Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,906,384 G→T 100% I215I (ATC→ATA)  glmS ← L‑glutamine:D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,906,3840GT98.1% 649.8 / 1.8 209I215I (ATC→ATA) glmSL‑glutamine:D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base T (119/86);  total (122/87)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 209 total aligned reads displayed.

TCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGG  >  CP009273/3906239‑3906530
                                                                                                                                                 |                                                                                                                                                  
 cgcgTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTAtt                                                                                                                                                 >  1:3206598/1‑148 (MQ=255)
  gcgTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTAttt                                                                                                                                                <  1:1542525/148‑1 (MQ=255)
   cgTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTc                                                                                                                                               <  1:3322364/148‑1 (MQ=255)
           tGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAatat                                                                                                                                       >  2:1105586/1‑148 (MQ=255)
            gTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATc                                                                                                                                      >  1:2966578/1‑148 (MQ=255)
            gTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACATCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATc                                                                                                                                      >  2:435134/1‑148 (MQ=255)
                  aGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCctc                                                                                                                                <  2:3405708/148‑1 (MQ=255)
                  aGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCctc                                                                                                                                >  1:1509536/1‑148 (MQ=255)
                  aGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCctc                                                                                                                                <  1:2188896/148‑1 (MQ=255)
                  aGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCctc                                                                                                                                <  1:2074631/148‑1 (MQ=255)
                   gATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCctct                                                                                                                               >  2:2061750/1‑148 (MQ=255)
                    aTCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCtt                                                                                                                              <  2:953071/148‑1 (MQ=255)
                    aTCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCtt                                                                                                                              <  2:3450543/148‑1 (MQ=255)
                     tctcTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTc                                                                                                                             >  1:1039721/1‑148 (MQ=255)
                     tctcTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTAACGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTc                                                                                                                             >  1:2732232/1‑148 (MQ=255)
                     tctcTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGCCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTc                                                                                                                             >  1:1194164/1‑148 (MQ=255)
                      ctctTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCa                                                                                                                            <  1:3547475/148‑1 (MQ=255)
                       tctTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCaa                                                                                                                           <  1:866966/148‑1 (MQ=255)
                          ttCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGa                                                                                                                        >  1:3033735/1‑148 (MQ=255)
                            cTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAg                                                                                                                      <  2:672013/148‑1 (MQ=255)
                            cTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAg                                                                                                                      <  1:2498332/148‑1 (MQ=255)
                                aTGTAGTGACGGTAAATGCCTTTATCGCCCTCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTTTCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATaa                                                                                                                  <  1:2617137/148‑1 (MQ=255)
                                aTGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATaa                                                                                                                  <  1:684914/148‑1 (MQ=255)
                                aTGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATaa                                                                                                                  <  1:684780/148‑1 (MQ=255)
                                 tGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATaaa                                                                                                                 >  2:3477277/1‑148 (MQ=255)
                                 tGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATaaa                                                                                                                 <  1:1639516/148‑1 (MQ=255)
                                      tGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGAc                                                                                                            >  1:1981059/1‑148 (MQ=255)
                                      tGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGAc                                                                                                            >  1:3707522/1‑148 (MQ=255)
                                      tGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGAc                                                                                                            >  1:3128047/1‑148 (MQ=255)
                                      tGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAAAATCGCCCTCTTAAAGGAAGATAAAGCGAc                                                                                                            >  1:1476565/1‑148 (MQ=255)
                                              aaTGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCAcc                                                                                                    >  2:2786019/1‑148 (MQ=255)
                                                 gCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGc                                                                                                 >  2:1070427/1‑148 (MQ=255)
                                                 gCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATAGCCCTCTTCAATGAAGATAAAGCGACGGGTCACCGGc                                                                                                 >  1:3217488/1‑148 (MQ=255)
                                                     ttATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACa                                                                                             >  2:1266150/1‑148 (MQ=255)
                                                        tCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAgcg                                                                                          >  1:1810435/1‑148 (MQ=255)
                                                          gCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGcc                                                                                        >  1:2283052/1‑148 (MQ=255)
                                                            ccGCTTCATTTTGCTTTTTGGTTTCGATAGCCTGACGTTTTACTCCCGCGCCAGTTTTAGCGAATATTTTTCCCGACCGGCGAGTTATTTCCGAAATACCGCCCTCTAAACGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAg                                                                                      <  2:3562401/148‑1 (MQ=255)
                                                                 tCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCACCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGt                                                                                 >  1:1777912/1‑148 (MQ=255)
                                                                  cATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTc                                                                                >  1:2598658/1‑148 (MQ=255)
                                                                  cATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTc                                                                                >  1:2598674/1‑148 (MQ=255)
                                                                  cATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTc                                                                                >  1:737023/1‑148 (MQ=255)
                                                                  cATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTc                                                                                >  2:2418311/1‑148 (MQ=255)
                                                                     atTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGa                                                                             >  1:3077141/1‑148 (MQ=255)
                                                                          tgtttGGTTTCGATATCGTGACGTTTTACTTCCGCGCCTGTTTTATCGAAGTTGTTTACCGAACGGCGAGTTTTTGCCGAAATATCGCCCTCTTCAAGTAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGa                                                                        <  2:1413074/145‑1 (MQ=255)
                                                                             ttGTATTCGATATCCTGACGTTTTACTTCCGCGCCATTTTTAACGAATTTTTTTACCGAACGGCTATTTATTTACGCAATATCGCACTCTTAATGGAAGAAAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATaa                                                                     <  2:2808856/148‑1 (MQ=255)
                                                                             ttGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATaa                                                                     >  2:2522865/1‑148 (MQ=255)
                                                                              tGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATaaa                                                                    >  2:3306554/1‑148 (MQ=255)
                                                                              tGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATaaa                                                                    >  2:1373641/1‑148 (MQ=255)
                                                                                 tttCGATATCCTGACGTTTAACTTCCGCGCCAGTTTTATCTAATATGTTTACCGAACGGCGTGTTATTTCCGCAATATCGCCCTCTACAAGGAATATAACGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGtt                                                                 <  2:2610122/147‑1 (MQ=255)
                                                                                 aTTCGATATCCTGACGTTTTCCTTCCGCGCCTGTTTTATCTAAGTTTTTTACCGACCGGCGAGTTTTTTCCGCAATATCCCCCTCTTCACGTAACATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGtt                                                                 <  2:693197/148‑1 (MQ=255)
                                                                                 aTTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGtt                                                                 >  2:1467391/1‑148 (MQ=255)
                                                                                 aTTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGtt                                                                 >  1:469427/1‑148 (MQ=255)
                                                                                  ttCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGTTGTTTCCCGAACGGCTAGTTATTTCCGCAATATCGCCCTCTTCAAGTAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGttt                                                                <  2:1833474/148‑1 (MQ=255)
                                                                                  ttCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGttt                                                                >  2:1143545/1‑148 (MQ=255)
                                                                                  ttCGATATCCGGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGTGTTATTTCCGCAATATCGCCCTCTTCATGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGttt                                                                <  2:205425/148‑1 (MQ=255)
                                                                                   tctatatCCTGACGTTTTACTTCCGCGCCCGTTTTATCGAAGATGTTAACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGACTATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGtttt                                                               <  2:1881005/145‑1 (MQ=255)
                                                                                   tCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGtttt                                                               >  1:3414667/1‑148 (MQ=255)
                                                                                   tCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGtttt                                                               >  1:3412827/1‑148 (MQ=255)
                                                                                   tCGATATCCTGACGTTTTACTTCCGCGCAAGTTTTAGCTAATTTGTTTACCGAACGCGGAGTTTTTGCCGCACTTTCGCCCTCTGCATGGAAGTTAACGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGtttt                                                               <  2:1736167/148‑1 (MQ=255)
                                                                                      atatCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGc                                                            >  1:1243277/1‑148 (MQ=255)
                                                                                             cagtttttACTCCGGCGCCTGTTTTATCGATTTTTTTTACGGAACGGCTATTTTTTTCCGAAATAGCGCCCTCTTCATGGATGATAACGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATccc                                                     <  2:664708/144‑1 (MQ=255)
                                                                                              aCGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCGGCTGGTCAGAAGCGATAAAGTTTTCGCCCATcccc                                                    >  1:3453343/1‑148 (MQ=255)
                                                                                              aCGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATcccc                                                    >  2:601654/1‑148 (MQ=255)
                                                                                              aCGTTTTACTACCGCGCCCTTTTTATCGAATATGTTTCCCGAACGGCGTTTTTTTTCCGCAATAGCGCCCTCTTCATGTAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATcccc                                                    <  2:2341561/148‑1 (MQ=255)
                                                                                               cGTTTTAGTTCCGCGCCAGTTTTATCGAAGTTGTTTACCTATGGGCGAGTTTTTTCCGCAATAGCGCCCTCTTCATGGATTATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCa                                                   <  2:1841351/148‑1 (MQ=255)
                                                                                                  tttACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGc                                                >  1:2102870/1‑148 (MQ=255)
                                                                                                      cTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAAt                                            >  1:1549304/1‑148 (MQ=255)
                                                                                                      cTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAAt                                            >  1:2743984/1‑148 (MQ=255)
                                                                                                      cTTCCGCGCAATTTTTATCTAAGATGTTTACCGAACGGCGATTTATTTCCGCAATATCGCCCTCTACAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAAt                                            <  2:2949202/148‑1 (MQ=255)
                                                                                                       ttCCGCGCCTTTTTTATGTAATATTTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCATCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATc                                           <  2:2764651/148‑1 (MQ=255)
                                                                                                       ttCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATc                                           >  2:163747/1‑148 (MQ=255)
                                                                                                       ttCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATc                                           >  2:3789008/1‑148 (MQ=255)
                                                                                                        tCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCa                                          >  1:338482/1‑148 (MQ=255)
                                                                                                        tCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCa                                          >  1:2158299/1‑148 (MQ=255)
                                                                                                         ccGCGCCTTTTTTATCGAATTTGTTGACCGCACGGCTTTTTTTTCCCGAAATAGCGCCCTCTAAAAGAAATATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCAc                                         <  1:1115990/148‑1 (MQ=255)
                                                                                                                ttttttATCTAAGATGTTTACCTAACGGCGAGTTTTTTCCGCAATATCGCCCTCTTCAAGGAATATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGa                                  <  2:3407984/146‑1 (MQ=255)
                                                                                                                tgTTTTATCTATTATGTTTACCGAACGGCGATTTATTTCCGAAATATCGCCCTCTTCATGGAAGATAACGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGa                                  <  2:1509474/147‑1 (MQ=255)
                                                                                                                aGTTTTATCTAAGTTGTTTACCGAACGGCGTGTTATTTCCGAAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGa                                  <  2:1509637/148‑1 (MQ=255)
                                                                                                                  ttttATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATc                                                       >  1:2201955/1‑125 (MQ=255)
                                                                                                                  ttttATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACt                                >  2:2652417/1‑148 (MQ=255)
                                                                                                                    ttATCGAAGATGTTTCCCGAACGGCGAGTTATTTCCGAAATATCGCCCTCTACAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTAc                              <  2:876479/148‑1 (MQ=255)
                                                                                                                      aTCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCa                            >  2:405990/1‑148 (MQ=255)
                                                                                                                        ctaaGTTGTTAACCGACCGGCGAGTTATTTCCGCAATAGCGCCCTCTTCAAGGAAGATAACGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGa                          <  2:1159768/146‑1 (MQ=255)
                                                                                                                        cGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGa                          >  1:1665444/1‑148 (MQ=255)
                                                                                                                             atttttACCGAACGGCGTGTTATTTCCGAAATATCGCCCTATTCATGCAATATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTg                     <  2:3206598/145‑1 (MQ=255)
                                                                                                                             aTGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTg                     >  2:313610/1‑148 (MQ=255)
                                                                                                                             aTGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTg                     >  2:2131861/1‑148 (MQ=255)
                                                                                                                                   aCCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCca               <  2:3707522/148‑1 (MQ=255)
                                                                                                                                   aCCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCca               >  1:3534586/1‑148 (MQ=255)
                                                                                                                                     cGAAGGGCGAGTTATGCCCGCAATATCGCCCTAAAAAAGCACCAAAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCcagc             <  1:2417279/148‑1 (MQ=255)
                                                                                                                                     cGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCACGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCcagc             <  2:2215297/148‑1 (MQ=255)
                                                                                                                                        aCGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAgg          >  1:2252153/1‑148 (MQ=255)
                                                                                                                                            cGAGTTATTTCCGCAATATCGCCCTCTTCAAGGATGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTAt      <  2:3628009/148‑1 (MQ=255)
                                                                                                                                            cGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTAt      >  2:3389210/1‑148 (MQ=255)
                                                                                                                                            cGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTAt      >  2:2796801/1‑148 (MQ=255)
                                                                                                                                            cGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTAt      >  1:1290712/1‑148 (MQ=255)
                                                                                                                                              aGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATcc    >  2:3156843/1‑148 (MQ=255)
                                                                                                                                              aGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATcc    >  1:2229432/1‑148 (MQ=255)
                                                                                                                                              aGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATcc    >  1:1968519/1‑148 (MQ=255)
                                                                                                                                                tGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCgg  >  2:347063/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCCGG  >  CP009273/3906239‑3906530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑