Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,604,608 C→A 22.9% A162A (GCG→GCT)  yhhS ← putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,604,6080CA22.9% 513.4 / 104.9 204A162A (GCG→GCT) yhhSputative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base C (57/100);  new base A (29/17);  total (86/118)
Fisher's exact test for biased strand distribution p-value = 2.04e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 233 total aligned reads displayed.

AGCGGTTTGCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGTAATAACAGG  >  CP009273/3604462‑3604755
                                                                                                                                                  |                                                                                                                                                   
  cGGTTTGCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCatcgccatcgcc                                                                                                                                                  >  2:2524860/1‑148 (MQ=255)
  cGGTTTCCCTTTACTGGCTTTTACCGACGCCCGCGGGATCCCCAACAAAATGGCCACCCCCCCCACCCCCAAAATGATAACCGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCatcgccatcgcc                                                                                                                                                  <  1:386101/148‑1 (MQ=255)
   ggTTTGCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGccc                                                                                                                                                 <  2:2725764/148‑1 (MQ=255)
    gTTTGCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGcccc                                                                                                                                                >  1:2513140/1‑148 (MQ=255)
     tttGCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCg                                                                                                                                               <  2:265585/148‑1 (MQ=255)
     tttGCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCg                                                                                                                                               >  2:1432599/1‑148 (MQ=255)
      ttGCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGt                                                                                                                                              >  2:1070834/1‑148 (MQ=255)
      ttGCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGt                                                                                                                                              >  2:1093926/1‑148 (MQ=255)
          cTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGt                                                                                                                                          <  2:1024075/148‑1 (MQ=255)
            ttACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGa                                                                                                                                        <  2:1594617/148‑1 (MQ=255)
              aCTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACa                                                                                                                                      <  2:758939/148‑1 (MQ=255)
                 ggCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATg                                                                                                                                   >  2:192176/1‑148 (MQ=255)
                    ttttACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCg                                                                                                                                <  1:130301/148‑1 (MQ=255)
                    ttttACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCg                                                                                                                                <  1:2059083/148‑1 (MQ=255)
                     tttACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGt                                                                                                                               >  1:2660514/1‑148 (MQ=255)
                       tACCGTGGGCGGCGTGATCGAAAAAAAAATGGCCACCTGCCCCACGCCAATAATGATAACGGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTc                                                                                                                             <  1:1335205/148‑1 (MQ=255)
                       tACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTc                                                                                                                             <  1:3657598/148‑1 (MQ=255)
                       tACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTc                                                                                                                             <  2:2739716/148‑1 (MQ=255)
                             cGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGaa                                                                                                                       <  2:3552461/148‑1 (MQ=255)
                               gACGCGGGATCGCAAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAAt                                                                                                                     <  1:2593121/148‑1 (MQ=255)
                                aCGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATc                                                                                                                    <  2:3252519/148‑1 (MQ=255)
                                aCGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATc                                                                                                                    >  1:3272998/1‑148 (MQ=255)
                                 cgcgGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCa                                                                                                                   <  1:1241499/148‑1 (MQ=255)
                                      gATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCAcccgc                                                                                                              >  2:100211/1‑148 (MQ=255)
                                       aTCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCAcccgcc                                                                                                             <  2:463030/148‑1 (MQ=255)
                                        tCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCAcccgccc                                                                                                            >  2:2092745/1‑148 (MQ=255)
                                           caaacaaAAGCGCCACCCGCGCAACGCCCTTAATTATTCACGCTAACGCCTCCAAGCCGCCCCACTGATAAAACACGAAGCCTAACGGCGCAACCATAGCCATCGCCCCGTAAGTGACAATGCCTTTCCACTATATCACCCGCCCGat                                                                                                         <  1:721724/146‑1 (MQ=255)
                                            caacaaAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGata                                                                                                        <  1:530841/148‑1 (MQ=255)
                                              acaaAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATg                                                                                                      >  1:1386465/1‑148 (MQ=255)
                                              acaaAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATg                                                                                                      >  1:735719/1‑148 (MQ=255)
                                                  aaTGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGc                                                                                                  >  1:2827730/1‑148 (MQ=255)
                                                    tGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGa                                                                                                >  1:760917/1‑148 (MQ=255)
                                                    tGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGa                                                                                                <  1:3101107/148‑1 (MQ=255)
                                                      gCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGGCGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGc                                                                                              <  1:2880650/148‑1 (MQ=255)
                                                          ccaGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAAc                                                                                          >  2:1366416/1‑148 (MQ=255)
                                                          ccaGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAAc                                                                                          >  1:101349/1‑148 (MQ=255)
                                                           caGCGCCACGCCCATAATGATGAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAAcc                                                                                         <  2:1611070/148‑1 (MQ=255)
                                                               gcCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGc                                                                                     <  2:1840617/148‑1 (MQ=255)
                                                                     cccATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGc                                                                               >  1:1117357/1‑148 (MQ=255)
                                                                       cATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGccc                                                                             <  2:2154380/148‑1 (MQ=255)
                                                                       cATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGccc                                                                             <  2:2743776/148‑1 (MQ=255)
                                                                       cATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGccc                                                                             >  2:2004587/1‑148 (MQ=255)
                                                                        aTAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGcccc                                                                            >  2:1381566/1‑148 (MQ=255)
                                                                         tAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCa                                                                           <  2:3165880/148‑1 (MQ=255)
                                                                          aaTGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCAt                                                                          <  1:2149064/148‑1 (MQ=255)
                                                                            tGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAg                                                                        >  1:2312487/1‑148 (MQ=255)
                                                                             gATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAgg                                                                       >  1:464159/1‑148 (MQ=255)
                                                                              aTTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAggg                                                                      <  2:3150445/148‑1 (MQ=255)
                                                                              aTTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAggg                                                                      <  2:2536488/148‑1 (MQ=255)
                                                                              aTTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAggg                                                                      <  2:1772401/148‑1 (MQ=255)
                                                                               ttAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACTACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGATCCACGAAATCACACGCCCGATATGCATCGAGCCAACCACGCAAACGCCCCATAggtt                                                                     >  1:1643453/1‑146 (MQ=255)
                                                                               ttAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGt                                                                     <  1:507576/148‑1 (MQ=255)
                                                                               ttAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGt                                                                     >  1:3539445/1‑148 (MQ=255)
                                                                                  acgctaacgcCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGa                                                                  <  1:2096307/148‑1 (MQ=255)
                                                                                   cgctaacgcCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGAt                                                                 <  1:3415956/148‑1 (MQ=255)
                                                                                    gctaacgcCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATc                                                                <  2:825147/148‑1 (MQ=255)
                                                                                    gctaacgcCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATc                                                                <  1:1531378/148‑1 (MQ=255)
                                                                                      taacgcCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATccc                                                              >  2:1202027/1‑148 (MQ=255)
                                                                                         cgcCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGtt                                                           >  2:3378817/1‑148 (MQ=255)
                                                                                         cgcCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGtt                                                           >  1:2169161/1‑148 (MQ=255)
                                                                                         cgcCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCAGAGGGTCGATCCCGtt                                                           >  2:2400991/1‑148 (MQ=255)
                                                                                           ccTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGAGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTcc                                                         >  2:1669288/1‑148 (MQ=255)
                                                                                                 aGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCaaa                                                   <  1:1858487/148‑1 (MQ=255)
                                                                                                 aGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCaaa                                                   >  1:2978197/1‑148 (MQ=255)
                                                                                                  gccgccCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCaaaa                                                  >  1:1851892/1‑148 (MQ=255)
                                                                                                  gccgccCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCaaaa                                                  <  2:2197270/148‑1 (MQ=255)
                                                                                                   ccgccCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAAc                                                 <  1:1788314/148‑1 (MQ=255)
                                                                                                       cccAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTg                                             <  1:202554/148‑1 (MQ=255)
                                                                                                         cAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGcc                                           <  1:2998447/148‑1 (MQ=255)
                                                                                                          aGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGccc                                          >  2:1144805/1‑148 (MQ=255)
                                                                                                            tGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCaa                                        <  1:362240/148‑1 (MQ=255)
                                                                                                              aTAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATc                                      <  1:3701782/148‑1 (MQ=255)
                                                                                                               tAAAACACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATcc                                     <  2:2296668/148‑1 (MQ=255)
                                                                                                                 aaaCACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCa                                   >  1:1356937/1‑148 (MQ=255)
                                                                                                                  aaCACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCATGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCaa                                  <  2:34827/148‑1 (MQ=255)
                                                                                                                  aaCACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCaa                                  <  1:531479/148‑1 (MQ=255)
                                                                                                                  aaCACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCaa                                  >  1:3498080/1‑148 (MQ=255)
                                                                                                                  aaCACGACGCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAAATTTGCCCAAACCCaa                                  >  1:32143/1‑148 (MQ=255)
                                                                                                                    cacGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAgg                                <  1:3190743/148‑1 (MQ=255)
                                                                                                                     acgacgCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGa                               <  2:3722709/148‑1 (MQ=255)
                                                                                                                      cgacgCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGAt                              >  1:3795986/1‑148 (MQ=255)
                                                                                                                       gacgCCTCACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATg                             <  2:3600436/148‑1 (MQ=255)
                                                                                                                       gacgCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATg                             <  1:22624/148‑1 (MQ=255)
                                                                                                                        acgCCTAACGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGa                            <  2:3350298/148‑1 (MQ=255)
                                                                                                                           ccTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGAcgc                         >  2:2629345/1‑148 (MQ=255)
                                                                                                                               aCGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGccc                     <  2:3599407/148‑1 (MQ=255)
                                                                                                                               aCGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGccc                     <  2:3744539/148‑1 (MQ=255)
                                                                                                                               aCGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGccc                     <  2:518634/148‑1 (MQ=255)
                                                                                                                               aCGGCGCACCCATCGCCATAGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGccc                     >  1:173022/1‑148 (MQ=255)
                                                                                                                                 ggCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCa                   <  2:729384/148‑1 (MQ=255)
                                                                                                                                     cACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCa               >  2:269775/1‑148 (MQ=255)
                                                                                                                                      aCCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCaa              <  2:3724384/148‑1 (MQ=255)
                                                                                                                                             gccatcgccCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGtaata       <  1:2353265/148‑1 (MQ=255)
                                                                                                                                               catcgccCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGTAATAAc     >  1:1567981/1‑148 (MQ=255)
                                                                                                                                                atcgccCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGTAATAACa    >  1:2746159/1‑148 (MQ=255)
                                                                                                                                                 tcgccCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGTAATAACAg   >  1:2462477/1‑148 (MQ=255)
                                                                                                                                                 tcgccCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGTAATAACAg   >  1:2459567/1‑148 (MQ=255)
                                                                                                                                                  cgccCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGTAATAACAgg  <  2:2181104/148‑1 (MQ=255)
                                                                                                                                                  cgccCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGTAATAACAgg  <  1:2629345/148‑1 (MQ=255)
                                                                                                                                                  cgccCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGTAATAACAgg  <  2:3814588/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AGCGGTTTGCCTTTACTGGCTTTTACCGTCGGACGCGGGATCGCCAACAAAATGGCCACCAGCGCCACGCCCATAATGATTAACGCTAACGCCTGCAAGCCGCCCCAGTGATAAAACACGACGCCTAACGGCGCACCCATCGCCATCGCCCCGTAAGTGACAATGCCGTTCCACGAAATCACCCGCCCGATATGCAGCGAGCCAACCACGCCAACGCCCCATAGGGTCGATCCCGTTCCGGCAAAACTTTGCCCAATCCCAAGGATGACGCGCCCCAGGCAAAGTAATAACAGG  >  CP009273/3604462‑3604755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑