Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,686,345 C→A 100% R751L (CGC→CTC)  bcsA ← cellulose synthase, catalytic subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,686,3450CA99.4% 555.9 / ‑4.2 169R751L (CGC→CTC) bcsAcellulose synthase, catalytic subunit
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (77/91);  total (78/91)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 169 total aligned reads displayed.

CCAGAGCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCGGGCATCGTC  >  CP009273/3686200‑3686488
                                                                                                                                                 |                                                                                                                                               
ccAGAGCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGt                                                                                                                                               <  1:1651488/148‑1 (MQ=255)
  agagCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGttt                                                                                                                                             >  1:2937740/1‑148 (MQ=255)
  agagCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGttt                                                                                                                                             >  1:3121847/1‑148 (MQ=255)
  agagCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGttt                                                                                                                                             >  1:3122015/1‑148 (MQ=255)
   gagCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTa                                                                                                                                            <  1:3322649/148‑1 (MQ=255)
   gagCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTa                                                                                                                                            <  2:3811324/148‑1 (MQ=255)
   gagCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTa                                                                                                                                            <  2:2996784/148‑1 (MQ=255)
    agCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTaa                                                                                                                                           >  1:916132/1‑148 (MQ=255)
    agCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTaa                                                                                                                                           <  2:3731443/148‑1 (MQ=255)
     gcgcCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAg                                                                                                                                          <  2:539641/148‑1 (MQ=255)
         ccATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAAc                                                                                                                                      <  2:3622594/148‑1 (MQ=255)
           aTGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACACATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAg                                                                                                                                    >  2:3156132/1‑148 (MQ=255)
            tGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGa                                                                                                                                   <  1:2175441/148‑1 (MQ=255)
             ggaTCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGAt                                                                                                                                  <  2:3778488/146‑1 (MQ=255)
              tATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGAtt                                                                                                                                 <  1:532404/148‑1 (MQ=255)
              tATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGAtt                                                                                                                                 <  1:3776276/148‑1 (MQ=255)
              tATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGAtt                                                                                                                                 <  1:1042681/148‑1 (MQ=255)
               aTCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTACGCAACAGATTc                                                                                                                                <  1:3124/148‑1 (MQ=255)
                tCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCa                                                                                                                               <  1:1013077/148‑1 (MQ=255)
                 ccGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCAc                                                                                                                              >  1:1789854/1‑148 (MQ=255)
                 ccGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCAc                                                                                                                              <  1:273409/148‑1 (MQ=255)
                    cACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACttt                                                                                                                           >  2:576375/1‑148 (MQ=255)
                    cACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACttt                                                                                                                           <  1:2542769/148‑1 (MQ=255)
                     aCGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTc                                                                                                                          <  1:586657/148‑1 (MQ=255)
                      cGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCt                                                                                                                         <  2:1759346/148‑1 (MQ=255)
                         gCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGcc                                                                                                                      <  2:3653286/148‑1 (MQ=255)
                         gCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGcc                                                                                                                      <  1:3407086/148‑1 (MQ=255)
                          cAAACGTACACTGCACATAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGccc                                                                                                                     <  2:3639883/148‑1 (MQ=255)
                             aCGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTc                                                                                                                  <  2:3373120/148‑1 (MQ=255)
                              cGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTcc                                                                                                                 >  2:983919/1‑148 (MQ=255)
                                 acacTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGa                                                                                                              >  1:2527962/1‑148 (MQ=255)
                                  cacTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGaa                                                                                                             <  2:262129/148‑1 (MQ=255)
                                  cacTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGaa                                                                                                             >  1:3028049/1‑148 (MQ=255)
                                   acTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAAt                                                                                                            >  2:1424983/1‑148 (MQ=255)
                                    cTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATc                                                                                                           >  1:2403463/1‑148 (MQ=255)
                                    cTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATc                                                                                                           <  2:1724435/148‑1 (MQ=255)
                                         caAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAATCAACAGATTCACTTTCTGCCCTTCCAGAATCTgcgc                                                                                                      >  1:12183/1‑148 (MQ=255)
                                             aTCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGa                                                                                                  <  1:2327282/148‑1 (MQ=255)
                                               cGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCACCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGAcc                                                                                                >  1:907891/1‑148 (MQ=255)
                                                  tatGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGtt                                                                                             >  2:3107223/1‑148 (MQ=255)
                                                  tatGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGtt                                                                                             >  2:2389271/1‑148 (MQ=255)
                                                  tatGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGtt                                                                                             >  2:1201025/1‑148 (MQ=255)
                                                   atGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttg                                                                                            <  2:2918749/148‑1 (MQ=255)
                                                     gTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttgat                                                                                          <  1:2450542/148‑1 (MQ=255)
                                                     gTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttgat                                                                                          <  1:1424983/148‑1 (MQ=255)
                                                      ttGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttgatc                                                                                         >  2:3286264/1‑148 (MQ=255)
                                                      ttGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttgatc                                                                                         >  1:2151054/1‑148 (MQ=255)
                                                      ttGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttgatc                                                                                         <  1:241737/148‑1 (MQ=255)
                                                      ttGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttgatc                                                                                         <  1:2171816/148‑1 (MQ=255)
                                                       tGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttgatct                                                                                        <  2:2361884/148‑1 (MQ=255)
                                                       tGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttgatct                                                                                        <  2:1607868/148‑1 (MQ=255)
                                                         cTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttgatcttg                                                                                      <  1:135944/148‑1 (MQ=255)
                                                          tGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttgatcttga                                                                                     <  1:3068254/148‑1 (MQ=255)
                                                           gggTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttgatcttgat                                                                                    >  2:1094788/1‑148 (MQ=255)
                                                           gggTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTANTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttgatcttgat                                                                                    >  1:1932336/1‑148 (MQ=255)
                                                            ggtggtGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttgatcttgatc                                                                                   >  1:2124219/1‑148 (MQ=255)
                                                            ggtggtGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGttgatcttgatc                                                                                   <  2:825946/148‑1 (MQ=255)
                                                               ggtgAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATcccc                                                                                >  2:3684600/1‑148 (MQ=255)
                                                                  gAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCaaa                                                                             <  2:2648643/148‑1 (MQ=255)
                                                                  gAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCaaa                                                                             <  2:1729179/148‑1 (MQ=255)
                                                                  gAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCaaa                                                                             <  2:2649075/148‑1 (MQ=255)
                                                                           tAATTTCAGACCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCg                                                                    >  2:2638744/1‑148 (MQ=255)
                                                                             aTTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGga                                                                  >  1:1058014/1‑148 (MQ=255)
                                                                                   gCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATc                                                            >  1:817932/1‑148 (MQ=255)
                                                                                   gCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATc                                                            >  2:3452276/1‑148 (MQ=255)
                                                                                       aaCTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGa                                                        <  1:458716/148‑1 (MQ=255)
                                                                                            cATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGt                                                   >  1:3791813/1‑148 (MQ=255)
                                                                                            cATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGt                                                   >  1:241928/1‑148 (MQ=255)
                                                                                              ttACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTac                                                 <  1:3119815/148‑1 (MQ=255)
                                                                                               tACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTaca                                                <  1:3458411/148‑1 (MQ=255)
                                                                                                   cATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACgag                                            <  1:3259443/148‑1 (MQ=255)
                                                                                                   cATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACgag                                            <  1:3260262/148‑1 (MQ=255)
                                                                                                     tCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGagaa                                          >  1:2263179/1‑148 (MQ=255)
                                                                                                      cACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGagaag                                         <  2:183598/148‑1 (MQ=255)
                                                                                                       aCGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGagaaga                                        >  2:2061354/1‑148 (MQ=255)
                                                                                                       aCGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGagaaga                                        >  2:2061453/1‑148 (MQ=255)
                                                                                                        cgcgcgCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAagag                                       <  2:1650724/148‑1 (MQ=255)
                                                                                                           gcgcCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTg                                    <  1:1004489/148‑1 (MQ=255)
                                                                                                                aCCTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCAt                               <  1:530036/148‑1 (MQ=255)
                                                                                                                 ccTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATc                              >  1:2061740/1‑148 (MQ=255)
                                                                                                                 ccTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATc                              >  1:2061744/1‑148 (MQ=255)
                                                                                                                 ccTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATc                              >  1:34138/1‑148 (MQ=255)
                                                                                                                 ccTGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTGTAGATTGATCCCCAAACAACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATc                              <  1:3745928/148‑1 (MQ=255)
                                                                                                                   tGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCtt                            <  1:530703/148‑1 (MQ=255)
                                                                                                                   tGGGTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCtt                            >  2:2571611/1‑148 (MQ=255)
                                                                                                                     ggTCGGGAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTcg                          >  2:1201381/1‑148 (MQ=255)
                                                                                                                          ggAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGc                     >  2:2424438/1‑148 (MQ=255)
                                                                                                                          ggAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGc                     >  2:953491/1‑148 (MQ=255)
                                                                                                                           gAAGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCa                    <  1:426295/148‑1 (MQ=255)
                                                                                                                            aaGACGTATTCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCaa                   >  2:2486157/1‑148 (MQ=255)
                                                                                                                                    ttCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCcgcg           <  2:2124219/148‑1 (MQ=255)
                                                                                                                                     tCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCgg          >  2:874361/1‑148 (MQ=255)
                                                                                                                                     tCCTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCgg          >  1:1781581/1‑148 (MQ=255)
                                                                                                                                      ccTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCggg         >  1:742467/1‑148 (MQ=255)
                                                                                                                                      ccTGCTGACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCggg         >  1:681477/1‑148 (MQ=255)
                                                                                                                                        tgctgACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCGGGcc       >  1:2330410/1‑147 (MQ=255)
                                                                                                                                        tgctgACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCGGGCa       >  1:571274/1‑148 (MQ=255)
                                                                                                                                         gctgACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCACTTGCCGCGGGCAt      >  2:862315/1‑148 (MQ=255)
                                                                                                                                            gACCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCGGGCATCGt   >  1:1018594/1‑148 (MQ=255)
                                                                                                                                             aCCGAGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCGGGCATCGTc  >  1:974127/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CCAGAGCGCCCATGTATCCGCACGGGCAAACGTACACTGCACAAAATCGATATGTTGCTGGGTGGTGAGCGGCATTAATTTCAGCCCAACTTCATTACCCATCACGCGCGCCACCTGGGTCGGGAAGACGTATTCCTGCTGACCGCGTTTAAGCAACAGATTCACTTTCTGCCCTTCCAGAATCTGCGCCTGACCGTTGATCTTGATCCCCAAACCACCGTCGGAGAAATCCTGAACGGTACACGAGAAGAGGTGACCATCTTCGCGGGCAATTGCCGCGGGCATCGTC  >  CP009273/3686200‑3686488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑