Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,695,522 C→A 13.1% E103* (GAG→TAG)  recO ← gap repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,695,5220CA13.1% 646.7 / 49.6 198E103* (GAG→TAG) recOgap repair protein
Reads supporting (aligned to +/- strand):  ref base C (88/84);  new base A (14/12);  total (102/96)
Fisher's exact test for biased strand distribution p-value = 8.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 211 total aligned reads displayed.

CACAATGGGTAAAATTGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGT  >  CP009273/2695375‑2695669
                                                                                                                                                   |                                                                                                                                                   
cacaATGGGTAAAATTGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTNGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGtctc                                                                                                                                                     <  1:1680092/148‑1 (MQ=255)
   aaTGGGTAAAATTGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTa                                                                                                                                                  <  2:2069623/148‑1 (MQ=255)
       ggTAAAATTGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTcc                                                                                                                                              <  2:591092/148‑1 (MQ=255)
        gTAAAATTGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCa                                                                                                                                             >  2:1283706/1‑148 (MQ=255)
           aaaTTGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTa                                                                                                                                          >  2:2275258/1‑148 (MQ=255)
               tGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTAcgcg                                                                                                                                      >  1:444815/1‑148 (MQ=255)
                   gCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGgag                                                                                                                                  <  1:2986027/148‑1 (MQ=255)
                      aTAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGgagaga                                                                                                                               >  1:1989728/1‑148 (MQ=255)
                      aTAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTAGTATTCCAGTACGCGGgagaga                                                                                                                               <  1:409604/148‑1 (MQ=255)
                         aCCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACTCGGGAGAGAAGt                                                                                                                            <  2:265397/148‑1 (MQ=255)
                             aGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTACTCGAAAAAAAGTTCAGAGAAGCGCGTCTAGTATTCCAGTACGCGGGAGAGAAGTTCGt                                                                                                                        >  2:54175/1‑148 (MQ=255)
                             aGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGt                                                                                                                        <  1:770786/148‑1 (MQ=255)
                               aTGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTg                                                                                                                      >  1:856685/1‑148 (MQ=255)
                               aTGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTg                                                                                                                      >  1:1850838/1‑148 (MQ=255)
                               aTGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTg                                                                                                                      <  2:48566/148‑1 (MQ=255)
                               aTGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTg                                                                                                                      <  1:2826618/148‑1 (MQ=255)
                               aTGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTg                                                                                                                      >  1:2728817/1‑148 (MQ=255)
                               aTGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTg                                                                                                                      <  2:1723315/148‑1 (MQ=255)
                                tGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGa                                                                                                                     <  2:1880368/148‑1 (MQ=255)
                                        cAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGa                                                                                                             <  1:2917323/148‑1 (MQ=255)
                                           tGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCg                                                                                                          >  1:1179850/1‑148 (MQ=255)
                                           tGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTAGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCg                                                                                                          >  1:2246779/1‑148 (MQ=255)
                                            gCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGc                                                                                                         <  2:1179850/148‑1 (MQ=255)
                                             ccAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCt                                                                                                        <  1:2855091/148‑1 (MQ=255)
                                                 ttCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTaa                                                                                                    >  2:1670464/1‑148 (MQ=255)
                                                   cAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAg                                                                                                  <  1:1025279/148‑1 (MQ=255)
                                                     aaGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGACTGCAGTGCAAGTACTCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCg                                                                                                <  2:1860025/148‑1 (MQ=255)
                                                     aaGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCg                                                                                                <  2:1615071/148‑1 (MQ=255)
                                                      aGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGt                                                                                               <  2:2258702/148‑1 (MQ=255)
                                                      aGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGt                                                                                               >  1:641389/1‑148 (MQ=255)
                                                      aGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGt                                                                                               <  2:2259772/148‑1 (MQ=255)
                                                       gcggcgCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTg                                                                                              <  1:2527449/148‑1 (MQ=255)
                                                       gcggcgCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTg                                                                                              <  2:85792/148‑1 (MQ=255)
                                                       gcggcgCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTAAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTg                                                                                              <  2:1759731/148‑1 (MQ=255)
                                                        cggcgCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTAGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGa                                                                                             <  2:1067758/148‑1 (MQ=255)
                                                          gcgcAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATa                                                                                           <  1:311079/148‑1 (MQ=255)
                                                             cAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCTTTGATGTACAGACCGCTGTAAAGCGTGATACCg                                                                                        >  1:349391/1‑148 (MQ=255)
                                                              aGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGc                                                                                       <  2:1160880/148‑1 (MQ=255)
                                                                cgcgGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGACGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCtt                                                                                     >  1:1798706/1‑148 (MQ=255)
                                                                      ttCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGc                                                                               >  2:1896221/1‑148 (MQ=255)
                                                                      ttCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGc                                                                               >  2:100511/1‑148 (MQ=255)
                                                                           gCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTAGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAgcgc                                                                          <  1:3294440/148‑1 (MQ=255)
                                                                            cGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGcc                                                                         <  1:2738424/148‑1 (MQ=255)
                                                                               aCCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTGGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGc                                                                      >  1:920687/1‑148 (MQ=255)
                                                                                ccAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCg                                                                     <  2:686610/148‑1 (MQ=255)
                                                                                      aCCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATCCCGCTTAATGGCAGCGCCAGCGAGACgg                                                               <  2:2095412/148‑1 (MQ=255)
                                                                                      aCCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACgg                                                               <  2:2095298/148‑1 (MQ=255)
                                                                                      aCCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTAGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATgg                                                                                <  1:1430385/131‑1 (MQ=255)
                                                                                      aCCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTAGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATgg                                                                                >  2:1939072/1‑131 (MQ=255)
                                                                                      aCCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTAGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATgg                                                                                >  2:1430385/1‑131 (MQ=255)
                                                                                       ccccTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGc                                                              >  2:3269349/1‑148 (MQ=255)
                                                                                        cccTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCt                                                             >  1:213747/1‑148 (MQ=255)
                                                                                         ccTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCtt                                                            <  2:236166/148‑1 (MQ=255)
                                                                                         ccTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCtt                                                            <  2:295520/148‑1 (MQ=255)
                                                                                         ccTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCtt                                                            <  2:2914554/148‑1 (MQ=255)
                                                                                           tGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCa                                                          >  1:2631028/1‑148 (MQ=255)
                                                                                            gCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTAGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAg                                                         <  2:927271/148‑1 (MQ=255)
                                                                                                gagaCTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACt                                                     <  1:390616/148‑1 (MQ=255)
                                                                                                gagaCTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACt                                                     >  1:3263065/1‑148 (MQ=255)
                                                                                                 agaCTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTg                                                    <  1:1631596/148‑1 (MQ=255)
                                                                                                 agaCTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTAGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTg                                                    <  2:1898731/148‑1 (MQ=255)
                                                                                                   aCTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTgcg                                                  >  2:3092495/1‑148 (MQ=255)
                                                                                                   aCTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTAGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTgcg                                                  >  2:1327095/1‑148 (MQ=255)
                                                                                                      gAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAg                                               >  2:299003/1‑148 (MQ=255)
                                                                                                      gAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTAGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAg                                               >  2:53188/1‑148 (MQ=255)
                                                                                                          gCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGtt                                           >  2:828747/1‑148 (MQ=255)
                                                                                                          gCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGtt                                           <  2:63775/148‑1 (MQ=255)
                                                                                                           cAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGttt                                          >  2:518541/1‑148 (MQ=255)
                                                                                                                cAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACt                                     >  2:1456143/1‑148 (MQ=255)
                                                                                                                  aGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTc                                   >  1:3168027/1‑148 (MQ=255)
                                                                                                                   gTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCg                                  >  2:3158693/1‑148 (MQ=255)
                                                                                                                    tAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGc                                 >  2:2295331/1‑148 (MQ=255)
                                                                                                                       tCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCAc                              >  1:707019/1‑148 (MQ=255)
                                                                                                                       tCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCAc                              >  1:707466/1‑148 (MQ=255)
                                                                                                                           aaaaaaGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCAcgccc                          >  2:1561548/1‑148 (MQ=255)
                                                                                                                           aaaaaaGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCAcgccc                          >  2:2372913/1‑148 (MQ=255)
                                                                                                                           aaaaaaGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCAcgccc                          <  1:236230/148‑1 (MQ=255)
                                                                                                                             aaaaGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCAcgcccgc                        >  1:1431767/1‑148 (MQ=255)
                                                                                                                              aaaGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCAcgcccgcc                       >  1:2404770/1‑148 (MQ=255)
                                                                                                                               aaGTTCAGAGAGGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCa                      >  1:1359801/1‑148 (MQ=255)
                                                                                                                               aaGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCa                      >  1:1275769/1‑148 (MQ=255)
                                                                                                                               aaGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTGTGACTTCGCCACGCCCGCCa                      >  2:2520113/1‑148 (MQ=255)
                                                                                                                                 gTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCaaa                    <  2:923113/148‑1 (MQ=255)
                                                                                                                                   tCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAAc                  >  2:2704968/1‑148 (MQ=255)
                                                                                                                                   tCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAAc                  >  1:1749665/1‑148 (MQ=255)
                                                                                                                                   tCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAAc                  >  1:164189/1‑148 (MQ=255)
                                                                                                                                         aaGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCa            >  1:2779530/1‑148 (MQ=255)
                                                                                                                                         aaGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTAAAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCa            >  1:2493996/1‑148 (MQ=255)
                                                                                                                                         aaGCGCGTCTAGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCa            <  2:1650969/148‑1 (MQ=255)
                                                                                                                                           gcgcGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAag          <  1:248459/148‑1 (MQ=255)
                                                                                                                                           gcgcGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAag          <  1:18916/148‑1 (MQ=255)
                                                                                                                                           gcgcGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAag          <  1:2275258/148‑1 (MQ=255)
                                                                                                                                            cgcgTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAaga         <  2:104917/148‑1 (MQ=255)
                                                                                                                                            cgcgTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAaga         <  1:1230238/148‑1 (MQ=255)
                                                                                                                                              cgTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAagaga       <  1:1380449/148‑1 (MQ=255)
                                                                                                                                                 ctcGTATTCCAGTACGCGGGAGAGAAGTTCTTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAggg    <  2:349391/148‑1 (MQ=255)
                                                                                                                                                 ctcGTATTCCAGTACGCGGGAGAGAAGTTCTTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAggg    <  2:2014210/148‑1 (MQ=255)
                                                                                                                                                 ctcGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAggg    >  1:2152843/1‑148 (MQ=255)
                                                                                                                                                  tcGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAgggg   <  1:1494269/148‑1 (MQ=255)
                                                                                                                                                  tcGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAgggg   <  1:2062534/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CACAATGGGTAAAATTGACGCCATAACCCAGATGCCCGAGCAGTGCCAGTTCAAAGCGGCGCAGCGCGGGTTCTGGCGTACCAGTGACCCCTGCAAGAGACTGAATGCAGTGCAAGTAATCGAAAAAAAGTTCAGAGAAGCGCGTCTCGTATTCCAGTACGCGGGAGAGAAGTTCGTTGATGTACAGACCGCTGTAAAGCGTGATACCGCTTAATGGCAGCGCCAGCGAGACGGCTTCAGCACTGCGCAGCGTTTTGACTTCGCCACGCCCGCCAAAACGTAGCAAGAGAGGGGT  >  CP009273/2695375‑2695669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑