Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,892,304 C→A 100% P150P (CCG→CCT)  yieK ← putative 6‑phosphogluconolactonase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,892,3040CA99.0% 659.3 / ‑1.9 202P150P (CCG→CCT) yieKputative 6‑phosphogluconolactonase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (104/96);  total (105/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 202 total aligned reads displayed.

CTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTATCAATA  >  CP009273/3892159‑3892448
                                                                                                                                                 |                                                                                                                                                
cTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAgg                                                                                                                                                >  2:2904096/1‑148 (MQ=255)
 tttACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAggg                                                                                                                                               >  1:441966/1‑148 (MQ=255)
 tttACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAggg                                                                                                                                               >  1:2607492/1‑148 (MQ=255)
 tttACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATAGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCCTCTCTCCCTGGATAggg                                                                                                                                               >  1:1069829/1‑148 (MQ=255)
      ccGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTc                                                                                                                                          >  2:514491/1‑148 (MQ=255)
       cgcgCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTcc                                                                                                                                         >  1:3121635/1‑148 (MQ=255)
       cgcgCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTcc                                                                                                                                         >  2:3044913/1‑148 (MQ=255)
        gcgcCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCa                                                                                                                                        <  2:943758/148‑1 (MQ=255)
        gcgcCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCa                                                                                                                                        <  2:2985956/148‑1 (MQ=255)
         cgccgcTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGACCTCCAc                                                                                                                                       >  1:3509548/1‑148 (MQ=255)
            cgcTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGt                                                                                                                                    <  2:3569247/148‑1 (MQ=255)
             gcTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGtt                                                                                                                                   <  1:1473936/148‑1 (MQ=255)
              cTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGttt                                                                                                                                  >  1:2278741/1‑148 (MQ=255)
                     ttATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATg                                                                                                                           >  2:1948048/1‑148 (MQ=255)
                       aTCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGa                                                                                                                         >  2:3713892/1‑148 (MQ=255)
                        tCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGaa                                                                                                                        >  1:3665495/1‑148 (MQ=255)
                         cAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGaaa                                                                                                                       <  2:1289223/148‑1 (MQ=255)
                               gTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTc                                                                                                                 >  1:2876221/1‑148 (MQ=255)
                                   ttCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTAt                                                                                                             <  1:2635100/148‑1 (MQ=255)
                                     cgccgcCATGATGCTGTTCGGCCCCATCGTCACGGAGCTGTCAGTCACTAACGAAAAGTCGCCGCCCAACGCGCCATGCGCCACAAGATCAACCATCTCTCCCTGGATAGGGAACTCCACTGTTTGCTCATGGAAATGGGTCGTATTc                                                                                                           <  2:3648634/148‑1 (MQ=255)
                                          ccATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCaa                                                                                                      <  2:2379065/148‑1 (MQ=255)
                                          ccATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCaa                                                                                                      <  1:822191/148‑1 (MQ=255)
                                            atgatgCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAAt                                                                                                    <  2:1221541/148‑1 (MQ=255)
                                            atgatgCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAAt                                                                                                    <  1:1759795/148‑1 (MQ=255)
                                            atgatgCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAAt                                                                                                    >  1:2093470/1‑148 (MQ=255)
                                              gatgCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTg                                                                                                  >  1:2177705/1‑148 (MQ=255)
                                              gatgCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTg                                                                                                  >  2:1922651/1‑148 (MQ=255)
                                               atgCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGc                                                                                                 >  1:2244680/1‑148 (MQ=255)
                                                tgCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGcc                                                                                                >  2:1225842/1‑148 (MQ=255)
                                                 gCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCca                                                                                               >  2:914267/1‑148 (MQ=255)
                                                 gCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCca                                                                                               >  1:3555039/1‑148 (MQ=255)
                                                    tttCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACaa                                                                                            <  1:768025/148‑1 (MQ=255)
                                                       cGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACaaaaa                                                                                         <  1:3367341/148‑1 (MQ=255)
                                                        ggCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAAt                                                                                        <  1:2417942/148‑1 (MQ=255)
                                                        ggCACCATCGTCACGTAGCTGTCATGCACTATCTAAAAGTCTCCGCCCAACTCGCCATGCGCCACACTATCAACCATCTCTCCCTGGATAGGGAAATCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAAt                                                                                        <  1:1269310/148‑1 (MQ=255)
                                                         gCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATg                                                                                       >  1:524667/1‑148 (MQ=255)
                                                          ccccATCGTCACGTATCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCTCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGa                                                                                      <  1:3218275/148‑1 (MQ=255)
                                                          ccccATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGa                                                                                      <  1:666278/148‑1 (MQ=255)
                                                          ccccATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGa                                                                                      <  2:2350846/148‑1 (MQ=255)
                                                            ccATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGAcc                                                                                    <  2:595364/148‑1 (MQ=255)
                                                            ccATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGAcc                                                                                    <  2:595306/148‑1 (MQ=255)
                                                              aTCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCAt                                                                                  >  2:1105883/1‑148 (MQ=255)
                                                                  tCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGc                                                                              >  1:3339050/1‑148 (MQ=255)
                                                                   cACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCa                                                                             >  2:1135159/1‑148 (MQ=255)
                                                                     cGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCAcc                                                                           <  2:1510785/148‑1 (MQ=255)
                                                                       tAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTa                                                                         <  2:1379500/148‑1 (MQ=255)
                                                                        aGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTaa                                                                        >  2:2669581/1‑148 (MQ=255)
                                                                         gCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAAt                                                                       <  1:993542/148‑1 (MQ=255)
                                                                          cTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATc                                                                      >  2:2456598/1‑148 (MQ=255)
                                                                          cTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATc                                                                      >  2:3008001/1‑148 (MQ=255)
                                                                                ggCACTAACGAAAAGTCGCCGCCAAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGca                                                                >  2:1818559/1‑148 (MQ=255)
                                                                                    cTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGcaccac                                                            <  1:728581/148‑1 (MQ=255)
                                                                                       aCGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCaa                                                         >  1:2371783/1‑148 (MQ=255)
                                                                                         gAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAAt                                                       <  2:1776633/148‑1 (MQ=255)
                                                                                         gAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAAt                                                       <  2:2607492/148‑1 (MQ=255)
                                                                                         gAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAAt                                                       <  1:179255/148‑1 (MQ=255)
                                                                                           aaaGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATcc                                                     <  2:580200/148‑1 (MQ=255)
                                                                                           aaaGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATcc                                                     >  1:633537/1‑148 (MQ=255)
                                                                                           aaaGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATcc                                                     >  1:1992899/1‑148 (MQ=255)
                                                                                           aaaGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGAACCACCAAAtac                                                     >  2:3742927/1‑146 (MQ=255)
                                                                                           aaaGTCGCCGCCCAACTAGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATcc                                                     >  1:2334400/1‑148 (MQ=255)
                                                                                             aGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAg                                                   >  2:1205620/1‑148 (MQ=255)
                                                                                              gTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGt                                                  >  2:1597700/1‑148 (MQ=255)
                                                                                              gTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGt                                                  >  2:3494990/1‑148 (MQ=255)
                                                                                               tCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTc                                                 <  2:516641/148‑1 (MQ=255)
                                                                                                  ccgccCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGc                                              >  1:2740859/1‑148 (MQ=255)
                                                                                                  ccgccCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGc                                              <  2:3388971/148‑1 (MQ=255)
                                                                                                       cAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCa                                         >  2:2012175/1‑148 (MQ=255)
                                                                                                       cAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCa                                         >  2:3259029/1‑148 (MQ=255)
                                                                                                       cAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCa                                         >  2:2426778/1‑148 (MQ=255)
                                                                                                         aCTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCAcg                                       >  2:3165116/1‑148 (MQ=255)
                                                                                                            cGCCATGCGCCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACgcgc                                    <  1:637227/148‑1 (MQ=255)
                                                                                                                   cgcCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTc                             <  1:2913079/148‑1 (MQ=255)
                                                                                                                   cgcCACAATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTc                             <  2:1908967/148‑1 (MQ=255)
                                                                                                                      cacaATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGa                          <  2:2237166/148‑1 (MQ=255)
                                                                                                                      cacaATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGa                          >  2:786259/1‑148 (MQ=255)
                                                                                                                      cacaATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGa                          <  2:1748497/148‑1 (MQ=255)
                                                                                                                      cacaATATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGa                          <  2:1748434/148‑1 (MQ=255)
                                                                                                                         aaTATCAACCCTCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCa                       <  1:77101/148‑1 (MQ=255)
                                                                                                                         aaTATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCa                       <  1:505638/148‑1 (MQ=255)
                                                                                                                         aaTATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCa                       <  2:2093470/148‑1 (MQ=255)
                                                                                                                         aaTATCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCa                       <  2:3536173/148‑1 (MQ=255)
                                                                                                                           tatCAACCATCTCTCCCTTGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATg                     <  1:2791914/148‑1 (MQ=255)
                                                                                                                           tatCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATg                     >  2:3892/1‑148 (MQ=255)
                                                                                                                           tatCAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATg                     <  2:1034428/148‑1 (MQ=255)
                                                                                                                              cAACCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTc                  >  1:627633/1‑148 (MQ=255)
                                                                                                                                aCCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTcgc                >  2:1431560/1‑148 (MQ=255)
                                                                                                                                aCCATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTcgc                >  2:2558571/1‑148 (MQ=255)
                                                                                                                                 ccATCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTcgcg               >  1:3252883/1‑148 (MQ=255)
                                                                                                                                 ccATCTCTCCCTGGACAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTcgcg               >  1:2449226/1‑148 (MQ=255)
                                                                                                                                   aTCTCTCCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGt             <  2:283810/148‑1 (MQ=255)
                                                                                                                                      tctcCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGt          >  2:455533/1‑148 (MQ=255)
                                                                                                                                       ctcCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGtt         <  2:359147/148‑1 (MQ=255)
                                                                                                                                        tcCCTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTa        >  1:1588766/1‑148 (MQ=255)
                                                                                                                                         cccTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTAt       <  1:963635/148‑1 (MQ=255)
                                                                                                                                         cccTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTAt       <  1:914267/148‑1 (MQ=255)
                                                                                                                                         cccTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTAt       >  1:2884068/1‑148 (MQ=255)
                                                                                                                                         cccTGGATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTAt       >  1:3564400/1‑148 (MQ=255)
                                                                                                                                           cgggATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTATCa     <  2:3060748/146‑1 (MQ=255)
                                                                                                                                             ggATAGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTATCAAt   >  1:1399991/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTTTACCCGCGCCGCTGACAATTATCAGCAAGTTTTTCGCCGCCATGATGCTTTTCGGCCCCATCGTCACGTAGCTGTCAGGCACTAACGAAAAGTCGCCGCCCAACTCGCCATGCGCCACAATATCAACCATCTCTCCCTGGATCGGGAACTCCACGGTTTGCTCATGGAAATGGGTCGTATTCGGCAAATTGCCACAAAAATGACCATCTGCACCTAATCCCAGCACCACCAAATCCAGTCCGCCTTCACGCGCCAGTTTCTGATCATGCTCGCGGTAGTTATCAATA  >  CP009273/3892159‑3892448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑