
                                  ggacaGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGaaaca                                                                                                                  >  2:201790/1‑148 (MQ=255)
                                   gacaGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGaaacaa                                                                                                                 <  1:1870290/148‑1 (MQ=255)
                                   gacaGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGaaacaa                                                                                                                 <  1:3044389/148‑1 (MQ=255)
                                             agaAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGcc                                                                                                       >  2:1377862/1‑148 (MQ=255)
                                             agaAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGcc                                                                                                       >  1:2147843/1‑148 (MQ=255)
                                             agaAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGcc                                                                                                       >  1:1258965/1‑148 (MQ=255)
                                             agaAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGcc                                                                                                       >  1:3091842/1‑148 (MQ=255)
                                              gaAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGccc                                                                                                      >  1:2332202/1‑148 (MQ=255)
                                              gaAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGccc                                                                                                      >  1:2331964/1‑148 (MQ=255)
                                               aaaCCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCg                                                                                                     >  1:544698/1‑148 (MQ=255)
                                                 aCCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGct                                                                                                   >  1:35378/1‑148 (MQ=255)
                                                   cGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGctct                                                                                                 <  1:408658/148‑1 (MQ=255)
                                                    ggAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTAGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCtctc                                                                                                >  2:107610/1‑148 (MQ=255)
                                                     gAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCtcg                                                                                               <  2:933213/148‑1 (MQ=255)
                                                      aGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCtcgt                                                                                              <  2:2664074/148‑1 (MQ=255)
                                                        cGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCtcgtcg                                                                                            <  1:2844545/148‑1 (MQ=255)
                                                            cATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCa                                                                                        >  1:976469/1‑148 (MQ=255)
                                                             atatCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAg                                                                                       <  1:1663764/148‑1 (MQ=255)
                                                              tatCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGa                                                                                      >  1:942354/1‑148 (MQ=255)
                                                               atCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGAt                                                                                     <  1:825484/148‑1 (MQ=255)
                                                                 cGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTAGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATAc                                                                                   >  1:2437605/1‑148 (MQ=255)
                                                                  gTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACa                                                                                  >  1:2876627/1‑148 (MQ=255)
                                                                      gTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTc                                                                              <  2:162975/148‑1 (MQ=255)
                                                                         aaGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTcccc                                                                           <  2:1871164/148‑1 (MQ=255)
                                                                           gATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCtt                                                                         >  2:1646535/1‑148 (MQ=255)
                                                                            aTTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTg                                                                        >  1:590750/1‑148 (MQ=255)
                                                                             tttCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGc                                                                       <  2:2288771/148‑1 (MQ=255)
                                                                             tttCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGc                                                                       <  1:2306516/148‑1 (MQ=255)
                                                                              ttCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCa                                                                      >  2:79715/1‑148 (MQ=255)
                                                                                cGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATa                                                                    <  1:30026/148‑1 (MQ=255)
                                                                                  aGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACg                                                                  >  1:2854399/1‑148 (MQ=255)
                                                                                        gCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAAt                                                            >  2:79986/1‑148 (MQ=255)
                                                                                         cccGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATa                                                           >  2:2997483/1‑148 (MQ=255)
                                                                                             gggCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAAt                                                       >  1:1769120/1‑148 (MQ=255)
                                                                                             gggCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAAt                                                       <  2:3265842/148‑1 (MQ=255)
                                                                                             gggCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAAt                                                       <  2:1378481/148‑1 (MQ=255)
                                                                                               gCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATcc                                                     <  1:2104920/148‑1 (MQ=255)
                                                                                               gCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATcc                                                     <  2:1129570/148‑1 (MQ=255)
                                                                                                 cGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCt                                                   <  2:1521766/148‑1 (MQ=255)
                                                                                                 cGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCt                                                   <  1:2975621/148‑1 (MQ=255)
                                                                                                     cTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCa                                               >  1:2141769/1‑148 (MQ=255)
                                                                                                      tGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCaa                                              <  1:1382735/148‑1 (MQ=255)
                                                                                                       gACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAAt                                             <  1:183785/148‑1 (MQ=255)
                                                                                                        aCAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATg                                            >  1:1958444/1‑148 (MQ=255)
                                                                                                        aCAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTAGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATg                                            <  1:1354737/148‑1 (MQ=255)
                                                                                                             taaaataGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTaa                                       <  2:826130/148‑1 (MQ=255)
                                                                                                             taaaataGCCTGATAGGATAGGCTGTTAGCATTATTTAGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTaa                                       <  2:238729/148‑1 (MQ=255)
                                                                                                              aaaataGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAAt                                      >  1:3241016/1‑148 (MQ=255)
                                                                                                              aaaataGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAAt                                      >  2:477085/1‑148 (MQ=255)
                                                                                                                   aGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAg                                 >  2:666177/1‑148 (MQ=255)
                                                                                                                   aGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAg                                 >  1:2621001/1‑148 (MQ=255)
                                                                                                                       tGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCg                             >  1:2030313/1‑148 (MQ=255)
                                                                                                                       tGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATCCGGAAACAAACGTCTTCCCCGCTCTCGTCGACCAGATACAGTTCCCCTAGCATACGGCGAATACAATCCCTGGCACAGCTTAATCCCAGACCg                             >  1:3055440/1‑148 (MQ=255)
                                                                                                                          taggataggCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTg                          >  1:238980/1‑148 (MQ=255)
                                                                                                                           aggataggCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGc                         >  1:668036/1‑148 (MQ=255)
                                                                                                                             gataggCTGTTAGCATTATTTCGTGTTTTTCGACGCCGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGccc                       <  1:1155913/148‑1 (MQ=255)
                                                                                                                             gataggCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGccc                       >  1:737839/1‑148 (MQ=255)
                                                                                                                              ataggCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCt                      >  1:2806999/1‑148 (MQ=255)
                                                                                                                              ataggCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCt                      >  1:2809051/1‑148 (MQ=255)
                                                                                                                              ataggCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTGAATCCCAGACCGCTGCCCt                      <  2:2157353/148‑1 (MQ=255)
                                                                                                                              ataggCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTGAATCCCAGACCGCTGCCCt                      <  2:1237753/148‑1 (MQ=255)
                                                                                                                                  gCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCAc                  <  1:1388106/148‑1 (MQ=255)
                                                                                                                                  gCTGTTAGCATTATTTAGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCAc                  >  2:1452930/1‑148 (MQ=255)
                                                                                                                                     gTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGc               >  2:2212448/1‑148 (MQ=255)
                                                                                                                                      ttAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGcc              >  2:2449777/1‑148 (MQ=255)
                                                                                                                                        aGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGcccc            <  1:1377862/148‑1 (MQ=255)
                                                                                                                                        aGCATTATTTAGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGcccc            <  1:78092/148‑1 (MQ=255)
                                                                                                                                        aGCATTATTTAGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGcccc            <  1:2929405/148‑1 (MQ=255)
                                                                                                                                           attattTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCCttt         <  2:544698/148‑1 (MQ=255)
                                                                                                                                             tattTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCCTTTTc       >  1:2614603/1‑148 (MQ=255)
                                                                                                                                              attTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCCTTTTCg      <  1:2659803/148‑1 (MQ=255)
                                                                                                                                              attTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCCTTTTCg      <  1:1006189/148‑1 (MQ=255)
                                                                                                                                                ttCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCCTTTTCGCt    >  1:2174182/1‑148 (MQ=255)
                                                                                                                                                  cGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCCTTTTCGCtgg  >  2:1257598/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGTTCCCCTTGCATACGGCGAATACAATCCCTGGCAATGCTTAATCCCAGACCGCTGCCCTTCACCGCCCCTTTTCGCTGG  >  CP009273/2682890‑2683183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑