Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,767,410 G→T 100% V590V (GTG→GTT)  mtlA → fused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,767,4100GT95.8% 582.7 / 14.9 192V590V (GTG→GTT) mtlAfused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components
Reads supporting (aligned to +/- strand):  ref base G (4/4);  new base T (107/77);  total (111/81)
Fisher's exact test for biased strand distribution p-value = 7.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 192 total aligned reads displayed.

CCCCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAG  >  CP009273/3767265‑3767551
                                                                                                                                                 |                                                                                                                                             
     aCTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGATGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTa                                                                                                                                        >  2:2715854/1‑148 (MQ=255)
      cTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTAt                                                                                                                                       >  2:3324653/1‑148 (MQ=255)
      cTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTAt                                                                                                                                       >  2:1549995/1‑148 (MQ=255)
       ttATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTAtt                                                                                                                                      >  1:162135/1‑148 (MQ=255)
        tATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATtg                                                                                                                                     <  1:2564210/148‑1 (MQ=255)
        tATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATtg                                                                                                                                     <  1:1246112/148‑1 (MQ=255)
        tATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATtg                                                                                                                                     <  1:2055519/148‑1 (MQ=255)
          tCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATtgct                                                                                                                                   >  2:2185148/1‑148 (MQ=255)
          gcTGGGTGAGTCTATCGCGGTGCCACACGGGACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGGGTTCTGCCAGTACCCGGAAGGCGTGCGCTTGGGTGACGTAGAAGATGACATTGGCCGTCGGGTTCTTGGGATtgct                                                                                                                                   >  2:2184961/2‑148 (MQ=255)
           cTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATtgctg                                                                                                                                  >  2:232618/1‑148 (MQ=255)
                  aGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTaa                                                                                                                           >  1:1137598/1‑148 (MQ=255)
                    tCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTaaca                                                                                                                         <  2:1090308/148‑1 (MQ=255)
                     cTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTaacaa                                                                                                                        <  2:2767685/148‑1 (MQ=255)
                     cTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTaacaa                                                                                                                        >  1:2689708/1‑148 (MQ=255)
                     cTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTaacaa                                                                                                                        <  1:2230898/148‑1 (MQ=255)
                         cgcgGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAg                                                                                                                    >  1:593715/1‑148 (MQ=255)
                         cgcgGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAg                                                                                                                    >  2:2980649/1‑148 (MQ=255)
                           cgGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGca                                                                                                                  <  2:1291781/148‑1 (MQ=255)
                           cgGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGca                                                                                                                  >  1:532315/1‑148 (MQ=255)
                                ccACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTc                                                                                                             >  2:1741924/1‑148 (MQ=255)
                                   cacGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAgg                                                                                                          <  2:1978688/148‑1 (MQ=255)
                                   cacGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAgg                                                                                                          >  1:486006/1‑148 (MQ=255)
                                   cacGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAgg                                                                                                          >  1:3424538/1‑148 (MQ=255)
                                     cggtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGtt                                                                                                        <  1:1825440/148‑1 (MQ=255)
                                     cggtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGtt                                                                                                        >  2:2387892/1‑148 (MQ=255)
                                     cggtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGGGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGtt                                                                                                        >  2:3218334/1‑148 (MQ=255)
                                       gtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTAt                                                                                                      <  1:3269299/148‑1 (MQ=255)
                                          ctgtTGAAGCGAAAGATCGAGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGAAATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCAc                                                                                                   <  1:3423909/146‑1 (MQ=255)
                                          cggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCAc                                                                                                   <  1:768779/148‑1 (MQ=255)
                                           ggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCAcc                                                                                                  <  2:273076/148‑1 (MQ=255)
                                           ggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCAcc                                                                                                  <  1:233/148‑1 (MQ=255)
                                            gtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCa                                                                                                 >  2:3313308/1‑148 (MQ=255)
                                              tGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGc                                                                                               <  1:3783222/148‑1 (MQ=255)
                                              tGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGc                                                                                               <  2:2964613/148‑1 (MQ=255)
                                                 aGCGAATGATCGCGTACTGATAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTg                                                                                            <  2:631772/148‑1 (MQ=255)
                                                 aGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTg                                                                                            <  2:1724511/148‑1 (MQ=255)
                                                  gCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCCGCCTGa                                                                                           >  2:2713992/1‑148 (MQ=255)
                                                     tatgATCGCGTACTGAAAAGGGGCGTCGTGTTGTTCCAGTACCCGTAAGGCGTTCGGTTCGGTGAAGAAGAAGATGACTTTGCCCGTCTGGTTATTGGTATTGCTGCCTGTAAGAAGGAGGACATTCTGGTTATCACCAGCCTGACCa                                                                                        <  2:102635/145‑1 (MQ=255)
                                                     aaaGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCa                                                                                        <  2:102525/148‑1 (MQ=255)
                                                      aaGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCaa                                                                                       >  2:3348986/1‑148 (MQ=255)
                                                        gATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATg                                                                                     >  2:810541/1‑148 (MQ=255)
                                                          tCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCa                                                                                   <  2:2539028/148‑1 (MQ=255)
                                                          tCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCa                                                                                   >  1:1488566/1‑148 (MQ=255)
                                                          tCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCa                                                                                   >  1:2295129/1‑148 (MQ=255)
                                                            gcgTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACt                                                                                 <  2:2689708/148‑1 (MQ=255)
                                                              gTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACAGTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTgg                                                                               <  2:3255949/148‑1 (MQ=255)
                                                                 cTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGgatg                                                                            <  1:2954461/148‑1 (MQ=255)
                                                                  tGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGgatga                                                                           >  1:651097/1‑148 (MQ=255)
                                                                  tGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGgatga                                                                           <  2:445126/148‑1 (MQ=255)
                                                                       aCGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGt                                                                      >  1:3786327/1‑148 (MQ=255)
                                                                       aCGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGt                                                                      >  1:1714120/1‑148 (MQ=255)
                                                                       aCGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGt                                                                      <  2:3272847/148‑1 (MQ=255)
                                                                        cGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTc                                                                     >  2:3540366/1‑148 (MQ=255)
                                                                          ggCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCg                                                                   <  2:893363/148‑1 (MQ=255)
                                                                              tcgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCAt                                                               <  1:1669003/148‑1 (MQ=255)
                                                                                 tgtTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGa                                                            >  1:1351536/1‑148 (MQ=255)
                                                                                 tgtTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGa                                                            >  1:1011975/1‑148 (MQ=255)
                                                                                   ttCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGc                                                          >  1:2629687/1‑148 (MQ=255)
                                                                                    tCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCg                                                         >  2:470041/1‑148 (MQ=255)
                                                                                     cTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGt                                                        <  1:2136904/148‑1 (MQ=255)
                                                                                      tGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTc                                                       >  1:2498128/1‑148 (MQ=255)
                                                                                      tGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTc                                                       <  2:1127909/148‑1 (MQ=255)
                                                                                      tGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTc                                                       <  1:3500419/148‑1 (MQ=255)
                                                                                        ccAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTg                                                     >  1:2255500/1‑148 (MQ=255)
                                                                                          aGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGc                                                   <  1:1741924/148‑1 (MQ=255)
                                                                                            tACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGcac                                                 >  2:2180203/1‑148 (MQ=255)
                                                                                              cccGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGcacac                                               <  1:2421576/148‑1 (MQ=255)
                                                                                                   aaGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACAcaccac                                          >  1:1352864/1‑148 (MQ=255)
                                                                                                   aaGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACAcaccac                                          >  2:2287535/1‑148 (MQ=255)
                                                                                                    aGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACaccacc                                         <  1:1508956/148‑1 (MQ=255)
                                                                                                    aGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACaccacc                                         <  1:1501248/148‑1 (MQ=255)
                                                                                                      gCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAg                                       >  1:113487/1‑148 (MQ=255)
                                                                                                       cGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGc                                      >  2:570520/1‑148 (MQ=255)
                                                                                                         tGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGt                                    >  2:3442940/1‑148 (MQ=255)
                                                                                                          gcgcTTCGGTGAAGAAGAAGATGACATTGCCCGTCTTGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTg                                   >  1:2761356/1‑148 (MQ=255)
                                                                                                           cgcTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTgg                                  <  2:3499501/148‑1 (MQ=255)
                                                                                                            gcTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGa                                 <  1:2506319/148‑1 (MQ=255)
                                                                                                              ttCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATg                               >  1:127207/1‑148 (MQ=255)
                                                                                                                 ggTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAg                            >  1:3762624/1‑148 (MQ=255)
                                                                                                                       gaagaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTgg                      >  1:3266656/1‑148 (MQ=255)
                                                                                                                       gaagaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGACTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTgg                      >  1:1388350/1‑148 (MQ=255)
                                                                                                                        aagaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGa                     >  2:959418/1‑148 (MQ=255)
                                                                                                                        aagaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGa                     >  2:1283661/1‑148 (MQ=255)
                                                                                                                         agaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGaa                    <  2:140701/148‑1 (MQ=255)
                                                                                                                          gaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAAc                   >  2:2596820/1‑148 (MQ=255)
                                                                                                                          gaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAAc                   >  2:2596324/1‑148 (MQ=255)
                                                                                                                            agaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActg                 >  1:720531/1‑148 (MQ=255)
                                                                                                                            agaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActg                 <  1:3685577/148‑1 (MQ=255)
                                                                                                                            agaTGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActg                 <  2:2481881/148‑1 (MQ=255)
                                                                                                                            agaTGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActg                 <  2:162178/148‑1 (MQ=255)
                                                                                                                             gaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActgc                >  2:937286/1‑148 (MQ=255)
                                                                                                                                  cATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGGACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCa           >  2:1854940/1‑148 (MQ=255)
                                                                                                                                  cATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCa           >  2:3745519/1‑148 (MQ=255)
                                                                                                                                  cATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCa           >  1:2995967/1‑148 (MQ=255)
                                                                                                                                   aTTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAg          <  2:2892267/148‑1 (MQ=255)
                                                                                                                                    ttGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAgg         <  1:3156303/148‑1 (MQ=255)
                                                                                                                                     tGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGt        <  1:951849/148‑1 (MQ=255)
                                                                                                                                     tGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGt        >  1:1943139/1‑148 (MQ=255)
                                                                                                                                        ccGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCgt     >  1:2685359/1‑148 (MQ=255)
                                                                                                                                           tCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCgtaag  >  1:2404092/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
CCCCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAG  >  CP009273/3767265‑3767551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑