Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,607,110 C→A 100% I28I (ATC→ATA)  nikA → nickel‑binding, heme‑binding periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,607,1100CA100.0% 634.6 / ‑6.7 189I28I (ATC→ATA) nikAnickel‑binding, heme‑binding periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (99/89);  minor base T (0/1);  total (99/90)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 198 total aligned reads displayed.

TGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGG  >  CP009273/3606965‑3607254
                                                                                                                                                 |                                                                                                                                                
tGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATaac                                                                                                                                                >  1:1070624/1‑148 (MQ=255)
 gACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATaacc                                                                                                                                               <  1:3711537/148‑1 (MQ=255)
  aCGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATaacca                                                                                                                                              <  2:2294501/148‑1 (MQ=255)
  aCGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATaacca                                                                                                                                              <  2:370985/148‑1 (MQ=255)
  aCGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATaacca                                                                                                                                              <  2:436229/148‑1 (MQ=255)
       tACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGcc                                                                                                                                         >  1:1450222/1‑148 (MQ=255)
           tAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGc                                                                                                                                     >  1:170585/1‑148 (MQ=255)
               aTCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGt                                                                                                                                 >  1:2757154/1‑148 (MQ=255)
                tCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTg                                                                                                                                >  2:617925/1‑148 (MQ=255)
                tCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTg                                                                                                                                >  1:1832139/1‑148 (MQ=255)
                tCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTg                                                                                                                                >  1:2856074/1‑148 (MQ=255)
                 cGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGa                                                                                                                               >  2:3403013/1‑148 (MQ=255)
                      tACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTc                                                                                                                          >  2:2147672/1‑148 (MQ=255)
                         ttATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCggg                                                                                                                       >  1:39164/1‑148 (MQ=255)
                          tATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGc                                                                                                                      <  2:1363077/148‑1 (MQ=255)
                          tATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGc                                                                                                                      >  1:848260/1‑148 (MQ=255)
                          tATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGc                                                                                                                      >  2:3635708/1‑148 (MQ=255)
                          tATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGc                                                                                                                      >  2:2720117/1‑148 (MQ=255)
                            tttCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCa                                                                                                                    >  1:1726834/1‑148 (MQ=255)
                            tttCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCa                                                                                                                    >  1:1195773/1‑148 (MQ=255)
                             ttCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCAc                                                                                                                   >  2:2051310/1‑148 (MQ=255)
                                   cATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAAcc                                                                                                             >  1:2061316/1‑148 (MQ=255)
                                   cATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAAcc                                                                                                             >  1:2062177/1‑148 (MQ=255)
                                       tATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCa                                                                                                         <  2:168810/148‑1 (MQ=255)
                                        aTTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCAc                                                                                                        >  1:3410978/1‑148 (MQ=255)
                                        aTTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCAc                                                                                                        <  1:1965963/148‑1 (MQ=255)
                                        aTTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCAc                                                                                                        >  1:2677166/1‑148 (MQ=255)
                                        aTTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCAc                                                                                                        >  1:2677341/1‑148 (MQ=255)
                                         ttttAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCAcc                                                                                                       <  1:1806203/148‑1 (MQ=255)
                                           ttAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCtt                                                                                                     >  1:3078506/1‑148 (MQ=255)
                                            tAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCttt                                                                                                    <  1:2080422/148‑1 (MQ=255)
                                              aTCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTac                                                                                                  >  1:1434047/1‑148 (MQ=255)
                                              aTCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTac                                                                                                  <  2:3179649/148‑1 (MQ=255)
                                               tCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTaca                                                                                                 >  1:1164764/1‑148 (MQ=255)
                                               tCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTaca                                                                                                 >  1:1547412/1‑148 (MQ=255)
                                                ccATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTacac                                                                                                >  2:2759252/1‑148 (MQ=255)
                                                    tGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCt                                                                                            >  1:2918517/1‑148 (MQ=255)
                                                     ggggTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTa                                                                                           >  2:3584502/1‑148 (MQ=255)
                                                      gggTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTaa                                                                                          <  1:3685355/148‑1 (MQ=255)
                                                      gggTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTaa                                                                                          <  1:182369/148‑1 (MQ=255)
                                                       ggTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAAc                                                                                         >  1:3596371/1‑148 (MQ=255)
                                                        gTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAAcc                                                                                        <  2:3447353/148‑1 (MQ=255)
                                                         ttACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCa                                                                                       >  2:2217616/1‑148 (MQ=255)
                                                          tACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAg                                                                                      >  1:2208873/1‑148 (MQ=255)
                                                           aCCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGa                                                                                     <  1:2397684/148‑1 (MQ=255)
                                                            ccATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGAt                                                                                    >  2:2833011/1‑148 (MQ=255)
                                                             cATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATg                                                                                   <  2:767365/148‑1 (MQ=255)
                                                              aTGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGt                                                                                  <  2:3596371/148‑1 (MQ=255)
                                                               tGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGtt                                                                                 >  2:2378224/1‑148 (MQ=255)
                                                                gCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTAAACGCCTAACCAGATGTTc                                                                                >  2:1106720/1‑148 (MQ=255)
                                                                   ctcCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGcc                                                                             >  2:2139849/1‑148 (MQ=255)
                                                                         acTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGc                                                                       <  2:1586847/148‑1 (MQ=255)
                                                                         acTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGc                                                                       >  1:1319616/1‑148 (MQ=255)
                                                                         acTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGc                                                                       >  1:1315669/1‑148 (MQ=255)
                                                                          cTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCa                                                                      <  1:437735/148‑1 (MQ=255)
                                                                          cTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCa                                                                      <  2:2727962/148‑1 (MQ=255)
                                                                            ccgccgCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCCGATGTTCGCCCAGAGCATg                                                                    <  1:2265429/148‑1 (MQ=255)
                                                                            ccgccgCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATg                                                                    <  1:339646/148‑1 (MQ=255)
                                                                               ccgcACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGtt                                                                 <  1:920794/148‑1 (MQ=255)
                                                                               ccgcACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGtt                                                                 <  2:1498036/148‑1 (MQ=255)
                                                                                cgcACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGttt                                                                <  2:3699916/148‑1 (MQ=255)
                                                                                 gcACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTa                                                               <  2:2954301/148‑1 (MQ=255)
                                                                                 gcACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTa                                                               <  1:721610/148‑1 (MQ=255)
                                                                                 gcACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTa                                                               <  1:720723/148‑1 (MQ=255)
                                                                                  cACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTAt                                                              >  2:2574712/1‑148 (MQ=255)
                                                                                  cACTCTATTTGCGCTGATGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGACCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTAt                                                              >  2:1611647/1‑148 (MQ=255)
                                                                                     tctATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGaa                                                           <  1:3476113/148‑1 (MQ=255)
                                                                                      ctATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAAc                                                          >  2:2656535/1‑148 (MQ=255)
                                                                                       tagttGCGCGGCTGGCGTGTGCGTCATTTATCGTCCATGCCGCTGCACCAGATGAAATAACCAGCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCTTGGTTTATGAAcc                                                         <  2:3012401/145‑1 (MQ=255)
                                                                                          ttGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCAtt                                                      <  1:330941/148‑1 (MQ=255)
                                                                                               ctgctgGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGa                                                 >  1:3018002/1‑148 (MQ=255)
                                                                                                tgctgGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGaa                                                <  1:1682774/148‑1 (MQ=255)
                                                                                                tgctgGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGaa                                                <  1:2478696/148‑1 (MQ=255)
                                                                                                ggcgggCGTGTGCGTCTTGTATCCTCCAGGCCGCTGCACCAGATGAAATAACCACCGCCAGGCCGGTGAAGGTCGGGCCACTACACCCGCACCTTTACACGCCTAACCAGAGGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGaa                                                <  2:336603/144‑1 (MQ=255)
                                                                                                  ctgGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAat                                              <  2:1401883/148‑1 (MQ=255)
                                                                                                  ctgGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAat                                              <  2:3036138/148‑1 (MQ=255)
                                                                                                  ctgGCGTGTGCGTCTTTTATCGTCCATTCCGCTGCACCAGATGAAATTACCACCGCCTGGCCGGTGATTGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGTACCATTGGTGAAat                                              <  2:1713588/148‑1 (MQ=255)
                                                                                                     gCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATc                                           <  1:3288607/148‑1 (MQ=255)
                                                                                                         gtgCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGc                                       <  2:1168297/148‑1 (MQ=255)
                                                                                                                 tttATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGtt                               >  2:2847117/1‑148 (MQ=255)
                                                                                                                      cGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTg                          <  1:1978495/148‑1 (MQ=255)
                                                                                                                         ccATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATc                       >  1:1657499/1‑148 (MQ=255)
                                                                                                                            tGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGACTGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCg                    <  2:78990/148‑1 (MQ=255)
                                                                                                                            tGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCg                    >  2:68644/1‑148 (MQ=255)
                                                                                                                             gCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGt                   >  2:2020291/1‑148 (MQ=255)
                                                                                                                               cGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGtgg                 >  2:2487966/1‑148 (MQ=255)
                                                                                                                                   gCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGtggctgg             >  1:1330709/1‑148 (MQ=255)
                                                                                                                                   gCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGtggctgg             <  2:1315669/148‑1 (MQ=255)
                                                                                                                                      ccAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCaa          <  1:1107748/148‑1 (MQ=255)
                                                                                                                                      ccAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCaa          >  2:3147895/1‑148 (MQ=255)
                                                                                                                                      ccAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCaa          >  1:3003099/1‑148 (MQ=255)
                                                                                                                                         gATGAAATAACCACCGCCTGGCCAGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCaaaaa       >  1:1546840/1‑148 (MQ=255)
                                                                                                                                          aTGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAg      <  1:495209/148‑1 (MQ=255)
                                                                                                                                          aTGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAg      >  1:1614006/1‑148 (MQ=255)
                                                                                                                                          aTGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAg      >  1:3509123/1‑148 (MQ=255)
                                                                                                                                          aTGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAg      >  2:20870/1‑148 (MQ=255)
                                                                                                                                          aTGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAg      >  2:21043/1‑148 (MQ=255)
                                                                                                                                            gAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCt    <  1:2346266/148‑1 (MQ=255)
                                                                                                                                            gAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCt    >  1:557345/1‑148 (MQ=255)
                                                                                                                                            gAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCt    <  2:2210900/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTGG  >  CP009273/3606965‑3607254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑