Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,596,266 C→A 100% T446T (ACG→ACT)  ftsY ← Signal Recognition Particle (SRP) receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,596,2660CA97.7% 854.6 / 8.3 271T446T (ACG→ACT) ftsYSignal Recognition Particle (SRP) receptor
Reads supporting (aligned to +/- strand):  ref base C (3/4);  new base A (147/117);  total (150/121)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 273 total aligned reads displayed.

GGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTC  >  CP009273/3596121‑3596410
                                                                                                                                                 |                                                                                                                                                
gggCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGt                                                                                                                                                <  1:2889912/148‑1 (MQ=255)
gggCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGt                                                                                                                                                <  1:807667/148‑1 (MQ=255)
   cAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTttagttag                                                                                                                                             <  1:1795775/148‑1 (MQ=255)
     aaaaGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCg                                                                                                                                           >  2:3371429/1‑148 (MQ=255)
        aGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGa                                                                                                                                        <  2:3583360/148‑1 (MQ=255)
        aGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGa                                                                                                                                        <  1:2909994/148‑1 (MQ=255)
           gCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGc                                                                                                                                     <  1:1845937/148‑1 (MQ=255)
             ctctATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCg                                                                                                                                   <  1:1178552/148‑1 (MQ=255)
               ctATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGt                                                                                                                                 >  1:2748053/1‑148 (MQ=255)
               ctATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGt                                                                                                                                 >  1:1941099/1‑148 (MQ=255)
               ctATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGt                                                                                                                                 >  1:2254240/1‑148 (MQ=255)
                 ataAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTa                                                                                                                               >  2:3162029/1‑148 (MQ=255)
                  taAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTaa                                                                                                                              >  2:2364753/1‑148 (MQ=255)
                   aaaGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAg                                                                                                                             <  1:949037/148‑1 (MQ=255)
                   aaaGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAg                                                                                                                             <  1:499195/148‑1 (MQ=255)
                   aaaGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAg                                                                                                                             >  1:3447241/1‑148 (MQ=255)
                      gtcgtcCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCa                                                                                                                          >  2:3523036/1‑148 (MQ=255)
                      gtcgtcCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCa                                                                                                                          >  1:1732005/1‑148 (MQ=255)
                        cgtcCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAACATTACCCCGCCTTTCTCCGTGCCGACCCGTTTAGTTAGCGTGATGCCGGTTAAGCCAAc                                                                                                                        >  2:1954336/1‑148 (MQ=255)
                        cgtcCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTACGCCAAc                                                                                                                        >  2:2103127/1‑148 (MQ=255)
                        cgtcCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAAc                                                                                                                        <  1:2088504/148‑1 (MQ=255)
                           ccGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGc                                                                                                                     <  1:874053/148‑1 (MQ=255)
                           ccGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGc                                                                                                                     <  1:1466744/148‑1 (MQ=255)
                                ttAAACGGACGCAAATCCTCACTACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCAt                                                                                                                <  2:3355142/148‑1 (MQ=255)
                                ttAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCAt                                                                                                                >  1:3558235/1‑148 (MQ=255)
                                 tAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATg                                                                                                               >  2:32304/1‑148 (MQ=255)
                                 tAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTGCATg                                                                                                               >  2:1429197/1‑148 (MQ=255)
                                   aaCGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGa                                                                                                             <  1:3096030/148‑1 (MQ=255)
                                       gACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAg                                                                                                         <  2:1866327/148‑1 (MQ=255)
                                       gACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAg                                                                                                         >  1:2631491/1‑148 (MQ=255)
                                         cGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGtt                                                                                                       >  1:2690318/1‑148 (MQ=255)
                                          gCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGttt                                                                                                      <  1:3178225/148‑1 (MQ=255)
                                           cAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTg                                                                                                     >  1:2376027/1‑148 (MQ=255)
                                            aaaTCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTgg                                                                                                    >  2:2135591/1‑148 (MQ=255)
                                             aaTCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGc                                                                                                   >  2:2400153/1‑148 (MQ=255)
                                              aTCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGcc                                                                                                  >  2:1991996/1‑148 (MQ=255)
                                              aTCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGcc                                                                                                  <  2:1299425/148‑1 (MQ=255)
                                              aTCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGcc                                                                                                  <  1:215482/148‑1 (MQ=255)
                                               tCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCt                                                                                                 >  1:3520355/1‑148 (MQ=255)
                                                ccTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTg                                                                                                >  1:5528/1‑148 (MQ=255)
                                                ccTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTg                                                                                                >  2:2704016/1‑148 (MQ=255)
                                                ccTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCAGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTg                                                                                                <  2:1577865/148‑1 (MQ=255)
                                                   cAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCt                                                                                             >  2:3271321/1‑148 (MQ=255)
                                                   cAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCt                                                                                             >  1:901375/1‑148 (MQ=255)
                                                     aTACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTa                                                                                           >  2:508945/1‑148 (MQ=255)
                                                      tACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTAc                                                                                          >  2:1852819/1‑148 (MQ=255)
                                                       aCGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTAcc                                                                                         >  1:1075638/1‑148 (MQ=255)
                                                       aCGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTAcc                                                                                         >  2:449670/1‑148 (MQ=255)
                                                          ttCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACcgcg                                                                                      <  1:3780928/148‑1 (MQ=255)
                                                          gtCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACcgcg                                                                                      <  2:3291574/147‑1 (MQ=255)
                                                                gACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGc                                                                                <  2:2254188/148‑1 (MQ=255)
                                                                 acacCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGcc                                                                               <  1:7107/148‑1 (MQ=255)
                                                                      aaTGTAGCTGATATGGATACCAAACTGGTCAGCCACAGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTg                                                                          <  1:1331056/148‑1 (MQ=255)
                                                                      aaTGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTg                                                                          <  1:3234899/148‑1 (MQ=255)
                                                                        tGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTGTGGCCTGGCTTACCGCGTTCTGCCCGGTGCt                                                                        >  2:2426249/1‑148 (MQ=255)
                                                                             cGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCAt                                                                   <  2:2573583/148‑1 (MQ=255)
                                                                               gATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCa                                                                 <  2:1686822/148‑1 (MQ=255)
                                                                               gATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCa                                                                 <  2:3558235/148‑1 (MQ=255)
                                                                                  aGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATa                                                              <  1:2364749/148‑1 (MQ=255)
                                                                                  aGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATa                                                              <  1:508945/148‑1 (MQ=255)
                                                                                  aGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATa                                                              >  2:582985/1‑148 (MQ=255)
                                                                                    ggATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGACCAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGt                                                            >  2:1353195/1‑148 (MQ=255)
                                                                                     gATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTc                                                           >  2:617376/1‑148 (MQ=255)
                                                                                      aTACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCa                                                          >  2:2807001/1‑148 (MQ=255)
                                                                                            aaCTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATaa                                                    <  1:449670/148‑1 (MQ=255)
                                                                                             aCTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAAc                                                   <  1:3162029/148‑1 (MQ=255)
                                                                                             aCTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAAc                                                   >  1:2919942/1‑148 (MQ=255)
                                                                                             aCTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAAc                                                   >  1:1911957/1‑148 (MQ=255)
                                                                                             aCTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCAGCCCGGTGCTGGCATCAATAGTCAGCATAAc                                                   >  1:1161496/1‑148 (MQ=255)
                                                                                              cTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACt                                                  >  1:2595716/1‑148 (MQ=255)
                                                                                                ggTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTc                                                >  1:2894099/1‑148 (MQ=255)
                                                                                                 gTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCa                                               >  2:2213711/1‑148 (MQ=255)
                                                                                                  tCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCAt                                              >  1:2916885/1‑148 (MQ=255)
                                                                                                   cAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATg                                             <  2:207850/148‑1 (MQ=255)
                                                                                                      ccACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATgcgg                                          >  2:2892887/1‑148 (MQ=255)
                                                                                                      ccACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATgcgg                                          >  1:3396361/1‑148 (MQ=255)
                                                                                                             gaAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCa                                   >  2:400078/1‑148 (MQ=255)
                                                                                                                 aTTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGt                               >  2:767024/1‑148 (MQ=255)
                                                                                                                 aTTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGt                               >  2:3043458/1‑148 (MQ=255)
                                                                                                                  ttACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTc                              >  2:1338356/1‑148 (MQ=255)
                                                                                                                  ttACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTc                              >  2:2749935/1‑148 (MQ=255)
                                                                                                                  ttACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTc                              >  2:2750106/1‑148 (MQ=255)
                                                                                                                   tACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTAACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCg                             >  2:2547172/1‑148 (MQ=255)
                                                                                                                    aCCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTTATCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGa                            <  2:419653/148‑1 (MQ=255)
                                                                                                                     ccccGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAg                           >  1:1627486/1‑148 (MQ=255)
                                                                                                                        cGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGttt                        <  2:1083381/148‑1 (MQ=255)
                                                                                                                         gCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttc                       <  2:2613484/148‑1 (MQ=255)
                                                                                                                         gCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttc                       >  1:2843343/1‑148 (MQ=255)
                                                                                                                          ccTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttct                      >  2:2957741/1‑148 (MQ=255)
                                                                                                                          ccTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttct                      >  1:2502985/1‑148 (MQ=255)
                                                                                                                           cTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttctt                     <  2:252797/148‑1 (MQ=255)
                                                                                                                           cTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttctt                     >  1:3300502/1‑148 (MQ=255)
                                                                                                                           cTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttctt                     <  2:946229/148‑1 (MQ=255)
                                                                                                                            tttCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttcttc                    <  1:71221/148‑1 (MQ=255)
                                                                                                                            tttCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttcttc                    <  2:823270/148‑1 (MQ=255)
                                                                                                                                gccgtgccgtCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTtcatca                <  1:3279409/148‑1 (MQ=255)
                                                                                                                                   gtgccgtCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGACTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCAcgc             <  2:694907/148‑1 (MQ=255)
                                                                                                                                     gccgtCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCgg           <  1:3715554/148‑1 (MQ=255)
                                                                                                                                          ccAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAt      <  2:2916885/148‑1 (MQ=255)
                                                                                                                                          ccAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAt      >  1:590925/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTC  >  CP009273/3596121‑3596410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑