Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,822,339 A→C 8.9% Q12P (CAA→CCA) ‡ xanP → xanthine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,822,3390AC 8.9% 408.8 / 3.5 206Q12P (CAA→CCA) ‡xanPxanthine permease
Reads supporting (aligned to +/- strand):  ref base A (91/90);  new base C (23/2);  total (114/92)
Fisher's exact test for biased strand distribution p-value = 5.50e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 208 total aligned reads displayed.

TATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGC  >  CP009273/3822192‑3822485
                                                                                                                                                   |                                                                                                                                                   
tataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCa                                                                                                                                                     >  2:2242367/1‑148 (MQ=255)
 ataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCaa                                                                                                                                                    <  1:1085529/148‑1 (MQ=255)
  taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAc                                                                                                                                                   >  1:750841/1‑148 (MQ=255)
  taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAc                                                                                                                                                   >  2:3497277/1‑148 (MQ=255)
   aaaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAcc                                                                                                                                                  >  1:2673285/1‑148 (MQ=255)
   aaaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAcc                                                                                                                                                  >  2:1557673/1‑148 (MQ=255)
    aaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCg                                                                                                                                                 <  2:2218983/148‑1 (MQ=255)
     aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAcccg                                                                                                                                                >  2:3754683/1‑146 (MQ=255)
     aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCgg                                                                                                                                                >  1:3005906/1‑148 (MQ=255)
      aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGCTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCAccccgg                                                                                                                                               >  1:1314519/1‑145 (MQ=255)
      aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGt                                                                                                                                               >  2:346812/1‑148 (MQ=255)
       aaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCGGtt                                                                                                                                              >  1:2824203/1‑148 (MQ=255)
       aaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGtt                                                                                                                                              >  1:724813/1‑148 (MQ=255)
       aaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTATCTTTCGGTCAGCTAAAATGCCCGCTTTTCTAACACTGGATTGTTTTCTATGTCTGTTTACACCCTCGAGTCAGAAAATGCGCAACAGGtt                                                                                                                                              >  2:2553560/1‑148 (MQ=255)
        aGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTg                                                                                                                                             >  2:1691007/1‑148 (MQ=255)
          tGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCAcccggtggg                                                                                                                                           >  1:341813/1‑141 (MQ=255)
            cATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCa                                                                                                                                        >  2:2100687/1‑148 (MQ=255)
            cATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTG‑CGCa                                                                                                                                        >  1:3427271/1‑148 (MQ=255)
             aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCCCCCTCGAGTCAGAAAATGCGCAACCGGTGT‑CGCAg                                                                                                                                       >  2:2142453/1‑148 (MQ=255)
               ttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGAc                                                                                                                                     >  2:3621293/1‑148 (MQ=255)
                  tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCCGACTCa                                                                                                                                  >  2:1138161/1‑148 (MQ=255)
                  tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCa                                                                                                                                  >  2:2939606/1‑148 (MQ=255)
                      aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACTGGTTG‑CGCAGACTCAAAAc                                                                                                                              <  1:2750116/148‑1 (MQ=255)
                       tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACa                                                                                                                             >  1:1105912/1‑148 (MQ=255)
                        ttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTG‑CGCAGACACaaaaaat                                                                                                                            >  1:1259510/1‑145 (MQ=255)
                          aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCGGGTTG‑CGCAGACTCAAAACAGCg                                                                                                                          <  1:3143226/148‑1 (MQ=255)
                          aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAACCGTTG‑CGCAGACTCAAAACatcc                                                                                                                          >  1:2441273/1‑145 (MQ=255)
                           aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGAGTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTG‑CGCAGACTCAAAACAGCGa                                                                                                                         >  2:3605961/1‑148 (MQ=255)
                            atatTTAGATGAGTATGTGGCAAACTTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTTTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGaa                                                                                                                        <  2:3105638/148‑1 (MQ=255)
                            atatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGaa                                                                                                                        >  2:2759776/1‑148 (MQ=255)
                              atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAAca                                                                                                                      >  2:1872803/1‑147 (MQ=255)
                               tttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTG‑CGCAGACTCAAAAAAACGAAATg                                                                                                                     >  1:1608597/1‑148 (MQ=255)
                               tttAGATGAGGATGTGGCAAACGTTGGCTTTTCTCTTTCGGTCAGCAAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGAGGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTg                                                                                                                     <  2:2671287/148‑1 (MQ=255)
                                ttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCACAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGa                                                                                                                    <  2:1187011/148‑1 (MQ=255)
                                  aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGAtt                                                                                                                  >  2:483238/1‑148 (MQ=255)
                                  aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGAtt                                                                                                                  >  2:401841/1‑148 (MQ=255)
                                    ttgtgtttgtgCCAAAGGTTTGGTTTTCTGTTTCGGTCAGATAAAATGCCCGCTTTGCTACCAGGGTTTTGTTTTCGTTGTGTGTGTCCACCCACGAGGCATAAAATGCGCACCCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTa                                                                                                                <  1:2203219/141‑1 (MQ=255)
                                    aTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAATTGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTa                                                                                                                <  2:2874652/148‑1 (MQ=255)
                                    aTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTG‑CGCAGACACAAAACAGCGAACAGATTTa                                                                                                                >  1:965877/1‑148 (MQ=255)
                                     ggAGGATGTGGCAAAGGTTTGCGTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACAACGGGTTTGTTTTCGAGGTCTGTTTCCACCCTCGAGTCACAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTAc                                                                                                               <  2:2700310/147‑1 (MQ=255)
                                        ggATGTGGCAAACGTTTGCTTTTCGCTTGCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGCTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGt                                                                                                            <  2:1047695/148‑1 (MQ=255)
                                          aTGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTG‑CGCAGACTCAAAAAAGCGAACTGATTTAACGTCt                                                                                                          >  2:43072/1‑148 (MQ=255)
                                             tgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGa                                                                                                       <  2:550503/148‑1 (MQ=255)
                                                 aaaGGTTTGGTTTTCTCTTTCGGTCAGATAAAATGCGCGCTTTGCTACCTCGGTTTTGTTTTCGTTGTGTGTTTCCCCCCTCGTGTCATAAACTGCGCAAGGGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGAt                                                                                                   <  1:3120223/148‑1 (MQ=255)
                                                  aaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATc                                                                                                  >  2:3329614/1‑148 (MQ=255)
                                                   aCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTTTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCg                                                                                                 <  2:734536/148‑1 (MQ=255)
                                                      tttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGGTT‑CGCAGACACAAAACACCGAACACATTTACCCTCTTGTACATCGTcc                                                                                              >  1:3223920/1‑148 (MQ=255)
                                                       ttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccg                                                                                             >  2:2590915/1‑148 (MQ=255)
                                                       ttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccg                                                                                             >  2:618281/1‑148 (MQ=255)
                                                          cTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTG‑CGCAGACACAAAAAAGCGAAATGATTTACCGTCTTGAAGATAGTccgccg                                                                                          >  1:1038107/1‑148 (MQ=255)
                                                          cTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACACCGAACAGATTTACCGTCTTGAAGATCGTccgccg                                                                                          >  1:2404792/1‑148 (MQ=255)
                                                              tctcTTTCGGTCAGATAAATTGCCCGCTTTGCTACCACGGGTTTTTTTTCGTTGTCTGTTACCACCCTCGAGTCACAAATTGCGAAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTc                                                                                      <  1:1556131/148‑1 (MQ=255)
                                                               ctgtttCGGTCAGCTAATAGGCCCGCTTTGATACCAGGGTTTTTTTTTCGATGTCTGTTACCCCCCTCTAGTCACAAATGGCGCACCGGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTcc                                                                                     <  1:260789/145‑1 (MQ=255)
                                                               ctctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCAGGGTTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAATTGCGCACCCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTcc                                                                                     <  1:3415962/148‑1 (MQ=255)
                                                               ctctTTCGGACAGATAAAAGGCCCTCTTTGCTACCTGGGTTTTGTTTTCGATGTGTGTGCCCCCCCTCGAGTCATAAATGGCCCACCGGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTcc                                                                                     <  1:1953341/148‑1 (MQ=255)
                                                                 ctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTc                                                                                   >  2:2433736/1‑148 (MQ=255)
                                                                   ttCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCaa                                                                                 >  1:531655/1‑148 (MQ=255)
                                                                    tCGGTCAGCTAAAATGCCAGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGCGTG‑GGCAGAAACAAAACAACGCACAGATTTTCCGTCTTGTATTTGGGCCGCCGCCTACTCaaa                                                                                >  1:1504259/1‑148 (MQ=255)
                                                                      ggTCAGCTAAATGGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAcc                                                                              <  2:1528080/148‑1 (MQ=255)
                                                                       gTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAccc                                                                             >  2:129437/1‑148 (MQ=255)
                                                                          aGCTAAACGGCCCGGGTTGCTACCTGGGGTTTTTTTTCGTTGTGTGTCTCCACCCTCGCTTCATAAACTGCGCAACGGGTGC‑CCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGt                                                                          <  1:2658029/148‑1 (MQ=255)
                                                                          aGCTAAAATGCCCGCTTTGCTACCACGGTATTGTTTTCGATGTCTGTTTCCACCCTCGAGACAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGt                                                                          <  2:3482304/148‑1 (MQ=255)
                                                                          aGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGt                                                                          <  2:2380564/148‑1 (MQ=255)
                                                                              aaaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgc                                                                      >  1:3394975/1‑148 (MQ=255)
                                                                              aaaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTTAATATCGTCCGCCGCTTCCTCAAAACCGGTTTgg                                                                      >  1:1841456/1‑147 (MQ=255)
                                                                               aaaTGCCCGCTTTGCTAGCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTG‑CGCAGACGAAAAAAAGCCAACAGATTTAACGTCTTGAAGATCGTCCGCCCCGTCCAAAAACCCGGTTTgcc                                                                     >  1:1747632/1‑148 (MQ=255)
                                                                               aaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAAAAGCGAACAGATTTACCGTCTTGAAGATAGTCCGCCGCTTCCACAAACCCAGTTTgcc                                                                     >  1:3355720/1‑148 (MQ=255)
                                                                                 aTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCTAGTCAGAAAATGCGCAACCGTTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgc                                                                   <  2:3125110/148‑1 (MQ=255)
                                                                                 aTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCTGTTTgccgc                                                                   >  2:3356855/1‑148 (MQ=255)
                                                                                     ccGCTTTGCTACCCGGGTTTTTTTTTCGTTGTCTTTTGCCACCCTCGACTCACAAAATGCGCACCGGGTGG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGt                                                               <  1:2744760/148‑1 (MQ=255)
                                                                                     ccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑TGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGt                                                               >  2:2813216/1‑148 (MQ=255)
                                                                                        cTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTG‑CGCCGAATCAAAACACCGCACAGATTTAACGTCTTGAAGATAGTCCGCCCCGTCCACAAAACCAGTTTTCCGCCTGTCAg                                                            >  1:784935/1‑148 (MQ=255)
                                                                                         tttGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTG‑CGCAGACTAAAAAAAACCAACAGATTTAACCACTTTAAGATAGTCAGCCGCTGCCACAAAACCAGTTTTGCGCCTGTCAGc                                                           >  1:1780177/1‑148 (MQ=255)
                                                                                           tGCTACGACGGGATTGTTTTCTATGTCTGTTTCCACCCTCGTGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCAt                                                         <  2:2682824/148‑1 (MQ=255)
                                                                                           tGCTACCTCTGGTTTTTTTTCGTTGTTTGTCCCCACCCACTAGACATCAACGGCCCACCCGCGCC‑CCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCAt                                                         <  1:43072/148‑1 (MQ=255)
                                                                                            gCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTG‑CGCAGACTCAAAAAAGCGAACTGATTTAACGTCTTGAAGATAGTCCGCCGCTTCCTCAAAACCAGTTTTGCGCCGGTCAGCAta                                                        >  1:87919/1‑147 (MQ=255)
                                                                                               aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgc                                                     >  2:3169811/1‑148 (MQ=255)
                                                                                               aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgc                                                     >  2:480615/1‑148 (MQ=255)
                                                                                                       aTTTTTTTCGATTTCTGTTTCCACCCTCGAGTCAGAACAGGCGCCCCCGGTTG‑CCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATg                                             <  1:1839394/148‑1 (MQ=255)
                                                                                                       aTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTG‑CGCAGACTAAAAAAATCGAACAGAATTACCGTCTTGAAGATCGTCCGCCGCGTGCGCAAAACCAGTTTGCCGCCAGTCAGCAAAAGATGGCGATg                                             >  1:3038209/1‑148 (MQ=255)
                                                                                                        ttGTTTTCGTTGTCTGTTTCCACCCTCGAGACATAAAAGGCGCACCCGGTGC‑CCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGt                                            <  1:3621293/148‑1 (MQ=255)
                                                                                                        ttGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAAAAGCGAACTGATTTAACGGCTTTAAGATCGGCCGCCCCTTACTCAAAACCAGGTTGCCGCCAGTCAGCATCTGCTGGCGATGt                                            >  1:3288124/1‑148 (MQ=255)
                                                                                                         tGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCCGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCGCAAACCCGGGTTTCCGCCAGTCAGCAACTGCTGGCGATGtt                                           >  1:3485668/1‑148 (MQ=255)
                                                                                                         tGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATAGTCCGCCGCTTACTCAAACCCTGTTTGGCGCCGGTCAGCATCTGCTGGCGATGtt                                           >  2:1932639/1‑148 (MQ=255)
                                                                                                           ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCg                                         <  2:758071/148‑1 (MQ=255)
                                                                                                              tCGATGTCTGTTTCCCCCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTg                                      <  2:2638456/148‑1 (MQ=255)
                                                                                                                    tctttgtCCCCCCCCGATTCACAAAATGCGCAA‑CGGTTGCCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                <  1:3802511/142‑1 (MQ=255)
                                                                                                                    tCTGTTTCCCCCCTCGAGTCAGAAAAGGCGCACCCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                <  2:1670465/148‑1 (MQ=255)
                                                                                                                    tCTGTTTCCACCCGCGAGTCATAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                <  2:3223945/148‑1 (MQ=255)
                                                                                                                    tCTGTTTCCACCCGCGAGTCAGAAAATGCGCACCCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCACACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                <  2:1670424/148‑1 (MQ=255)
                                                                                                                     cggTTACCCCCCTCGAGACACAAACTGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                               <  1:934851/146‑1 (MQ=255)
                                                                                                                       gTTTCCCCCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCa                             <  2:2923436/148‑1 (MQ=255)
                                                                                                                            cACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACAGATTTAACGTCGTTAAGATAGTCCGCCGCTTCCACAAAACCAGTTTTGCGCCGGTCAGCATCTGGTGGCGATGTTCGTTTCGGTGTTAACGCCa                        >  1:596737/1‑148 (MQ=255)
                                                                                                                                  cGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTa                  <  1:618281/148‑1 (MQ=255)
                                                                                                                                  cGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGAAACAAAACAGCCAACTGAATTACCGTCTAGAACAACCTACGCCGCAACCTCAAAACCGGTTTGCCGCCTATCAGCAACAGCTGGCGATCTTCGTTTCGGTTATCACGCCACCCCTa                  >  2:831172/1‑148 (MQ=255)
                                                                                                                                     gacccaaaaGGCGCACCGGGTGG‑CCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTa               <  1:2294765/143‑1 (MQ=255)
                                                                                                                                        aGAAAATGCGCAACCGGTTG‑CGCAGACACAAAAAAGCGAACTGATTTAACCTCTTTAATATAGTCCGCCCCGTCCACAAAACCAGTTTGGCGCCTGTCAGCATCTGCTGGCGATTTTCGTTGCGGTGATCACGCCAGCGCTATTAATc            >  1:2085849/1‑148 (MQ=255)
                                                                                                                                           ataggcgcAACGGGTGG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGc         <  1:2759776/144‑1 (MQ=255)
                                                                                                                                                 gcAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcg   <  2:1507929/148‑1 (MQ=255)
                                                                                                                                                  cAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcgc  <  2:2847756/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGC  >  CP009273/3822192‑3822485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑