Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,692,994 T→A 100% F474Y (TTC→TAC)  bcsG → DUF3260 family inner membrane protein associated with cellulose production

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,692,9940TA99.4% 580.1 / ‑4.5 175F474Y (TTC→TAC) bcsGDUF3260 family inner membrane protein associated with cellulose production
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (95/79);  total (95/80)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 182 total aligned reads displayed.

TTACTGAACTTGAGAAATCGGGTCGTAAAGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATTCTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCGTTCTCGATGGCAAGATTTTCACCGAAGACAATGTTGACTGGAAAAAACTCACC  >  CP009273/3692847‑3693136
                                                                                                                                                   |                                                                                                                                              
ttACTGAACTTGAGAAATCGGGTCGTAAAGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAAtt                                                                                                                                                >  2:376847/1‑148 (MQ=255)
  aCTGAACTTGAGAAATCGGGTCGTAAAGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAAtact                                                                                                                                              <  2:367136/148‑1 (MQ=255)
  aCTGAACTTGAGAAATCGGGTCGTAAAGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAAtact                                                                                                                                              >  1:1809515/1‑148 (MQ=255)
  aCTGAACTTGAGAAATCGGGTCGTAAAGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAAtact                                                                                                                                              >  1:1809131/1‑148 (MQ=255)
   cTGAACTTGAGAAATCGGGTCGTAAAGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAAtactt                                                                                                                                             <  2:512420/148‑1 (MQ=255)
      aaCTTGAGAAATCGGGTCGTAAAGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGATGGGGTGAAATACTTCgg                                                                                                                                          >  1:2457655/1‑148 (MQ=255)
          tGAGAAATCGGGTCGTAAAGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGGTGGGGTGAAATACTTCGGCATg                                                                                                                                      >  2:3766417/1‑148 (MQ=255)
           gagaAATCGGGTCGTAAAGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGa                                                                                                                                     <  2:3497917/148‑1 (MQ=255)
            agaAATCGGGTCGTAAAGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGaa                                                                                                                                    <  1:837894/148‑1 (MQ=255)
            agaAATCGGGTCGTAAAGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGaa                                                                                                                                    <  1:837869/148‑1 (MQ=255)
              aaaTCGGGTCGTAAAGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAgg                                                                                                                                  <  2:781399/148‑1 (MQ=255)
               aaTCGGGTCGTAAAGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGc                                                                                                                                 <  1:3052191/148‑1 (MQ=255)
               aaTCGGGTCGTAAAGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGc                                                                                                                                 <  1:3021441/148‑1 (MQ=255)
                aTCGGGTCGTAAAGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCa                                                                                                                                >  1:1091775/1‑148 (MQ=255)
                 tCGGGTCGTAAAGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCAc                                                                                                                               >  2:3129484/1‑148 (MQ=255)
                     gTCGTAAAGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCa                                                                                                                           <  2:2383063/148‑1 (MQ=255)
                      tCGTAAAGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCAt                                                                                                                          >  1:3176806/1‑148 (MQ=255)
                       cGTAAAGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCCCCGCATc                                                                                                                         <  1:2155242/148‑1 (MQ=255)
                       cGTAAAGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATc                                                                                                                         >  1:926217/1‑148 (MQ=255)
                          aaaGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAgg                                                                                                                      <  1:1397464/148‑1 (MQ=255)
                          aaaGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAgg                                                                                                                      >  1:628437/1‑148 (MQ=255)
                          aaaGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAgg                                                                                                                      >  1:1015344/1‑148 (MQ=255)
                          aaaGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAgg                                                                                                                      >  1:3785929/1‑148 (MQ=255)
                             gTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAggggg                                                                                                                   <  1:688733/148‑1 (MQ=255)
                               gATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCa                                                                                                                 >  1:1344863/1‑148 (MQ=255)
                                aTGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCAc                                                                                                                >  1:1542351/1‑148 (MQ=255)
                                   gtggtCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGa                                                                                                             >  2:3267882/1‑148 (MQ=255)
                                    tggtCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGAt                                                                                                            <  1:3621223/148‑1 (MQ=255)
                                      gtCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTg                                                                                                          >  2:525219/1‑148 (MQ=255)
                                       tCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGt                                                                                                         <  2:1500524/148‑1 (MQ=255)
                                       tCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGt                                                                                                         >  1:191468/1‑148 (MQ=255)
                                        cGTGGTGCCGGAACACGGCGGCGCGGTGTTGCCCTACAGAAAGAAGGTATCTGGGCTGCCTGGAATCCCTAGCCCGTCTATCAGCGTCGACCGCGTTTGGTTGTAATACTTCGGCATGTAGGCACCGCATCAGGGGGCACGGGTTGTc                                                                                                        >  2:561801/1‑148 (MQ=255)
                                         gtggtgCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCa                                                                                                       <  1:311989/148‑1 (MQ=255)
                                         gtggtgCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCa                                                                                                       <  1:3388200/148‑1 (MQ=255)
                                         gtggtgCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCa                                                                                                       <  1:1486481/148‑1 (MQ=255)
                                          tggtgCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCAt                                                                                                      <  1:607553/148‑1 (MQ=255)
                                          tggtgCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGGTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCAt                                                                                                      >  2:3632322/1‑148 (MQ=255)
                                           ggtgCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATc                                                                                                     >  2:194512/1‑148 (MQ=255)
                                             tgCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGa                                                                                                   >  2:2183676/1‑148 (MQ=255)
                                             tgCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGa                                                                                                   <  2:2879338/148‑1 (MQ=255)
                                                cGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGaaca                                                                                                <  2:1267186/148‑1 (MQ=255)
                                                  gAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGaacaac                                                                                              >  2:1769962/1‑148 (MQ=255)
                                                     cacGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGa                                                                                           >  1:432863/1‑148 (MQ=255)
                                                     cacGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGa                                                                                           >  2:3468743/1‑148 (MQ=255)
                                                      acGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGag                                                                                          <  1:2460872/148‑1 (MQ=255)
                                                       cggcggCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGagc                                                                                         >  2:2287734/1‑148 (MQ=255)
                                                       cggcggCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGagc                                                                                         <  1:2924660/148‑1 (MQ=255)
                                                         gcggcgCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGagcag                                                                                       <  1:470174/148‑1 (MQ=255)
                                                             cgcgcTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTc                                                                                   <  2:112000/148‑1 (MQ=255)
                                                                     aGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTAt                                                                           >  1:793883/1‑148 (MQ=255)
                                                                       ggCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTAtct                                                                         <  2:1724884/148‑1 (MQ=255)
                                                                        gCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTAtctc                                                                        <  2:3744353/148‑1 (MQ=255)
                                                                          gACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCg                                                                      <  2:1232648/148‑1 (MQ=255)
                                                                           aCAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTGGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCGGGCTATCTCCGa                                                                     >  1:1850384/1‑148 (MQ=255)
                                                                               aaTGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCtg                                                                 >  1:2678637/1‑148 (MQ=255)
                                                                               aaTGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCtg                                                                 >  2:2717026/1‑148 (MQ=255)
                                                                                   cAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCtggtgg                                                             <  1:1804564/148‑1 (MQ=255)
                                                                                       tATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGTCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTTGTTcg                                                         >  1:3143070/1‑148 (MQ=255)
                                                                                        aTCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTcgc                                                        >  2:3018367/1‑148 (MQ=255)
                                                                                        aTCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTcgc                                                        <  2:1842580/148‑1 (MQ=255)
                                                                                         tatGGACTACGTGATAACCCTAGCCCGTCTATCACCGAAGTCCAAGTTGGGGTGAAATAATTCGGCATGAAGGAACCGCATCAGGGGGCACCGATTGTCATCGAACAACAGAGCAGCTTCCTGGCTATCTCCGATCTGTTGGTTcgat                                                       >  2:1333412/3‑146 (MQ=255)
                                                                                         tCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTcgcg                                                       <  2:3250711/148‑1 (MQ=255)
                                                                                          cTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCGt                                                      >  2:3180733/1‑148 (MQ=255)
                                                                                          cTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCGt                                                      >  2:2302968/1‑148 (MQ=255)
                                                                                            ggCCTACGAGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCGTtc                                                    <  1:3467228/148‑1 (MQ=255)
                                                                                             gCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCTTtct                                                   <  1:3783056/148‑1 (MQ=255)
                                                                                               cTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCTTTCTCg                                                 >  1:27949/1‑148 (MQ=255)
                                                                                               cTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCGTTCTCg                                                 <  2:2457471/148‑1 (MQ=255)
                                                                                                tACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCGTTCTCGa                                                >  1:791692/1‑148 (MQ=255)
                                                                                                  cGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCGTTCTCGATg                                              >  2:2137328/1‑148 (MQ=255)
                                                                                                   gTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCGTTCTCGATgg                                             >  1:446639/1‑148 (MQ=255)
                                                                                                          cccTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCGTTCTCGATGGCAAGAtt                                      <  1:594125/148‑1 (MQ=255)
                                                                                                           ccTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCTTTCTCGATGGCAAGAttt                                     >  1:3667521/1‑148 (MQ=255)
                                                                                                           ccTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCTTTCTCGATGGCAAGAttt                                     >  1:1715448/1‑148 (MQ=255)
                                                                                                           ccTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCTTTCTCGATGGCAAGAttt                                     >  1:1716217/1‑148 (MQ=255)
                                                                                                             tAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCGTTCTCGATGGCAAGATTTTc                                   >  1:1577049/1‑148 (MQ=255)
                                                                                                               gCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCGTTCTCGATGGCAAGATTTTCAc                                 <  1:3442880/148‑1 (MQ=255)
                                                                                                                cccGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCTTTCTCGATGGCAAGATTTTCAcc                                >  2:3531994/1‑148 (MQ=255)
                                                                                                                  cGTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCTTTCTCGATGGCAAGATTTTCACCGa                              <  2:1623747/148‑1 (MQ=255)
                                                                                                                   gTCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGACATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCGGTCTCGATGGCAAGATTTTCACCGaa                             >  1:115878/1‑148 (MQ=255)
                                                                                                                    tCTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCGTTCTCGATGGCAAGATTTTCACCGAAg                            <  1:404455/148‑1 (MQ=255)
                                                                                                                     cTATCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTACGATCTGGTGGTTCGCGTTCTCGATGGCAAGATTTTCACCGAAGa                           >  2:1667216/1‑148 (MQ=255)
                                                                                                                       aTCACCGACGTCCCCGTTGGGGTGAAATTCTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCGTTCTCGATGGCAAGATTTTCACCGAAGACa                         <  1:376847/148‑1 (MQ=255)
                                                                                                                       aTCACCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGAGTTCTCGATGGCAAGATTTTCACCGAAGACa                         <  1:3496233/148‑1 (MQ=255)
                                                                                                                          aCCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCGTTCTCGATGGCAAGATTTTCACCGAAGACAATg                      >  2:2618550/1‑148 (MQ=255)
                                                                                                                          aCCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCGTTCTCGATGGCAAGATTTTCACCGAAGACAATg                      >  2:2618545/1‑148 (MQ=255)
                                                                                                                          aCCGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCGTTCTCGATGGCAAGATTTTCACCGAAGACAATg                      <  2:1750423/148‑1 (MQ=255)
                                                                                                                           ccGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCTTTCTCGATGGCAAGATTTTCACCGAAGACAATGt                     >  2:2529209/1‑148 (MQ=255)
                                                                                                                           ccGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCTTTCTCGATGGCAAGATTTTCACCGAAGACAATGt                     <  2:2643408/148‑1 (MQ=255)
                                                                                                                            cGACGTCCCCGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCTTTCTCGATGGCAAGATTTTCACCGAAGACAATGtt                    >  2:1655882/1‑148 (MQ=255)
                                                                                                                                  ccccGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCTTTCTCGATGGCAAGATTTTCACCGAAGACAATGTTGACTgg              >  1:2332004/1‑148 (MQ=255)
                                                                                                                                   cccGTTGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCTTTCTCGATGGCAAGATTTTCACCGAAGACAATGTTGACTGGa             >  2:1666873/1‑148 (MQ=255)
                                                                                                                                       ttGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCTTTCTCGATGGCAAGATTTTCACCGAAGACAATGTTGACTGGaaaaa         >  1:3350949/1‑148 (MQ=255)
                                                                                                                                       ttGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCTTTCTCGATGGCAAGATTTTCACCGAAGACAATGTTGACTGGaaaaa         >  1:2665518/1‑148 (MQ=255)
                                                                                                                                       ttGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCGTTCTCGATGGCAAGATTTTCACCGAAGACAATGTTGACTGGaaaaa         >  2:2918898/1‑148 (MQ=255)
                                                                                                                                       ttGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCGTTCTCGATGGCAAGATTTTCACCGAAGACAATGTTGACTGGaaaaa         >  2:1256031/1‑148 (MQ=255)
                                                                                                                                        tGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCTTTCTCGATGGCAAGATTTTCACCGAAGACAATGTTGACTGGaaaaaa        <  2:1015344/148‑1 (MQ=255)
                                                                                                                                        tGGGGTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCTTTCTCGATGGCAAGATTTTCACCGAAGACAATGTTGACTGGaaaaaa        <  2:1788037/148‑1 (MQ=255)
                                                                                                                                            gTGAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATAGAACAACCGAGAAGCTTCCTGGCTATATCCGATCTGGTGGTTCGCGTTCTCGATGGCAAGATTTTCACCGAAGACAATGTTGACTGGAAAAAAATCa    >  1:2005628/1‑148 (MQ=255)
                                                                                                                                              gAAATACTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCTTTCTCGATGGCAAGATTTTCACCGAAGACAATGTTGACTGGAAAAAACTCAcc  <  1:1769962/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
TTACTGAACTTGAGAAATCGGGTCGTAAAGTGATGGTGGTCGTGGTGCCGGAACACGGCGGCGCGCTGAAGGGCGACAGAATGCAGGTATCTGGCCTACGTGATATCCCTAGCCCGTCTATCACCGACGTCCCCGTTGGGGTGAAATTCTTCGGCATGAAGGCACCGCATCAGGGGGCACCGATTGTCATCGAACAACCGAGCAGCTTCCTGGCTATCTCCGATCTGGTGGTTCGCGTTCTCGATGGCAAGATTTTCACCGAAGACAATGTTGACTGGAAAAAACTCACC  >  CP009273/3692847‑3693136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑