Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,959,351 G→T 100% P77P (CCG→CCT)  trxA → thioredoxin 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,959,3510GT98.5% 850.6 / 2.8 262P77P (CCG→CCT) trxAthioredoxin 1
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (150/108);  total (152/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 262 total aligned reads displayed.

GGGCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGCC  >  CP009273/3959206‑3959494
                                                                                                                                                 |                                                                                                                                               
 ggCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTAct                                                                                                                                              <  1:2940985/148‑1 (MQ=255)
 ggCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTAct                                                                                                                                              <  1:3654462/148‑1 (MQ=255)
 ggCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTAct                                                                                                                                              >  1:3226097/1‑148 (MQ=255)
 ggCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTAct                                                                                                                                              >  2:1408910/1‑148 (MQ=255)
  gCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTActc                                                                                                                                             >  1:379947/1‑148 (MQ=255)
    agagTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTctg                                                                                                                                           <  1:2100235/148‑1 (MQ=255)
     gagTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTctgc                                                                                                                                          >  1:1758816/1‑148 (MQ=255)
      agTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTctgct                                                                                                                                         >  1:1446700/1‑148 (MQ=255)
               gTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCaa                                                                                                                                >  1:3592796/1‑148 (MQ=255)
               gTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCaa                                                                                                                                >  2:3033630/1‑148 (MQ=255)
                 ccGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCaaaa                                                                                                                              <  1:948754/148‑1 (MQ=255)
                     gCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACgg                                                                                                                          >  2:3252777/1‑148 (MQ=255)
                      cAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGt                                                                                                                         <  1:1877172/148‑1 (MQ=255)
                      cAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGt                                                                                                                         >  2:2527079/1‑148 (MQ=255)
                         aaTGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGaa                                                                                                                      >  2:32240/1‑148 (MQ=255)
                          aTGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAg                                                                                                                     >  2:3273216/1‑148 (MQ=255)
                            gATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTg                                                                                                                   <  2:2053618/148‑1 (MQ=255)
                            gATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTg                                                                                                                   >  2:213433/1‑148 (MQ=255)
                             aTCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTgg                                                                                                                  >  1:3040088/1‑148 (MQ=255)
                                 ccccGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTggcggc                                                                                                              >  1:638293/1‑148 (MQ=255)
                                    cGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAAc                                                                                                           >  1:717540/1‑148 (MQ=255)
                                         cTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAg                                                                                                      <  1:2649797/148‑1 (MQ=255)
                                         cTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAg                                                                                                      <  2:259837/148‑1 (MQ=255)
                                         cTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAg                                                                                                      <  2:3411697/148‑1 (MQ=255)
                                             aTGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTggg                                                                                                  <  1:2538532/148‑1 (MQ=255)
                                             aTGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTggg                                                                                                  <  1:3342718/148‑1 (MQ=255)
                                              tGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGt                                                                                                 >  2:1977640/1‑148 (MQ=255)
                                                 aaTCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCa                                                                                              <  1:3231342/148‑1 (MQ=255)
                                                 aaTCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCa                                                                                              <  1:3388105/148‑1 (MQ=255)
                                                         aCGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGGGCACTGGCGGCAACCAAAGTGGGTGCACTGTCTaa                                                                                      <  2:1744199/148‑1 (MQ=255)
                                                          cGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCACCCAAAGTGGGTGCACTGTCTaaa                                                                                     >  1:46065/1‑148 (MQ=255)
                                                          cGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTaaa                                                                                     >  1:670796/1‑148 (MQ=255)
                                                            aaTATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAgg                                                                                   >  1:851194/1‑148 (MQ=255)
                                                            aaTATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAgg                                                                                   <  2:2863253/148‑1 (MQ=255)
                                                              tatCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTc                                                                                 <  2:1845523/148‑1 (MQ=255)
                                                              tatCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTc                                                                                 <  2:1909600/148‑1 (MQ=255)
                                                               atCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCa                                                                                >  2:3007029/1‑148 (MQ=255)
                                                                tCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAg                                                                               >  2:2464421/1‑148 (MQ=255)
                                                                tCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAg                                                                               <  1:1692115/148‑1 (MQ=255)
                                                                 cAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGt                                                                              <  1:2726501/148‑1 (MQ=255)
                                                                         aCTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAagag                                                                      <  2:3425061/148‑1 (MQ=255)
                                                                         aCTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAagag                                                                      <  2:2250791/148‑1 (MQ=255)
                                                                         aCTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAagag                                                                      <  2:3011751/148‑1 (MQ=255)
                                                                          cTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGt                                                                     <  2:3640829/148‑1 (MQ=255)
                                                                           tGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGtt                                                                    <  2:3567414/148‑1 (MQ=255)
                                                                            gACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTc                                                                   >  1:3206427/1‑148 (MQ=255)
                                                                            gACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTc                                                                   >  1:240454/1‑148 (MQ=255)
                                                                             aCCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTcc                                                                  >  1:2998945/1‑148 (MQ=255)
                                                                             aCCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTcc                                                                  >  1:2843083/1‑148 (MQ=255)
                                                                             aCCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTcc                                                                  >  2:2204862/1‑148 (MQ=255)
                                                                             aCCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTcc                                                                  >  1:259433/1‑148 (MQ=255)
                                                                              ccGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCt                                                                 >  1:1139138/1‑148 (MQ=255)
                                                                                 ttGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGa                                                              >  1:492742/1‑148 (MQ=255)
                                                                                 ttGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGa                                                              >  2:1427938/1‑148 (MQ=255)
                                                                                   gCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACg                                                            <  1:570653/148‑1 (MQ=255)
                                                                                      aaaCTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTa                                                         >  2:878153/1‑148 (MQ=255)
                                                                                      aaaCTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTa                                                         <  1:1166077/148‑1 (MQ=255)
                                                                                        aCTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAAc                                                       >  2:1098874/1‑148 (MQ=255)
                                                                                        aCTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAAc                                                       >  1:2291940/1‑148 (MQ=255)
                                                                                         cTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAAcc                                                      <  1:201316/148‑1 (MQ=255)
                                                                                          tGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCt                                                     <  1:2765736/148‑1 (MQ=255)
                                                                                          tGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCt                                                     >  1:120677/1‑148 (MQ=255)
                                                                                           gAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTg                                                    <  1:684717/148‑1 (MQ=255)
                                                                                            aaCATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTgg                                                   <  2:12041/148‑1 (MQ=255)
                                                                                            aaCATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTgg                                                   >  2:1118396/1‑148 (MQ=255)
                                                                                              cATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCg                                                 >  1:2744530/1‑148 (MQ=255)
                                                                                                tCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTa                                               >  2:524264/1‑148 (MQ=255)
                                                                                                tCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTa                                               >  1:2272336/1‑148 (MQ=255)
                                                                                                tCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTa                                               <  1:1783280/148‑1 (MQ=255)
                                                                                                 cGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTaa                                              <  2:1921899/148‑1 (MQ=255)
                                                                                                   aTCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAgg                                            >  1:3428776/1‑148 (MQ=255)
                                                                                                     cAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGa                                          <  1:3694098/148‑1 (MQ=255)
                                                                                                     cAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGa                                          >  1:447569/1‑148 (MQ=255)
                                                                                                      aaaaCCCTGGCACTGCGCCTAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAACGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGaa                                         <  2:3790102/148‑1 (MQ=255)
                                                                                                        aaCCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGTAtt                                       <  1:765535/148‑1 (MQ=255)
                                                                                                        aaCCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAAtt                                       <  1:1187059/148‑1 (MQ=255)
                                                                                                          cccTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTc                                     <  1:223402/148‑1 (MQ=255)
                                                                                                          cccTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTc                                     >  1:1740300/1‑148 (MQ=255)
                                                                                                           ccTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCa                                    <  2:3689062/148‑1 (MQ=255)
                                                                                                             tGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATg                                  <  1:3756347/148‑1 (MQ=255)
                                                                                                             tGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATg                                  >  2:1071286/1‑148 (MQ=255)
                                                                                                              ggCACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGt                                 >  1:1079656/1‑148 (MQ=255)
                                                                                                                cACTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTc                               >  2:680180/1‑148 (MQ=255)
                                                                                                                  cTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCgg                             >  2:2968401/1‑148 (MQ=255)
                                                                                                                  cTGCGCCGAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCgg                             >  1:1017726/1‑148 (MQ=255)
                                                                                                                         gAAATATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGcccc                      >  2:43668/1‑148 (MQ=255)
                                                                                                                          aaaTATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCg                     >  2:2902735/1‑148 (MQ=255)
                                                                                                                          aaaTATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCg                     >  2:2646919/1‑148 (MQ=255)
                                                                                                                          aaaTATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCg                     >  1:3284288/1‑148 (MQ=255)
                                                                                                                          aaaTATGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAAAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCg                     >  2:1601839/1‑148 (MQ=255)
                                                                                                                            atatGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTc                   >  2:2296687/1‑148 (MQ=255)
                                                                                                                              atGGCATCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGc                 >  2:8917/1‑148 (MQ=255)
                                                                                                                                    tCCGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTaaaaa           <  2:1529450/148‑1 (MQ=255)
                                                                                                                                     ccGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGACTTTCATGTTCGGGTGCCCCGTCGCTAAAAAc          >  2:2106086/1‑148 (MQ=255)
                                                                                                                                     ccGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAAc          <  1:273804/148‑1 (MQ=255)
                                                                                                                                     ccGTGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAAc          >  2:3366907/1‑148 (MQ=255)
                                                                                                                                        tGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTgg       <  2:1244148/148‑1 (MQ=255)
                                                                                                                                        tGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTgg       >  1:3157397/1‑148 (MQ=255)
                                                                                                                                        tGGTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTgg       >  1:2670618/1‑148 (MQ=255)
                                                                                                                                          gTATCCCTACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGAc     >  2:3144667/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GGGCAGAGTGGTGCGGTCCGTGCAAAATGATCGCCCCGATTCTGGATGAAATCGCTGACGAATATCAGGGCAAACTGACCGTTGCAAAACTGAACATCGATCAAAACCCTGGCACTGCGCCGAAATATGGCATCCGTGGTATCCCGACTCTGCTGCTGTTCAAAAACGGTGAAGTGGCGGCAACCAAAGTGGGTGCACTGTCTAAAGGTCAGTTGAAAGAGTTCCTCGACGCTAACCTGGCGTAAGGGAATTTCATGTTCGGGTGCCCCGTCGCTAAAAACTGGACGCC  >  CP009273/3959206‑3959494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑