Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 31,710 C→A 100% I298I (ATC→ATA)  carB → carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927331,7100CA98.4% 595.2 / ‑0.3 197I298I (ATC→ATA) carBcarbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (115/77);  total (117/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 198 total aligned reads displayed.

TCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCC  >  CP009273/31565‑31854
                                                                                                                                                 |                                                                                                                                                 
tCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGa                                                                                                                                                 >  1:1528728/1‑148 (MQ=255)
tCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGAGTGTTATAGa                                                                                                                                                 >  2:105231/1‑148 (MQ=255)
 cgcgCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGaa                                                                                                                                                <  1:544163/148‑1 (MQ=255)
  gcgcCAGCCCAACCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGaaa                                                                                                                                               <  2:1611668/148‑1 (MQ=255)
     ccATCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGa                                                                                                                                            <  1:1008378/148‑1 (MQ=255)
     ccAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGa                                                                                                                                            <  2:965512/148‑1 (MQ=255)
     ccAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGa                                                                                                                                            <  2:3061269/148‑1 (MQ=255)
         cccAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAAccc                                                                                                                                        >  1:2744256/1‑148 (MQ=255)
         cccAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAAccc                                                                                                                                        >  2:324444/1‑148 (MQ=255)
          ccAACCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCa                                                                                                                                       <  2:688284/148‑1 (MQ=255)
           cAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAc                                                                                                                                      >  2:716871/1‑148 (MQ=255)
            aaaCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAcg                                                                                                                                     >  2:2920725/1‑148 (MQ=255)
            aaaCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAcg                                                                                                                                     <  2:2586368/148‑1 (MQ=255)
            aaaCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAcg                                                                                                                                     >  2:3557342/1‑148 (MQ=255)
            aaaCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAcg                                                                                                                                     >  1:3394551/1‑148 (MQ=255)
              aCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAcgcg                                                                                                                                   >  1:3248208/1‑148 (MQ=255)
              aCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAcgcg                                                                                                                                   >  1:3160946/1‑148 (MQ=255)
                gCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCgt‑g                                                                                                                                <  1:1493950/148‑1 (MQ=255)
                 cTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCgt‑gt                                                                                                                               >  1:540898/1‑148 (MQ=255)
                   gACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTcc                                                                                                                             <  2:117495/148‑1 (MQ=255)
                      cGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGt                                                                                                                          <  1:1921518/148‑1 (MQ=255)
                        aCAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGttc                                                                                                                        >  2:1789931/1‑148 (MQ=255)
                        aCAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGttc                                                                                                                        >  1:1991859/1‑148 (MQ=255)
                         cAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGttct                                                                                                                       <  1:3580415/148‑1 (MQ=255)
                          aaaGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGttctt                                                                                                                      <  1:138755/148‑1 (MQ=255)
                           aaGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGttcttc                                                                                                                     >  1:2993697/1‑148 (MQ=255)
                           aaGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGttcttc                                                                                                                     <  2:537626/148‑1 (MQ=255)
                              aaTATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGgc                                                                                                                  >  2:2321179/1‑148 (MQ=255)
                                tatCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGgcgc                                                                                                                <  1:1280332/148‑1 (MQ=255)
                                tatCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGgcgc                                                                                                                >  2:511252/1‑148 (MQ=255)
                                      aTCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGt                                                                                                          >  1:2850766/1‑148 (MQ=255)
                                      aTCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGt                                                                                                          <  2:2537958/148‑1 (MQ=255)
                                      aTCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGt                                                                                                          >  2:2194402/1‑148 (MQ=255)
                                        cATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCg                                                                                                        >  1:3320783/1‑148 (MQ=255)
                                        cATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAAAGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCg                                                                                                        >  1:1418377/1‑148 (MQ=255)
                                           gCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGTTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGaaa                                                                                                     <  2:418336/148‑1 (MQ=255)
                                           gCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGaaa                                                                                                     >  2:1291197/1‑148 (MQ=255)
                                           gCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGaaa                                                                                                     >  2:2785854/1‑148 (MQ=255)
                                              tAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCg                                                                                                  <  1:1951868/148‑1 (MQ=255)
                                              tAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCg                                                                                                  >  1:2628784/1‑148 (MQ=255)
                                               aaCGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGa                                                                                                 >  1:3648873/1‑148 (MQ=255)
                                               aaCGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGa                                                                                                 >  1:3647570/1‑148 (MQ=255)
                                                 cGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAcc                                                                                               >  1:2224569/1‑148 (MQ=255)
                                                   ccTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCgg                                                                                             >  1:1619631/1‑148 (MQ=255)
                                                     tCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGtt                                                                                           >  2:803039/1‑148 (MQ=255)
                                                     tCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGtt                                                                                           >  1:923153/1‑148 (MQ=255)
                                                           gCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGa                                                                                     >  1:3114536/1‑148 (MQ=255)
                                                            cGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCACCGTTCAGTTTGTGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCGGGCGTCTAAAGCGACCGGTTTCCCGAt                                                                                    <  2:2788713/148‑1 (MQ=255)
                                                                   gcGTGAAATCGGCTTTGAAACGGGTGGTTCAAACGTTCTGTTTGGGGTGAACCCGAAAAACGGTCGTCTGATTTTTATAGAAATGAACCCCCGCGT‑GGCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTgaa                                                                             <  2:857514/148‑1 (MQ=255)
                                                                   gcGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTaaa                                                                             >  2:1639386/1‑148 (MQ=255)
                                                                    cttGAAATCGGCGTTTAAACGGGGGGTTCCAAGGTTCAGTTTGGGGTCACCCCCAAAACGGGTCGTGTTTTTGTTTTAGAAATGAACCCCCGCGG‑GGCCGGTTCTTCGGCGCTGGCGTCTAAAGCGACCGGTTTCCCGATTGCTAAAg                                                                            <  2:3569700/146‑1 (MQ=255)
                                                                     gTGAAAGCGGCGTTGAAACGGGGGGTTCCACCGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTGTGATTGTTATATAAATGACCCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGt                                                                           <  2:3406916/148‑1 (MQ=255)
                                                                       taaaTCGGCGTTGAACCGGGGGGTTCCAAGGTTCTGTTTGCGGTGAACCCGAAAACCGGTCGTCTGATTGTTATAGAAATGAACCCCCGGGG‑GGCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTgg                                                                         <  2:171296/147‑1 (MQ=255)
                                                                       gAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGACCCCACGCGT‑GGCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTgg                                                                         <  2:3162188/148‑1 (MQ=255)
                                                                         aaTCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTggcg                                                                       >  1:2096567/1‑148 (MQ=255)
                                                                          aTCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTggcgg                                                                      >  1:3601551/1‑148 (MQ=255)
                                                                          aTCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAAACAACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTggcgg                                                                      >  1:1707513/1‑148 (MQ=255)
                                                                            cGGCGTTGAACCGGGTGGTTCCAAGGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGGGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGgcggcg                                                                    <  2:3281255/148‑1 (MQ=255)
                                                                               ctttGAACCCGGTGGTTCAAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGACTGATTGTTATAGAAATGACCCCACGCGT‑GTCCCGTTCTTCGGCGCGGGCTTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGaaa                                                                 <  2:2850766/146‑1 (MQ=255)
                                                                               cGTTGAACCGGGTGGTTCCAACTTTCTGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTTTTATAGAAATGAACCCACGCGG‑GGCCCGTTCTTCGGCGCGGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGaaa                                                                 <  2:2682829/148‑1 (MQ=255)
                                                                                 ttGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACt                                                               >  1:1811358/1‑148 (MQ=255)
                                                                                  tGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTGATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTg                                                              >  2:2215213/1‑148 (MQ=255)
                                                                                          tgggtTCCAACGTTCAGTTTGCGTTGAACCCTAAAAAGGGTCGTCTGATTGTTAAATAACGGAACCCCCGGGT‑GTCCCGTTCTGCGGCGGTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTggg                                                      <  2:3731569/146‑1 (MQ=255)
                                                                                                ccACCGTTCAGTTTGCGGCCAACCCCAAAAACGGTGGTCTGTTTTTTATACAAATGACCCCACGGGG‑GCCCGGTTCTTCGGCGCGGGCGTCGAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTacac                                                <  1:1874600/148‑1 (MQ=255)
                                                                                                 cAACGTTCTGTTGGCGGTCAACCCCAAAAACGGTCGTCTGATTTTTATAGAAATCACCCCCGGGGG‑GGCCCGTTCTGCGGCGCGGGCGCCCAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACAcc                                               <  1:2529372/148‑1 (MQ=255)
                                                                                                  aaCGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACAccc                                              >  1:1771028/1‑148 (MQ=255)
                                                                                                   aCGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGACCCCACGCGG‑GGCCCGTTCTTCGGCGCGGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCt                                             <  2:2032738/148‑1 (MQ=255)
                                                                                                    cGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTc                                            >  2:763109/1‑148 (MQ=255)
                                                                                                      ttCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcga                                          >  2:3361799/1‑148 (MQ=255)
                                                                                                      ttCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcga                                          >  1:1533504/1‑148 (MQ=255)
                                                                                                       tCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcgac                                         >  1:419221/1‑148 (MQ=255)
                                                                                                       tCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGGCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcgac                                         >  1:781184/1‑148 (MQ=255)
                                                                                                        cAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcgacg                                        >  2:3123888/1‑148 (MQ=255)
                                                                                                         tgTTGGCGGTCACCCCAAAAACCGGTGGTCTTATTTTTATATAAATCACCCCCCGCGG‑GGCCCGTTCTTGGGCGCTGGCGTCCAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcgacga                                       <  1:1028808/147‑1 (MQ=255)
                                                                                                           tttGCGGTTAACCCTAAAAAGGGTGGTTTTTTTGTTATATAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAAc                                     <  2:3594144/148‑1 (MQ=255)
                                                                                                            ttGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACt                                    >  1:2578000/1‑148 (MQ=255)
                                                                                                               ggttcaaCCCTAAAAAGGGTCGTCTTTTTTTTATAGAAATCACCCCCCGGGT‑GCCCGGTTCTGCGGCGCTGGCGTCCAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACtgat                                 <  1:406760/143‑1 (MQ=255)
                                                                                                               gggTCAACCCCAAAAAGGGTGGTCTTATTGTTATAAAAATCACCCCCCGCGG‑GGCCCGTTCTGCGGCGGGGGCGACCAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACtgat                                 <  1:2356296/147‑1 (MQ=255)
                                                                                                                    aaCCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACg                            >  2:1476822/1‑148 (MQ=255)
                                                                                                                     aCCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGa                           >  1:674425/1‑148 (MQ=255)
                                                                                                                     aCCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGa                           >  1:736560/1‑148 (MQ=255)
                                                                                                                      ccccAAAACCGGTCGTCTGATTGTTATAGAAAAGAACCCCCGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGAc                          <  2:3647570/148‑1 (MQ=255)
                                                                                                                      cccTAAAATGGGTGGTCTTATTTTTTTTGAATTG‑ACCCCCGGGTGGTCCCGTTCGTGGGCGTTGGCGTCGAACGCGAGCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGAc                          <  2:3648873/148‑1 (MQ=255)
                                                                                                                      cccTAAAAAGGGTCGTCTGATTTTTATACAAATCACCCCACGCGG‑GTCCCGTTCTTCGGCGCGGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGAc                          <  1:3774717/148‑1 (MQ=255)
                                                                                                                      cccGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGAc                          >  2:1348051/1‑148 (MQ=255)
                                                                                                                            aaaCGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACt                    >  1:123749/1‑148 (MQ=255)
                                                                                                                             aaCGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTg                   >  2:3144527/1‑148 (MQ=255)
                                                                                                                             aaCGGTCGTCTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTg                   >  1:3220324/1‑148 (MQ=255)
                                                                                                                                 gtcgtcTGATTGTTATAGAATTGAACCCCCGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCgg               <  2:2697280/148‑1 (MQ=255)
                                                                                                                                   cgtcTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGAc             >  2:1567051/1‑148 (MQ=255)
                                                                                                                                      cTGATTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCTCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTa          >  2:2600806/1‑148 (MQ=255)
                                                                                                                                         aTTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTc       >  2:1218917/1‑148 (MQ=255)
                                                                                                                                         aTTGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTc       >  2:1189661/1‑148 (MQ=255)
                                                                                                                                          ttGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTcc      >  1:3612023/1‑148 (MQ=255)
                                                                                                                                          ttGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTcc      >  2:1075440/1‑148 (MQ=255)
                                                                                                                                           tGTTATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCg     >  1:2029049/1‑148 (MQ=255)
                                                                                                                                           tGTTATAGAAATGAACCCACGCGT‑GTCACGTTCTTCGGCGCTGGCGTCGAAAGCGACAGGATTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGATACACACTCGACTAACTGAAGAACTACATCACTGCCGGAAGTACTCCg     >  1:3278282/1‑148 (MQ=255)
                                                                                                                                             ttATCGAATTGAACCCACGCGT‑GTCCGGTGCTTCGGGGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAATTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGc   <  2:2868385/148‑1 (MQ=255)
                                                                                                                                             ttATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGc   >  2:385485/1‑148 (MQ=255)
                                                                                                                                             ttATAGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGc   >  2:3445067/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TCGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGT‑GTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCC  >  CP009273/31565‑31854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑