Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,830,819 T→G 100% F169L (TTT→TTG)  setC → putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,830,8190TG97.6% 1032.6 / 3.0 258F169L (TTT→TTG) setCputative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base T (3/4);  new base G (128/123);  total (131/127)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 258 total aligned reads displayed.

CAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAGCGG  >  CP009273/3830674‑3830963
                                                                                                                                                 |                                                                                                                                                
 aaaCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGt                                                                                                                                               <  1:730986/148‑1 (MQ=255)
  aaCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCTACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGtt                                                                                                                                              <  2:2782489/148‑1 (MQ=255)
  aaCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGtt                                                                                                                                              <  1:1569202/148‑1 (MQ=255)
  aaCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGtt                                                                                                                                              >  1:1439279/1‑148 (MQ=255)
   aCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGttt                                                                                                                                             <  1:3731751/148‑1 (MQ=255)
   aCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGttt                                                                                                                                             >  1:3023304/1‑148 (MQ=255)
   aCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGttt                                                                                                                                             <  1:3224600/148‑1 (MQ=255)
      cGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTaa                                                                                                                                          <  2:2028794/148‑1 (MQ=255)
         aaaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGt                                                                                                                                       <  2:235964/148‑1 (MQ=255)
          aaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTg                                                                                                                                      <  1:1419175/148‑1 (MQ=255)
          aaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTg                                                                                                                                      <  1:213317/148‑1 (MQ=255)
           aTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGa                                                                                                                                     <  2:1072246/148‑1 (MQ=255)
            tGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCTCTTATGAACTGGAAATGGGATTGAGTTTTAAAGTGAt                                                                                                                                    >  1:2620293/1‑148 (MQ=255)
            tGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGAt                                                                                                                                    <  1:3627729/148‑1 (MQ=255)
             gTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATg                                                                                                                                   >  1:3505189/1‑148 (MQ=255)
              ttCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGt                                                                                                                                  <  2:3714411/148‑1 (MQ=255)
                cgccctcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTAt                                                                                                                                <  2:576691/148‑1 (MQ=255)
                    ctcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCa                                                                                                                            >  2:644213/1‑148 (MQ=255)
                      cgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCAcc                                                                                                                          >  1:3186420/1‑148 (MQ=255)
                          cGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTg                                                                                                                      <  1:1536887/148‑1 (MQ=255)
                             gAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCa                                                                                                                   <  2:31316/148‑1 (MQ=255)
                             gAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCa                                                                                                                   >  2:1264360/1‑148 (MQ=255)
                                  cGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCa                                                                                                              <  2:1559942/148‑1 (MQ=255)
                                  cGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCGTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCa                                                                                                              <  2:73496/148‑1 (MQ=255)
                                      gACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATttg                                                                                                          >  2:3798388/1‑148 (MQ=255)
                                       aCAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATttgt                                                                                                         >  1:1847571/1‑148 (MQ=255)
                                       aCAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATttgt                                                                                                         <  1:2250129/148‑1 (MQ=255)
                                        cAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGTTCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATttgtt                                                                                                        <  1:296227/148‑1 (MQ=255)
                                          gAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTtgttgt                                                                                                      <  1:206948/148‑1 (MQ=255)
                                            aCAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGttt                                                                                                    >  2:1866208/1‑148 (MQ=255)
                                             cAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTg                                                                                                   >  2:915949/1‑148 (MQ=255)
                                                 ccGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGa                                                                                               <  1:1918057/148‑1 (MQ=255)
                                                 ccGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGa                                                                                               <  2:889023/148‑1 (MQ=255)
                                                      agaCGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGAt                                                                                          >  2:2101158/1‑148 (MQ=255)
                                                      agaCGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGAt                                                                                          >  1:2794348/1‑148 (MQ=255)
                                                      agaCGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTGATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGAt                                                                                          <  2:2718884/148‑1 (MQ=255)
                                                       gaCGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAATGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGAGGACTGATa                                                                                         >  2:3396942/1‑148 (MQ=255)
                                                          ggTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTc                                                                                      <  1:352936/148‑1 (MQ=255)
                                                            tCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTg                                                                                    >  2:3798154/1‑148 (MQ=255)
                                                             cATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTgg                                                                                   >  2:1375930/1‑148 (MQ=255)
                                                             cATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTgg                                                                                   >  1:778591/1‑148 (MQ=255)
                                                              aTGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGgt                                                                                  >  2:2496718/1‑148 (MQ=255)
                                                                gTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGgttg                                                                                <  2:1117785/148‑1 (MQ=255)
                                                                 ttCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGgttgt                                                                               <  1:1141235/148‑1 (MQ=255)
                                                                  tCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGgttgtt                                                                              >  1:673837/1‑148 (MQ=255)
                                                                     gTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACAGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGttttt                                                                           >  1:2378689/1‑148 (MQ=255)
                                                                       aCATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGc                                                                         >  1:2574760/1‑148 (MQ=255)
                                                                          tttttACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCAt                                                                      <  2:3663818/148‑1 (MQ=255)
                                                                            tttACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCa                                                                    <  1:1740186/148‑1 (MQ=255)
                                                                             ttACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGAGTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCaa                                                                   >  2:2302103/1‑148 (MQ=255)
                                                                              tACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAAt                                                                  <  2:720699/148‑1 (MQ=255)
                                                                              tACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAAt                                                                  <  1:3590659/148‑1 (MQ=255)
                                                                                cGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATAc                                                                >  2:3769231/1‑148 (MQ=255)
                                                                                 gTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACa                                                               <  1:3425411/148‑1 (MQ=255)
                                                                                  tGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACaa                                                              <  2:1927610/148‑1 (MQ=255)
                                                                                  tGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACaa                                                              >  1:2966045/1‑148 (MQ=255)
                                                                                  tGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACaa                                                              <  1:2894307/148‑1 (MQ=255)
                                                                                       aGATCTCGCGTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGaaa                                                         <  1:400849/148‑1 (MQ=255)
                                                                                        gATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAat                                                        <  2:946096/148‑1 (MQ=255)
                                                                                        gATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAat                                                        <  2:1252017/148‑1 (MQ=255)
                                                                                        gATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCACACCGCTGCCATCGCATTAGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATAAAAAGAAat                                                        >  2:1898400/1‑148 (MQ=255)
                                                                                         aTCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAata                                                       <  2:3505189/148‑1 (MQ=255)
                                                                                         aTCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAata                                                       >  1:849416/1‑148 (MQ=255)
                                                                                         aTCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAata                                                       >  2:1356664/1‑148 (MQ=255)
                                                                                          tctcGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAatat                                                      >  1:332343/1‑148 (MQ=255)
                                                                                            tcGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTc                                                    <  2:3186674/148‑1 (MQ=255)
                                                                                            tcGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTc                                                    <  1:1944728/148‑1 (MQ=255)
                                                                                            tcGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTc                                                    <  1:627027/148‑1 (MQ=255)
                                                                                            tcGCGTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTc                                                    <  2:1647887/148‑1 (MQ=255)
                                                                                             cGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTcc                                                   <  2:2230167/148‑1 (MQ=255)
                                                                                             cGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTcc                                                   <  1:1386915/148‑1 (MQ=255)
                                                                                             cGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGACAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTcc                                                   <  2:2158795/148‑1 (MQ=255)
                                                                                              gCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCt                                                  <  2:3249383/148‑1 (MQ=255)
                                                                                               cTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTg                                                 <  2:539055/148‑1 (MQ=255)
                                                                                                ttGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGt                                                <  2:3345106/148‑1 (MQ=255)
                                                                                                ttGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGt                                                <  1:546292/148‑1 (MQ=255)
                                                                                                  gCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCg                                              <  2:3017992/148‑1 (MQ=255)
                                                                                                   ccTGGGTTATCGGGCCACCTCTCGCTTAAGAACTTTCAATGGTATTGAGTATTAAAGACATGTATCTCACCGCAGACATCGCATTTGTTGTTATAGTACTGATAGTCTGTTTGTTTTATCCATCAATACAAATACATATTCCTCTCGt                                             >  1:3068298/1‑148 (MQ=255)
                                                                                                   ccTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGt                                             >  1:479758/1‑148 (MQ=255)
                                                                                                     tGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTa                                           <  1:3468411/148‑1 (MQ=255)
                                                                                                      gggTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTAc                                          >  1:1213053/1‑148 (MQ=255)
                                                                                                       ggTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTAcc                                         >  1:2178482/1‑148 (MQ=255)
                                                                                                          tATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCaa                                      >  1:2655490/1‑148 (MQ=255)
                                                                                                           aTCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAAc                                     <  2:395678/148‑1 (MQ=255)
                                                                                                            tCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAAcc                                    >  2:3579572/1‑148 (MQ=255)
                                                                                                              gggCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCg                                  <  1:903363/148‑1 (MQ=255)
                                                                                                               ggCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGt                                 >  1:1044565/1‑148 (MQ=255)
                                                                                                               ggCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGt                                 >  1:1046541/1‑148 (MQ=255)
                                                                                                                     cgctcgctTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAAt                           >  1:1749139/1‑148 (MQ=255)
                                                                                                                         cgctTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTa                       >  2:240645/1‑148 (MQ=255)
                                                                                                                           ctTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTAcc                     >  1:2099264/1‑148 (MQ=255)
                                                                                                                              aTGAACGTGGAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACACTACCCGTAGAAATTTTACCCTc                  <  2:2587503/148‑1 (MQ=255)
                                                                                                                                 aaCTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTATTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTccac               >  1:1676591/1‑148 (MQ=255)
                                                                                                                                 aaCTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTATTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTccac               >  1:1676520/1‑148 (MQ=255)
                                                                                                                                  aCTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTccacc              <  1:3123057/148‑1 (MQ=255)
                                                                                                                                   cTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATAGCATTTGTTGTGTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAATAAATATTCCTTTCGTTAACCAACCCGAAGAAATTATAACCTccacca             >  1:3790407/1‑147 (MQ=255)
                                                                                                                                          tGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGaa      >  1:2785951/1‑148 (MQ=255)
                                                                                                                                          tGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACACTCCACCCACAGGaa      >  2:3233822/1‑148 (MQ=255)
                                                                                                                                            ggATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAGc    >  1:2457277/1‑148 (MQ=255)
                                                                                                                                             gATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAGCg   >  1:3672482/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAGCGG  >  CP009273/3830674‑3830963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑