Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 288,249 T→A 100% T137T (ACA→ACT)  yagK ← CP4‑6 prophage; conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273288,2490TA98.3% 775.2 / 1.8 234T137T (ACA→ACT) yagKCP4‑6 prophage; conserved protein
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base A (104/126);  total (105/129)
Fisher's exact test for biased strand distribution p-value = 6.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 238 total aligned reads displayed.

CAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAGGGCAAC  >  CP009273/288102‑288391
                                                                                                                                                   |                                                                                                                                              
cAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCt                                                                                                                                                >  1:3759003/1‑148 (MQ=255)
      gTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGatc                                                                                                                                          <  1:2954788/148‑1 (MQ=255)
      gTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGatc                                                                                                                                          <  2:1663522/148‑1 (MQ=255)
       tCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGatca                                                                                                                                         >  2:2740101/1‑148 (MQ=255)
       tCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGatca                                                                                                                                         <  1:3163157/148‑1 (MQ=255)
         cAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGatcatc                                                                                                                                       <  1:1791444/148‑1 (MQ=255)
          aGTCGGGTTAACTGCGCCTGTTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATcc                                                                                                                                      <  2:1137412/148‑1 (MQ=255)
          aGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATcc                                                                                                                                      <  2:1648210/148‑1 (MQ=255)
           gTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATccc                                                                                                                                     <  1:2524312/148‑1 (MQ=255)
             cGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCctc                                                                                                                                   >  2:3493029/1‑148 (MQ=255)
               ggTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTa                                                                                                                                 <  1:3604484/148‑1 (MQ=255)
                gTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTaa                                                                                                                                <  2:1596801/148‑1 (MQ=255)
                 ttAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAg                                                                                                                               <  1:877430/148‑1 (MQ=255)
                   aaCAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGTGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGt                                                                                                                             <  1:2605263/148‑1 (MQ=255)
                   aaCAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGt                                                                                                                             <  1:3309753/148‑1 (MQ=255)
                    aCAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGtt                                                                                                                            >  2:3806806/1‑148 (MQ=255)
                    aCAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGtt                                                                                                                            <  2:1968976/148‑1 (MQ=255)
                      aGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTAt                                                                                                                          <  1:1069073/148‑1 (MQ=255)
                      aGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTAt                                                                                                                          <  1:341817/148‑1 (MQ=255)
                      aGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTAt                                                                                                                          <  2:3608211/148‑1 (MQ=255)
                        cgcCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCg                                                                                                                        >  1:3381343/1‑148 (MQ=255)
                          ccTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTc                                                                                                                      <  2:550096/148‑1 (MQ=255)
                             ggTAGTTCTGCTGGTAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTg                                                                                                                   <  1:1860607/148‑1 (MQ=255)
                             ggTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTg                                                                                                                   >  1:1112171/1‑148 (MQ=255)
                             ggTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTg                                                                                                                   >  2:3439039/1‑148 (MQ=255)
                               tAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGAt                                                                                                                 >  1:854050/1‑148 (MQ=255)
                               tAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGAt                                                                                                                 >  2:184863/1‑148 (MQ=255)
                                   tCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTa                                                                                                             >  1:2921903/1‑148 (MQ=255)
                                    ctgctgGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTaa                                                                                                            <  2:138212/148‑1 (MQ=255)
                                    ctgctgGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTaa                                                                                                            <  1:3241579/148‑1 (MQ=255)
                                     tgctgGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAAt                                                                                                           >  2:2812725/1‑148 (MQ=255)
                                     tgctgGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAAt                                                                                                           <  2:1320320/148‑1 (MQ=255)
                                      gctgGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATc                                                                                                          <  2:2593935/148‑1 (MQ=255)
                                         ggAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATcccc                                                                                                       >  2:2104306/1‑148 (MQ=255)
                                          gAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCg                                                                                                      >  1:675382/1‑148 (MQ=255)
                                           aaGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGa                                                                                                     <  1:433179/148‑1 (MQ=255)
                                              tCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAggt                                                                                                  >  1:807131/1‑148 (MQ=255)
                                              tCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAggt                                                                                                  >  1:1229874/1‑148 (MQ=255)
                                               cAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAggtg                                                                                                 >  1:2013076/1‑148 (MQ=255)
                                                 gTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAggtggt                                                                                               <  1:261300/148‑1 (MQ=255)
                                                    cTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGgtagt                                                                                            <  2:665681/148‑1 (MQ=255)
                                                          gtgtCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTAACGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGt                                                                                      >  1:1568924/1‑148 (MQ=255)
                                                           tgtCTAGCACATACTGACCGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTc                                                                                     <  2:3360303/148‑1 (MQ=255)
                                                            gtCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCt                                                                                    <  1:701454/148‑1 (MQ=255)
                                                             tCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCtt                                                                                   <  2:605186/148‑1 (MQ=255)
                                                               tAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTttg                                                                                 <  1:310627/148‑1 (MQ=255)
                                                               tAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTttg                                                                                 <  2:3147994/148‑1 (MQ=255)
                                                               tAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTttg                                                                                 <  2:1031909/148‑1 (MQ=255)
                                                                   acaTACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGa                                                                             <  2:3110404/148‑1 (MQ=255)
                                                                   acaTACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGa                                                                             <  1:1912314/148‑1 (MQ=255)
                                                                       aCTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGaacaa                                                                         >  1:546987/1‑148 (MQ=255)
                                                                       aCTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGaacaa                                                                         >  1:2679931/1‑148 (MQ=255)
                                                                         ttACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAacaaca                                                                       >  1:2787552/1‑148 (MQ=255)
                                                                          tACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAg                                                                      >  1:759133/1‑148 (MQ=255)
                                                                           aCAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAgg                                                                     <  1:2326129/148‑1 (MQ=255)
                                                                              gTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCaa                                                                  >  1:3633784/1‑148 (MQ=255)
                                                                               ttttCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCaaa                                                                 >  2:1785385/1‑148 (MQ=255)
                                                                                tttCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAAt                                                                <  2:2484435/148‑1 (MQ=255)
                                                                                   cAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAAtgtg                                                             <  2:3676546/148‑1 (MQ=255)
                                                                                   cAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAAtgtg                                                             <  2:1357902/148‑1 (MQ=255)
                                                                                    aGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGa                                                            <  2:2128600/148‑1 (MQ=255)
                                                                                    aGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGa                                                            <  2:3765271/148‑1 (MQ=255)
                                                                                     ggAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGAt                                                           >  1:1368884/1‑148 (MQ=255)
                                                                                     ggAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGAt                                                           >  1:2439891/1‑148 (MQ=255)
                                                                                      gAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATa                                                          >  1:1621343/1‑148 (MQ=255)
                                                                                       aaaaTGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAg                                                         >  1:515061/1‑148 (MQ=255)
                                                                                          aTGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTgg                                                      >  2:2256213/1‑148 (MQ=255)
                                                                                          aTGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTgg                                                      >  1:89604/1‑148 (MQ=255)
                                                                                           tGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGc                                                     >  1:540230/1‑148 (MQ=255)
                                                                                            ggACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCa                                                    <  2:1817467/148‑1 (MQ=255)
                                                                                            ggACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCa                                                    <  1:2427361/148‑1 (MQ=255)
                                                                                             gaccagaccaGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCAt                                                   >  1:2476288/1‑148 (MQ=255)
                                                                                             gaccagaccaGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCAt                                                   >  1:2537929/1‑148 (MQ=255)
                                                                                             gaccagaccaGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCAt                                                   >  1:1475436/1‑148 (MQ=255)
                                                                                               ccagaccagGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCAttt                                                 <  2:2503590/148‑1 (MQ=255)
                                                                                               ccagaccagGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCAttt                                                 <  2:1796726/148‑1 (MQ=255)
                                                                                                 agaccagGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTAc                                               <  2:854050/148‑1 (MQ=255)
                                                                                                 agaccagGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTAc                                               <  2:2944879/148‑1 (MQ=255)
                                                                                                      agGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGAt                                          >  1:1201779/1‑148 (MQ=255)
                                                                                                        ggTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTc                                        >  2:2341645/1‑148 (MQ=255)
                                                                                                        ggTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTc                                        >  1:2159717/1‑148 (MQ=255)
                                                                                                          tGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAg                                      >  2:2534870/1‑148 (MQ=255)
                                                                                                            aTCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGaa                                    >  2:968227/1‑148 (MQ=255)
                                                                                                            aTCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGaa                                    >  2:3212413/1‑148 (MQ=255)
                                                                                                                 ctctctcCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATActc                               >  2:420893/1‑148 (MQ=255)
                                                                                                                          gTCTCAGGGCGCTGTACAAGGCTCCAGTGATCATCCCTCTACGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTTAGCGTCTTTGTTGAACTACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCa                      <  2:2669333/148‑1 (MQ=255)
                                                                                                                           tctcAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAAGAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCat                     >  2:2368468/1‑148 (MQ=255)
                                                                                                                           tctcAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCat                     <  1:217239/148‑1 (MQ=255)
                                                                                                                           tctcAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCat                     <  1:1907249/148‑1 (MQ=255)
                                                                                                                            ctcAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCata                    >  1:143454/1‑148 (MQ=255)
                                                                                                                              cAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCAtata                  >  1:3489131/1‑148 (MQ=255)
                                                                                                                                  gcgcTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATAtaataa              <  2:704997/148‑1 (MQ=255)
                                                                                                                                   cgcTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATaataaa             <  1:3262543/148‑1 (MQ=255)
                                                                                                                                    gcTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATaataaat            <  2:1191962/148‑1 (MQ=255)
                                                                                                                                      tGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAataaataa          <  1:2261398/148‑1 (MQ=255)
                                                                                                                                       gTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAAtaaataaa         <  1:2055722/148‑1 (MQ=255)
                                                                                                                                        tACGAGTCTCCAGTGATCATCCCTATAAGGTTATCGTCCTTATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAg        <  1:3141581/148‑1 (MQ=255)
                                                                                                                                         aCCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAgg       <  2:471995/148‑1 (MQ=255)
                                                                                                                                         aCCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAgg       >  1:1060632/1‑148 (MQ=255)
                                                                                                                                             ggCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTTATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAGGGCaa   <  1:2740101/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
CAGGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAGGGCAAC  >  CP009273/288102‑288391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑