Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,967,474 G→T 100% A169A (GCG→GCT)  rffH → glucose‑1‑phosphate thymidylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,967,4740GT98.7% 756.9 / 0.4 233A169A (GCG→GCT) rffHglucose‑1‑phosphate thymidylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (113/117);  total (115/118)
Fisher's exact test for biased strand distribution p-value = 6.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 233 total aligned reads displayed.

GTCATGTTGCGGCGCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCGGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGCTCGGGCG  >  CP009273/3967329‑3967617
                                                                                                                                                 |                                                                                                                                               
gTCATGTTGCGGCGCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGt                                                                                                                                               >  2:110157/1‑148 (MQ=255)
   aTGTTGCGGCGCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGAc                                                                                                                                            >  2:265182/1‑148 (MQ=255)
     gTTGCGGCGCGCACCGCAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAGCGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCg                                                                                                                                          <  2:2839741/148‑1 (MQ=255)
     gTTGCGGCGCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCg                                                                                                                                          >  1:412198/1‑148 (MQ=255)
     gTTGCGGCGCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCg                                                                                                                                          <  2:921940/148‑1 (MQ=255)
         cggcgCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCt                                                                                                                                      <  2:548434/148‑1 (MQ=255)
          ggcgCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCtt                                                                                                                                     <  2:1678039/148‑1 (MQ=255)
           gcgcgcACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCttt                                                                                                                                    <  2:3220397/148‑1 (MQ=255)
            cgcgcACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTa                                                                                                                                   <  2:1603866/148‑1 (MQ=255)
            cgcgcACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTa                                                                                                                                   <  2:2165551/148‑1 (MQ=255)
                cACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCAGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTc                                                                                                                               >  1:1356824/1‑148 (MQ=255)
                   cGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTAc                                                                                                                            >  2:2873230/1‑148 (MQ=255)
                   cGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCGGTGACCGGGCTTTATTTCTAc                                                                                                                            >  1:3607693/1‑148 (MQ=255)
                     aaGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGa                                                                                                                          >  1:1953472/1‑148 (MQ=255)
                      aGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGAc                                                                                                                         >  2:3811273/1‑148 (MQ=255)
                       ggggCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACa                                                                                                                        >  2:2009971/1‑148 (MQ=255)
                        gggCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCAGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCGTTAGTTCTACGACAg                                                                                                                       >  2:2703273/1‑148 (MQ=255)
                             aCGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAg                                                                                                                  <  2:1394584/148‑1 (MQ=255)
                             aCGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAg                                                                                                                  <  2:1787755/148‑1 (MQ=255)
                              cGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGt                                                                                                                 <  2:1713063/148‑1 (MQ=255)
                                   tttGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTgg                                                                                                            <  1:747507/148‑1 (MQ=255)
                                   tttGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTgg                                                                                                            <  1:3789102/148‑1 (MQ=255)
                                   tttGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTgg                                                                                                            <  2:3215463/148‑1 (MQ=255)
                                   tttGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTgg                                                                                                            <  1:1546312/148‑1 (MQ=255)
                                          aTCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGc                                                                                                     >  1:3303631/1‑148 (MQ=255)
                                                  aTGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAgg                                                                                             >  1:3217997/1‑148 (MQ=255)
                                                    ggACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTg                                                                                           >  1:701438/1‑148 (MQ=255)
                                                     gACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGa                                                                                          <  1:1347616/148‑1 (MQ=255)
                                                       cccGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAg                                                                                        <  2:1328090/148‑1 (MQ=255)
                                                       cccGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAg                                                                                        <  2:1288942/148‑1 (MQ=255)
                                                        ccGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGc                                                                                       >  2:2088983/1‑148 (MQ=255)
                                                            aaCGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTc                                                                                   >  2:1866947/1‑148 (MQ=255)
                                                            aaCGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTc                                                                                   >  1:3573995/1‑148 (MQ=255)
                                                                cTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAg                                                                               <  2:3176288/148‑1 (MQ=255)
                                                                 tttGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCACAGCAGGTGAAGCCGTCGGAGc                                                                              >  1:1941820/1‑148 (MQ=255)
                                                                 tttGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGc                                                                              >  1:665570/1‑148 (MQ=255)
                                                                 tttGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGACGGAGc                                                                              >  2:3055689/1‑148 (MQ=255)
                                                                  ttGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCg                                                                             >  2:955755/1‑148 (MQ=255)
                                                                   tGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCgt                                                                            <  2:34346/148‑1 (MQ=255)
                                                                    ggCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCgtg                                                                           <  2:93777/148‑1 (MQ=255)
                                                                        tggtggAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGa                                                                       >  1:1383418/1‑148 (MQ=255)
                                                                        tggtggAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGa                                                                       >  2:1172751/1‑148 (MQ=255)
                                                                         ggtggAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGaa                                                                      <  1:853900/148‑1 (MQ=255)
                                                                             gAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTgg                                                                  >  1:2793892/1‑148 (MQ=255)
                                                                             gAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTgg                                                                  >  1:3818521/1‑148 (MQ=255)
                                                                               gTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGgag                                                                <  1:1160984/148‑1 (MQ=255)
                                                                                 ttGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGAt                                                              <  2:1778503/148‑1 (MQ=255)
                                                                                 ttGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGAt                                                              <  1:2089026/148‑1 (MQ=255)
                                                                                     cgacAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTAcc                                                          <  1:938409/148‑1 (MQ=255)
                                                                                     cgacAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTAcc                                                          >  1:183052/1‑148 (MQ=255)
                                                                                       acAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTc                                                        >  1:3125850/1‑148 (MQ=255)
                                                                                             tCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCaa                                                  <  2:1777405/148‑1 (MQ=255)
                                                                                             tCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCaa                                                  <  1:2873230/148‑1 (MQ=255)
                                                                                              ccGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGTAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAAc                                                 >  2:1608416/1‑148 (MQ=255)
                                                                                              ccGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAAc                                                 >  1:2410992/1‑148 (MQ=255)
                                                                                               cgcgCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAAcc                                                <  1:1444846/148‑1 (MQ=255)
                                                                                               cgcgCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAAcc                                                <  1:1635654/148‑1 (MQ=255)
                                                                                                  gcTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGa                                             <  1:3732307/148‑1 (MQ=255)
                                                                                                  gcTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGa                                             <  1:2173190/148‑1 (MQ=255)
                                                                                                   cTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGAt                                            >  1:1168609/1‑148 (MQ=255)
                                                                                                     aTCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGt                                          >  1:2773356/1‑148 (MQ=255)
                                                                                                      tctcGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTa                                         <  1:1255456/148‑1 (MQ=255)
                                                                                                      tctcGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGACTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTa                                         >  1:892582/1‑148 (MQ=255)
                                                                                                       ctcGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTAc                                        >  2:228978/1‑148 (MQ=255)
                                                                                                        tcGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGTTGTAcc                                       >  2:1485119/1‑148 (MQ=255)
                                                                                                         cGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCt                                      >  1:1973836/1‑148 (MQ=255)
                                                                                                          gCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTc                                     >  1:864823/1‑148 (MQ=255)
                                                                                                           cTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCg                                    <  2:372925/148‑1 (MQ=255)
                                                                                                            tGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGa                                   <  2:392103/148‑1 (MQ=255)
                                                                                                            tGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGa                                   <  2:3590547/148‑1 (MQ=255)
                                                                                                            tGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGa                                   <  2:1463435/148‑1 (MQ=255)
                                                                                                             ggAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAg                                  <  1:180273/148‑1 (MQ=255)
                                                                                                             ggAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAg                                  >  2:2578814/1‑148 (MQ=255)
                                                                                                             ggAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAg                                  <  1:180288/148‑1 (MQ=255)
                                                                                                              gaagaaAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAgg                                 >  1:1033522/1‑148 (MQ=255)
                                                                                                               aagaaAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGc                                <  2:21871/148‑1 (MQ=255)
                                                                                                                agaaAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCg                               <  1:2640199/148‑1 (MQ=255)
                                                                                                                 gaaAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCgg                              >  1:2494820/1‑148 (MQ=255)
                                                                                                                 gaaAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCgg                              >  1:575000/1‑148 (MQ=255)
                                                                                                                 gaaAAGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCgg                              >  1:2899095/1‑148 (MQ=255)
                                                                                                                  aaaaGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCggg                             >  1:2896823/1‑148 (MQ=255)
                                                                                                                  aaaaGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCggg                             >  2:3524535/1‑148 (MQ=255)
                                                                                                                  aaaaGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCggg                             <  2:1524320/148‑1 (MQ=255)
                                                                                                                   aaaGCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGc                            >  2:2453655/1‑148 (MQ=255)
                                                                                                                      gCCAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTCCCTCCAGCATCCCGCTGTCCCTCGAGGCGGGCCAc                         >  1:2441728/1‑148 (MQ=255)
                                                                                                                        cAAAACAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCt                       <  2:665570/148‑1 (MQ=255)
                                                                                                                         aaaaCAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTg                      <  1:422417/148‑1 (MQ=255)
                                                                                                                            aCAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGAcc                   >  1:1573292/1‑148 (MQ=255)
                                                                                                                            aCAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGAcc                   >  1:2960168/1‑148 (MQ=255)
                                                                                                                             cAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCg                  <  2:701589/148‑1 (MQ=255)
                                                                                                                             cAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCg                  <  2:701388/148‑1 (MQ=255)
                                                                                                                             cAGCCGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGAAAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCg                  <  2:2393668/148‑1 (MQ=255)
                                                                                                                                 cGAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGa              >  2:3358626/1‑148 (MQ=255)
                                                                                                                                  gAAGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGaa             >  2:129284/1‑148 (MQ=255)
                                                                                                                                   aaGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAAc            >  2:1011913/1‑148 (MQ=255)
                                                                                                                                   aaGTCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAAc            <  1:1677841/148‑1 (MQ=255)
                                                                                                                                      tCAAACTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGc         >  2:3361815/1‑148 (MQ=255)
                                                                                                                                         aaCTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGCTCg      >  1:2253093/1‑148 (MQ=255)
                                                                                                                                         aaCTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGCTCg      >  1:1421596/1‑148 (MQ=255)
                                                                                                                                          aCTGGGCTGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGCTCgg     >  2:1216382/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GTCATGTTGCGGCGCGCACCGAAGGGGCGACGGTTTTTGGCTATCAGGTGATGGACCCGGAACGCTTTGGCGTGGTGGAGTTTGACGACAATTTCCGCGCTATCTCGCTGGAAGAAAAGCCAAAACAGCCGAAGTCAAACTGGGCGGTGACCGGGCTTTATTTCTACGACAGTAAAGTCGTGGAGTACGCAAAGCAGGTGAAGCCGTCGGAGCGTGGTGAACTGGAGATTACCTCCATCAACCAGATGTACCTCGAGGCGGGCAACCTGACCGTTGAACTGCTCGGGCG  >  CP009273/3967329‑3967617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑