Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 314,566 C→A 100% D383Y (GAT→TAT)  rclA ← reactive chlorine stress species (RCS) resistance protein; pyridine nucleotide‑dependent disulfide oxidoreductase family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273314,5660CA98.5% 624.3 / 1.1 198D383Y (GAT→TAT) rclAreactive chlorine stress species (RCS) resistance protein; pyridine nucleotide‑dependent disulfide oxidoreductase family
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base A (106/89);  total (109/89)
Fisher's exact test for biased strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 209 total aligned reads displayed.

TCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTCTCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGT  >  CP009273/314421‑314710
                                                                                                                                                 |                                                                                                                                                
tCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATaaa                                                                                                                                                <  1:2020466/148‑1 (MQ=255)
  gCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATaaaca                                                                                                                                              <  1:3762256/148‑1 (MQ=255)
   cTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATaaacaa                                                                                                                                             <  1:2150752/148‑1 (MQ=255)
    tCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAAt                                                                                                                                            <  2:770771/148‑1 (MQ=255)
    tCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAAt                                                                                                                                            <  2:3258446/148‑1 (MQ=255)
     cATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATc                                                                                                                                           >  2:2770475/1‑148 (MQ=255)
      aTCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCg                                                                                                                                          <  1:3003200/148‑1 (MQ=255)
      aTCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCAACTCGAGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATAATAAACAATCg                                                                                                                                          >  1:2976678/1‑148 (MQ=255)
          aCGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCttt                                                                                                                                      <  1:776081/148‑1 (MQ=255)
            ggATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTa                                                                                                                                    <  1:1860142/148‑1 (MQ=255)
             gATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTaa                                                                                                                                   <  2:2219410/148‑1 (MQ=255)
              aTGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAAt                                                                                                                                  <  1:2820865/148‑1 (MQ=255)
                  gTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATAccc                                                                                                                              >  2:2494421/1‑148 (MQ=255)
                    aaaTATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCa                                                                                                                            >  2:2068964/1‑148 (MQ=255)
                    aaaTATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCa                                                                                                                            >  2:515618/1‑148 (MQ=255)
                       tatCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGa                                                                                                                         >  1:244713/1‑148 (MQ=255)
                       tatCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGa                                                                                                                         >  2:1844742/1‑148 (MQ=255)
                       tatCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGa                                                                                                                         >  2:108876/1‑148 (MQ=255)
                         tCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGt                                                                                                                       <  1:953401/148‑1 (MQ=255)
                         tCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGt                                                                                                                       <  2:761162/148‑1 (MQ=255)
                           tGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTAt                                                                                                                     <  2:1325449/148‑1 (MQ=255)
                            gATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTAtc                                                                                                                    >  2:637517/1‑148 (MQ=255)
                                gcgTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTAtaattc                                                                                                                <  1:267321/148‑1 (MQ=255)
                                   tAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATtcatc                                                                                                             <  2:3671116/148‑1 (MQ=255)
                                      tatGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCAct                                                                                                          >  1:1039594/1‑148 (MQ=255)
                                          cTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTgg                                                                                                      >  1:1943891/1‑148 (MQ=255)
                                           tataAGTCAGCCCGTCATCCATCACAATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTgtc                                                                                                     <  2:2616298/148‑3 (MQ=255)
                                            ataAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCa                                                                                                    >  2:2257363/1‑148 (MQ=255)
                                             taAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCAc                                                                                                   >  1:1057077/1‑148 (MQ=255)
                                              aaGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCAcg                                                                                                  >  2:2232935/1‑148 (MQ=255)
                                              aaGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCAcg                                                                                                  <  1:833361/148‑1 (MQ=255)
                                                ggCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCAcgcg                                                                                                >  2:440914/1‑148 (MQ=255)
                                                   aGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGaa                                                                                             <  2:1224581/148‑1 (MQ=255)
                                                    gCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAAt                                                                                            >  1:3487500/1‑148 (MQ=255)
                                                    gCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAAt                                                                                            >  1:1589452/1‑148 (MQ=255)
                                                    gCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAAt                                                                                            <  1:736047/148‑1 (MQ=255)
                                                     cccGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAAtt                                                                                           >  1:1652209/1‑148 (MQ=255)
                                                     cccGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAAtt                                                                                           <  2:3024890/148‑1 (MQ=255)
                                                     cccGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAAtt                                                                                           >  2:3136256/1‑148 (MQ=255)
                                                     cccGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAAtt                                                                                           >  1:381349/1‑148 (MQ=255)
                                                      ccGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATAACCCACGAGTATCATTCATCACTCTGGCACGCGGAATTg                                                                                          <  1:1537022/148‑1 (MQ=255)
                                                             ccatcACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCtcc                                                                                   >  2:2663424/1‑146 (MQ=255)
                                                             ccatcACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTAc                                                                                   >  2:2663574/1‑148 (MQ=255)
                                                             ccatcACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTAc                                                                                   >  2:671210/1‑148 (MQ=255)
                                                             ccatcACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTAc                                                                                   >  2:2359115/1‑148 (MQ=255)
                                                                 cACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGc                                                                               >  2:3735408/1‑148 (MQ=255)
                                                                 cACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGc                                                                               >  1:3698989/1‑148 (MQ=255)
                                                                  aCCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCa                                                                              >  1:2786794/1‑148 (MQ=255)
                                                                   ccATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCaa                                                                             >  2:215306/1‑148 (MQ=255)
                                                                     aTTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATg                                                                           <  1:2240681/148‑1 (MQ=255)
                                                                     aTTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATg                                                                           <  2:72502/148‑1 (MQ=255)
                                                                      ttttCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGt                                                                          >  1:363886/1‑148 (MQ=255)
                                                                       tttCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTc                                                                         >  2:3307016/1‑148 (MQ=255)
                                                                       tttCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTc                                                                         >  1:1471431/1‑148 (MQ=255)
                                                                           aCTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCACTGTcacca                                                                     >  1:3781997/1‑148 (MQ=255)
                                                                            cTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTcaccac                                                                    >  2:2313097/1‑148 (MQ=255)
                                                                                atTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGa                                                                >  1:3594932/1‑148 (MQ=255)
                                                                                 ttGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGaa                                                               <  2:1039594/148‑1 (MQ=255)
                                                                                 ttGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGaa                                                               <  2:2948701/148‑1 (MQ=255)
                                                                                  tGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAat                                                              >  1:1460078/1‑148 (MQ=255)
                                                                                  tGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAat                                                              >  1:2610030/1‑148 (MQ=255)
                                                                                  tGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAat                                                              >  2:3442226/1‑148 (MQ=255)
                                                                                  tGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAat                                                              <  1:3176435/148‑1 (MQ=255)
                                                                                  tGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAat                                                              >  1:86779/1‑148 (MQ=255)
                                                                                    atcatcTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAatat                                                            >  2:2354830/1‑148 (MQ=255)
                                                                                    atcatcTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAatat                                                            >  2:345200/1‑148 (MQ=255)
                                                                                     tcatcTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATc                                                           >  1:3458049/1‑148 (MQ=255)
                                                                                     tcatcTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATc                                                           <  2:485404/148‑1 (MQ=255)
                                                                                      catcTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCa                                                          <  2:2111530/148‑1 (MQ=255)
                                                                                      catcTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCa                                                          <  2:2528643/148‑1 (MQ=255)
                                                                                       atcTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGTAGCTACAGGCAATGTCACCACCTGAATATCAg                                                         <  1:3548690/148‑1 (MQ=255)
                                                                                       atcTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAg                                                         >  1:269555/1‑148 (MQ=255)
                                                                                        tctcGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGc                                                        <  2:2854275/148‑1 (MQ=255)
                                                                                         ctcGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGca                                                       >  2:572760/1‑148 (MQ=255)
                                                                                         ctcGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGca                                                       >  1:1316373/1‑148 (MQ=255)
                                                                                               ggAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCActc                                                 <  1:637517/148‑1 (MQ=255)
                                                                                               ggAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCActc                                                 <  1:516375/148‑1 (MQ=255)
                                                                                                   tCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCActctctc                                             <  2:70236/148‑1 (MQ=255)
                                                                                                    cAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCActctctct                                            >  2:1954654/1‑148 (MQ=255)
                                                                                                      acacacAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTCTCTgg                                          <  2:244713/148‑1 (MQ=255)
                                                                                                      acacacAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATAACAGCACCACTCTCTCTgg                                          >  1:91067/1‑148 (MQ=255)
                                                                                                       cacacaGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTCTCTGGc                                         <  2:1698160/148‑1 (MQ=255)
                                                                                                          acaGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTCTCTGGCTTg                                      <  1:3424405/148‑1 (MQ=255)
                                                                                                            agcagTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTCTCTGGCTTGtt                                    >  1:3730290/1‑148 (MQ=255)
                                                                                                            agcagTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTCTCTGGCTTGtt                                    <  2:1652209/148‑1 (MQ=255)
                                                                                                               agTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTCTCTGGCTTGttctt                                 <  2:3667195/148‑1 (MQ=255)
                                                                                                               agTGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTCTCTGGCTTGttctt                                 <  1:317252/148‑1 (MQ=255)
                                                                                                                 tGATGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTCTCTGGCTTGTtcttct                               >  1:1404482/1‑148 (MQ=255)
                                                                                                                   aTGCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCAACGAGTATCATTCATCACTCTGGCACGAGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATAAGAACCACTCTCTCTGGCTTGTTCTTCTGt                             >  2:3663777/1‑148 (MQ=255)
                                                                                                                     gCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTCTCTGGCTTGTTCTTCTGTCa                           <  2:1343236/148‑1 (MQ=255)
                                                                                                                     gCCCCTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAAGACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTCTCTGGCTTGTTCTTCTGTCa                           <  2:3512139/148‑1 (MQ=255)
                                                                                                                         cTAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTCTCTGGCTTGTTCTTCTGTCATAcc                       >  2:3018917/1‑148 (MQ=255)
                                                                                                                          tAACATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTCTCTGGCTTGTTCTTCTGTCATACCa                      >  2:653548/1‑148 (MQ=255)
                                                                                                                            aCATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTCTCTGGCTTGTTCTTCTGTCATACCAAc                    >  1:3666195/1‑148 (MQ=255)
                                                                                                                            aCATACGTTGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTCTCTGGCTTGTTCTTCTGTCATACCAAc                    >  1:1775479/1‑148 (MQ=255)
                                                                                                                                   ttGGGTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTCTCTGGCTTGTTCTTCTGTCATACCAACCCTGGAc             <  2:18961/148‑1 (MQ=255)
                                                                                                                                      ggTTTTATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTCTCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAgg          <  1:440914/148‑1 (MQ=255)
                                                                                                                                         tttATTATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTCTCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGc       >  1:3503623/1‑148 (MQ=255)
                                                                                                                                         tttATAATAAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTCTCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGc       >  1:733068/1‑148 (MQ=255)
                                                                                                                                              tataAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTCTCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGgtgt  >  2:3781066/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCGCTCATCGACGGATGAGTAAATATCTGATCGCGTAATATGCTATAAGGCAGCCCGGCATCCATCACCATTTTCACTATATTGATCATCTCGTGGGAGTCAACACACAGCAGTGATGCCCCTAACATACGTTGGGTTTTATTATCAACAATCGCTTTTAATACCCCACGAGTATCATTCATCACTCTGGCACGCGGAATTGCAGCTACAGGCAATGTCACCACCTGAATATCAGCACCACTCTCTCTGGCTTGTTCTTCTGTCATACCAACCCTGGACAGGGGCGGTGT  >  CP009273/314421‑314710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑