Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,711,755 C→A 86.0% D45Y (GAC→TAC)  yfiF ← putative methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,711,7550CA86.0% 444.5 / 81.5 201D45Y (GAC→TAC) yfiFputative methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (17/11);  new base A (80/93);  total (97/104)
Fisher's exact test for biased strand distribution p-value = 2.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

Only 100 of 201 total aligned reads displayed.

TTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTC  >  CP009273/2711611‑2711896
                                                                                                                                                |                                                                                                                                             
ttttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGc                                                                                                                                            >  1:1685163/1‑148 (MQ=255)
   tACCAGTGTGGCCACCGTTTTCGGGCTTTGCGGGCTTCTCATCACCGGGGGCGCGGGAAAGCGTGGGCGACGGCGTATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCAACCGTAAGCACg                                                                                                                                         <  2:2186727/148‑1 (MQ=255)
      cacaGATCCCACCGTGATCGGCCTATTCCGGCGTCACATCACCCGGCGCGCGGGAAACCGTCCGCCACGGCGAATCATCCCACTAGCGGTCACCGGTCTGACTCTGTATGTCATCGCGGGCCGGGCGACGCCCACCGTAAGCACgaga                                                                                                                                      >  2:1053626/1‑148 (MQ=255)
      cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACgaga                                                                                                                                      >  2:2226647/1‑148 (MQ=255)
       aCTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGAt                                                                                                                                     >  2:620395/1‑148 (MQ=255)
       aCTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGAt                                                                                                                                     >  1:1435498/1‑148 (MQ=255)
       aCTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGAt                                                                                                                                     >  2:2278047/1‑148 (MQ=255)
       aCTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGAt                                                                                                                                     >  2:3175566/1‑148 (MQ=255)
        cTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGAtt                                                                                                                                    >  2:1712059/1‑148 (MQ=255)
        cTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGAtt                                                                                                                                    >  1:198637/1‑148 (MQ=255)
            ggCCACGGTGAGCGCCCTTTTCGGGCGTCTCAACCCCCGGCGCGCGGCAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTcc                                                                                                                                <  2:2667497/147‑1 (MQ=255)
             gCCACCGTGAGCGGCCTTTTCCGGCGTACCAACCCCGGGCGGGCGGGAACCCGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCt                                                                                                                               <  1:2760473/148‑1 (MQ=255)
             cccACCGTGTGGGGCGTTTGCCGGCTTCCCACCCCCGGGCGGGCGGTAAACCGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCt                                                                                                                               <  1:176802/147‑1 (MQ=255)
              ccccggTGCTCGCCCTTTGCGGGCGTCACACCCCCCGGCGGGCGGCACCCCGGCCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTg                                                                                                                              <  1:2552904/143‑1 (MQ=255)
              ccACCGTGCGCGGCCTTTCCCGGCGACACCCCCCCCGGCGCGCGCCACCCCGCGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTg                                                                                                                              <  1:1237826/148‑1 (MQ=255)
               cACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTgg                                                                                                                             >  2:1597739/1‑148 (MQ=255)
                aCCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCAGGa                                                                                                                            >  1:406666/1‑148 (MQ=255)
                 ccGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGAc                                                                                                                           >  2:2341278/1‑148 (MQ=255)
                    tGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcc                                                                                                                        >  2:623554/1‑148 (MQ=255)
                     gATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGAcgccc                                                                                                                       >  1:2579488/1‑148 (MQ=255)
                       tCGGCCTTTTCCGGAGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAcgcccgc                                                                                                                     >  2:547828/1‑148 (MQ=255)
                           ccTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGccccc                                                                                                                 >  2:319862/1‑148 (MQ=255)
                           ccTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGccccc                                                                                                                 >  2:1826918/1‑148 (MQ=255)
                           ccTTTTCCGGCGTCACACCACCCGGCGCGGGGCAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGccccc                                                                                                                 <  2:1288493/148‑1 (MQ=255)
                           ccTTTGCCGGGGACTAAGCACCCGGCGGGGGGTAAAGCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGccccc                                                                                                                 <  2:477607/148‑1 (MQ=255)
                               tgcgggggTCTCACCCCCGGGCGGGGGGTAACCGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTc                                                                                                             <  2:22731/141‑1 (MQ=255)
                               tgccGGCGTCACAGCCCCCGGCGCGCGGGAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTc                                                                                                             <  2:2947948/146‑1 (MQ=255)
                                       tctcACCCCCCGGCGGGGGGCAAACGGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAcg                                                                                                     <  2:288383/148‑1 (MQ=255)
                                        ctcATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAcgc                                                                                                    >  1:127207/1‑148 (MQ=255)
                                          catcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCgg                                                                                                  >  1:1084771/1‑148 (MQ=255)
                                           agcccccGGCGCGCGGCAAACCGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCggg                                                                                                 <  2:141088/144‑1 (MQ=255)
                                           aacccccGGCGCGGGGGAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCggg                                                                                                 <  1:798255/144‑1 (MQ=255)
                                            gcccccGGCGCGGGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGt                                                                                                <  2:2148686/145‑1 (MQ=255)
                                             caCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGtt                                                                                               >  1:617149/1‑148 (MQ=255)
                                              aCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtg                                                                                              >  2:3239730/1‑148 (MQ=255)
                                              aCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACGTGCGGTCACGGGGCTGACTCTGTTGGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtg                                                                                              >  2:1091252/1‑148 (MQ=255)
                                                 cGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTgg                                                                                           >  1:897739/1‑148 (MQ=255)
                                                  ggCGCGCGGTAACCGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTggg                                                                                          <  2:1032356/148‑1 (MQ=255)
                                                  ggCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTggg                                                                                          >  2:695445/1‑148 (MQ=255)
                                                   gcgcgcGGCAACCCGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGt                                                                                         <  1:3181978/148‑1 (MQ=255)
                                                     gcgcgGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTAc                                                                                       <  2:1025266/148‑1 (MQ=255)
                                                         gggAACCCGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGttt                                                                                   <  1:2818978/148‑1 (MQ=255)
                                                          ggAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTa                                                                                  >  2:1157374/1‑148 (MQ=255)
                                                           gAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTAt                                                                                 >  2:2721506/1‑148 (MQ=255)
                                                           gAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTAt                                                                                 <  2:800670/148‑1 (MQ=255)
                                                            aacccGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATc                                                                                <  2:3073919/145‑1 (MQ=255)
                                                              aCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAg                                                                              >  2:2748976/1‑148 (MQ=255)
                                                               ccGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGa                                                                             <  1:2943396/148‑1 (MQ=255)
                                                                     gcCACGGCGAATCTTCCCACTGGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAatcatc                                                                       <  2:2424915/148‑1 (MQ=255)
                                                                      ccACGGCGAATCGTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAatcatca                                                                      <  1:482251/148‑1 (MQ=255)
                                                                         cGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAAtcatcatca                                                                   <  1:1022341/148‑1 (MQ=255)
                                                                         cGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAAtcatcatca                                                                   <  1:2081873/148‑1 (MQ=255)
                                                                         cGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAAtcatcatca                                                                   <  1:2935188/148‑1 (MQ=255)
                                                                            cGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTg                                                                <  2:3253402/148‑1 (MQ=255)
                                                                              aaTCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCg                                                              >  2:2730044/1‑148 (MQ=255)
                                                                               aTCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCgg                                                             <  2:1245016/148‑1 (MQ=255)
                                                                               aTCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCgg                                                             >  2:235164/1‑148 (MQ=255)
                                                                               aTCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGCACGTTTATCAGAATCATCATCACTGCgg                                                             <  2:2746843/148‑1 (MQ=255)
                                                                                 cTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGac                                                           >  2:1420815/1‑148 (MQ=255)
                                                                                 cTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGac                                                           <  1:1121251/148‑1 (MQ=255)
                                                                                   tCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGacat                                                         <  1:1825470/148‑1 (MQ=255)
                                                                                    cccACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGacata                                                        <  2:2599587/148‑1 (MQ=255)
                                                                                     ccACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGacatac                                                       <  2:1555901/148‑1 (MQ=255)
                                                                                     ccACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGacatac                                                       <  2:1025292/148‑1 (MQ=255)
                                                                                      cACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAatcatca                                                                      <  1:3017013/132‑1 (MQ=255)
                                                                                      cACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGacataca                                                      >  2:1083853/1‑148 (MQ=255)
                                                                                        cTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATc                                                    >  2:2545273/1‑148 (MQ=255)
                                                                                         ttGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCa                                                   >  2:2522656/1‑148 (MQ=255)
                                                                                              gTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTg                                              <  1:2444264/148‑1 (MQ=255)
                                                                                               tCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGa                                             >  1:244136/1‑148 (MQ=255)
                                                                                                cACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGAc                                            <  2:2787855/148‑1 (MQ=255)
                                                                                                  cGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCt                                          >  2:2903316/1‑148 (MQ=255)
                                                                                                    gggCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTg                                        >  2:431145/1‑148 (MQ=255)
                                                                                                     ggCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGAAGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCAACACTGCGGACATACATCACTTTGAACTTga                                       >  2:248140/1‑147 (MQ=255)
                                                                                                         gACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCt                                   >  2:2278347/1‑148 (MQ=255)
                                                                                                         gACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCt                                   <  2:1432764/148‑1 (MQ=255)
                                                                                                          aCTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCtt                                  <  2:605411/148‑1 (MQ=255)
                                                                                                           ctctGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCttt                                 <  2:1223822/148‑1 (MQ=255)
                                                                                                           ctctGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCttt                                 <  2:2874244/148‑1 (MQ=255)
                                                                                                           ctctGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCgtt                                 >  1:1138237/1‑148 (MQ=255)
                                                                                                            tctGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCtttt                                <  2:1685163/148‑1 (MQ=255)
                                                                                                            tctGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCtttt                                >  2:115308/1‑148 (MQ=255)
                                                                                                                 ttGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCtt                           <  2:607580/148‑1 (MQ=255)
                                                                                                                   gTCATCGCGGGCGGGGCGAGGGCGTCCGTAAGCACGAGATTTTCCTGGACGCCCGCCGCCTTTCCCGGTACGCGTGTTGTGGGTAGGTTTATCAGAATCATCATCACTGCGGACATTCATGACTTTGACCTTGCCGCTTTTACCtttc                         <  1:1053626/148‑1 (MQ=255)
                                                                                                                     cATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCtttcat                       >  2:2036010/1‑148 (MQ=255)
                                                                                                                      aTCGCGGGCGGGGAGACGGCCACCGTAAGCACGAGATTTTCATGGACTCCAGCACCCTATCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTTCCtttgatt                      >  1:112928/1‑148 (MQ=255)
                                                                                                                       tCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCtttcattt                     >  2:2221419/1‑148 (MQ=255)
                                                                                                                        cgcgGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCtttcatttc                    <  1:235164/148‑1 (MQ=255)
                                                                                                                          cgggcgggGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTttcatttcat                  <  1:2721506/148‑1 (MQ=255)
                                                                                                                          cgggcgggGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTttcatttcat                  <  1:2725854/148‑1 (MQ=255)
                                                                                                                          cgggcgggGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATAATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTttcatttcat                  <  1:759546/148‑1 (MQ=255)
                                                                                                                            ggcggggcgACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCg                <  2:2390566/148‑1 (MQ=255)
                                                                                                                               ggggcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTc             <  1:3207908/148‑1 (MQ=255)
                                                                                                                                gggcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCa            <  1:1855450/148‑1 (MQ=255)
                                                                                                                                gggcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCa            <  1:3301579/148‑1 (MQ=255)
                                                                                                                                 ggcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCAt           >  2:499159/1‑148 (MQ=255)
                                                                                                                                 ggcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCAt           <  1:939480/148‑1 (MQ=255)
                                                                                                                                  gcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATg          <  1:1712059/148‑1 (MQ=255)
                                                                                                                                   cgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGc         <  2:1258824/148‑1 (MQ=255)
                                                                                                                                     aCGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCtt       >  1:2660711/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                             
TTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTC  >  CP009273/2711611‑2711896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑