Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,904,553 T→A 100% P111P (CCA→CCT)  pstS ← periplasmic phosphate binding protein, high‑affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,904,5530TA98.3% 581.9 / 0.0 178P111P (CCA→CCT) pstSperiplasmic phosphate binding protein, high‑affinity
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base A (93/82);  total (95/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 178 total aligned reads displayed.

GGCGTACTACAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCTGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGCC  >  CP009273/3904408‑3904690
                                                                                                                                                 |                                                                                                                                         
ggcgTACTACAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAgg                                                                                                                                         <  1:1366335/148‑1 (MQ=255)
 gcgTACTACAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGa                                                                                                                                        <  1:597755/148‑1 (MQ=255)
 gcgTACTACAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGa                                                                                                                                        <  1:2610932/148‑1 (MQ=255)
  cgTACTACAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGaa                                                                                                                                       <  2:2488035/148‑1 (MQ=255)
  cgTACTACAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGaa                                                                                                                                       <  1:3450071/148‑1 (MQ=255)
  cgTACTACAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGaa                                                                                                                                       >  1:2553940/1‑148 (MQ=255)
     actacAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGt                                                                                                                                    >  2:2708789/1‑148 (MQ=255)
       tacAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTa                                                                                                                                  >  2:508386/1‑148 (MQ=255)
            cAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGc                                                                                                                             >  2:1465210/1‑148 (MQ=255)
              aTGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCa                                                                                                                           >  1:207408/1‑148 (MQ=255)
              aTGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCa                                                                                                                           >  1:207310/1‑148 (MQ=255)
               tGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAg                                                                                                                          >  1:2853550/1‑148 (MQ=255)
                 ttttGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGca                                                                                                                        >  2:3077169/1‑148 (MQ=255)
                  tttGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGcac                                                                                                                       <  2:373955/148‑1 (MQ=255)
                       gAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCAcgc                                                                                                                  >  2:1688276/1‑148 (MQ=255)
                          ggCAGTTTCAGGCCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCAcgccgc                                                                                                               >  1:1048449/1‑148 (MQ=255)
                          ggCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCAcgccgc                                                                                                               >  1:1048257/1‑148 (MQ=255)
                           gCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACgccgcc                                                                                                              >  1:1596291/1‑148 (MQ=255)
                            cAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCa                                                                                                             <  2:2257184/148‑1 (MQ=255)
                                ttCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCa                                                                                                         <  2:1697982/148‑1 (MQ=255)
                                  cAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCTGGAATGTTAACCGCCAGCACCACGCCGCCAATCACg                                                                                                       <  2:230299/148‑1 (MQ=255)
                                   aGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACgg                                                                                                      <  2:2384951/148‑1 (MQ=255)
                                    gACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGt                                                                                                     <  2:1394686/148‑1 (MQ=255)
                                       ccGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCgg                                                                                                  >  2:2020020/1‑148 (MQ=255)
                                          gATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGaa                                                                                               <  1:1088340/148‑1 (MQ=255)
                                           aTTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAAc                                                                                              >  2:1726944/1‑148 (MQ=255)
                                           aTTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAAc                                                                                              >  2:190104/1‑148 (MQ=255)
                                              cAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCTGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTgg                                                                                           <  2:1527859/148‑1 (MQ=255)
                                               aGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGa                                                                                          <  1:2209499/148‑1 (MQ=255)
                                               aGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGa                                                                                          <  1:2013683/148‑1 (MQ=255)
                                                gTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGaa                                                                                         <  1:3009385/148‑1 (MQ=255)
                                                 tttGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAAc                                                                                        >  1:3508069/1‑148 (MQ=255)
                                                      cGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGAcc                                                                                   <  2:2853552/148‑1 (MQ=255)
                                                        aTGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCtt                                                                                 >  1:3271650/1‑148 (MQ=255)
                                                        aTGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCtt                                                                                 >  1:3272245/1‑148 (MQ=255)
                                                        aTGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCtt                                                                                 <  2:1040681/148‑1 (MQ=255)
                                                           gCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCt                                                                              >  2:1783643/1‑148 (MQ=255)
                                                              tcatcaTCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAg                                                                           <  1:3517657/148‑1 (MQ=255)
                                                               catcatCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGc                                                                          >  1:166251/1‑148 (MQ=255)
                                                                 tcatcCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCa                                                                        >  1:2904625/1‑148 (MQ=255)
                                                                  catcCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAg                                                                       >  1:2672424/1‑148 (MQ=255)
                                                                  catcCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAg                                                                       <  2:207310/148‑1 (MQ=255)
                                                                  catcCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAg                                                                       <  2:207408/148‑1 (MQ=255)
                                                                  catcCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAg                                                                       >  2:3117291/1‑148 (MQ=255)
                                                                     cccACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGttt                                                                    >  1:3164814/1‑148 (MQ=255)
                                                                      ccACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGtttt                                                                   <  1:909688/148‑1 (MQ=255)
                                                                      ccACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGtttt                                                                   <  1:1154694/148‑1 (MQ=255)
                                                                       cACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGttttt                                                                  >  1:3812179/1‑148 (MQ=255)
                                                                       cACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGttttt                                                                  >  2:2914281/1‑148 (MQ=255)
                                                                       cACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGttttt                                                                  >  1:3811874/1‑148 (MQ=255)
                                                                              tGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGa                                                           <  1:2303803/148‑1 (MQ=255)
                                                                                 ttttGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAg                                                        <  1:1364806/148‑1 (MQ=255)
                                                                                 ttttGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAg                                                        <  1:1364819/148‑1 (MQ=255)
                                                                                  tttGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGACTGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAgc                                                       >  2:2753701/1‑148 (MQ=255)
                                                                                  tttGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAgc                                                       >  2:2758345/1‑148 (MQ=255)
                                                                                  tttGCCAAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAgc                                                       >  2:3192454/1‑148 (MQ=255)
                                                                                      cccAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAgcggcg                                                   <  2:280280/148‑1 (MQ=255)
                                                                                      cccAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAgcggcg                                                   >  1:3771321/1‑148 (MQ=255)
                                                                                      cccAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCACAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAgcggcg                                                   >  2:3083151/1‑148 (MQ=255)
                                                                                         aGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGt                                                >  2:3611296/1‑148 (MQ=255)
                                                                                          ggTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTc                                               >  2:3490025/1‑148 (MQ=255)
                                                                                           gTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCa                                              >  2:448732/1‑148 (MQ=255)
                                                                                           gTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCa                                              >  1:2457562/1‑148 (MQ=255)
                                                                                           gTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCa                                              >  2:508305/1‑148 (MQ=255)
                                                                                           gTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCa                                              <  2:3364281/148‑1 (MQ=255)
                                                                                            tAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCag                                             >  1:1624732/1‑148 (MQ=255)
                                                                                            tAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCag                                             <  1:199838/148‑1 (MQ=255)
                                                                                            tAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCag                                             >  2:2827598/1‑148 (MQ=255)
                                                                                            tAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCag                                             <  2:239957/148‑1 (MQ=255)
                                                                                              gATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCagag                                           >  2:225470/1‑148 (MQ=255)
                                                                                              gATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCagag                                           >  1:2677382/1‑148 (MQ=255)
                                                                                               aTGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCTGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAgg                                          >  1:3187839/1‑148 (MQ=255)
                                                                                                tGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGc                                         <  1:2868972/148‑1 (MQ=255)
                                                                                                 gTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCa                                        >  2:1093000/1‑148 (MQ=255)
                                                                                                     ccGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCaa                                    >  1:1833371/1‑148 (MQ=255)
                                                                                                        aGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAAt                                 <  1:735704/148‑1 (MQ=255)
                                                                                                           gTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCaa                              >  2:1579182/1‑148 (MQ=255)
                                                                                                            ttttACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAAc                             <  1:1688276/148‑1 (MQ=255)
                                                                                                            ttttACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAAc                             <  2:2519533/148‑1 (MQ=255)
                                                                                                             tttACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACg                            >  2:129343/1‑148 (MQ=255)
                                                                                                             tttACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACg                            <  2:2210713/148‑1 (MQ=255)
                                                                                                              ttACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACgg                           <  1:846883/148‑1 (MQ=255)
                                                                                                               tACCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGt                          <  2:3375528/148‑1 (MQ=255)
                                                                                                                aCCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTa                         <  1:3077169/148‑1 (MQ=255)
                                                                                                                aCCATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTa                         <  2:2887731/148‑1 (MQ=255)
                                                                                                                  cATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTAtt                       <  1:94989/148‑1 (MQ=255)
                                                                                                                  cATCCAGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTAtt                       <  1:323194/148‑1 (MQ=255)
                                                                                                                       aGCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGa                  >  2:9928/1‑148 (MQ=255)
                                                                                                                        gCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGAt                 >  2:2232603/1‑148 (MQ=255)
                                                                                                                        gCACCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGAt                 >  2:1019845/1‑148 (MQ=255)
                                                                                                                          aCCAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATaa               <  1:2715719/148‑1 (MQ=255)
                                                                                                                           ccAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAAt              <  1:453368/148‑1 (MQ=255)
                                                                                                                           ccAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAAt              <  1:1465210/148‑1 (MQ=255)
                                                                                                                            cAGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATc             <  2:718406/148‑1 (MQ=255)
                                                                                                                             aGTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCt            <  1:684669/148‑1 (MQ=255)
                                                                                                                              gTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTg           >  2:3598503/1‑148 (MQ=255)
                                                                                                                              gTTCGCCAGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTg           <  2:723469/148‑1 (MQ=255)
                                                                                                                                     aGACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACg    >  1:2928597/1‑148 (MQ=255)
                                                                                                                                      gACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGc   >  1:705503/1‑148 (MQ=255)
                                                                                                                                      gACTTCAGCCCAGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGc   >  1:705498/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                         
GGCGTACTACAGCAATGTTTTGTGAAGGCAGTTTCAGACCCGGATTCAGTTTGGCGATGGCTTCATCATCCCACTTCTTGATTTTGCCCAGGTAGATGTCGCCGAGGGTTTTACCATCCAGCACCAGTTCGCCAGACTTCAGCCCTGGAATGTTAACCGCCAGCACCACGCCGCCAATCACGGTCGGGAACTGGAACAGACCTTCCTGAGCCAGTTTTTCGTCAGACAGCGGCGCGTCAGAGGCACCAAAATCAACGGTATTAGCGATAATCTGTTTTACGCC  >  CP009273/3904408‑3904690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑