Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,774,728 C→A 5.3% H52N (CAC→AAC)  trmL → tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,774,7280CA 5.3% 1053.7 / 17.5 272H52N (CAC→AAC) trmLtRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base C (129/128);  new base A (6/9);  total (135/137)
Fisher's exact test for biased strand distribution p-value = 5.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 283 total aligned reads displayed.

AACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATCAGGATGGCGATTATCTGATGTTCGGCC  >  CP009273/3774581‑3774875
                                                                                                                                                   |                                                                                                                                                   
aaCATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATc                                                                                                                                                     >  2:139028/1‑148 (MQ=255)
aaCATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATc                                                                                                                                                     >  1:257529/1‑148 (MQ=255)
aaCATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATc                                                                                                                                                     >  1:2527135/1‑148 (MQ=255)
aaCATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGACTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATc                                                                                                                                                     <  2:2266801/148‑1 (MQ=255)
    tCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGa                                                                                                                                                 >  2:2955131/1‑148 (MQ=255)
    tCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGa                                                                                                                                                 >  2:3499143/1‑148 (MQ=255)
     cGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAg                                                                                                                                                <  1:1008576/148‑1 (MQ=255)
          tttACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTAc                                                                                                                                           >  1:1718726/1‑148 (MQ=255)
                  ccAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATAACGAGTTTACCGCCGTTa                                                                                                                                   >  2:2642462/1‑148 (MQ=255)
                   cAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTAc                                                                                                                                  >  2:1740891/1‑148 (MQ=255)
                     gAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTAcgc                                                                                                                                >  1:335900/1‑148 (MQ=255)
                     gAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTAcgc                                                                                                                                >  1:1363419/1‑148 (MQ=255)
                       aaTTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGt                                                                                                                              <  1:586428/148‑1 (MQ=255)
                        aTTCCGCCAAATACGGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGtc                                                                                                                             <  1:948466/148‑1 (MQ=255)
                         ttCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGtca                                                                                                                            >  1:3803614/1‑148 (MQ=255)
                         ttCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGtca                                                                                                                            >  2:749132/1‑148 (MQ=255)
                         ttCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTGACCGCCGTTACGCGtca                                                                                                                            <  1:1208461/148‑1 (MQ=255)
                          tCCGCCAAATCCGGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGtcat                                                                                                                           <  1:2014671/148‑1 (MQ=255)
                            cGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGtcatca                                                                                                                         <  2:3734069/148‑1 (MQ=255)
                              ccAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATg                                                                                                                       <  2:3368990/148‑1 (MQ=255)
                              ccAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATg                                                                                                                       <  2:1205318/148‑1 (MQ=255)
                                aaaTACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGAc                                                                                                                     >  2:1058279/1‑148 (MQ=255)
                                 caTACTGGCAACATAATCCGTGGTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCTATGGTATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACt                                                                                                                    <  1:3231479/147‑1 (MQ=255)
                                  aTACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTa                                                                                                                   >  2:3636294/1‑148 (MQ=255)
                                   tACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTAt                                                                                                                  <  2:745403/148‑1 (MQ=255)
                                       ggCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATcgcg                                                                                                              >  2:3764780/1‑148 (MQ=255)
                                        gCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATcgcgc                                                                                                             >  2:3103573/1‑148 (MQ=255)
                                           aCATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGtt                                                                                                          <  2:1799305/148‑1 (MQ=255)
                                            catcatCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTc                                                                                                         <  1:868943/148‑1 (MQ=255)
                                            catcatCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTc                                                                                                         <  1:3415652/148‑1 (MQ=255)
                                             atcatcCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTcc                                                                                                        <  2:2176262/148‑1 (MQ=255)
                                             atcatcCGTCTTTGCGCTAAGACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTcc                                                                                                        <  2:1203427/148‑1 (MQ=255)
                                              tcatcCGTCTTTGCGCTACTACCGGCGTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCt                                                                                                       <  2:11228/148‑1 (MQ=255)
                                              tcatcCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGACCCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCt                                                                                                       <  2:3347248/148‑1 (MQ=255)
                                              tcatcCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCt                                                                                                       <  2:3659779/148‑1 (MQ=255)
                                              tcatcCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCt                                                                                                       >  1:1591560/1‑148 (MQ=255)
                                               catcCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTc                                                                                                      >  1:3568828/1‑148 (MQ=255)
                                                atcCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCg                                                                                                     >  2:1675256/1‑148 (MQ=255)
                                                 tcCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGa                                                                                                    >  2:2859246/1‑148 (MQ=255)
                                                 tcCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGa                                                                                                    >  1:1180290/1‑148 (MQ=255)
                                                    gTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGc                                                                                                 >  2:3788240/1‑148 (MQ=255)
                                                     tCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCa                                                                                                >  2:2605046/1‑148 (MQ=255)
                                                      cTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAg                                                                                               >  1:1266147/1‑148 (MQ=255)
                                                             cTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATcc                                                                                        <  1:2072505/148‑1 (MQ=255)
                                                               aaTACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATcccc                                                                                      <  2:2473360/148‑1 (MQ=255)
                                                                 tACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAg                                                                                    >  2:425994/1‑148 (MQ=255)
                                                                  aCCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAgc                                                                                   >  1:3434717/1‑148 (MQ=255)
                                                                  aCCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAgc                                                                                   >  2:154773/1‑148 (MQ=255)
                                                                    cGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAgcgc                                                                                 <  1:471315/148‑1 (MQ=255)
                                                                    cGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAgcgc                                                                                 <  1:1896477/148‑1 (MQ=255)
                                                                      gCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCt                                                                               >  2:193736/1‑148 (MQ=255)
                                                                        tttCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGt                                                                             <  1:2510672/148‑1 (MQ=255)
                                                                              cTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGccc                                                                       >  2:650513/1‑148 (MQ=255)
                                                                                 cATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTca                                                                    <  2:1225133/148‑1 (MQ=255)
                                                                                  atatCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTcac                                                                   <  2:1912265/148‑1 (MQ=255)
                                                                                   tatCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTcacc                                                                  >  2:958614/1‑148 (MQ=255)
                                                                                      catcGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACg                                                               >  1:3718595/1‑148 (MQ=255)
                                                                                        tcGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGaa                                                             >  1:657796/1‑148 (MQ=255)
                                                                                         cGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGaaa                                                            <  2:2767281/148‑1 (MQ=255)
                                                                                         cGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGaaa                                                            >  1:2140105/1‑148 (MQ=255)
                                                                                         cGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGaaa                                                            >  1:3386187/1‑148 (MQ=255)
                                                                                          taaCCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAg                                                           <  2:2328775/147‑1 (MQ=255)
                                                                                           aaCCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAgg                                                          <  1:2211965/148‑1 (MQ=255)
                                                                                            aCCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATAACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGt                                                         >  1:2283550/1‑148 (MQ=255)
                                                                                             ccGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTa                                                        <  1:3297460/148‑1 (MQ=255)
                                                                                                aTGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTacac                                                     <  2:739520/148‑1 (MQ=255)
                                                                                                 tGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACAcc                                                    >  2:3062534/1‑148 (MQ=255)
                                                                                                   ggATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTg                                                  >  1:2725532/1‑148 (MQ=255)
                                                                                                   cgATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTg                                                  <  2:3519005/147‑1 (MQ=255)
                                                                                                    gATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGc                                                 >  2:1268168/1‑148 (MQ=255)
                                                                                                         gCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTcaca                                            <  1:3521447/148‑1 (MQ=255)
                                                                                                            tGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAgcg                                         >  1:3626386/1‑148 (MQ=255)
                                                                                                                aCGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGt                                     <  1:3125306/148‑1 (MQ=255)
                                                                                                                 cGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTa                                    <  2:2527004/148‑1 (MQ=255)
                                                                                                                 cGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTa                                    <  1:1065941/148‑1 (MQ=255)
                                                                                                                   aTAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAg                                  <  1:2826969/148‑1 (MQ=255)
                                                                                                                    tAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGc                                 >  2:3708412/1‑148 (MQ=255)
                                                                                                                     aaGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCt                                <  1:3788219/148‑1 (MQ=255)
                                                                                                                       gcgcCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTAt                              >  1:3064675/1‑148 (MQ=255)
                                                                                                                       gcgcCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTAt                              >  1:293390/1‑148 (MQ=255)
                                                                                                                        cgcCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATc                             >  2:932979/1‑148 (MQ=255)
                                                                                                                         gcCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATCa                            <  2:2641481/148‑1 (MQ=255)
                                                                                                                            tGCGCCGCGCGGGGCTGGACTATAACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATCAGGa                         <  2:3163894/148‑1 (MQ=255)
                                                                                                                             gcgcCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATCAGGAt                        >  1:1572606/1‑148 (MQ=255)
                                                                                                                             gcgcCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATCAGGAt                        <  1:674513/148‑1 (MQ=255)
                                                                                                                             gcgcCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATCAGGAt                        <  1:1601478/148‑1 (MQ=255)
                                                                                                                                 cgcgcgGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATCAGGATGGCg                    <  1:1940880/148‑1 (MQ=255)
                                                                                                                                  gcgcgGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATCAGGATGGCGa                   <  2:2605257/148‑1 (MQ=255)
                                                                                                                                    gcgGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATCAGGATGGCGAtt                 <  2:1190392/148‑1 (MQ=255)
                                                                                                                                     cgGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATCAGGATGGCGATTa                <  1:1808000/148‑1 (MQ=255)
                                                                                                                                     cgGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATCAGGATGGCGATTa                <  1:2605049/148‑1 (MQ=255)
                                                                                                                                      ggggCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATCAGGATGGCGATTAt               <  2:1576465/148‑1 (MQ=255)
                                                                                                                                         gCTGGACTATCACGAGATTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATCAGGATGGCGATTATCTg            <  1:1327281/148‑1 (MQ=255)
                                                                                                                                            ggACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATCAGGATGGCGATTATCTGATg         <  2:3267276/148‑1 (MQ=255)
                                                                                                                                            ggACTATAACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATCAGGATGGCGATTATCTGATg         <  2:3600094/148‑1 (MQ=255)
                                                                                                                                             gACTATAACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATCAGGATGGCGATTATCTGATGt        >  1:3469334/1‑148 (MQ=255)
                                                                                                                                             gACTATAACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATCAGGATGGCGATTATCTGATGt        <  1:2642377/148‑1 (MQ=255)
                                                                                                                                             gACTATAACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATCAGGATGGCGATTATCTGATGt        <  1:2642462/148‑1 (MQ=255)
                                                                                                                                              aCTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATCAGGATGGCGATTATCTGATGtt       <  2:677869/148‑1 (MQ=255)
                                                                                                                                                 aTCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATCAGGATGGCGATTATCTGATGTTCgg    >  2:826483/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTTTCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGTTTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTCCTCGAAGCAGAAAATCCCCAGCGCCTGTTCGCCCTCACCACGAAAGGTACACCTGCTCACAGCGCCGTAAGCTATCAGGATGGCGATTATCTGATGTTCGGCC  >  CP009273/3774581‑3774875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑