Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 282,483 C→A 7.6% H460N (CAT→AAT)  yagG → CP4‑6 prophage; putative sugar transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273282,4830CA 7.6% 606.9 / 17.4 165H460N (CAT→AAT) yagGCP4‑6 prophage; putative sugar transporter
Reads supporting (aligned to +/- strand):  ref base C (77/75);  new base A (4/9);  total (81/84)
Fisher's exact test for biased strand distribution p-value = 2.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 171 total aligned reads displayed.

GTGCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTACCACTCCCGTG  >  CP009273/282336‑282629
                                                                                                                                                   |                                                                                                                                                  
gtgCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATccc                                                                                                                                                    <  2:1572356/148‑1 (MQ=255)
 tgCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCa                                                                                                                                                   <  1:3168526/148‑1 (MQ=255)
 tgCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCa                                                                                                                                                   >  2:3513083/1‑148 (MQ=255)
 tgCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCa                                                                                                                                                   >  2:2094057/1‑148 (MQ=255)
         gtgCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCgg                                                                                                                                           <  2:91536/148‑1 (MQ=255)
                aCGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAat                                                                                                                                    <  2:3549737/148‑1 (MQ=255)
                aCGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAat                                                                                                                                    <  1:3810906/148‑1 (MQ=255)
                aCGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAat                                                                                                                                    >  1:162540/1‑148 (MQ=255)
                 cgcgGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAata                                                                                                                                   <  1:2779189/148‑1 (MQ=255)
                  gcgGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAatat                                                                                                                                  <  2:1926247/148‑1 (MQ=255)
                      gCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCACTATGGaa                                                                                                                              >  1:3607547/1‑148 (MQ=255)
                      gCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGaa                                                                                                                              >  2:2309514/1‑148 (MQ=255)
                      gCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGaa                                                                                                                              <  1:1304180/148‑1 (MQ=255)
                      gCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGaa                                                                                                                              <  1:3699524/148‑1 (MQ=255)
                      gCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGaa                                                                                                                              <  1:3699522/148‑1 (MQ=255)
                         tGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATc                                                                                                                           <  1:2022688/148‑1 (MQ=255)
                          gTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCa                                                                                                                          <  2:198015/148‑1 (MQ=255)
                          gTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCa                                                                                                                          >  2:3559410/1‑148 (MQ=255)
                          gTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCa                                                                                                                          <  1:1084635/148‑1 (MQ=255)
                             catcatGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCAATTAACCGGAGGCAATATGGAAATCACTa                                                                                                                       <  2:2578621/148‑1 (MQ=255)
                              atcatGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTaa                                                                                                                      <  1:1135932/148‑1 (MQ=255)
                                catGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCAATTAACCGGAGGCAATATGGAAATCACTAAcc                                                                                                                    >  2:416163/1‑148 (MQ=255)
                                 atGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAAccc                                                                                                                   >  2:2030364/1‑148 (MQ=255)
                                 atGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAAccc                                                                                                                   >  1:350452/1‑148 (MQ=255)
                                  tGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCg                                                                                                                  <  1:2278807/148‑1 (MQ=255)
                                   gCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGa                                                                                                                 <  2:2435738/148‑1 (MQ=255)
                                   gCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGa                                                                                                                 >  2:1205541/1‑148 (MQ=255)
                                    cTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGAt                                                                                                                <  1:3783099/148‑1 (MQ=255)
                                     tGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATa                                                                                                               <  1:1015308/148‑1 (MQ=255)
                                      gTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATAc                                                                                                              >  2:2681824/1‑148 (MQ=255)
                                      gTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATAc                                                                                                              >  2:2239088/1‑148 (MQ=255)
                                             tACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCgg                                                                                                       <  2:3815100/148‑1 (MQ=255)
                                              aCAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGc                                                                                                      >  1:557426/1‑148 (MQ=255)
                                               cAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCACTATGGAAATCACTAACCCGATACTCACCGGCt                                                                                                     >  2:1804561/1‑148 (MQ=255)
                                               cAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCt                                                                                                     <  2:344724/148‑1 (MQ=255)
                                                   cTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCaa                                                                                                 >  2:523836/1‑148 (MQ=255)
                                                   cTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCAATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCaa                                                                                                 <  2:431588/148‑1 (MQ=255)
                                                      aCCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAAccc                                                                                              >  1:2488633/1‑148 (MQ=255)
                                                       ccGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAACTCACTAACCCGATACTCACCGGCTTCAACCCg                                                                                             >  1:1982701/1‑148 (MQ=255)
                                                          aTGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGAc                                                                                          >  1:3151388/1‑148 (MQ=255)
                                                              ccGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGt                                                                                      <  2:2805161/148‑1 (MQ=255)
                                                               cgcgTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTc                                                                                     >  2:801940/1‑148 (MQ=255)
                                                               cgcgTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTc                                                                                     <  1:3345689/148‑1 (MQ=255)
                                                                     gAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCtgtg                                                                               >  2:3579301/1‑148 (MQ=255)
                                                                       ggCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTgcc                                                                             >  1:454795/1‑148 (MQ=255)
                                                                         ccATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTgccgc                                                                           <  1:1999953/148‑1 (MQ=255)
                                                                          cATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTgccgcc                                                                          <  2:45224/148‑1 (MQ=255)
                                                                           aTCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCa                                                                         <  2:3659125/148‑1 (MQ=255)
                                                                           aTCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCa                                                                         <  2:3659322/148‑1 (MQ=255)
                                                                           aTCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCa                                                                         >  1:3310215/1‑148 (MQ=255)
                                                                            tCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAg                                                                        >  2:864567/1‑148 (MQ=255)
                                                                            tCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAg                                                                        <  1:210883/148‑1 (MQ=255)
                                                                             cAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAgg                                                                       >  2:1930282/1‑148 (MQ=255)
                                                                              aGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCCATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAggg                                                                      >  1:2193505/1‑148 (MQ=255)
                                                                              aGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAggg                                                                      >  1:2193519/1‑148 (MQ=255)
                                                                               gCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGc                                                                     >  2:1106630/1‑148 (MQ=255)
                                                                               gCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGc                                                                     >  2:1106623/1‑148 (MQ=255)
                                                                                ccGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCg                                                                    >  1:2984551/1‑148 (MQ=255)
                                                                                   gCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAgg                                                                 >  1:1439307/1‑148 (MQ=255)
                                                                                    cAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGa                                                                <  1:835451/148‑1 (MQ=255)
                                                                                     aGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGac                                                               <  2:2868697/148‑1 (MQ=255)
                                                                                     aGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGac                                                               <  2:2868635/148‑1 (MQ=255)
                                                                                        tGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGactac                                                            <  2:1659854/148‑1 (MQ=255)
                                                                                           ttAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACtactac                                                         <  1:569412/148‑1 (MQ=255)
                                                                                           ttAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACtactac                                                         <  1:569413/148‑1 (MQ=255)
                                                                                               gCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCg                                                     >  1:298123/1‑148 (MQ=255)
                                                                                               gCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCg                                                     >  1:1282725/1‑148 (MQ=255)
                                                                                               gCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCg                                                     >  2:2980321/1‑148 (MQ=255)
                                                                                               gCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAAGATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCg                                                     >  2:2980242/1‑148 (MQ=255)
                                                                                                cACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGc                                                    >  2:2863416/1‑148 (MQ=255)
                                                                                                  ccGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCa                                                  >  2:3341137/1‑148 (MQ=255)
                                                                                                       cggcgCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCg                                             >  2:692587/1‑148 (MQ=255)
                                                                                                          cgcAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGAcc                                          <  2:816411/148‑1 (MQ=255)
                                                                                                          cgcAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGAcc                                          <  2:3260833/148‑1 (MQ=255)
                                                                                                           gcAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCt                                         <  1:1736075/148‑1 (MQ=255)
                                                                                                              gggCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTCCTACATCGCCACCTCGACCTTCg                                      >  2:1429733/1‑148 (MQ=255)
                                                                                                              gggCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCg                                      >  2:2347662/1‑148 (MQ=255)
                                                                                                                 cGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGt                                   <  1:3601915/148‑1 (MQ=255)
                                                                                                                 cGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGt                                   <  1:70069/148‑1 (MQ=255)
                                                                                                                    ggCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGt                                >  2:1992344/1‑148 (MQ=255)
                                                                                                                       cGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTcc                             <  2:557426/148‑1 (MQ=255)
                                                                                                                          tCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCgg                          >  2:3399249/1‑148 (MQ=255)
                                                                                                                             cGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCg                       >  2:2719788/1‑148 (MQ=255)
                                                                                                                             cGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCg                       <  1:1578514/148‑1 (MQ=255)
                                                                                                                             cGACGCCGCGACAGCCGCATCCAATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCg                       <  1:1833339/148‑1 (MQ=255)
                                                                                                                                  ccgcGACAGCCGCATCCAATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTgcgc                  <  1:416163/148‑1 (MQ=255)
                                                                                                                                  ccgcGACAGCCGCATCCAATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTgcgc                  <  1:2340088/148‑1 (MQ=255)
                                                                                                                                    gcgACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCAt                <  2:350452/148‑1 (MQ=255)
                                                                                                                                      gACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCt              >  1:1825801/1‑148 (MQ=255)
                                                                                                                                       aCAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGAGACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTa             <  1:3513083/148‑1 (MQ=255)
                                                                                                                                        cAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTAc            >  1:2460789/1‑148 (MQ=255)
                                                                                                                                          gCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTACCa          <  1:1528444/148‑1 (MQ=255)
                                                                                                                                          gCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTACCa          <  2:2450554/148‑1 (MQ=255)
                                                                                                                                             gCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTACCACTc       <  2:1402227/148‑1 (MQ=255)
                                                                                                                                             gCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTACCACTc       <  1:3688485/148‑1 (MQ=255)
                                                                                                                                             gCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTACCACTc       <  2:634424/148‑1 (MQ=255)
                                                                                                                                             gCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTACCACTc       >  1:349087/1‑148 (MQ=255)
                                                                                                                                                tCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTACCACTCCCg    <  1:1889992/148‑1 (MQ=255)
                                                                                                                                                tCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTACCACTCCCg    <  1:3044253/148‑1 (MQ=255)
                                                                                                                                                  ccATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTACCACTCCCGTg  >  2:2461627/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTGCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAGTGGTTCCCGGGCGTGCGCATCTACCACTCCCGTG  >  CP009273/282336‑282629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑