Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,996,346 T→A 100% I70F (ATC→TTC)  yigG ← PRK11371 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,996,3460TA98.5% 1214.7 / 3.6 373I70F (ATC→TTC) yigGPRK11371 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (2/4);  new base A (203/164);  total (205/168)
Fisher's exact test for biased strand distribution p-value = 4.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 384 total aligned reads displayed.

AGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAAAATAAA  >  CP009273/3996201‑3996490
                                                                                                                                                 |                                                                                                                                                
 gCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCGAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAgaagaa                                                                                                                                               <  1:539961/148‑1 (MQ=255)
 gCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAgaagaa                                                                                                                                               <  1:704801/148‑1 (MQ=255)
  cATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGaaa                                                                                                                                              <  2:490737/148‑1 (MQ=255)
  cATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGaaa                                                                                                                                              <  1:3507049/148‑1 (MQ=255)
  cATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGaaa                                                                                                                                              >  2:35892/1‑148 (MQ=255)
    ttAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAAcc                                                                                                                                            <  2:1658039/148‑1 (MQ=255)
     tAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCa                                                                                                                                           >  1:1055918/1‑148 (MQ=255)
     tAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCa                                                                                                                                           >  1:3305277/1‑148 (MQ=255)
       gCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACt                                                                                                                                         <  2:280674/148‑1 (MQ=255)
        cATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTa                                                                                                                                        <  2:1825399/148‑1 (MQ=255)
         aTACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTAt                                                                                                                                       <  2:1362397/148‑1 (MQ=255)
           aCTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCa                                                                                                                                     >  1:3330115/1‑148 (MQ=255)
            cTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCAc                                                                                                                                    <  2:1859502/148‑1 (MQ=255)
              gAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACgg                                                                                                                                  >  1:2433730/1‑148 (MQ=255)
                aaGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAg                                                                                                                                >  1:1398233/1‑148 (MQ=255)
                aaGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGACTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAg                                                                                                                                >  1:789219/1‑148 (MQ=255)
                 aGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGt                                                                                                                               >  2:3105069/1‑148 (MQ=255)
                 aGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGt                                                                                                                               >  2:3624189/1‑148 (MQ=255)
                 aGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGt                                                                                                                               >  1:1982970/1‑148 (MQ=255)
                 aGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCAGAAAAGGCAAAATCTTAGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAATTTTTTATATAGAAGAAACAACTATAACGGatt                                                                                                                               >  1:3627805/1‑146 (MQ=255)
                   tGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCg                                                                                                                             >  2:3157184/1‑148 (MQ=255)
                   tGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCg                                                                                                                             >  2:85430/1‑148 (MQ=255)
                        aaaTAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGc                                                                                                                        >  1:2536749/1‑148 (MQ=255)
                         aaTAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCa                                                                                                                       >  2:424414/1‑148 (MQ=255)
                         aaTAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCa                                                                                                                       >  2:3700426/1‑148 (MQ=255)
                          aTAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCaa                                                                                                                      <  1:1342268/148‑1 (MQ=255)
                           tAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAAt                                                                                                                     >  2:1176787/1‑148 (MQ=255)
                           tAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAAt                                                                                                                     >  2:1686733/1‑148 (MQ=255)
                            aaCAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAAtt                                                                                                                    >  2:235619/1‑148 (MQ=255)
                            aaCAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAAtt                                                                                                                    >  1:381869/1‑148 (MQ=255)
                              cAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCCATTCa                                                                                                                  >  2:102277/1‑148 (MQ=255)
                              cAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCa                                                                                                                  <  2:50760/148‑1 (MQ=255)
                               aaaTCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCACTTCAt                                                                                                                 >  1:1195029/1‑148 (MQ=255)
                               aaaTCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCAt                                                                                                                 <  1:2334284/148‑1 (MQ=255)
                                 aTCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGt                                                                                                               >  2:2654529/1‑148 (MQ=255)
                                   cAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTtg                                                                                                             <  1:2447858/148‑1 (MQ=255)
                                    aaaaaaaTCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTtga                                                                                                            <  1:2948988/148‑1 (MQ=255)
                                    aaaaaaaTCATCGAACCAGTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTtga                                                                                                            <  2:2052507/148‑1 (MQ=255)
                                     aaaaaaTCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTtgat                                                                                                           <  1:1702133/148‑1 (MQ=255)
                                     aaaaaaTCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTtgat                                                                                                           >  2:2215144/1‑148 (MQ=255)
                                     aaaaaaTCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTtgat                                                                                                           >  2:1246507/1‑148 (MQ=255)
                                      aaaaaTCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTtgatg                                                                                                          >  2:754734/1‑148 (MQ=255)
                                       aaaaTCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTtgatga                                                                                                         >  2:2715959/1‑148 (MQ=255)
                                         aaTCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCGGGCAATTCATGTTGATGACg                                                                                                       >  1:3558125/1‑148 (MQ=255)
                                           tcatcGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACgag                                                                                                     <  2:2093738/148‑1 (MQ=255)
                                            catcGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACgaga                                                                                                    >  2:654595/1‑148 (MQ=255)
                                             atcGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGAt                                                                                                   <  1:310234/148‑1 (MQ=255)
                                               cGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATTTTGTTTAAGAGATaa                                                                                                 >  1:1426746/1‑148 (MQ=255)
                                               cGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATaa                                                                                                 >  2:1421755/1‑148 (MQ=255)
                                               cGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATaa                                                                                                 >  1:1755629/1‑148 (MQ=255)
                                                      ttGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTa                                                                                          <  1:1608623/148‑1 (MQ=255)
                                                          cTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGAt                                                                                      <  2:1912717/148‑1 (MQ=255)
                                                           tGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATg                                                                                     <  1:888628/148‑1 (MQ=255)
                                                             aaCAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGt                                                                                   >  1:1547750/1‑148 (MQ=255)
                                                                   cAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGAc                                                                             <  2:1280553/148‑1 (MQ=255)
                                                                   cAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGAc                                                                             >  2:2795189/1‑148 (MQ=255)
                                                                       aTCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACtata                                                                         <  1:477008/148‑1 (MQ=255)
                                                                       aTCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTAATGTTGATGACGAGATAATGGAGTACGAAGGTAGAGACtata                                                                         >  2:2563741/1‑148 (MQ=255)
                                                                                 aTCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGc                                                               >  2:3205786/1‑148 (MQ=255)
                                                                                    aTTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTg                                                            <  1:206159/148‑1 (MQ=255)
                                                                                    aTTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTg                                                            <  1:2852959/148‑1 (MQ=255)
                                                                                     ttGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTAATTTTTATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGc                                                           >  1:1115995/1‑148 (MQ=255)
                                                                                       gtgATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCtt                                                         <  1:1033486/148‑1 (MQ=255)
                                                                                        tgatgaTAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTtc                                                        <  1:646217/148‑1 (MQ=255)
                                                                                          atgatAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCCATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTtctc                                                      >  1:2127962/1‑148 (MQ=255)
                                                                                           tgatAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGTGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTcc                                                     >  1:1913097/1‑148 (MQ=255)
                                                                                            gatAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAAATAATGTTTATTACGAGATAATGGAGTAACATGGTATAGACTATAACAAGAAAACCTGCTTCTCCa                                                    >  1:2621916/1‑148 (MQ=255)
                                                                                               agagATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCg                                                 <  1:86320/148‑1 (MQ=255)
                                                                                                gagaTGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAATCCTGCTTCTCCATCGt                                                >  2:2618172/1‑148 (MQ=255)
                                                                                                 agaTGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAAATCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAAAAAGAAAGCCAGCTTCTCCATCGtt                                               >  1:2829267/1‑148 (MQ=255)
                                                                                                   atgatATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTaa                                             <  2:399157/148‑1 (MQ=255)
                                                                                                     gatATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTaaaa                                           <  2:2315582/148‑1 (MQ=255)
                                                                                                      atatatACTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTaaaaa                                          <  2:1159893/148‑1 (MQ=255)
                                                                                                       tatataCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTaaaaaa                                         >  2:2033234/1‑148 (MQ=255)
                                                                                                        atataCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTTTTGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTaaaaaac                                        >  2:2891262/1‑147 (MQ=255)
                                                                                                        atataCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAg                                        <  2:2985351/148‑1 (MQ=255)
                                                                                                         tataCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGa                                       <  2:1623082/148‑1 (MQ=255)
                                                                                                          ataCTGCTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGat                                      >  2:1057465/1‑148 (MQ=255)
                                                                                                                cTAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAg                                <  1:373113/148‑1 (MQ=255)
                                                                                                                 tAATGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGTTAATAAGa                               >  2:346946/1‑148 (MQ=255)
                                                                                                                  aaTGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTGATGTTGTTGTCGAGATAATTGAGTAGGTTGGTAGGGGGTATAAGAAGGAAGCCTGCTTCACCATCGTTAAAAAAGATAATAAGaa                              >  1:2776344/1‑148 (MQ=255)
                                                                                                                   aTGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGaaa                             <  1:3092855/148‑1 (MQ=255)
                                                                                                                    tGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAg                            >  2:1486682/1‑148 (MQ=255)
                                                                                                                    tGTACCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTAATGTTGATGAAGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAg                            >  2:339550/1‑148 (MQ=255)
                                                                                                                       aCCAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCa                         <  1:45393/148‑1 (MQ=255)
                                                                                                                        ccAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCaa                        >  2:2411216/1‑148 (MQ=255)
                                                                                                                        ccAAAAACATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTAATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCaa                        >  1:1484937/1‑148 (MQ=255)
                                                                                                                          aaaaaCATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCaaaa                      <  1:35892/148‑1 (MQ=255)
                                                                                                                            aaaCATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCATCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAAt                    >  2:2998142/1‑148 (MQ=255)
                                                                                                                             aaCATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATg                   >  2:144355/1‑148 (MQ=255)
                                                                                                                               cATAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGaa                 <  2:3432280/148‑1 (MQ=255)
                                                                                                                                aTAAGTTTTTATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATTaaa                >  2:1952399/1‑148 (MQ=255)
                                                                                                                                  aattttttATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAAtt              <  2:978750/145‑1 (MQ=255)
                                                                                                                                     tttttATATAGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATttat           <  1:1743894/148‑1 (MQ=255)
                                                                                                                                         tatataGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTaaa       <  2:1751570/148‑1 (MQ=255)
                                                                                                                                          atataGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTaaaa      >  1:1250282/1‑148 (MQ=255)
                                                                                                                                             taGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAaaataa   <  1:3048578/148‑1 (MQ=255)
                                                                                                                                             taGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAaaataa   >  1:2243617/1‑148 (MQ=255)
                                                                                                                                             taGAAGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAaaataa   <  1:129076/148‑1 (MQ=255)
                                                                                                                                              agaagaAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAaaataaa  >  2:1788680/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGCATTAGCATACTGAAAGTGGAAAAATAACAAATCAAAAAAATCATCGAACCATTGCCTGAACAGGCAAAATCTTCGGCTATCATTGTGATGATAGAGATGATATATACTGCTAATGTACCAAAAACATAAGTTTTTATATAGATGAAACCACTATCACGGAGTCGCTGGCAATTCATGTTGATGACGAGATAATGGAGTACGATGGTAGAGACTATAACAAGAAAGCCTGCTTCTCCATCGTTAAAAAAGATAATAAGAAAGGCAAAAATGAAATTTATTAAAATAAA  >  CP009273/3996201‑3996490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑