Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,865,863 T→A 100% T166S (ACC→TCC)  dgoD ← galactonate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,865,8630TA99.1% 689.7 / ‑2.4 209T166S (ACC→TCC) dgoDgalactonate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base A (102/105);  total (104/105)
Fisher's exact test for biased strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 210 total aligned reads displayed.

GCCAGCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATCGCCGCCAACCCAACT  >  CP009273/3865718‑3866007
                                                                                                                                                 |                                                                                                                                                
gCCAGCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGgagt                                                                                                                                                <  1:1383595/148‑1 (MQ=255)
 ccAGCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGtt                                                                                                                                               >  2:1096287/1‑148 (MQ=255)
 ccAGCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGtt                                                                                                                                               >  2:297144/1‑148 (MQ=255)
     cACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAAcc                                                                                                                                           >  2:3078075/1‑148 (MQ=255)
      aCCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAAccg                                                                                                                                          <  1:3289245/148‑1 (MQ=255)
       ccGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAAccgc                                                                                                                                         >  2:3720417/1‑148 (MQ=255)
       ccGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAAccgc                                                                                                                                         >  2:1830042/1‑148 (MQ=255)
            tcctcAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCAt                                                                                                                                    <  1:1558989/148‑1 (MQ=255)
             cctcAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATc                                                                                                                                   >  1:3107559/1‑148 (MQ=255)
               tcAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTa                                                                                                                                 <  1:1267854/148‑1 (MQ=255)
                cAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTAc                                                                                                                                <  2:3390167/148‑1 (MQ=255)
                  aTAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTCCcg                                                                                                                              >  2:2672521/1‑148 (MQ=255)
                    aaaCAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACcgcg                                                                                                                            <  2:2855863/148‑1 (MQ=255)
                      aCAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACcgcgcg                                                                                                                          >  1:1604180/1‑148 (MQ=255)
                      aCAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACcgcgcg                                                                                                                          >  1:376679/1‑148 (MQ=255)
                       cAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCgg                                                                                                                         >  2:1790937/1‑148 (MQ=255)
                           gggCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGt                                                                                                                     <  2:376679/148‑1 (MQ=255)
                            ggCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGtt                                                                                                                    <  1:2145830/148‑1 (MQ=255)
                              cGATACGGCTCCAGTTCTTTAATCAGCACTTTCTCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAAATCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTAt                                                                                                                  <  2:3601350/148‑1 (MQ=255)
                                aTACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCa                                                                                                                >  1:2440026/1‑148 (MQ=255)
                                 tACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCaa                                                                                                               >  2:2229955/1‑148 (MQ=255)
                                 tACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCaa                                                                                                               <  1:930343/148‑1 (MQ=255)
                                  aCGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAAt                                                                                                              >  1:2945877/1‑148 (MQ=255)
                                    ggCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTa                                                                                                            <  1:75256/148‑1 (MQ=255)
                                        ccAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGccc                                                                                                        <  1:2699346/148‑1 (MQ=255)
                                            ttCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGt                                                                                                    <  1:1962336/148‑1 (MQ=255)
                                             tCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGtt                                                                                                   <  2:1283357/148‑1 (MQ=255)
                                              cTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGttc                                                                                                  <  2:138650/148‑1 (MQ=255)
                                               tttAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGttct                                                                                                 <  1:1790971/148‑1 (MQ=255)
                                               tttAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGttct                                                                                                 <  1:2607923/148‑1 (MQ=255)
                                                   aTCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTcac                                                                                             >  2:1944445/1‑148 (MQ=255)
                                                   aTCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTcac                                                                                             >  1:2721581/1‑148 (MQ=255)
                                                   aTCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTcac                                                                                             >  2:3218975/1‑148 (MQ=255)
                                                   aTCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTcac                                                                                             >  1:2305842/1‑148 (MQ=255)
                                                    tCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTcaca                                                                                            <  2:519526/148‑1 (MQ=255)
                                                     cAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACaa                                                                                           <  2:2739016/148‑1 (MQ=255)
                                                        cACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCg                                                                                        <  1:3095883/148‑1 (MQ=255)
                                                        cACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCg                                                                                        <  1:357935/148‑1 (MQ=255)
                                                          cTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGtt                                                                                      >  1:1238655/1‑148 (MQ=255)
                                                            ttCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCa                                                                                    >  2:1649134/1‑148 (MQ=255)
                                                            ttCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCa                                                                                    <  1:137294/148‑1 (MQ=255)
                                                              cGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGt                                                                                  <  2:2929851/148‑1 (MQ=255)
                                                              cGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGt                                                                                  <  1:1086873/148‑1 (MQ=255)
                                                              cGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGt                                                                                  <  1:3421373/148‑1 (MQ=255)
                                                               gCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGtt                                                                                 <  1:1455398/148‑1 (MQ=255)
                                                                ccATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGttt                                                                                <  2:2252723/148‑1 (MQ=255)
                                                                    cGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAg                                                                            <  1:2931486/148‑1 (MQ=255)
                                                                      gcgcgcTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGt                                                                          <  1:249587/148‑1 (MQ=255)
                                                                        gcgcTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTAt                                                                        <  1:2894720/148‑1 (MQ=255)
                                                                        gcgcTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTAt                                                                        <  1:2894484/148‑1 (MQ=255)
                                                                             gACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAg                                                                   <  2:38921/148‑1 (MQ=255)
                                                                             gACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAg                                                                   >  1:1849690/1‑148 (MQ=255)
                                                                              aCGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGc                                                                  >  1:3067622/1‑148 (MQ=255)
                                                                                 cgACCGTGGAAATCAAGACCAAACTCAATCTGATAGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGa                                                               <  2:3561060/148‑1 (MQ=255)
                                                                                    ccGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGAttt                                                            <  2:3271791/148‑1 (MQ=255)
                                                                                    ccGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGAttt                                                            <  1:705789/148‑1 (MQ=255)
                                                                                     cctGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTc                                                           >  2:3493156/3‑148 (MQ=255)
                                                                                       tGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTcgc                                                         <  2:2723346/148‑1 (MQ=255)
                                                                                            aTCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGc                                                    >  2:673411/1‑148 (MQ=255)
                                                                                             tCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCg                                                   <  2:3030911/148‑1 (MQ=255)
                                                                                             tCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCg                                                   >  2:562482/1‑148 (MQ=255)
                                                                                             tCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCg                                                   >  2:2483209/1‑148 (MQ=255)
                                                                                             tCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCg                                                   >  2:903390/1‑148 (MQ=255)
                                                                                              cAACACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCAACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGACGACGAGTTAGCGTAGAGt                                                  >  2:962395/1‑148 (MQ=255)
                                                                                                  aCCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTa                                              <  2:1749739/148‑1 (MQ=255)
                                                                                                   ccAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTaa                                             <  2:1902089/148‑1 (MQ=255)
                                                                                                   ccAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTaa                                             <  1:305748/148‑1 (MQ=255)
                                                                                                   ccAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTaa                                             <  1:305978/148‑1 (MQ=255)
                                                                                                      aaCTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGc                                          >  2:245093/1‑148 (MQ=255)
                                                                                                      aaCTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGc                                          >  2:1784383/1‑148 (MQ=255)
                                                                                                      aaCTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGc                                          <  1:3720417/148‑1 (MQ=255)
                                                                                                      aaCTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGc                                          >  1:903732/1‑148 (MQ=255)
                                                                                                      aaCTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGc                                          <  1:1830042/148‑1 (MQ=255)
                                                                                                         tCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTGGAAGGTCTCGAAGCCGAGTTCGCGTCGCGGTTTAATGCCgg                                       >  1:2262176/1‑147 (MQ=255)
                                                                                                          cAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTc                                      >  1:3560227/1‑148 (MQ=255)
                                                                                                             tCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGAt                                   >  1:1666837/1‑148 (MQ=255)
                                                                                                             tCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGAt                                   >  2:1219983/1‑148 (MQ=255)
                                                                                                             tCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGAt                                   >  2:3730119/1‑148 (MQ=255)
                                                                                                              cTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATa                                  >  2:156961/1‑148 (MQ=255)
                                                                                                              cTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATa                                  <  2:2177922/148‑1 (MQ=255)
                                                                                                                gATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAAc                                <  1:1882838/148‑1 (MQ=255)
                                                                                                                   tGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATc                             >  2:1479246/1‑148 (MQ=255)
                                                                                                                   tGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATc                             >  1:3506036/1‑148 (MQ=255)
                                                                                                                   tGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATc                             <  2:2986592/148‑1 (MQ=255)
                                                                                                                     ccAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATccg                           >  2:2562041/1‑148 (MQ=255)
                                                                                                                        aaaGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATccgccg                        >  2:2027007/1‑148 (MQ=255)
                                                                                                                         aaGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCgg                       >  1:469521/1‑148 (MQ=255)
                                                                                                                         aaGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCgg                       >  1:2003727/1‑148 (MQ=255)
                                                                                                                         aaGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTACTTCACAACCGTTCAGTTTGAAGTTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCAGTCGATAACATCCGCCgg                       >  2:3511659/1‑148 (MQ=255)
                                                                                                                          aGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGa                      >  1:1011360/1‑148 (MQ=255)
                                                                                                                             ttCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGa                   <  1:3098436/148‑1 (MQ=255)
                                                                                                                              tCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGAt                  >  1:3293187/1‑148 (MQ=255)
                                                                                                                              tCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGAt                  <  1:1096287/148‑1 (MQ=255)
                                                                                                                               cACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATc                 >  2:3784256/1‑148 (MQ=255)
                                                                                                                                aCGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATcg                <  2:942638/148‑1 (MQ=255)
                                                                                                                                  gAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATcgcc              >  2:568286/1‑148 (MQ=255)
                                                                                                                                  gAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATcgcc              >  2:646702/1‑148 (MQ=255)
                                                                                                                                   aaTTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATcgccg             >  2:3679810/1‑148 (MQ=255)
                                                                                                                                      ttGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATCGCCGcca          >  1:3221906/1‑148 (MQ=255)
                                                                                                                                            cACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATCGCCGccaacccaa    <  1:2506421/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCCAGCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATCGCCGCCAACCCAACT  >  CP009273/3865718‑3866007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑