Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,591,957 T→G 100% V354V (GTA→GTC)  livJ ← leucine/isoleucine/valine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,591,9570TG97.8% 909.8 / 1.5 226V354V (GTA→GTC) livJleucine/isoleucine/valine transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (2/3);  new base G (103/118);  total (105/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 226 total aligned reads displayed.

TTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAG  >  CP009273/3591812‑3592100
                                                                                                                                                 |                                                                                                                                               
tttGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCTGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGAc                                                                                                                                               >  1:3487572/1‑148 (MQ=255)
      tCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGaa                                                                                                                                         <  1:1541028/148‑1 (MQ=255)
      tCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGaa                                                                                                                                         <  1:2622122/148‑1 (MQ=255)
       cTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAAc                                                                                                                                        >  1:985623/1‑148 (MQ=255)
         tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTc                                                                                                                                      >  2:791982/1‑148 (MQ=255)
             ggCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAg                                                                                                                                  >  1:1516230/1‑148 (MQ=255)
              gCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGc                                                                                                                                 <  1:3208399/148‑1 (MQ=255)
                  gATCAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCttt                                                                                                                             <  1:1691076/148‑1 (MQ=255)
                  gATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCttt                                                                                                                             <  1:709688/148‑1 (MQ=255)
                    tAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCa                                                                                                                           >  1:3617940/1‑148 (MQ=255)
                    tAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCa                                                                                                                           >  1:205451/1‑148 (MQ=255)
                      aGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGa                                                                                                                         <  2:1113773/148‑1 (MQ=255)
                      aGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGa                                                                                                                         >  2:933801/1‑148 (MQ=255)
                      aGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGa                                                                                                                         <  2:3294118/148‑1 (MQ=255)
                       ggCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGAt                                                                                                                        >  2:3659028/1‑148 (MQ=255)
                       ggCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACCCAAAGCCTTTCAGAt                                                                                                                        <  1:1387349/148‑1 (MQ=255)
                           tttACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGcc                                                                                                                    >  1:2429176/1‑148 (MQ=255)
                           tttACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGcc                                                                                                                    >  2:549498/1‑148 (MQ=255)
                            ttACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAACGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGAGCGCCt                                                                                                                   >  2:2357750/1‑148 (MQ=255)
                            ttACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCt                                                                                                                   >  1:1242240/1‑148 (MQ=255)
                            ttACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCt                                                                                                                   <  2:1683441/148‑1 (MQ=255)
                            ttACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCt                                                                                                                   <  2:2381971/148‑1 (MQ=255)
                                 ccgcATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTtctc                                                                                                              <  2:1585952/148‑1 (MQ=255)
                                 ccgcATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTtctc                                                                                                              >  2:2127106/1‑148 (MQ=255)
                                 ccgcATCCGGCATGAAGCAACGTACTCGATATTAGCAATTCGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTtctc                                                                                                              <  2:1025643/148‑1 (MQ=255)
                                   gcATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCAt                                                                                                            <  2:1951594/148‑1 (MQ=255)
                                    cATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATc                                                                                                           >  2:2997139/1‑148 (MQ=255)
                                    cATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATc                                                                                                           >  1:1923057/1‑148 (MQ=255)
                                     aTCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATcc                                                                                                          <  2:285938/148‑1 (MQ=255)
                                       ccGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCa                                                                                                        <  2:1320713/148‑1 (MQ=255)
                                           cATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTc                                                                                                    >  2:2760907/1‑148 (MQ=255)
                                             tGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAg                                                                                                  >  1:2972430/1‑148 (MQ=255)
                                             tGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAg                                                                                                  >  1:296082/1‑148 (MQ=255)
                                               aaGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCg                                                                                                >  1:1118407/1‑148 (MQ=255)
                                               aaGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCg                                                                                                >  2:3425749/1‑148 (MQ=255)
                                               aaGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCg                                                                                                >  1:2379200/1‑148 (MQ=255)
                                               aaGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCg                                                                                                >  2:2688102/1‑148 (MQ=255)
                                                 gCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGt                                                                                              <  1:3463204/148‑1 (MQ=255)
                                                  cAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTc                                                                                             <  1:399428/148‑1 (MQ=255)
                                                  cAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTc                                                                                             <  1:3367205/148‑1 (MQ=255)
                                                     cGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCa                                                                                          >  2:1448323/1‑148 (MQ=255)
                                                     cGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCa                                                                                          >  2:3352170/1‑148 (MQ=255)
                                                       tACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCAtt                                                                                        <  2:2495025/148‑1 (MQ=255)
                                                       tACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCAtt                                                                                        <  1:590526/148‑1 (MQ=255)
                                                       tACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCAtt                                                                                        >  1:1790144/1‑148 (MQ=255)
                                                          tCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACg                                                                                     <  2:2908185/148‑1 (MQ=255)
                                                           cGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACgg                                                                                    <  2:2502516/148‑1 (MQ=255)
                                                           cGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACgg                                                                                    <  2:3087663/148‑1 (MQ=255)
                                                             atatTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTa                                                                                  <  2:490608/148‑1 (MQ=255)
                                                               atTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATc                                                                                >  2:2681240/1‑148 (MQ=255)
                                                               atTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATc                                                                                >  2:686915/1‑148 (MQ=255)
                                                               atTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATc                                                                                <  2:1433593/148‑1 (MQ=255)
                                                                 tAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCAATACGGTATCCa                                                                              >  2:2880949/1‑148 (MQ=255)
                                                                  aGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCAc                                                                             >  2:1190209/1‑148 (MQ=255)
                                                                    cAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACgg                                                                           <  1:950707/148‑1 (MQ=255)
                                                                    cAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACgg                                                                           >  1:530571/1‑148 (MQ=255)
                                                                     aaTTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGa                                                                          <  2:2906957/148‑1 (MQ=255)
                                                                       tttGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGt                                                                        <  2:2893841/148‑1 (MQ=255)
                                                                       tttGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGt                                                                        <  1:3659040/148‑1 (MQ=255)
                                                                        ttGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGtt                                                                       >  2:2652960/1‑148 (MQ=255)
                                                                         tGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTc                                                                      <  1:341339/148‑1 (MQ=255)
                                                                                  cccAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTCTCCACGGAGTTCGCTTTCAgg                                                             >  2:575871/1‑148 (MQ=255)
                                                                                  cccAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAgg                                                             >  2:3604228/1‑148 (MQ=255)
                                                                                  cccAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAgg                                                             <  2:3534829/148‑1 (MQ=255)
                                                                                  cccAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAgg                                                             >  1:91706/1‑148 (MQ=255)
                                                                                  cccAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAgg                                                             >  2:3260476/1‑148 (MQ=255)
                                                                                      aaGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTAtt                                                         <  1:2136186/148‑1 (MQ=255)
                                                                                      aaGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTAtt                                                         <  1:2136201/148‑1 (MQ=255)
                                                                                       aGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTAttt                                                        <  1:2563449/148‑1 (MQ=255)
                                                                                       aGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTAttt                                                        <  1:2563022/148‑1 (MQ=255)
                                                                                               aTTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGAttt                                                <  2:425433/148‑1 (MQ=255)
                                                                                                ttAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTc                                               >  2:1141719/1‑148 (MQ=255)
                                                                                                ttAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTc                                               >  1:1410143/1‑148 (MQ=255)
                                                                                                ttAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTc                                               >  1:1105539/1‑148 (MQ=255)
                                                                                                 tAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCa                                              >  1:2433234/1‑148 (MQ=255)
                                                                                                 tAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCa                                              >  1:3342306/1‑148 (MQ=255)
                                                                                                      aTTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCgg                                         >  2:2454764/1‑148 (MQ=255)
                                                                                                      aTTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCgg                                         <  2:2356471/148‑1 (MQ=255)
                                                                                                       ttACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGa                                        <  2:498153/148‑1 (MQ=255)
                                                                                                        tACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGAt                                       <  2:532338/148‑1 (MQ=255)
                                                                                                        tACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGAt                                       <  2:955839/148‑1 (MQ=255)
                                                                                                         aCTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATc                                      >  2:2306712/1‑148 (MQ=255)
                                                                                                               cATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGa                                <  2:69618/148‑1 (MQ=255)
                                                                                                                aTCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGAc                               >  1:2654465/1‑148 (MQ=255)
                                                                                                                 tCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACt                              >  1:2504767/1‑148 (MQ=255)
                                                                                                                  cGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTg                             >  1:176388/1‑148 (MQ=255)
                                                                                                                  cGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTg                             >  1:707334/1‑148 (MQ=255)
                                                                                                                  cGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTg                             >  2:3509492/1‑148 (MQ=255)
                                                                                                                   ggTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGa                            <  1:803375/148‑1 (MQ=255)
                                                                                                                    gTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGAt                           >  2:3794616/1‑148 (MQ=255)
                                                                                                                    gTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGAt                           <  1:1771168/148‑1 (MQ=255)
                                                                                                                       gccgtgccgtTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCa                        <  1:3015529/148‑1 (MQ=255)
                                                                                                                        ccgtgccgtTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAg                       <  1:1112187/148‑1 (MQ=255)
                                                                                                                           tgccgtTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGcc                    >  2:73603/1‑148 (MQ=255)
                                                                                                                              cgtTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGc                 <  1:397089/148‑1 (MQ=255)
                                                                                                                                    cGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCaa           <  2:530557/148‑1 (MQ=255)
                                                                                                                                       gCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGa        <  1:2760907/148‑1 (MQ=255)
                                                                                                                                          aGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTg     <  2:869502/148‑1 (MQ=255)
                                                                                                                                           gTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGc    <  2:1735165/148‑1 (MQ=255)
                                                                                                                                            tCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCa   <  1:3566375/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAG  >  CP009273/3591812‑3592100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑