Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,822,340 A→C 13.4% Q12H (CAA→CAC) ‡ xanP → xanthine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,822,3400AC13.4% 348.7 / 15.9 205Q12H (CAA→CAC) ‡xanPxanthine permease
Reads supporting (aligned to +/- strand):  ref base A (107/65);  new base C (6/26);  total (113/92)
Fisher's exact test for biased strand distribution p-value = 9.31e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 210 total aligned reads displayed.

ATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTG  >  CP009273/3822193‑3822487
                                                                                                                                                   |                                                                                                                                                    
ataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCaa                                                                                                                                                      <  1:1085529/148‑1 (MQ=255)
  aaaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAcc                                                                                                                                                    >  1:758071/1‑148 (MQ=255)
  aaaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTACACCCTCGAGTCAGAAAATGCGCAAcc                                                                                                                                                    >  1:734547/1‑148 (MQ=255)
    aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCgg                                                                                                                                                  >  1:3005906/1‑148 (MQ=255)
     aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGCTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCAccccgg                                                                                                                                                 >  1:1314519/1‑145 (MQ=255)
     aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGt                                                                                                                                                 <  1:2763790/148‑1 (MQ=255)
     aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGt                                                                                                                                                 <  1:3359638/148‑1 (MQ=255)
      aaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGtt                                                                                                                                                >  2:1523032/1‑148 (MQ=255)
      aaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTATCTTTCGGTCAGCTAAAATGCCCGCTTTTCTAACACTGGATTGTTTTCTATGTCTGTTTACACCCTCGAGTCAGAAAATGCGCAACAGGtt                                                                                                                                                >  2:2553560/1‑148 (MQ=255)
          gCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTg‑tgc                                                                                                                                           >  1:1984011/1‑148 (MQ=255)
           cATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCa                                                                                                                                          >  2:2100687/1‑148 (MQ=255)
            aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCCCCCTCGAGTCAGAAAATGCGCAACCGGTGT‑CGCAg                                                                                                                                         >  2:2142453/1‑148 (MQ=255)
              ttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGTTG‑CGCAGAc                                                                                                                                       >  1:2847756/1‑148 (MQ=255)
              ttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTG‑CGCAGAc                                                                                                                                       >  2:2832630/1‑148 (MQ=255)
              ttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGAc                                                                                                                                       >  2:3621293/1‑148 (MQ=255)
               tATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACt                                                                                                                                      <  2:382780/148‑1 (MQ=255)
                aTCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTc                                                                                                                                     >  2:117302/1‑148 (MQ=255)
                 tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCa                                                                                                                                    >  2:2939606/1‑148 (MQ=255)
                      tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACa                                                                                                                               >  1:1105912/1‑148 (MQ=255)
                       ttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTG‑CGCAGACACaaaaaat                                                                                                                              >  1:1259510/1‑145 (MQ=255)
                         aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCGGGTTG‑CGCAGACTCAAAACAGCg                                                                                                                            <  1:3143226/148‑1 (MQ=255)
                         aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAACCGTTG‑CGCAGACTCAAAACatcc                                                                                                                            >  1:2441273/1‑145 (MQ=255)
                          aaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGAGTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTG‑CGCAGACTCAAAACAGCGa                                                                                                                           >  2:3605961/1‑148 (MQ=255)
                           atatTTAGATGAGTATGTGGCAAACTTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTTTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGaa                                                                                                                          <  2:3105638/148‑1 (MQ=255)
                           atatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGTCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGaa                                                                                                                          >  2:2812260/1‑148 (MQ=255)
                           atatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGaa                                                                                                                          >  2:2759646/1‑148 (MQ=255)
                            tatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAACCGTTT‑CGCAGACACAAAAAAACGaaa                                                                                                                         >  1:796164/1‑147 (MQ=255)
                             atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGacca                                                                                                                        >  2:1753084/1‑145 (MQ=255)
                             atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCCTTG‑CGCAGACTCAAAACacccaaaa                                                                                                                        >  1:718440/1‑141 (MQ=255)
                              tttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTG‑CGCAGACTCAAAAAAACGAAATg                                                                                                                       >  1:1608597/1‑148 (MQ=255)
                                 aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGAtt                                                                                                                    >  2:401841/1‑148 (MQ=255)
                                 aGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGAtt                                                                                                                    >  2:483238/1‑148 (MQ=255)
                                   ttgtgtttgtgCCAAAGGTTTGGTTTTCTGTTTCGGTCAGATAAAATGCCCGCTTTGCTACCAGGGTTTTGTTTTCGTTGTGTGTGTCCACCCACGAGGCATAAAATGCGCACCCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTa                                                                                                                  <  1:2203219/141‑1 (MQ=255)
                                   aTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAATTGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTa                                                                                                                  <  2:2874652/148‑1 (MQ=255)
                                   aTGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTG‑CGCAGAATAAAAAAAACGAAATGATTTa                                                                                                                  >  1:965312/1‑148 (MQ=255)
                                       ggttgtgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGt                                                                                                              <  2:1047710/145‑1 (MQ=255)
                                       ggATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGt                                                                                                              <  2:1669051/148‑1 (MQ=255)
                                        gATGTGGCAAACGTTTGCTTTTCTCTTTCGGACAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCCCCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTc                                                                                                             <  2:3345883/148‑1 (MQ=255)
                                         aTGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTG‑CGCAGACTCAAAAAAGCGAACTGATTTAACGTCt                                                                                                            >  2:43072/1‑148 (MQ=255)
                                         aTGTGGCAAACGTTTGCTTTTCGCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCt                                                                                                            <  2:2165294/148‑1 (MQ=255)
                                            tgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGa                                                                                                         <  2:550503/148‑1 (MQ=255)
                                                aaaGGTTTGGTTTTCTCTTTCGGTCAGATAAAATGCGCGCTTTGCTACCTCGGTTTTGTTTTCGTTGTGTGTTTCCCCCCTCGTGTCATAAACTGCGCAAGGGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGAt                                                                                                     <  1:3120223/148‑1 (MQ=255)
                                                  aCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTTTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCg                                                                                                   <  2:734536/148‑1 (MQ=255)
                                                     tttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGGTT‑CGCAGACACAAAACACCGAACACATTTACCCTCTTGTACATCGTcc                                                                                                >  1:3223920/1‑148 (MQ=255)
                                                      ttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccg                                                                                               >  2:2234863/1‑148 (MQ=255)
                                                      ttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccg                                                                                               >  2:618281/1‑148 (MQ=255)
                                                      ttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccg                                                                                               >  2:2590915/1‑148 (MQ=255)
                                                         cTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTG‑CGCAGACACAAAAAAGCGAAATGATTTACCGTCTTGAAGATAGTccgccg                                                                                            >  1:1038107/1‑148 (MQ=255)
                                                         cTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACACCGAACAGATTTACCGTCTTGAAGATCGTccgccg                                                                                            >  1:2404792/1‑148 (MQ=255)
                                                             tctcTTTCGGTCAGATAAATTGCCCGCTTTGCTACCACGGGTTTTTTTTCGTTGTCTGTTACCACCCTCGAGTCACAAATTGCGAAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTc                                                                                        <  1:1556131/148‑1 (MQ=255)
                                                              ctgtttCGGTCAGCTAATAGGCCCGCTTTGATACCAGGGTTTTTTTTTCGATGTCTGTTACCCCCCTCTAGTCACAAATGGCGCACCGGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTcc                                                                                       <  1:260789/145‑1 (MQ=255)
                                                              ctctTTCGGTCAGTAAACCGGCCCGCTTTGCTACCAGGGTTTTGTTTTGGTTGTCTTTTTCCACCCTCGAGTCAGAAATTGCGCAACGGGTGG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTcc                                                                                       <  1:3415976/148‑1 (MQ=255)
                                                              ctctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTcc                                                                                       >  1:1922085/1‑148 (MQ=255)
                                                              ctctTTCGGTCAGCTAAAAGGCCCGCTTTGCTACCAGGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTcc                                                                                       <  2:1375215/148‑1 (MQ=255)
                                                                ctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTc                                                                                     >  2:2433736/1‑148 (MQ=255)
                                                                   tCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCaaa                                                                                  >  1:3403996/1‑148 (MQ=255)
                                                                   tCGGTCAGCTAAAATGCCAGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGCGTG‑GGCAGAAACAAAACAACGCACAGATTTTCCGTCTTGTATTTGGGCCGCCGCCTACTCaaa                                                                                  >  1:1504259/1‑148 (MQ=255)
                                                                         agatAAAATGCCCGATTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGt                                                                            <  2:3482350/145‑1 (MQ=255)
                                                                         aGCTAAACGGCCCGGGTTGCTACCTGGGGTTTTTTTTCGTTGTGTGTCTCCACCCTCGCTTCATAAACTGCGCAACGGGTGC‑CCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGt                                                                            <  1:2658029/148‑1 (MQ=255)
                                                                         aGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGt                                                                            <  2:2380564/148‑1 (MQ=255)
                                                                         aGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGt                                                                            <  2:3482300/148‑1 (MQ=255)
                                                                          ccTAAAATGCCCGTTTTGCCACCTGGGGTTTTTTTTCTTTGTGTTTTACCACCCTCGAGTAACAAAAGGCGCAACCGGTGG‑CCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGtt                                                                           <  1:583452/147‑1 (MQ=255)
                                                                             aaaaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTG‑CGCAGACTCAAAACACCGAACTGATTTACCGTGTTGTAGATCGTCCGCCGCTTCCACAAAACCTGgtttc                                                                        >  1:1841424/1‑146 (MQ=255)
                                                                              aaaTGCCCGCTTTGCTAGCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGGTG‑CGCAGACGAAAAAAAGCCAACAGATTTAACGTCTTGAAGATCGTCCGCCCCGTCCAAAAACCCGGTTTgcc                                                                       >  1:1747632/1‑148 (MQ=255)
                                                                                aTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgc                                                                     <  2:3125218/148‑1 (MQ=255)
                                                                                    ccGTTTTGCTACCACGGTTTTGTTTTCGATGTCTTTTTCCACCCTCGAGTCATAAAATGCGCAAGGGGTGG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGt                                                                 <  1:274592/148‑1 (MQ=255)
                                                                                    ccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑TGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGt                                                                 >  2:1002984/1‑148 (MQ=255)
                                                                                        tttGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTG‑CGCAGACTAAAAAAAACCAACAGATTTAACCACTTTAAGATAGTCAGCCGCTGCCACAAAACCAGTTTTGCGCCTGTCAGc                                                             >  1:1780177/1‑148 (MQ=255)
                                                                                          tGCTACCTCTGGTTTTTTTTCGTTGTTTGTCCCCACCCACTAGACATCAACGGCCCACCCGCGCC‑CCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCAt                                                           <  1:43072/148‑1 (MQ=255)
                                                                                              aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgc                                                       >  2:480615/1‑148 (MQ=255)
                                                                                               ccACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgct                                                      >  2:2698030/1‑148 (MQ=255)
                                                                                               ccACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCAGTTTGCCGCCAGGCAGCATctgct                                                      >  1:613551/1‑148 (MQ=255)
                                                                                                      aTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCACACTCAAAACACCGAACAGATTTAACGTCTTGAAGATAGTCCGCCGCGTCCACAAAACCAGTTTTCCGCCTGTCAGCAAATGATGGCGATg                                               >  1:3331418/1‑148 (MQ=255)
                                                                                                      aTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTG‑CGCAGACTAAAAAAATCGAACAGAATTACCGTCTTGAAGATCGTCCGCCGCGTGCGCAAAACCAGTTTGCCGCCAGTCAGCAAAAGATGGCGATg                                               >  1:3038209/1‑148 (MQ=255)
                                                                                                        tGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATAGTCCGCCGCTTACTCAAACCCTGTTTGGCGCCGGTCAGCATCTGCTGGCGATGtt                                             >  2:1932639/1‑148 (MQ=255)
                                                                                                        tGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGtt                                             >  1:3486925/1‑148 (MQ=255)
                                                                                                          ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCg                                           <  2:758071/148‑1 (MQ=255)
                                                                                                          ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTGG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCg                                           <  2:2673285/148‑1 (MQ=255)
                                                                                                           tttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCCACCGGTTG‑CGCAGGCTCAAAACAGCGAACGGATTTTCCCGCTTGAAGAGCGGCCGCCGCGTGCTCAAACCCAGTGTGGCGCCTGTCAGCATCTGCTGGCGCGGTTCGt                                          >  1:2719701/1‑148 (MQ=255)
                                                                                                             tCGATGTCTGTTTCCCCCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTg                                        <  2:2638456/148‑1 (MQ=255)
                                                                                                                   tctttgtCCCCCCCCGATTCACAAAATGCGCAA‑CGGTTGCCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                  <  1:3802511/142‑1 (MQ=255)
                                                                                                                   tCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                  <  2:1670464/148‑1 (MQ=255)
                                                                                                                   tCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTGG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGa                                  <  2:1670812/148‑1 (MQ=255)
                                                                                                                    cTGTTTCCACCCTCGAGTCAGAAAAGGCGCACCCGGTGG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                                 <  1:934289/148‑1 (MQ=255)
                                                                                                                      gTTTCCCCCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCa                               <  2:2923436/148‑1 (MQ=255)
                                                                                                                        ttCCACCCTCGAGTCAGAAAATGCGCAACCCGTTG‑CGCAGACTCAAAAAAGCGAACAGTTTTAACGTCATGTAGATAGTCCCCCGCTTCCTAAAAACCCGTTTGGCGCCTGTCAGCAACATCTTGCGATGTTCGTTTCGGTGATCaag                             >  1:3422781/1‑146 (MQ=255)
                                                                                                                         tCCACCCTCGAGTCAGAAATGGCGCACCCGGTGG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGc                            <  1:3754683/148‑1 (MQ=255)
                                                                                                                           cACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCa                          >  2:3358298/1‑148 (MQ=255)
                                                                                                                            aCCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAg                         >  2:1552315/1‑148 (MQ=255)
                                                                                                                                 cGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTa                    >  2:3245545/1‑148 (MQ=255)
                                                                                                                                 cGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGAAACAAAACAGCCAACTGAATTACCGTCTAGAACAACCTACGCCGCAACCTCAAAACCGGTTTGCCGCCTATCAGCAACAGCTGGCGATCTTCGTTTCGGTTATCACGCCACCCCTa                    >  2:831172/1‑148 (MQ=255)
                                                                                                                                    gacccaaaaGGCGCACCGGGTGG‑CCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTa                 <  1:2294765/143‑1 (MQ=255)
                                                                                                                                       aGAAAATGCGCAACCGGTTG‑CGCAGACACAAAAAAGCGAACTGATTTAACCTCTTTAATATAGTCCGCCCCGTCCACAAAACCAGTTTGGCGCCTGTCAGCATCTGCTGGCGATTTTCGTTGCGGTGATCACGCCAGCGCTATTAATc              >  1:2085849/1‑148 (MQ=255)
                                                                                                                                         aaatTGCGCACCCGGTTC‑CCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg            <  1:346812/148‑1 (MQ=255)
                                                                                                                                          aacggcgcATCGGGTGG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGc           <  1:2759646/144‑1 (MQ=255)
                                                                                                                                           aaTGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc          <  2:1192541/148‑1 (MQ=255)
                                                                                                                                                gcAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcg     <  2:3567627/148‑1 (MQ=255)
                                                                                                                                                 cAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcgc    <  2:2847756/148‑1 (MQ=255)
                                                                                                                                                 cAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcgc    <  2:3202571/148‑1 (MQ=255)
                                                                                                                                                   aCCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGATGGCGATCTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTg  >  2:1505294/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTG‑CGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTG  >  CP009273/3822193‑3822487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑