Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 32,659 C→A 100% R615S (CGC→AGC)  carB → carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927332,6590CA98.8% 520.1 / ‑3.1 160R615S (CGC→AGC) carBcarbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (86/72);  total (88/72)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 160 total aligned reads displayed.

CGGCGGCCCGAACCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATC  >  CP009273/32514‑32802
                                                                                                                                                 |                                                                                                                                               
cggcggcCCGAACCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGc                                                                                                                                               <  2:2880677/148‑1 (MQ=255)
  gcggcCCGAACCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCct                                                                                                                                             <  2:1795786/148‑1 (MQ=255)
        cGAACCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACtt                                                                                                                                       >  2:722802/1‑148 (MQ=255)
           aCCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGa                                                                                                                                    >  1:3491942/1‑148 (MQ=255)
               tATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCg                                                                                                                                >  2:782974/1‑148 (MQ=255)
                aTCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCgg                                                                                                                               >  2:2403373/1‑148 (MQ=255)
                aTCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCgg                                                                                                                               >  2:2976328/1‑148 (MQ=255)
                 tCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGt                                                                                                                              <  2:873886/148‑1 (MQ=255)
                    gTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAAc                                                                                                                           <  1:2197619/148‑1 (MQ=255)
                     tCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAAct                                                                                                                          <  1:3557557/148‑1 (MQ=255)
                        gggTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTg                                                                                                                       <  2:1177814/148‑1 (MQ=255)
                           tATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGaa                                                                                                                    >  2:1636061/1‑148 (MQ=255)
                            aTCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAg                                                                                                                   >  1:3576625/1‑148 (MQ=255)
                              cGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGAt                                                                                                                 <  1:2549538/148‑1 (MQ=255)
                               gAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGAtg                                                                                                                <  2:903942/148‑1 (MQ=255)
                                aaTTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGAtgt                                                                                                               >  1:1871771/1‑148 (MQ=255)
                                aaTTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGAtgt                                                                                                               >  1:3761597/1‑148 (MQ=255)
                                 aTTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGAtgtg                                                                                                              <  1:1740245/148‑1 (MQ=255)
                                 aTTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGAtgtg                                                                                                              <  1:1740311/148‑1 (MQ=255)
                                  ttCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGc                                                                                                             >  2:2978175/1‑148 (MQ=255)
                                     gACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTgg                                                                                                          <  1:3313797/148‑1 (MQ=255)
                                      actactGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGa                                                                                                         >  1:1303519/1‑148 (MQ=255)
                                       ctactGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGaa                                                                                                        <  2:3553163/148‑1 (MQ=255)
                                        tactGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGaaa                                                                                                       <  2:1626046/148‑1 (MQ=255)
                                        tactGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGaaa                                                                                                       >  1:2041775/1‑148 (MQ=255)
                                             ttGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTg                                                                                                  <  1:2988159/148‑1 (MQ=255)
                                             gtGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTg                                                                                                  <  2:3724174/147‑1 (MQ=255)
                                                cGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGt                                                                                               <  2:410596/148‑1 (MQ=255)
                                                cGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGt                                                                                               <  1:638887/148‑1 (MQ=255)
                                                cGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGGAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGt                                                                                               <  2:3163802/148‑1 (MQ=255)
                                                 gTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTa                                                                                              <  1:2842818/148‑1 (MQ=255)
                                                   acacGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATc                                                                                            >  1:1545232/1‑148 (MQ=255)
                                                      cGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCgag                                                                                         >  1:1704380/1‑148 (MQ=255)
                                                           cGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGcc                                                                                    >  2:1428088/1‑148 (MQ=255)
                                                           cGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGcc                                                                                    >  2:1428061/1‑148 (MQ=255)
                                                           cGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGcc                                                                                    >  1:1993858/1‑148 (MQ=255)
                                                            gCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCg                                                                                   >  1:2880293/1‑148 (MQ=255)
                                                              tGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGaa                                                                                 <  1:583269/148‑1 (MQ=255)
                                                              tGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGaa                                                                                 <  1:1522632/148‑1 (MQ=255)
                                                                 cgcTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAgg                                                                              <  2:3725932/148‑1 (MQ=255)
                                                                   cTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCg                                                                            <  1:1860608/148‑1 (MQ=255)
                                                                   cTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCg                                                                            >  2:873833/1‑148 (MQ=255)
                                                                    tGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGt                                                                           <  1:1817761/148‑1 (MQ=255)
                                                                    tGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGt                                                                           <  1:1365806/148‑1 (MQ=255)
                                                                     gcgcGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGtt                                                                          <  2:1564946/148‑1 (MQ=255)
                                                                      cgcgAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATAGAGAAGCCGAAAGGCGTTa                                                                         <  1:1001718/148‑1 (MQ=255)
                                                                       gcgAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTAt                                                                        <  2:637024/148‑1 (MQ=255)
                                                                          aaGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGt                                                                     >  1:2584274/1‑148 (MQ=255)
                                                                          aaGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGt                                                                     >  1:2944157/1‑148 (MQ=255)
                                                                           aGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTc                                                                    >  2:2302432/1‑148 (MQ=255)
                                                                             aCGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCa                                                                  >  2:2825141/1‑148 (MQ=255)
                                                                              cGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAg                                                                 >  2:3556269/1‑148 (MQ=255)
                                                                                 ttACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTACCTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTAc                                                              >  2:57422/1‑148 (MQ=255)
                                                                                 ttACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTAc                                                              >  2:1989522/1‑148 (MQ=255)
                                                                                  tACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTAcg                                                             >  1:2840116/1‑148 (MQ=255)
                                                                                   aCGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTAcgg                                                            <  2:1828348/148‑1 (MQ=255)
                                                                                   aCGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTAcgg                                                            <  1:1162904/148‑1 (MQ=255)
                                                                                   aCGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTAcgg                                                            <  1:1162872/148‑1 (MQ=255)
                                                                                   aCGAAACCATTATGGTTAACTGGAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTAcgg                                                            <  2:3615457/148‑1 (MQ=255)
                                                                                    cGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTAcggc                                                           >  1:2796344/1‑148 (MQ=255)
                                                                                      aaaCCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTAcggcgg                                                         >  1:1091134/1‑148 (MQ=255)
                                                                                       aaCCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGt                                                        <  1:277472/148‑1 (MQ=255)
                                                                                       aaCCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGAATAAGACACTTCAGACAGCCTCTACTTCGAGCCTGTAACTCTGGAAGATGTGCTGGAATTCGTGCGTATCGAGAATCCGAAAGTCGTTATCGTCCAGTACGGCGGt                                                        <  1:3417690/148‑1 (MQ=255)
                                                                                        aCCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTc                                                       <  2:1783739/148‑1 (MQ=255)
                                                                                           aTTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGa                                                    <  1:3053680/148‑1 (MQ=255)
                                                                                           aTTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGa                                                    >  1:431797/1‑148 (MQ=255)
                                                                                            ttATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGAc                                                   >  1:3483442/1‑148 (MQ=255)
                                                                                            ttATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGAc                                                   >  1:3483757/1‑148 (MQ=255)
                                                                                            ttATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGAc                                                   >  1:3483758/1‑148 (MQ=255)
                                                                                             tATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGAcc                                                  >  2:2157941/1‑148 (MQ=255)
                                                                                                ggTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCg                                               >  2:2500724/1‑148 (MQ=255)
                                                                                                ggTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCg                                               >  2:581105/1‑148 (MQ=255)
                                                                                                 gTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGc                                              <  1:3398663/148‑1 (MQ=255)
                                                                                                  ttAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCt                                             <  2:2079420/148‑1 (MQ=255)
                                                                                                  ttAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCt                                             <  2:2079421/148‑1 (MQ=255)
                                                                                                      cTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGaaa                                         >  2:3456950/1‑148 (MQ=255)
                                                                                                      cTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGaaa                                         >  2:795647/1‑148 (MQ=255)
                                                                                                      cTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGaaa                                         >  2:3456918/1‑148 (MQ=255)
                                                                                                      cTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGaaa                                         <  1:2764977/148‑1 (MQ=255)
                                                                                                       tGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAAc                                        >  1:3120530/1‑148 (MQ=255)
                                                                                                        gTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAACCt                                       >  2:202868/1‑148 (MQ=255)
                                                                                                         tAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTg                                      >  1:3532760/1‑148 (MQ=255)
                                                                                                          aaCCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTgg                                     <  1:1643555/148‑1 (MQ=255)
                                                                                                           aCCCGGAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGgc                                    >  1:924546/1‑148 (MQ=255)
                                                                                                            cccGGAAACCGTCTCCACCGACTACGACAATTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGAACGGCGGTCAGACCCCGCTGAAACTGgcg                                   >  1:3348454/1‑148 (MQ=255)
                                                                                                                gAAACCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGgcgcgcg                               >  2:2718077/1‑148 (MQ=255)
                                                                                                                 aaaCCGTCTCCACCGACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGgcgcgcgc                              >  2:2713633/1‑148 (MQ=255)
                                                                                                                  aaCCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGgcgcgcgcg                             <  1:2420879/148‑1 (MQ=255)
                                                                                                                  aaCCGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGgcgcgcgcg                             >  1:133390/1‑148 (MQ=255)
                                                                                                                    ccGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCt                           >  2:64663/1‑148 (MQ=255)
                                                                                                                     cGTCTCCACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTg                          >  1:3481813/1‑148 (MQ=255)
                                                                                                                          ccACCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAgc                     <  2:3441925/148‑1 (MQ=255)
                                                                                                                            aCCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAgctg                   >  1:2296623/1‑148 (MQ=255)
                                                                                                                            aCCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAgctg                   >  1:2297291/1‑148 (MQ=255)
                                                                                                                            aCCGACTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAgctg                   <  1:1546180/148‑1 (MQ=255)
                                                                                                                                 cTACGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGc              >  1:873363/1‑148 (MQ=255)
                                                                                                                                   aCGACACTTCCGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGACGCTGCTGGCGt            >  1:3251368/1‑148 (MQ=255)
                                                                                                                                             cGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTAta  <  2:3491897/148‑2 (MQ=255)
                                                                                                                                             cGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTAta  <  2:3223833/148‑2 (MQ=255)
                                                                                                                                             cGACAGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTAta  <  2:3491942/148‑2 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CGGCGGCCCGAACCGTATCGGTCAGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATC  >  CP009273/32514‑32802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑