Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,657,375 G→A 100% P202S (CCT→TCT)  gadW ← transcriptional activator of gadA and gadBC; repressor of gadX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,657,3750GA97.3% 953.4 / 10.3 292P202S (CCT→TCT) gadWtranscriptional activator of gadA and gadBC; repressor of gadX
Reads supporting (aligned to +/- strand):  ref base G (4/4);  new base A (148/136);  total (152/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 300 total aligned reads displayed.

ATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATA  >  CP009273/3657230‑3657519
                                                                                                                                                 |                                                                                                                                                
atatTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGaaa                                                                                                                                                <  2:2139610/148‑1 (MQ=255)
atatTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGaaa                                                                                                                                                <  1:1867595/148‑1 (MQ=255)
 tatTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAAt                                                                                                                                               >  2:2837862/1‑148 (MQ=255)
  atTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAAtt                                                                                                                                              >  2:1412172/1‑148 (MQ=255)
   ttCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAAttt                                                                                                                                             <  1:1018857/148‑1 (MQ=255)
   ttCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAAttt                                                                                                                                             >  2:480514/1‑148 (MQ=255)
   ttCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAAtgt                                                                                                                                             >  2:2339604/1‑146 (MQ=255)
    tCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTg                                                                                                                                            <  2:1485639/148‑1 (MQ=255)
     cAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGATATTTGa                                                                                                                                           <  2:1893235/148‑1 (MQ=255)
       aaTGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACg                                                                                                                                         >  2:132740/1‑148 (MQ=255)
       aaTGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACg                                                                                                                                         >  2:2062326/1‑148 (MQ=255)
       aaTGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACg                                                                                                                                         >  2:1929017/1‑148 (MQ=255)
         tGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTa                                                                                                                                       >  1:724304/1‑148 (MQ=255)
               atatGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGa                                                                                                                                 <  2:1614888/148‑1 (MQ=255)
                tatGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAg                                                                                                                                >  2:2221397/1‑148 (MQ=255)
                  tGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTa                                                                                                                              <  2:2123282/148‑1 (MQ=255)
                        gAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTc                                                                                                                        >  2:3020267/1‑148 (MQ=255)
                        gAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTc                                                                                                                        >  1:881500/1‑148 (MQ=255)
                            aGGTACCTGGCGAAAGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTATTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGAAATCGTCTGGc                                                                                                                    >  2:560612/1‑148 (MQ=255)
                             ggTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGcc                                                                                                                   >  1:286608/1‑148 (MQ=255)
                             ggTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGcc                                                                                                                   >  2:401428/1‑148 (MQ=255)
                               tACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCAt                                                                                                                 <  1:575336/148‑1 (MQ=255)
                                   tGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGAc                                                                                                             <  2:3533650/148‑1 (MQ=255)
                                    ggCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACa                                                                                                            >  2:1015737/1‑148 (MQ=255)
                                       gAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATcc                                                                                                         <  2:3294400/148‑1 (MQ=255)
                                       gAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATcc                                                                                                         <  1:1957976/148‑1 (MQ=255)
                                       gAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATcc                                                                                                         <  2:3598502/148‑1 (MQ=255)
                                               cgcAACCTGCTGTGGCGTTACACCATACTATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCg                                                                                                 <  2:1083517/148‑1 (MQ=255)
                                               cgcAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCg                                                                                                 <  2:3679440/148‑1 (MQ=255)
                                                  aaaCTGATGTGGCGTTACACCATAAGATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGt                                                                                              <  1:3708902/148‑1 (MQ=255)
                                                   aaCTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTa                                                                                             <  1:1371883/148‑1 (MQ=255)
                                                    aCTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTaa                                                                                            >  1:1326070/1‑148 (MQ=255)
                                                     cTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAat                                                                                           >  1:1434491/1‑148 (MQ=255)
                                                       gATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAatat                                                                                         <  1:2062326/148‑1 (MQ=255)
                                                          gtgGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATAtttt                                                                                      >  1:1736283/1‑148 (MQ=255)
                                                          gtgGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATAtttt                                                                                      >  2:1199285/1‑148 (MQ=255)
                                                           tgGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTACGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTa                                                                                     <  1:3609464/148‑1 (MQ=255)
                                                            ggCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTAc                                                                                    >  1:3121095/1‑148 (MQ=255)
                                                            ggCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTAc                                                                                    >  1:1541748/1‑148 (MQ=255)
                                                                 tACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGaaa                                                                               >  1:2178944/1‑148 (MQ=255)
                                                                 tACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGaaa                                                                               >  1:3029899/1‑148 (MQ=255)
                                                                 tACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGaaa                                                                               <  1:963965/148‑1 (MQ=255)
                                                                  acacCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAAc                                                                              <  2:2392306/148‑1 (MQ=255)
                                                                   cacCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACa                                                                             <  1:3537793/148‑1 (MQ=255)
                                                                       ataataTTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTa                                                                         >  2:3429192/1‑148 (MQ=255)
                                                                       ataataTTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTa                                                                         <  1:929908/148‑1 (MQ=255)
                                                                        taataTTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTAt                                                                        <  2:2936111/148‑1 (MQ=255)
                                                                         aataTTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTAtt                                                                       <  2:1271266/148‑1 (MQ=255)
                                                                            atTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTc                                                                    >  2:1137148/1‑148 (MQ=255)
                                                                             ttGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCa                                                                   >  1:257930/1‑148 (MQ=255)
                                                                              tGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCAt                                                                  >  1:2099993/1‑148 (MQ=255)
                                                                                tCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATcc                                                                <  2:2551667/148‑1 (MQ=255)
                                                                                tCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATcc                                                                >  1:643935/1‑148 (MQ=255)
                                                                                 cGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCt                                                               >  1:3047298/1‑148 (MQ=255)
                                                                                    aaTGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCa                                                            <  1:215531/148‑1 (MQ=255)
                                                                                     aTGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCaa                                                           >  1:1729040/1‑148 (MQ=255)
                                                                                     aTGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCaa                                                           >  2:2307823/1‑148 (MQ=255)
                                                                                      tgtgTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAAc                                                          >  2:3032218/1‑148 (MQ=255)
                                                                                      tgtgTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAAc                                                          >  2:2048144/1‑148 (MQ=255)
                                                                                      tgtgTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAAc                                                          >  1:1931025/1‑148 (MQ=255)
                                                                                         gtTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACttt                                                       >  1:2521775/1‑148 (MQ=255)
                                                                                          tttATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACtttt                                                      <  2:3389845/148‑1 (MQ=255)
                                                                                            tataAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACtttttt                                                    <  1:2425231/148‑1 (MQ=255)
                                                                                            tataAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACtttttt                                                    <  2:2198334/148‑1 (MQ=255)
                                                                                            tataAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACtttttt                                                    <  2:1226350/148‑1 (MQ=255)
                                                                                              taAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACtttttttt                                                  <  2:707279/148‑1 (MQ=255)
                                                                                               aaaGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTg                                                 >  1:2987669/1‑148 (MQ=255)
                                                                                               aaaGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTg                                                 >  1:1090728/1‑148 (MQ=255)
                                                                                                aaGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGa                                                <  1:3207354/148‑1 (MQ=255)
                                                                                                 aGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTACTATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTGTTTTgtt                                               >  2:3739445/1‑146 (MQ=255)
                                                                                                    aCGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAg                                            >  1:1068733/1‑148 (MQ=255)
                                                                                                       aTGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGAct                                         <  2:2907559/148‑1 (MQ=255)
                                                                                                           aCTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCg                                     <  2:3199974/148‑1 (MQ=255)
                                                                                                           aCTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACCGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCg                                     >  1:1349157/1‑148 (MQ=255)
                                                                                                             tGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCt                                   >  1:1182201/1‑148 (MQ=255)
                                                                                                                 ataGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTATACTCTCGCTGGta                               >  2:2527287/1‑148 (MQ=255)
                                                                                                                  taGCCACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGtat                              <  1:480514/148‑1 (MQ=255)
                                                                                                                     ccACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACa                           <  1:1839710/148‑1 (MQ=255)
                                                                                                                     ccACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACa                           <  1:1137148/148‑1 (MQ=255)
                                                                                                                     ccACATTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACa                           >  2:2991721/1‑148 (MQ=255)
                                                                                                                      cacaTTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACAt                          <  2:2483821/148‑1 (MQ=255)
                                                                                                                       acaTTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACAtt                         >  2:1946888/1‑148 (MQ=255)
                                                                                                                       acaTTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACAtt                         >  1:3371511/1‑148 (MQ=255)
                                                                                                                       acaTTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACAtt                         >  2:14540/1‑148 (MQ=255)
                                                                                                                        caTTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTc                        <  1:2221348/148‑1 (MQ=255)
                                                                                                                        caTTTTTCATAAATATTATGCATATAAATTTGACTTAACTCGAGTAATCGTCTGGCCATCTACTTCATTGAGGCGATTAATATTTTTCTGATATAGTTATTTTAATCCTTAAAGTTTTTTTTGTTTATACTCTATCTGGTATACttta                        <  2:3560734/148‑2 (MQ=255)
                                                                                                                         aTTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCt                       <  1:1290505/148‑1 (MQ=255)
                                                                                                                         aTTTTTCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCt                       <  1:167745/148‑1 (MQ=255)
                                                                                                                          tttttCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCtt                      >  1:1831813/1‑148 (MQ=255)
                                                                                                                           ttttCCGCAATAGTATGCAGAGAAATTTGATGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCttt                     <  2:2689619/148‑1 (MQ=255)
                                                                                                                           ttttCCGCAATAGGATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCttt                     >  1:2769183/1‑148 (MQ=255)
                                                                                                                            tttCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTc                    <  2:841167/148‑1 (MQ=255)
                                                                                                                             ttCCGCAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTcc                   >  1:3307734/1‑148 (MQ=255)
                                                                                                                                  cAATAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGat              >  1:2233304/1‑148 (MQ=255)
                                                                                                                                     tAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATc           >  2:1830213/1‑148 (MQ=255)
                                                                                                                                     tAGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATc           >  1:437961/1‑148 (MQ=255)
                                                                                                                                      aGTATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATcg          >  1:1806694/1‑148 (MQ=255)
                                                                                                                                        tATGCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATcgcg        >  1:3469021/1‑148 (MQ=255)
                                                                                                                                           gCAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAg     <  1:1380406/148‑1 (MQ=255)
                                                                                                                                            cAGAGAAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGa    <  2:3095127/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATATTCAAATGCGAAATATGTCAGGAAAAGGTACCTGGCGAATGTTGCGCAAACTGATGTGGCGTTACACCATAATATTGTCGAAATGTGTTTATAAAGTACGATGTACTGCTATAGCCACATTTTTCCGCAATAGTATGCAGAGGAATTTGACGTAACTCGAGTAATCGTCTGGCCATCGACATCCTGGAGGCGAGTAATATTTTACTGAAACAGGTATTTTCATCCTGCAACTTTTTTTTGATTAGACTCTCGCTGGTATACATTCTTTCCGCGATATCGCGCAGATA  >  CP009273/3657230‑3657519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑