Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,725,747 G→T 92.9% R596S (CGC→AGC)  clpB ← protein disaggregation chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,725,7470GT92.9% 463.6 / 28.3 169R596S (CGC→AGC) clpBprotein disaggregation chaperone
Reads supporting (aligned to +/- strand):  ref base G (5/7);  new base T (78/79);  total (83/86)
Fisher's exact test for biased strand distribution p-value = 7.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

Only 100 of 169 total aligned reads displayed.

GACACCGAGTGTTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGA  >  CP009273/2725602‑2725888
                                                                                                                                                 |                                                                                                                                             
gaCACCGAGTGTTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTAt                                                                                                                                             <  1:94899/148‑1 (MQ=255)
gaCACCGAGTGTTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTAt                                                                                                                                             <  1:94911/148‑1 (MQ=255)
 acacCGAGTGTTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTAtt                                                                                                                                            <  1:815533/148‑1 (MQ=255)
        gtgtTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATcc                                                                                                                                     >  2:1644182/1‑148 (MQ=255)
          gtTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATACAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGc                                                                                                                                   <  2:2976717/148‑1 (MQ=255)
          gtTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGc                                                                                                                                   <  2:2976726/148‑1 (MQ=255)
          gtTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGc                                                                                                                                   >  2:1466509/1‑148 (MQ=255)
            ttCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCa                                                                                                                                 >  1:2963767/1‑148 (MQ=255)
              ctcCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGc                                                                                                                               >  1:476010/1‑148 (MQ=255)
              ctcCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGc                                                                                                                               >  1:2140278/1‑148 (MQ=255)
                    aaaCTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCa                                                                                                                         <  1:1644182/148‑1 (MQ=255)
                       cTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACgg                                                                                                                      <  1:1697295/148‑1 (MQ=255)
                       cTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACgg                                                                                                                      <  1:2210818/148‑1 (MQ=255)
                        tCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGc                                                                                                                     >  1:2173163/1‑148 (MQ=255)
                           gACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTac                                                                                                                  >  2:1092250/1‑148 (MQ=255)
                             cATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTacga                                                                                                                <  1:543484/148‑1 (MQ=255)
                                atCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTAcgacga                                                                                                             >  2:3212004/1‑148 (MQ=255)
                                atCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTAcgacga                                                                                                             <  1:733078/148‑1 (MQ=255)
                                  cGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAAt                                                                                                           <  1:1645073/148‑1 (MQ=255)
                                  cGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAAt                                                                                                           <  2:2676573/148‑1 (MQ=255)
                                  cGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAAt                                                                                                           >  2:1327360/1‑148 (MQ=255)
                                      aCGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCg                                                                                                       <  2:603524/148‑1 (MQ=255)
                                       cGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGt                                                                                                      <  2:2698186/148‑1 (MQ=255)
                                        ggACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGtt                                                                                                     <  2:2101476/148‑1 (MQ=255)
                                          aCCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAg                                                                                                   <  1:1805110/148‑1 (MQ=255)
                                          aCCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAg                                                                                                   <  1:999936/148‑1 (MQ=255)
                                            cATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGAt                                                                                                 >  1:90039/1‑148 (MQ=255)
                                             aTCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATa                                                                                                <  1:1436900/148‑1 (MQ=255)
                                              tCGCCTCGTCGCTATCAAACATAAAGTTAGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATAc                                                                                               >  2:3128590/1‑148 (MQ=255)
                                               cGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATAcc                                                                                              >  1:2792852/1‑148 (MQ=255)
                                                gCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCg                                                                                             <  2:2380708/148‑1 (MQ=255)
                                                   tcgtcgCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCAt                                                                                          >  2:470110/1‑148 (MQ=255)
                                                   tcgtcgCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCAt                                                                                          >  2:1190713/1‑148 (MQ=255)
                                                     gtcgCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCa                                                                                        >  2:3065351/1‑148 (MQ=255)
                                                      tcgCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCaa                                                                                       >  2:2789858/1‑148 (MQ=255)
                                                         cTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCg                                                                                    >  1:158346/1‑148 (MQ=255)
                                                            tCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCtt                                                                                 <  2:77580/148‑1 (MQ=255)
                                                            tCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCtt                                                                                 <  2:77200/148‑1 (MQ=255)
                                                            tCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCtt                                                                                 >  2:766830/1‑148 (MQ=255)
                                                             cAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTc                                                                                >  1:1469410/1‑148 (MQ=255)
                                                               aaCATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAg                                                                                                                                >  1:1765369/1‑98 (MQ=255)
                                                               aaCATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAg                                                                                                                                <  2:1765369/98‑1 (MQ=255)
                                                               aaCATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGt                                                                              >  2:589576/1‑148 (MQ=255)
                                                               aaCATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGt                                                                              >  2:329350/1‑148 (MQ=255)
                                                               aaCATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGt                                                                              >  2:2077482/1‑148 (MQ=255)
                                                                aCATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGtt                                                                             >  2:2312875/1‑148 (MQ=255)
                                                                 cATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTc                                                                            >  2:1501395/1‑148 (MQ=255)
                                                                 cATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTc                                                                            <  2:789074/148‑1 (MQ=255)
                                                                  aTAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCt                                                                           <  2:2965327/148‑1 (MQ=255)
                                                                   tAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTg                                                                          >  1:2497999/1‑148 (MQ=255)
                                                                    acaggtCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGa                                                                         <  1:1749127/143‑1 (MQ=255)
                                                                    aaaGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACGAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGa                                                                         <  1:1749224/148‑1 (MQ=255)
                                                                    aaaGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGa                                                                         >  1:2549870/1‑148 (MQ=255)
                                                                    aaaGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGa                                                                         >  1:2550916/1‑148 (MQ=255)
                                                                    aaaGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGa                                                                         >  1:2864521/1‑148 (MQ=255)
                                                                     aaGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGAc                                                                        >  1:2842690/1‑148 (MQ=255)
                                                                      aGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGAcc                                                                       >  1:1903955/1‑148 (MQ=255)
                                                                      aGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCACCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGAcc                                                                       >  1:1683998/1‑148 (MQ=255)
                                                                      aGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCACCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGAcc                                                                       >  1:1684028/1‑148 (MQ=255)
                                                                        ttCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCaa                                                                     <  1:1853279/148‑1 (MQ=255)
                                                                           gCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTa                                                                  <  1:2611077/148‑1 (MQ=255)
                                                                            ccAGCGCCTTACAAAGCTCTTTTTACCCCACACCAGTTGGGCCGACGAACAGGAATGAACCAATCGGGCTATATGGATCCCCCAGCCCCGCACGGCTACGACGAATACCGTTAGAAACCGCATCAACCGCTGCGGTCTGACCAATAAc                                                                 >  2:246044/1‑148 (MQ=255)
                                                                             cAGCGCCTTACAAAGCTCTGTTTTCCCCCCACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTAcg                                                                <  2:285354/148‑1 (MQ=255)
                                                                               gcgcCTTACACAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGACCCTATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTAcgcg                                                              <  2:2971287/148‑1 (MQ=255)
                                                                                cgcCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGa                                                             <  2:2747353/148‑1 (MQ=255)
                                                                                cgcCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGa                                                             >  1:106922/1‑148 (MQ=255)
                                                                                   cTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATgg                                                          <  2:2963767/148‑1 (MQ=255)
                                                                                     tACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTg                                                        <  2:58991/148‑1 (MQ=255)
                                                                                      aCAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGc                                                       >  1:2270029/1‑148 (MQ=255)
                                                                                       cAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCa                                                      >  1:2114141/1‑148 (MQ=255)
                                                                                        aaaGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGACGGTGCAg                                                     <  2:2203178/148‑1 (MQ=255)
                                                                                        aaaGCTCTGTTGTCCCCACACCAGTTGGGCCGAGGCACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAg                                                     <  2:2489623/148‑1 (MQ=255)
                                                                                         gggCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGt                                                    >  1:1528335/3‑148 (MQ=255)
                                                                                           gCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTc                                                  <  1:2317940/148‑1 (MQ=255)
                                                                                             tctGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCtt                                                >  2:2207484/1‑148 (MQ=255)
                                                                                                 ttttCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTc                                            >  1:1964493/1‑148 (MQ=255)
                                                                                                 ttttCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTc                                            <  2:2125098/148‑1 (MQ=255)
                                                                                                 ttttCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTc                                            <  1:470110/148‑1 (MQ=255)
                                                                                                 ttttCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTc                                            >  1:776247/1‑148 (MQ=255)
                                                                                                       ccACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCACATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACg                                      >  2:199196/1‑148 (MQ=255)
                                                                                                            ccAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACgcagca                                 <  2:1207279/148‑1 (MQ=255)
                                                                                                             cAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGcagcag                                <  1:2182492/148‑1 (MQ=255)
                                                                                                                        aGGAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGcgct                     >  1:3227853/1‑148 (MQ=255)
                                                                                                                         ggAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGcgctc                    >  2:2444768/1‑148 (MQ=255)
                                                                                                                          gAACAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGcgctcg                   >  1:1065249/1‑148 (MQ=255)
                                                                                                                             cAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCtt                >  2:1834132/1‑148 (MQ=255)
                                                                                                                             cAGGAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCtt                >  2:1834083/1‑148 (MQ=255)
                                                                                                                               ggAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTc              >  1:3229107/1‑148 (MQ=255)
                                                                                                                                gAATGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTcc             >  1:2523445/1‑148 (MQ=255)
                                                                                                                                  aTGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCcat           >  2:269813/1‑148 (MQ=255)
                                                                                                                                  aTGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCcat           <  1:1092250/148‑1 (MQ=255)
                                                                                                                                  aTGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCcat           <  2:772843/148‑1 (MQ=255)
                                                                                                                                  aTGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCCCGCTTTCcat           >  2:1063926/1‑148 (MQ=255)
                                                                                                                                   tGAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCcatc          >  2:3201205/1‑148 (MQ=255)
                                                                                                                                    gAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCcatca         >  1:1331789/1‑148 (MQ=255)
                                                                                                                                    gAACCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCcatca         >  1:1331782/1‑148 (MQ=255)
                                                                                                                                     aaCCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCcatcat        >  1:638573/1‑148 (MQ=255)
                                                                                                                                      aCCAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATg       >  1:2664248/1‑148 (MQ=255)
                                                                                                                                        cAATCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCg     >  2:453/1‑148 (MQ=255)
                                                                                                                                           tCGGGCTATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCgaga  >  1:3269479/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
GACACCGAGTGTTTCTCCATAAACTCGGACATATCGATACGGACCATCGCCTCGTCGCTATCAAACATAAAGTTCGCCAGCGCCTTACAAAGCTCTGTTTTCCCCACACCAGTTGGGCCGAGGAACAGGAATGAACCAATCGGGCGATTTGGATCCGCCAGCCCCGCACGGCTACGACGAATAGCGTTAGATACCGCATCAACCGCTTCGTTCTGACCAATTACGCGATGGTGCAGTTCTTGCTCCATACGCAGCAGTTTTTCGCGCTCGCTTTCCATCATGCGAGA  >  CP009273/2725602‑2725888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑