Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,717,788 C→A 100% P297P (CCG→CCT)  glyQ ← glycine tRNA synthetase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,717,7880CA99.5% 697.4 / ‑4.6 211P297P (CCG→CCT) glyQglycine tRNA synthetase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (109/101);  minor base T (0/1);  total (109/102)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 211 total aligned reads displayed.

CAGCGTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTC  >  CP009273/3717643‑3717932
                                                                                                                                                 |                                                                                                                                                
 aGCGTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAggg                                                                                                                                               <  2:1141940/148‑1 (MQ=255)
  gCGTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGa                                                                                                                                              <  2:770490/148‑1 (MQ=255)
  gCGTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGa                                                                                                                                              <  2:3282858/148‑1 (MQ=255)
  gCGTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGa                                                                                                                                              <  2:1568744/148‑1 (MQ=255)
   cGTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGaa                                                                                                                                             >  1:364794/1‑148 (MQ=255)
    gTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAg                                                                                                                                            <  2:906909/148‑1 (MQ=255)
    gTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAg                                                                                                                                            <  2:1195775/148‑1 (MQ=255)
    gTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAg                                                                                                                                            <  2:1775436/148‑1 (MQ=255)
    gTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAg                                                                                                                                            <  2:3132865/148‑1 (MQ=255)
        tCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGa                                                                                                                                        >  2:3024791/1‑148 (MQ=255)
        tCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGa                                                                                                                                        <  1:2015670/148‑1 (MQ=255)
            gCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCTCATAGGGAAGCCGAGGGc                                                                                                                                    >  2:258010/1‑148 (MQ=255)
            gCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGc                                                                                                                                    >  1:2934742/1‑148 (MQ=255)
            gCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGc                                                                                                                                    >  2:3177150/1‑148 (MQ=255)
               ccGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTc                                                                                                                                 >  2:3320207/1‑148 (MQ=255)
                      aaaGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGaa                                                                                                                          >  1:3125310/1‑148 (MQ=255)
                       aaGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAg                                                                                                                         <  2:5402/148‑1 (MQ=255)
                          ttCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCgt                                                                                                                      >  1:3614248/1‑148 (MQ=255)
                          ttCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCgt                                                                                                                      <  2:3239354/148‑1 (MQ=255)
                             gcagcaAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCgtagt                                                                                                                   <  2:2487167/148‑1 (MQ=255)
                             gcagcaAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCgtagt                                                                                                                   <  1:2252096/148‑1 (MQ=255)
                                gcaAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATg                                                                                                                >  2:1986522/1‑148 (MQ=255)
                                gcaAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATg                                                                                                                <  2:1824190/148‑1 (MQ=255)
                                gcaAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATg                                                                                                                <  2:1772548/148‑1 (MQ=255)
                                gcaAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATg                                                                                                                >  1:731421/1‑148 (MQ=255)
                                 caAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGc                                                                                                               <  1:2026463/148‑1 (MQ=255)
                                 caAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGc                                                                                                               >  1:115462/1‑148 (MQ=255)
                                   aaGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCtt                                                                                                             <  1:1900083/148‑1 (MQ=255)
                                     ggACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCt                                                                                                           >  2:2268796/1‑148 (MQ=255)
                                        cTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGcc                                                                                                        <  1:1846891/148‑1 (MQ=255)
                                            gCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTg                                                                                                    <  2:1861786/148‑1 (MQ=255)
                                               aGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCtt                                                                                                 >  1:1065910/1‑148 (MQ=255)
                                                 gCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTg                                                                                               >  1:2751054/1‑148 (MQ=255)
                                                 gCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTg                                                                                               >  2:2415269/1‑148 (MQ=255)
                                                 gCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTg                                                                                               >  1:1352311/1‑148 (MQ=255)
                                                    gcgcAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGACGCGTAGTATGCTTCTGCCACTGCTTTGGTc                                                                                            >  2:2918392/1‑148 (MQ=255)
                                                       cAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAgg                                                                                         <  2:3121859/148‑1 (MQ=255)
                                                          tGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTg                                                                                      <  1:2959898/148‑1 (MQ=255)
                                                             ttttGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGa                                                                                   <  1:2240287/148‑1 (MQ=255)
                                                              tttGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGaa                                                                                  >  1:2502464/1‑148 (MQ=255)
                                                                         ctctTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATg                                                                       >  2:1017909/1‑148 (MQ=255)
                                                                          tctTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGt                                                                      >  2:285726/1‑148 (MQ=255)
                                                                           ctTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACCTAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTa                                                                     >  1:1678926/1‑148 (MQ=255)
                                                                              cAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAgcg                                                                  <  2:3387066/148‑1 (MQ=255)
                                                                              cAGTGCCGATTTCCACAAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAgcg                                                                  <  1:3702351/148‑1 (MQ=255)
                                                                                 tGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTg                                                               >  2:1273928/1‑148 (MQ=255)
                                                                                    cGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACg                                                            <  1:630957/148‑1 (MQ=255)
                                                                                        ttCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCg                                                        >  1:930609/1‑148 (MQ=255)
                                                                                        ttCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCg                                                        >  1:385976/1‑148 (MQ=255)
                                                                                        ttCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCg                                                        >  2:2138148/1‑148 (MQ=255)
                                                                                          ccaccaGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGt                                                      <  1:871002/148‑1 (MQ=255)
                                                                                           ctccaTAAAAGTTTTCTCAGTCTTTGCGGAGTGTTACTTATCTTTGTTGCACTTTGGGAAGACGATGGCTTCACGGGTTGCGTAGTTTGCTTGTGGCTATGGTTTGGTGTGGGTGGGTTTTCGAATTATGTATCGCTTACTGTCGGTg                                                     <  2:1124128/146‑1 (MQ=255)
                                                                                           caccaGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTg                                                     <  2:2391728/148‑1 (MQ=255)
                                                                                            accaGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGa                                                    >  1:2526586/1‑148 (MQ=255)
                                                                                             ccaGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGAc                                                   >  1:833477/1‑148 (MQ=255)
                                                                                                  aaaGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGgaga                                              >  1:2482438/1‑148 (MQ=255)
                                                                                                   aaGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGACGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGAt                                             >  2:2744026/1‑148 (MQ=255)
                                                                                                   aaGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGAt                                             <  2:2966737/148‑1 (MQ=255)
                                                                                                     gTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATgg                                           >  1:1201856/1‑148 (MQ=255)
                                                                                                     gTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATgg                                           <  1:144834/148‑1 (MQ=255)
                                                                                                      ttttCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCTCGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGc                                          <  2:1283936/148‑1 (MQ=255)
                                                                                                      ttttCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGc                                          <  2:1478284/148‑1 (MQ=255)
                                                                                                      ttttCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGc                                          <  2:2824743/148‑1 (MQ=255)
                                                                                                        ttCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCtt                                        <  1:2585687/148‑1 (MQ=255)
                                                                                                        ttCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCtt                                        <  1:2634201/148‑1 (MQ=255)
                                                                                                         tctcAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCttt                                       <  2:1834089/148‑1 (MQ=255)
                                                                                                         tctcAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCttt                                       <  1:3177150/148‑1 (MQ=255)
                                                                                                         tctcAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCttt                                       >  2:1060925/1‑148 (MQ=255)
                                                                                                               aCATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACgcgc                                 >  1:2610533/1‑148 (MQ=255)
                                                                                                                cATAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCa                                >  1:154959/1‑148 (MQ=255)
                                                                                                                 aTAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCAt                               >  2:2490397/1‑148 (MQ=255)
                                                                                                                 aTAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCAt                               <  2:3689515/148‑1 (MQ=255)
                                                                                                                  tAGCCGCCTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATc                              >  1:3422024/1‑148 (MQ=255)
                                                                                                                      cgccTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCcagc                          <  1:103426/148‑1 (MQ=255)
                                                                                                                      cgccTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCcagc                          <  1:4876/148‑1 (MQ=255)
                                                                                                                       gccTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCcagca                         <  1:1298457/148‑1 (MQ=255)
                                                                                                                       gccTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCcagca                         >  2:3235849/1‑148 (MQ=255)
                                                                                                                        ccTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCcagcag                        >  2:1954458/1‑148 (MQ=255)
                                                                                                                        ccTCTTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCcagcag                        <  1:2840425/148‑1 (MQ=255)
                                                                                                                         ctctTACTTATCTTTGTTGCACATAGTGAAGCCGAGGGCTTCACGGGAAGCGTAGTATTCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAgg                       <  1:3556831/148‑1 (MQ=255)
                                                                                                                         ctctTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAgg                       >  2:2895513/1‑148 (MQ=255)
                                                                                                                         ctctTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAgg                       <  1:349400/148‑1 (MQ=255)
                                                                                                                         ctctTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAgg                       >  2:2895745/1‑148 (MQ=255)
                                                                                                                          tctTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGt                      >  2:470167/1‑148 (MQ=255)
                                                                                                                          tctTACTTATCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGt                      >  2:472017/1‑148 (MQ=255)
                                                                                                                             tacttaTCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGa                   <  1:1221971/148‑1 (MQ=255)
                                                                                                                              acttaTCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGaa                  >  1:762409/1‑148 (MQ=255)
                                                                                                                               cttaTCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAg                 <  1:3380346/148‑1 (MQ=255)
                                                                                                                               cttaTCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAg                 <  2:3466894/148‑1 (MQ=255)
                                                                                                                                ttaTCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGc                >  2:1250124/1‑148 (MQ=255)
                                                                                                                                ttaTCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGc                >  2:718128/1‑148 (MQ=255)
                                                                                                                                 taTCTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCt               >  2:3815865/1‑148 (MQ=255)
                                                                                                                                    cTTTGTTGCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCtggg            >  2:880745/1‑146 (MQ=255)
                                                                                                                                        gttgCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGggcg        <  2:3382039/148‑1 (MQ=255)
                                                                                                                                         ttgCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGggcgg       >  2:3495627/1‑148 (MQ=255)
                                                                                                                                          tgCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGggcggc      >  2:2502678/1‑148 (MQ=255)
                                                                                                                                           gCACATAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCt     <  1:3794538/148‑1 (MQ=255)
                                                                                                                                            cacaTAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCtt    <  2:3634266/148‑1 (MQ=255)
                                                                                                                                             acaTAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCttt   <  1:3265295/148‑1 (MQ=255)
                                                                                                                                              caTAGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTc  >  2:2634838/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CAGCGTTATCCAGCTCCGCAGTAAAGTTCGCAGCAAAGGACTCAGCCAGGCTGCGCAGTGCTTTTGGTGGCAGCTCTTCAGTGCCGATTTCCACCAGAAAAGTTTTCTCAGACATAGCCGCCTCTTACTTATCTTTGTTGCACATCGGGAAGCCGAGGGCTTCACGGGAAGCGTAGTATGCTTCTGCCACTGCTTTGGTCAGGGTGCGAATGCGCAGAATGTAGCGCTGACGCTCGGTGACGGAGATGGCTTTACGCGCATCCAGCAGGTTGAAGCTGTGGGCGGCTTTC  >  CP009273/3717643‑3717932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑