Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,910,448 G→T 100% V96V (GTC→GTA)  atpD ← F1 sector of membrane‑bound ATP synthase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,910,4480GT98.9% 578.5 / ‑2.9 179V96V (GTC→GTA) atpDF1 sector of membrane‑bound ATP synthase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (88/89);  total (89/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 179 total aligned reads displayed.

CCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCG  >  CP009273/3910305‑3910592
                                                                                                                                               |                                                                                                                                                
ccGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCg                                                                                                                                              >  2:1523249/1‑148 (MQ=255)
 cGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCgg                                                                                                                                             >  2:2389554/1‑148 (MQ=255)
   cccTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGtt                                                                                                                                           <  2:1052427/148‑1 (MQ=255)
   cccTGAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGtt                                                                                                                                           <  2:1052478/148‑1 (MQ=255)
      ttAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCAc                                                                                                                                        >  2:2256116/1‑148 (MQ=255)
          cGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAg                                                                                                                                    <  1:1380240/148‑1 (MQ=255)
          cGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAg                                                                                                                                    >  2:478877/1‑148 (MQ=255)
           gAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGt                                                                                                                                   <  2:736612/148‑1 (MQ=255)
              cGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACg                                                                                                                                >  2:2958849/1‑148 (MQ=255)
              cGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACg                                                                                                                                >  2:2958851/1‑148 (MQ=255)
               gtacacATCATGTCTATAACTTTGATACCTTTTTCCAGCAGTTCCTGAGAGTTTGTCAGCTCTTCGTATGAAGGTGCTGAGAGGTGTATAGCCCAAAGCTCTTCTTCAACGATCTCTCCTTTCATGTCTACAGGTTCACCCAGTACGt                                                                                                                               <  1:2397233/146‑1 (MQ=255)
               ggACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGt                                                                                                                               <  1:1076848/148‑1 (MQ=255)
                gACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGtt                                                                                                                              <  2:1266112/148‑1 (MQ=255)
                 acacATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTc                                                                                                                             <  2:3077569/148‑1 (MQ=255)
                  cacaTCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCa                                                                                                                            >  2:3257733/1‑148 (MQ=255)
                   acaTCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCAt                                                                                                                           <  1:1193659/148‑1 (MQ=255)
                      tCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGAt                                                                                                                        <  1:1207123/148‑1 (MQ=255)
                       cAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATa                                                                                                                       >  2:1822383/1‑148 (MQ=255)
                       cAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATa                                                                                                                       >  1:133245/1‑148 (MQ=255)
                          gTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACgg                                                                                                                    >  2:2893769/1‑148 (MQ=255)
                          gTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACgg                                                                                                                    >  1:1795330/1‑148 (MQ=255)
                            cGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGcc                                                                                                                  <  2:949605/148‑1 (MQ=255)
                                  cTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGt                                                                                                            <  1:478877/148‑1 (MQ=255)
                                  cTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGt                                                                                                            >  2:335665/1‑148 (MQ=255)
                                  cTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGt                                                                                                            >  1:3077310/1‑148 (MQ=255)
                                  cTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGt                                                                                                            >  2:2721640/1‑148 (MQ=255)
                                   tttGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTc                                                                                                           >  1:3008963/1‑148 (MQ=255)
                                     tGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGc                                                                                                         <  1:1589743/148‑1 (MQ=255)
                                     tGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGc                                                                                                         <  2:1708182/148‑1 (MQ=255)
                                     tGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGc                                                                                                         <  2:2358827/148‑1 (MQ=255)
                                     tGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGc                                                                                                         <  1:2616516/148‑1 (MQ=255)
                                     tGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGc                                                                                                         <  2:436192/148‑1 (MQ=255)
                                         aCCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTta                                                                                                     >  2:1846461/1‑148 (MQ=255)
                                         aCCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTta                                                                                                     >  2:3763748/1‑148 (MQ=255)
                                         aCCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTta                                                                                                     >  2:3763753/1‑148 (MQ=255)
                                         aCCGGTTTCCAGCAGTTCCAGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAAACGCCCAAACCTCTTCTTCACCGATCTCGCCTTTCATGTCTAACGGTTCACCCAGTCCGTTCATGATACTGCCCAGAGTCGcgata                                                                                                     >  2:2862651/1‑144 (MQ=255)
                                           cGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTtacc                                                                                                   <  2:2765751/148‑1 (MQ=255)
                                            ggTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTtacct                                                                                                  <  2:2274740/148‑1 (MQ=255)
                                              tttCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTtacctac                                                                                                <  1:3488926/148‑1 (MQ=255)
                                                   agcagTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGa                                                                                           <  1:2446902/148‑1 (MQ=255)
                                                    gcagTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTTACTACCCGGAc                                                                                          >  2:2350090/1‑148 (MQ=255)
                                                    gcagTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGAc                                                                                          <  2:2476163/148‑1 (MQ=255)
                                                        ttCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCa                                                                                      <  1:2643568/148‑1 (MQ=255)
                                                        ttCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCa                                                                                      <  2:1809983/148‑1 (MQ=255)
                                                        ttCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCa                                                                                      <  1:1043271/148‑1 (MQ=255)
                                                        ttCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTACTAACGGGGCGTCa                                                                                      >  1:1015080/1‑148 (MQ=255)
                                                         tCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCaa                                                                                     <  1:1010328/148‑1 (MQ=255)
                                                         tCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCaa                                                                                     <  1:1845929/148‑1 (MQ=255)
                                                             gagaGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTAACTACCGGGCCGTCAATAgg                                                                                 >  1:1369423/1‑148 (MQ=255)
                                                                 gTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGgtgt                                                                             <  1:2958849/148‑1 (MQ=255)
                                                                  tttGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCAAACCGGCCTTCAATACGGTGtt                                                                            >  2:1786867/1‑148 (MQ=255)
                                                                  tttGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTAACTACCGGGCCTTGAATCGGGTGtt                                                                            >  2:124247/1‑148 (MQ=255)
                                                                   ttGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTAACGGGACATAAATCGGGTGTTc                                                                           >  2:2143803/1‑148 (MQ=255)
                                                                      aCAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTCCGACCGGGCCTTCAATCGGGTGGTGGAg                                                                        >  2:1422640/1‑148 (MQ=255)
                                                                         gCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTTCAAAACGGGCCTTCAAACGGGGGTTGGCGGtg                                                                     >  1:2636972/1‑147 (MQ=255)
                                                                         gCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTc                                                                     <  2:2492789/148‑1 (MQ=255)
                                                                         gCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTc                                                                     <  1:2256116/148‑1 (MQ=255)
                                                                         gCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTc                                                                     <  1:2256047/148‑1 (MQ=255)
                                                                         gCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTAACTACCGGGACTTCAATCGGGTGTTCGAGGtg                                                                     >  2:866621/1‑147 (MQ=255)
                                                                            ctTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCttt                                                                  <  1:3714033/148‑1 (MQ=255)
                                                                            ctTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCttt                                                                  <  2:1969984/148‑1 (MQ=255)
                                                                             ttCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTACCTACCGGGCCGTCAAACGGGTGGTCGAGGTCtttt                                                                 >  2:1671923/1‑148 (MQ=255)
                                                                              tCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTa                                                                <  1:2672211/148‑1 (MQ=255)
                                                                               cGTAGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTAc                                                               <  1:711137/148‑1 (MQ=255)
                                                                                  aGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGCCTTCAATCGGGGGTTCGAGGTCTTTTACATc                                                            >  2:3332712/1‑148 (MQ=255)
                                                                                  aGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATc                                                            <  1:685793/148‑1 (MQ=255)
                                                                                  aGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATc                                                            <  2:3199029/148‑1 (MQ=255)
                                                                                  aGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATc                                                            <  1:1919199/148‑1 (MQ=255)
                                                                                  aGGAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACGTCAATCGGGTGTTCGAGGTCTTTTACATc                                                            >  2:3331837/1‑148 (MQ=255)
                                                                                   ggAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATcc                                                           <  2:504650/148‑1 (MQ=255)
                                                                                   ggAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTCCTACCCGGGCGGCAATCGGGTGTTCGAGGTCTTTTACAAcc                                                           >  2:504750/1‑148 (MQ=255)
                                                                                    gAAGGTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCCGGTGTTAGAGGTCTTTTAAATCCa                                                          >  2:2080642/1‑148 (MQ=255)
                                                                                       ggTGCTGCGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGAc                                                       <  2:1795330/148‑1 (MQ=255)
                                                                                           ctgcGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTCCTACCCGGCCTTCAATCGGGTGTTTGAGGTGTTTTTGATACAGACcccg                                                   >  1:1480628/1‑148 (MQ=255)
                                                                                           ctgcGCGGTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTACAGACCCGGGCTTGAAACGGGGGTTGGGGGTGGTTTAAATACacaacccg                                                   >  2:1315023/1‑141 (MQ=255)
                                                                                                  gTGAATCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGa                                            <  1:652676/148‑1 (MQ=255)
                                                                                                      aTCGCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTTCCAACCGGGCCGTCAATAGGGGGTTAGAGGTCTTTTAAATACAGACCCCGACCCACAccca                                        >  1:3459943/1‑146 (MQ=255)
                                                                                                         gCCCAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGGTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCgg                                     <  2:1129697/148‑1 (MQ=255)
                                                                                                           ccAAAGCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCggag                                   <  2:501452/148‑1 (MQ=255)
                                                                                                             aaagCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCggagga                                 <  2:1606783/145‑1 (MQ=255)
                                                                                                               agCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCCGGGCGTAAATAGGGTGTTAGAGGTGTTTTTAATACACACCCCCACGCACACCCTCGGAGGAAc                               >  1:1524316/2‑148 (MQ=255)
                                                                                                                gCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAAcc                              <  1:3416676/148‑1 (MQ=255)
                                                                                                                gCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTACAACCCGGTCGTCAATAGGGTGTTCGAGGTCTTTTTGATCCAGACAGCGACGCACACCCTCGGAGGaaac                              >  1:2814379/1‑146 (MQ=255)
                                                                                                                gCTCTTCTTCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTAACAACCGGGGCGTCAAAAGGGTGTTAGTGGTCTTTTTCATCCAGACCGCGACCCAGCCCGGCGGCGGGAcc                              >  1:2283195/1‑148 (MQ=255)
                                                                                                                   cttcttCACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCAt                           <  1:2350090/148‑1 (MQ=255)
                                                                                                                     tcttcACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTACAACCCGGCCCTCAAAACGGTGTTGGAGGTGTTTTTAAAACAGACCCCGACCCCGACCCGCGGAGGAcccccaag                         >  1:1465657/1‑144 (MQ=255)
                                                                                                                      cttcACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTAACAAACGGGACTTCAAAAGGGGGTTGGTGGTCTTTTACATCCAGACCGCGACGCAGACCGGCGGAGGAACCCAATga                        >  2:1197647/1‑147 (MQ=255)
                                                                                                                      cttcACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCGAACCGGCGTTCAATCGGGTGTTCGAGGTCTGTTACATCCCGACCGCGACGCAGACCGGCGGCGGACCCCATTGc                        >  2:3573243/1‑148 (MQ=255)
                                                                                                                        tcACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGa                      <  1:1742895/148‑1 (MQ=255)
                                                                                                                        tcACCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTTGCGACCGGGGCGTCAATCGGGTGTTGGAGGTGTTTTTAATCCAGACCGCGACGCCGACCCTCGGAGGGACCCCATGCGa                      >  1:2108546/1‑148 (MQ=255)
                                                                                                                          aCCGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTACCAACCCGGCCGTCAATCGGGGGTTCGCGGGCTTTTAACTACACACCGCCACGCACACCGTCGGAGGAACCCCATGCGaag                    >  2:1743332/1‑146 (MQ=255)
                                                                                                                           ccGATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTTGCAACCCGGCCTTCAATACGGTGTTCGGGGTGTTTTTGAAACACACCGCGACGCACACCCTCGGAGGAACCCAATGCGATgg                   >  1:2661874/1‑148 (MQ=255)
                                                                                                                             gATCTCGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGgta                 <  1:535139/148‑1 (MQ=255)
                                                                                                                               tctcGCCTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGgtacg               <  1:2893769/148‑1 (MQ=255)
                                                                                                                                    ccTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCAACCGGGACTTCAAAAGGGGGTTAGAGGTCTTTTAAAAACACACCCCGCCGCAGACCGGCGGAGGAACCCCATGCGGTGGttcgtacgc          >  2:3246005/1‑147 (MQ=255)
                                                                                                                                     cTTTCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGAt         <  1:3137311/148‑1 (MQ=255)
                                                                                                                                      tttCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATa        <  1:836620/148‑1 (MQ=255)
                                                                                                                                       ttCATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATAc       <  1:335665/148‑1 (MQ=255)
                                                                                                                                         cATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATAccg     <  1:178196/148‑1 (MQ=255)
                                                                                                                                         cATGTCTACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCGTTACCTAACCGGACTTCAATCGGGTGTTCGAGGTCTTTTAAATACAGACCCCCACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTAAGATAccg     >  2:1906733/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                
CCGCCCTTAGCGAACGGACACATCAGGTCGATAACTTTGATACCGGTTTCCAGCAGTTCCTGAGAGTTTGACAGCTCTTCGTAGGAAGGTGCTGCGCGGTGAATCGCCCAACGCTCTTCTTCACCGATCTCGCCTTTCATGTCGACCGGTTCACCCAGTACGTTCATGATACGGCCCAGAGTCGCTTTACCTACCGGGACTTCAATCGGGTGTTCGAGGTCTTTTACATCCAGACCGCGACGCAGACCGTCGGAGGAACCCATTGCGATGGTACGTACGATACCGCCG  >  CP009273/3910305‑3910592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑