Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,789,381 C→A 100% L15I (CTC→ATC)  waaC → ADP‑heptose:LPS heptosyl transferase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,789,3810CA97.9% 612.8 / 3.7 195L15I (CTC→ATC) waaCADP‑heptose:LPS heptosyl transferase I
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (101/90);  total (103/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 201 total aligned reads displayed.

GGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGC  >  CP009273/3789237‑3789525
                                                                                                                                                |                                                                                                                                                
ggTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATcc                                                                                                                                               >  2:1438185/1‑148 (MQ=255)
ggTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATcc                                                                                                                                               <  1:995050/148‑1 (MQ=255)
ggTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATcc                                                                                                                                               >  1:893350/1‑148 (MQ=255)
ggTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATcc                                                                                                                                               >  1:2044601/1‑148 (MQ=255)
  tGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCAt                                                                                                                                             >  1:3186257/1‑148 (MQ=255)
   gACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATa                                                                                                                                            <  2:146603/148‑1 (MQ=255)
   gACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATa                                                                                                                                            <  2:882297/148‑1 (MQ=255)
     ggCTGGGGAGGTTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCTACGCGATATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACg                                                                                                                                          <  2:3747723/147‑1 (MQ=255)
       cTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGtt                                                                                                                                        >  2:3563167/1‑148 (MQ=255)
        tGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTg                                                                                                                                       <  2:3624678/148‑1 (MQ=255)
               ggTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGcac                                                                                                                                >  1:3050327/1‑148 (MQ=255)
                 ttATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGcactc                                                                                                                              >  1:3679410/1‑148 (MQ=255)
                  tATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGcactca                                                                                                                             <  2:1577505/148‑1 (MQ=255)
                  tATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGcactca                                                                                                                             >  2:126156/1‑148 (MQ=255)
                    tCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGcactcact                                                                                                                           >  1:1433439/1‑148 (MQ=255)
                      aCCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGa                                                                                                                         <  2:970234/148‑1 (MQ=255)
                       ccAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGAt                                                                                                                        >  1:1814417/1‑148 (MQ=255)
                          gagCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGcc                                                                                                                     <  1:3731605/148‑1 (MQ=255)
                          gagCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGcc                                                                                                                     >  1:3799084/1‑148 (MQ=255)
                           agCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGccc                                                                                                                    >  2:2724874/1‑148 (MQ=255)
                           accTTAATCGACAAAACTCCCCAGCGCGAACTCGAAGAACTCAACCCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGAAATCACTGATcccc                                                                                                                    >  2:3480943/3‑148 (MQ=255)
                            gCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCca                                                                                                                   >  1:1493120/1‑148 (MQ=255)
                             cTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCcag                                                                                                                  >  2:714639/1‑148 (MQ=255)
                              ttAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCcagc                                                                                                                 <  1:1199108/148‑1 (MQ=255)
                                   cGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCa                                                                                                            >  1:1659427/1‑148 (MQ=255)
                                   cGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCa                                                                                                            <  2:2154243/148‑1 (MQ=255)
                                      cATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTtatc                                                                                                                                                <  2:3810180/109‑1 (MQ=255)
                                      cATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTtatc                                                                                                                                                >  1:3810289/1‑109 (MQ=255)
                                      cATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTtatc                                                                                                                                                >  1:3808767/1‑109 (MQ=255)
                                      cATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATc                                                                                                         <  1:1013922/148‑1 (MQ=255)
                                       aTTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATcc                                                                                                        <  2:3229406/148‑1 (MQ=255)
                                         tACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCa                                                                                                      >  2:616800/1‑148 (MQ=255)
                                         tACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCa                                                                                                      >  1:826508/1‑148 (MQ=255)
                                          aCTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAg                                                                                                     <  2:1804109/148‑1 (MQ=255)
                                          aCTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAg                                                                                                     >  2:2427744/1‑148 (MQ=255)
                                          aCTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAg                                                                                                     >  2:2427900/1‑148 (MQ=255)
                                             ccccAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGa                                                                                                  >  1:3532274/1‑148 (MQ=255)
                                             ccccAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGa                                                                                                  <  1:1826623/148‑1 (MQ=255)
                                                 aGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTaa                                                                                              <  1:3649152/148‑1 (MQ=255)
                                                     cgTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGttt                                                                                          <  2:2577519/148‑1 (MQ=255)
                                                      gTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTg                                                                                         <  1:3575138/148‑1 (MQ=255)
                                                      gTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTg                                                                                         <  1:2178205/148‑1 (MQ=255)
                                                       tACTGGAAGAACTCAACGCGCTATTGTTACAATAGGAAGCCTGACGTATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATACATACGTTGACCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGa                                                                                        >  1:2853039/1‑148 (MQ=255)
                                                        aCTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGAc                                                                                       >  2:630226/1‑148 (MQ=255)
                                                               gaaCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGggtgg                                                                                >  2:258393/1‑148 (MQ=255)
                                                                aaCTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGggtggt                                                                               <  2:2606324/148‑1 (MQ=255)
                                                                   tCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGga                                                                            <  2:487827/148‑1 (MQ=255)
                                                                       cgcgCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGgacgaa                                                                        >  2:3354541/1‑148 (MQ=255)
                                                                       cgcgCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGgaagaa                                                                        >  2:379776/1‑148 (MQ=255)
                                                                        gcgcTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGaagaag                                                                       <  1:979224/148‑1 (MQ=255)
                                                                        gcgcTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGaagaag                                                                       <  1:1914840/148‑1 (MQ=255)
                                                                         cgcTATTGTTACCAGAGGAAGCCTGTCGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAgg                                                                      <  1:1119123/148‑1 (MQ=255)
                                                                         cgcTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAgg                                                                      <  1:232616/148‑1 (MQ=255)
                                                                           cTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGt                                                                    <  2:1245627/148‑1 (MQ=255)
                                                                            tATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCACTCCCCGGGATGAAGTTTGACTGGGTGGTGGAAGAAGGGtt                                                                   >  2:383124/1‑148 (MQ=255)
                                                                            tATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGtt                                                                   >  2:3028028/1‑148 (MQ=255)
                                                                            tATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGtt                                                                   >  2:382772/1‑148 (MQ=255)
                                                                            tATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGtt                                                                   >  2:383115/1‑148 (MQ=255)
                                                                            tATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGGCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGtt                                                                   <  1:3464268/148‑1 (MQ=255)
                                                                               tGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGc                                                                >  1:1258001/1‑148 (MQ=255)
                                                                               tGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGc                                                                >  1:1380064/1‑148 (MQ=255)
                                                                               tGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGc                                                                >  1:1728013/1‑148 (MQ=255)
                                                                                 ttACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGcac                                                              <  1:311443/148‑1 (MQ=255)
                                                                                 ttACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGcac                                                              >  1:3074632/1‑148 (MQ=255)
                                                                                    cAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGa                                                           <  1:1943337/148‑1 (MQ=255)
                                                                                     aaGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAt                                                          <  2:1570543/148‑1 (MQ=255)
                                                                                     aaGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAt                                                          <  1:68151/148‑1 (MQ=255)
                                                                                     aaGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAt                                                          <  1:2963793/148‑1 (MQ=255)
                                                                                        agGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAttcc                                                       <  2:2916563/148‑1 (MQ=255)
                                                                                           aaGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAttccttc                                                    >  2:1365667/1‑148 (MQ=255)
                                                                                             gCCTGACGGAAGCGGGGTTTGTTCGTTAAAACATCGTCGAAGGGCTATGTTATCCATACGTTGCCCCCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTCAGTATGACTGGGTGGTGGAAGAAGGGTGCGCACAGATtccttcct                                                  >  2:2219585/1‑148 (MQ=255)
                                                                                                    ggATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGc                                           >  1:1571011/1‑148 (MQ=255)
                                                                                                       tGCGGGTTTTGATCGTTAAAACATCGTCGCTGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGc                                        <  2:3210930/148‑1 (MQ=255)
                                                                                                        gCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGcc                                       >  2:1127389/1‑148 (MQ=255)
                                                                                                           ggTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGtt                                    >  2:1102170/1‑148 (MQ=255)
                                                                                                             ttttGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGa                                  >  2:3817676/1‑148 (MQ=255)
                                                                                                             ttttGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGa                                  >  2:912197/1‑148 (MQ=255)
                                                                                                              tttGATCGTTAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAg                                 >  1:1557875/1‑148 (MQ=255)
                                                                                                                      ttAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTAt                         >  2:3793212/1‑148 (MQ=255)
                                                                                                                       tAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTAtt                        >  2:3301069/1‑148 (MQ=255)
                                                                                                                       tAAAACATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTAtt                        >  1:1511633/1‑148 (MQ=255)
                                                                                                                           aCATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCtg                    >  1:1380153/1‑148 (MQ=255)
                                                                                                                           aCATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCtg                    <  1:1575069/148‑1 (MQ=255)
                                                                                                                           aCATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCtg                    <  1:2522838/148‑1 (MQ=255)
                                                                                                                            cATCGTCGATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCtgt                   >  2:1429269/1‑148 (MQ=255)
                                                                                                                              tcgtcgATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTgg                 >  2:464888/1‑148 (MQ=255)
                                                                                                                                gtcgATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCa               <  2:1308926/148‑1 (MQ=255)
                                                                                                                                  cgATGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAAt             <  2:2779584/148‑1 (MQ=255)
                                                                                                                                    aTGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATAc           >  2:3520247/1‑148 (MQ=255)
                                                                                                                                    aTGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATAc           <  2:3516481/148‑1 (MQ=255)
                                                                                                                                    aTGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATAc           >  2:3179738/1‑148 (MQ=255)
                                                                                                                                    aTGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATAc           <  2:508847/148‑1 (MQ=255)
                                                                                                                                    aTGGGCGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATAc           >  1:1051257/1‑148 (MQ=255)
                                                                                                                                      gggCGATGTTATCCATACGGTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGt         <  1:450452/148‑1 (MQ=255)
                                                                                                                                         cGATGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGc      <  2:2871749/148‑1 (MQ=255)
                                                                                                                                           aTGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGATg    >  2:2292633/1‑148 (MQ=255)
                                                                                                                                            tGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTgg   >  2:457625/1‑148 (MQ=255)
                                                                                                                                            tGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTgg   >  1:732462/1‑148 (MQ=255)
                                                                                                                                            tGTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTgg   >  1:1515975/1‑148 (MQ=255)
                                                                                                                                             gTTATCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGc  >  2:2696100/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTACAAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCTGTGGCAATACGTCGCTGGC  >  CP009273/3789237‑3789525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑