Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 237,417 A→C 30.3% F792V (TTT→GTT)  fadE ← acyl coenzyme A dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273237,4170AC30.3% 190.6 / 205.0 205F792V (TTT→GTT) fadEacyl coenzyme A dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (74/68);  new base C (40/22);  total (114/91)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.25e-01

Only 100 of 212 total aligned reads displayed.

GGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGTTTTTACCCAGCTCTTTACA  >  CP009273/237270‑237564
                                                                                                                                                   |                                                                                                                                                   
 gAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAg                                                                                                                                                    >  1:2096082/1‑148 (MQ=255)
 gAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAg                                                                                                                                                    >  1:1585846/1‑148 (MQ=255)
  aaaaCCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGt                                                                                                                                                   <  2:827378/148‑1 (MQ=255)
    aaCCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGtca                                                                                                                                                 <  1:823864/148‑1 (MQ=255)
     aCCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGtcat                                                                                                                                                <  1:1965793/148‑1 (MQ=255)
      ccATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGtcatc                                                                                                                                               <  1:2959919/148‑1 (MQ=255)
           cGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGt                                                                                                                                          >  2:1333040/1‑148 (MQ=255)
           cGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGt                                                                                                                                          >  2:2583630/1‑148 (MQ=255)
               tGGCTTTAATTTTAAATAATTAGAGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACTCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTATACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCCACGTTAAt                                                                                                                                      >  2:384795/1‑148 (MQ=255)
                ggCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATa                                                                                                                                     >  2:2408406/1‑148 (MQ=255)
                ggCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATa                                                                                                                                     <  2:1377557/148‑1 (MQ=255)
                ggCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATa                                                                                                                                     >  2:1145176/1‑148 (MQ=255)
                  cTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACt                                                                                                                                   >  2:1875245/1‑148 (MQ=255)
                   tttAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTg                                                                                                                                  >  2:2198743/1‑148 (MQ=255)
                    ttAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGACTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTgc                                                                                                                                 >  2:1959690/1‑148 (MQ=255)
                    ttAATTTTAAATAATTAGCGGATAAAGAAACGGAGACGTTCGGCTCCGTTATTCAATTACGCGGCGTCAACTATACGCACTTTAACCCGCAACATAACCCCCGTCCTCGCCAGCACCTCCGGCTAAAAGTCATCAACCTTAAAACTga                                                                                                                                 >  1:28577/1‑147 (MQ=255)
                        ttttAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAg                                                                                                                             >  1:1833174/1‑148 (MQ=255)
                        ttttAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAg                                                                                                                             >  2:2808208/1‑148 (MQ=255)
                        ttttAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAg                                                                                                                             >  2:2232983/1‑148 (MQ=255)
                        ttttAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAg                                                                                                                             >  1:805768/1‑148 (MQ=255)
                        ttttAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAg                                                                                                                             >  1:2464208/1‑148 (MQ=255)
                        ttttAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAg                                                                                                                             <  1:1085713/148‑1 (MQ=255)
                            aaaTAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACgg                                                                                                                         <  1:3037815/148‑1 (MQ=255)
                            aaaTAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACgg                                                                                                                         <  1:2882724/148‑1 (MQ=255)
                            aaaTAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACgg                                                                                                                         <  1:2667297/148‑1 (MQ=255)
                             aataatTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGc                                                                                                                        <  2:2526943/148‑1 (MQ=255)
                             aataatTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGc                                                                                                                        <  1:1196981/148‑1 (MQ=255)
                              ataatTAGCGGATAAAGATACGGAGGATTTCGGCTCCGTTATTCATTTTCGAGGATTCAACTTTCCGCACTTTCTCCGGCTACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAATGTGATCAACGTTAATTCTGCGCAGACGGCt                                                                                                                       <  2:1844392/148‑1 (MQ=255)
                                 atTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTttc                                                                                                                    <  1:2682489/148‑1 (MQ=255)
                                       ggATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGc                                                                                                              <  2:647954/148‑1 (MQ=255)
                                       ggATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGc                                                                                                              <  1:2583630/148‑1 (MQ=255)
                                        gATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCt                                                                                                             >  1:1771647/1‑148 (MQ=255)
                                          tAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGATAATACTGCGCAGACGGCTTTCTTCAGCttt                                                                                                           >  1:1083407/1‑148 (MQ=255)
                                             aGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCAc                                                                                                        >  2:1258746/1‑148 (MQ=255)
                                             aGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCAc                                                                                                        <  2:413865/148‑1 (MQ=255)
                                             aGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCAc                                                                                                        >  2:2906700/1‑148 (MQ=255)
                                                aaCGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAg                                                                                                     >  2:36137/1‑148 (MQ=255)
                                                  cGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGaa                                                                                                   <  2:1410794/148‑1 (MQ=255)
                                                  cGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGACATCAACGTTAATACAGCGCAGACGGCATTCTTCAGCTTTCACCAGaa                                                                                                   >  2:1107406/1‑148 (MQ=255)
                                                   ggAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAAt                                                                                                  >  2:2553960/1‑148 (MQ=255)
                                                       ccTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCg                                                                                              <  2:1054279/148‑1 (MQ=255)
                                                        cTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCgg                                                                                             <  2:2050798/148‑1 (MQ=255)
                                                         tttCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGc                                                                                            >  2:703393/1‑148 (MQ=255)
                                                          ttCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCt                                                                                           >  1:329078/1‑148 (MQ=255)
                                                          ttCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCt                                                                                           <  2:602760/148‑1 (MQ=255)
                                                          ttCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCt                                                                                           >  1:1403234/1‑148 (MQ=255)
                                                          ttCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCt                                                                                           >  1:979311/1‑148 (MQ=255)
                                                               cTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATc                                                                                      <  2:864198/148‑1 (MQ=255)
                                                               cTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATc                                                                                      >  2:2111612/1‑148 (MQ=255)
                                                               cTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATc                                                                                      <  1:1603060/148‑1 (MQ=255)
                                                               cTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATc                                                                                      <  1:3278652/148‑1 (MQ=255)
                                                                 ccGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCtt                                                                                    >  1:2375516/1‑148 (MQ=255)
                                                                  cGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCttt                                                                                   >  1:627134/1‑148 (MQ=255)
                                                                  cGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCttt                                                                                   <  1:355975/148‑1 (MQ=255)
                                                                        tCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTatcaat                                                                             >  1:2541495/1‑148 (MQ=255)
                                                                               cgcgGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGcccc                                                                      >  2:1957574/1‑148 (MQ=255)
                                                                               cgcgGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGcccc                                                                      <  2:1200925/148‑1 (MQ=255)
                                                                                 cgGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCtt                                                                    <  1:68729/148‑1 (MQ=255)
                                                                                   gCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCa                                                                  >  2:1382424/1‑148 (MQ=255)
                                                                                     ttCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCAcc                                                                >  2:1507710/1‑148 (MQ=255)
                                                                                     ttCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTGTATCAATCAGCCCCTTCAcc                                                                >  2:3148510/1‑148 (MQ=255)
                                                                                       cAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAg                                                              >  1:2100394/1‑148 (MQ=255)
                                                                                       cAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAg                                                              >  1:3299806/1‑148 (MQ=255)
                                                                                         aCTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAgcg                                                            >  1:2023432/1‑148 (MQ=255)
                                                                                          cTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAgcgc                                                           >  2:217270/1‑148 (MQ=255)
                                                                                              ccGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTtg                                                       >  1:2182378/1‑148 (MQ=255)
                                                                                                      tctcCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAg                                               >  1:2252465/1‑148 (MQ=255)
                                                                                                       ctcCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGt                                              <  2:494410/148‑1 (MQ=255)
                                                                                                        tcCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGtt                                             <  2:1910334/148‑1 (MQ=255)
                                                                                                               aCTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAg                                      <  1:555371/148‑1 (MQ=255)
                                                                                                                cTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGa                                     <  2:376240/148‑1 (MQ=255)
                                                                                                                cTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGa                                     >  1:1511498/1‑148 (MQ=255)
                                                                                                                   tACCGGCTTCGTCGCCAGCTCTTCCGGATCATAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACgg                                  <  2:3020948/148‑1 (MQ=255)
                                                                                                                   tACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACgg                                  <  2:2284681/148‑1 (MQ=255)
                                                                                                                   tACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACgg                                  <  2:2635035/148‑1 (MQ=255)
                                                                                                                       ggCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTaa                              <  2:784827/148‑1 (MQ=255)
                                                                                                                          ttCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACg                           >  2:3009066/1‑148 (MQ=255)
                                                                                                                              tcgCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAg                       <  1:2493122/148‑1 (MQ=255)
                                                                                                                               cgCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAgg                      >  2:477693/1‑148 (MQ=255)
                                                                                                                                gCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGt                     <  2:934107/148‑1 (MQ=255)
                                                                                                                                 ccAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGCCGGCTTTCTTCAGCTTTCACCAGAATAGGGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGtt                    <  1:1291332/148‑1 (MQ=255)
                                                                                                                                  cAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGttt                   <  1:188246/148‑1 (MQ=255)
                                                                                                                                   aGCTCTTCCGGATCAAAGTCATCAACTTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGtttt                  <  1:1152242/148‑1 (MQ=255)
                                                                                                                                   aGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGtttt                  <  1:1052932/148‑1 (MQ=255)
                                                                                                                                   aGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGtttt                  <  1:150527/148‑1 (MQ=255)
                                                                                                                                    gCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTGTCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGttttt                 <  2:1187261/148‑1 (MQ=255)
                                                                                                                                     ctcaTCCGGATAAAAGTCATCAACGATAATACAGCTCAGACGGCTATATTCAGATTTCACCAGAATAGCGGCTTCATCTTTATAAATCAGCACCTTCAAAATCTCTTTGTGCGCCAATTCATAAAGAAGGGTAAAAGTCACGttatta                >  2:2698379/1‑148 (MQ=255)
                                                                                                                                      tctTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGTTTTTAc               >  2:635352/1‑148 (MQ=255)
                                                                                                                                        ttCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGTTTTTAccc             >  2:3284739/1‑148 (MQ=255)
                                                                                                                                            ggATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGTTTTTACCCAGct         >  1:1860689/1‑148 (MQ=255)
                                                                                                                                            ggATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCCGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGTTTTTACCCAGct         <  1:2554203/148‑1 (MQ=255)
                                                                                                                                             gATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGTTTTTACCCAGctc        >  1:1022888/1‑148 (MQ=255)
                                                                                                                                                cAAAGTCATCACCGTTAATACTGCGCCGCCGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGTTTTTACCCAGCTCttt     <  1:1333040/148‑1 (MQ=255)
                                                                                                                                                cAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGTTTTTACCCAGCTCttt     >  1:1915415/1‑148 (MQ=255)
                                                                                                                                                cAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGTTTTTACCCAGCTCttt     <  1:2570910/148‑1 (MQ=255)
                                                                                                                                                cAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGTTTTTACCCAGCTCttt     >  2:72285/1‑148 (MQ=255)
                                                                                                                                                cAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGTTTTTACCCAGCTCttt     >  2:1747852/1‑148 (MQ=255)
                                                                                                                                                 aaaGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGTTTTTACCCAGCTCTTTa    <  1:401859/148‑1 (MQ=255)
                                                                                                                                                  aaGTCATCAACGTTACTACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCTTTGTGCGACAGTTCATCCAGACGGGTAAACGGCAGGTTTTTACCCAGCTCTTTAc   <  2:28577/148‑1 (MQ=255)
                                                                                                                                                   aGTCAGCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGGGCGCCAGTTCATCCAGACGGGTAAACGGCAGGTTTTTACCCAGCTCTTTACa  <  1:1959652/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGTTTTTACCCAGCTCTTTACA  >  CP009273/237270‑237564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑