Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,814,866 A→C 6.8% T27P (ACC→CCC)  gmk → guanylate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,814,8660AC 6.8% 656.4 / 38.7 236T27P (ACC→CCC) gmkguanylate kinase
Reads supporting (aligned to +/- strand):  ref base A (105/115);  new base C (12/4);  total (117/119)
Fisher's exact test for biased strand distribution p-value = 4.04e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 240 total aligned reads displayed.

CATGGCCGCGCCGTGTATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGCGTTCCTCGAACACGC  >  CP009273/3814719‑3815011
                                                                                                                                                   |                                                                                                                                                 
   ggCCGCGCCGTGTATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAAccc                                                                                                                                                >  2:1272978/1‑148 (MQ=255)
     ccGCGCCGTGTATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCaa                                                                                                                                              <  1:2119976/148‑1 (MQ=255)
        cgcCGTGTATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCg                                                                                                                                           <  2:1586397/148‑1 (MQ=255)
        cgcCGTGTATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCg                                                                                                                                           <  1:3493240/148‑1 (MQ=255)
             tgtATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTa                                                                                                                                      >  2:3044670/1‑148 (MQ=255)
             tgtATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTa                                                                                                                                      >  1:664229/1‑148 (MQ=255)
             tgtATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATACAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTa                                                                                                                                      >  2:1534752/1‑148 (MQ=255)
             tgtATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGAAACTCATGGCTCACGGCACGCTTTATATTGTATCTGCCCCCAGAGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTa                                                                                                                                      >  2:1874853/1‑148 (MQ=255)
              gtATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTAt                                                                                                                                     <  1:2629078/148‑1 (MQ=255)
                     aaGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACAccc                                                                                                                              <  2:1214422/148‑1 (MQ=255)
                     aaGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACAccc                                                                                                                              <  1:2155520/148‑1 (MQ=255)
                           gTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGttt                                                                                                                        <  2:697683/148‑1 (MQ=255)
                           gTATGTAGGATTTTTTTCGCTAATCACATACGAAAGATTCTCATGGCTGAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTTAATCCTGCCTGATTCAGGCTTTATTAAAAACCCAACGGTTGTATGACACCCAGGttt                                                                                                                        <  1:2432854/148‑1 (MQ=255)
                            tATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTc                                                                                                                       >  2:2059687/1‑148 (MQ=255)
                             aTGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCt                                                                                                                      >  2:55214/1‑148 (MQ=255)
                              tGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTg                                                                                                                     >  1:1061529/1‑148 (MQ=255)
                              tGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTg                                                                                                                     <  1:1796295/148‑1 (MQ=255)
                                 aGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGttt                                                                                                                  <  1:2054133/148‑1 (MQ=255)
                                  ggCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAACCCCAACCGTTGTATGACACCCAGGTTTCTGTTTc                                                                                                                 >  1:3189848/1‑148 (MQ=255)
                                  ggCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTc                                                                                                                 >  1:3128428/1‑148 (MQ=255)
                                     tttATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAACCCCAACCGTTGTATGACACCCAGGTTTCTGTTTcaca                                                                                                              <  1:3096998/148‑1 (MQ=255)
                                            cGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCAcacaccaca                                                                                                       <  1:1500890/148‑1 (MQ=255)
                                              cTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACg                                                                                                     <  1:2414577/148‑1 (MQ=255)
                                                 aTCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCa                                                                                                  <  2:1042978/148‑1 (MQ=255)
                                                 aTCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCa                                                                                                  >  1:1620273/1‑148 (MQ=255)
                                                   cacaTACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAAc                                                                                                >  1:1775306/1‑148 (MQ=255)
                                                   cacaTACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAAc                                                                                                <  2:3067645/148‑1 (MQ=255)
                                                     caTACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACcg                                                                                              >  1:932307/1‑148 (MQ=255)
                                                     caTACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACcg                                                                                              <  1:3120003/148‑1 (MQ=255)
                                                        aCGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGt                                                                                           >  2:3489704/1‑148 (MQ=255)
                                                        aCGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGt                                                                                           >  2:1592717/1‑148 (MQ=255)
                                                        aCGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGt                                                                                           >  2:3792541/1‑148 (MQ=255)
                                                         cGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTc                                                                                          <  1:469350/148‑1 (MQ=255)
                                                         cGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTc                                                                                          <  1:765556/148‑1 (MQ=255)
                                                           aaaGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAACCCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCt                                                                                        >  1:3216155/1‑148 (MQ=255)
                                                           aaaGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCt                                                                                        <  1:129629/148‑1 (MQ=255)
                                                             aGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTgg                                                                                      <  1:3200639/148‑1 (MQ=255)
                                                               aTACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTT‑‑CACACCACACGCCAACCGCGTCCTGGTGaa                                                                                  <  2:3259401/148‑1 (MQ=255)
                                                                 aCTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGaa                                                                                  >  2:2735470/1‑148 (MQ=255)
                                                                   tCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGt                                                                                >  1:3397670/1‑148 (MQ=255)
                                                                    cATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTc                                                                               <  2:1804386/148‑1 (MQ=255)
                                                                    cATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTCCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTc                                                                               <  2:2123592/148‑1 (MQ=255)
                                                                      tGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCa                                                                             <  2:749268/148‑1 (MQ=255)
                                                                       ggCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCAc                                                                            >  2:3115163/1‑148 (MQ=255)
                                                                        gCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACg                                                                           <  1:1169149/148‑1 (MQ=255)
                                                                         cTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACgg                                                                          >  1:3316615/1‑148 (MQ=255)
                                                                         cTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACgg                                                                          >  2:687747/1‑148 (MQ=255)
                                                                          tCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGt                                                                         <  1:2832396/148‑1 (MQ=255)
                                                                          tCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGt                                                                         <  1:2543449/148‑1 (MQ=255)
                                                                             aGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGaa                                                                      >  1:2768914/1‑148 (MQ=255)
                                                                              ggCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAAc                                                                     >  2:2481953/1‑148 (MQ=255)
                                                                               gCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACa                                                                    <  1:1448437/148‑1 (MQ=255)
                                                                               gCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACa                                                                    <  2:2985435/148‑1 (MQ=255)
                                                                                cACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACat                                                                   >  1:2019504/1‑148 (MQ=255)
                                                                                 aCGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACatt                                                                  <  1:559892/148‑1 (MQ=255)
                                                                                  cGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACatta                                                                 <  1:1591348/148‑1 (MQ=255)
                                                                                   gCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACattat                                                                >  2:2020265/1‑148 (MQ=255)
                                                                                     tttATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTAttt                                                              >  1:1582170/1‑148 (MQ=255)
                                                                                         tatTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCttt                                                          >  2:780926/1‑148 (MQ=255)
                                                                                           ttGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGt                                                        <  1:1272978/148‑1 (MQ=255)
                                                                                           ttGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGt                                                        <  1:3812521/148‑1 (MQ=255)
                                                                                            tGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGtt                                                       >  2:1620321/1‑148 (MQ=255)
                                                                                             gTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTa                                                      <  1:169958/148‑1 (MQ=255)
                                                                                             gTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTa                                                      <  2:462215/148‑1 (MQ=255)
                                                                                                tCTGCCCCCAGTGGCGCGTGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATc                                                   <  1:985134/148‑1 (MQ=255)
                                                                                                tCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATc                                                   <  1:985181/148‑1 (MQ=255)
                                                                                                tCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACAGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATc                                                   <  2:683171/148‑1 (MQ=255)
                                                                                                tCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACAGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATc                                                   <  2:2600824/148‑1 (MQ=255)
                                                                                                 cTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCa                                                  <  1:33705/148‑1 (MQ=255)
                                                                                                 cTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCa                                                  <  2:3706659/148‑1 (MQ=255)
                                                                                                 cTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCa                                                  <  2:1178124/148‑1 (MQ=255)
                                                                                                   gCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCatg                                                >  1:2705946/1‑148 (MQ=255)
                                                                                                   gCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCatg                                                <  2:2561318/148‑1 (MQ=255)
                                                                                                    cccccAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCatga                                               <  1:490837/148‑1 (MQ=255)
                                                                                                    cccccAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCatga                                               <  2:2227795/148‑1 (MQ=255)
                                                                                                      cccAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCatgatg                                             <  2:2914827/148‑1 (MQ=255)
                                                                                                      cccAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCatgatg                                             >  1:57130/1‑148 (MQ=255)
                                                                                                        cAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGaa                                           <  1:3044670/148‑1 (MQ=255)
                                                                                                        cAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGaa                                           <  2:2037567/148‑1 (MQ=255)
                                                                                                         aGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAAt                                          <  1:2059687/148‑1 (MQ=255)
                                                                                                           tGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAAttt                                        >  1:1033711/1‑148 (MQ=255)
                                                                                                            ggCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTa                                       <  1:255462/148‑1 (MQ=255)
                                                                                                             gcgcGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTaa                                      <  1:2110430/148‑1 (MQ=255)
                                                                                                                 gggTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGaa                                  <  2:2846351/148‑1 (MQ=255)
                                                                                                                  ggTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGaaa                                 >  2:515686/1‑148 (MQ=255)
                                                                                                                  ggTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGaaa                                 <  1:1129262/148‑1 (MQ=255)
                                                                                                                   gTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAAt                                <  2:1503534/148‑1 (MQ=255)
                                                                                                                        tCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTa                           >  2:1118594/1‑148 (MQ=255)
                                                                                                                        tCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTa                           <  1:841372/148‑1 (MQ=255)
                                                                                                                         ccAGCCTGATTCAGGCTTTATTAAAACCCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAg                          >  1:980352/1‑148 (MQ=255)
                                                                                                                            gCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCag                       >  2:1273501/1‑148 (MQ=255)
                                                                                                                                 aTTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATg                  >  2:362974/1‑148 (MQ=255)
                                                                                                                                 aTTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATg                  >  2:1825578/1‑148 (MQ=255)
                                                                                                                                   tCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGCg                >  1:3436800/1‑148 (MQ=255)
                                                                                                                                    cAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGCGt               <  2:2355347/148‑1 (MQ=255)
                                                                                                                                      ggCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGCGTTc             >  1:49913/1‑148 (MQ=255)
                                                                                                                                      ggCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGCGTTc             <  1:2258705/148‑1 (MQ=255)
                                                                                                                                      ggCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGCGTTc             >  1:2235092/1‑148 (MQ=255)
                                                                                                                                       gCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGCGTTcc            >  1:446599/1‑148 (MQ=255)
                                                                                                                                             ttaAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGCGTTCCTCGAac      <  1:1210016/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CATGGCCGCGCCGTGTATAATAAGCTCGTATGTAGGCTTTATTTCGCTAATCACATACGAAAGATACTCATGGCTCAAGGCACGCTTTATATTGTTTCTGCCCCCAGTGGCGCGGGTAAATCCAGCCTGATTCAGGCTTTATTAAAAACCCAACCGTTGTATGACACCCAGGTTTCTGTTTCACACACCACACGCCAACCGCGTCCTGGTGAAGTCCACGGTGAACATTATTTCTTTGTTAATCATGATGAATTTAAAGAAATGATTAGCAGAGATGCGTTCCTCGAACACGC  >  CP009273/3814719‑3815011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑