Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,046,172 A→T 100% K256N (AAA→AAT)  hemN → coproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,046,1720AT99.4% 549.4 / ‑4.4 167K256N (AAA→AAT) hemNcoproporphyrinogen III oxidase, SAM and NAD(P)H dependent, oxygen‑independent
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (99/67);  total (99/68)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 167 total aligned reads displayed.

TCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGC  >  CP009273/4046027‑4046316
                                                                                                                                                 |                                                                                                                                                
tCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGa                                                                                                                                                >  1:2858014/1‑148 (MQ=255)
 cGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCTCAAAATCAATGAt                                                                                                                                               <  2:1726770/148‑1 (MQ=255)
 cGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGAt                                                                                                                                               >  1:611702/1‑148 (MQ=255)
 cGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGAt                                                                                                                                               >  1:611723/1‑148 (MQ=255)
    ccTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCt                                                                                                                                            >  2:2232168/1‑148 (MQ=255)
     cTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTg                                                                                                                                           >  1:3760352/1‑148 (MQ=255)
       gATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGAc                                                                                                                                         <  2:3641454/148‑1 (MQ=255)
              ggCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGa                                                                                                                                  <  2:3556756/148‑1 (MQ=255)
               gCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCCGACCTGCCGAg                                                                                                                                 >  1:339228/1‑148 (MQ=255)
                   gCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCg                                                                                                                             >  1:2899305/1‑148 (MQ=255)
                   gCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCg                                                                                                                             <  2:2372122/148‑1 (MQ=255)
                        aaCAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCgcagca                                                                                                                        >  2:2380378/1‑148 (MQ=255)
                        aaCAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCgcagca                                                                                                                        >  1:2661125/1‑148 (MQ=255)
                        aaCAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCgcagca                                                                                                                        >  2:2381835/1‑148 (MQ=255)
                           aGACGCCGGAGAGTTTCGCCTTTACCCTGACACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCaaaa                                                                                                                     <  2:2711956/148‑1 (MQ=255)
                             aCGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAAc                                                                                                                   >  2:2522686/1‑148 (MQ=255)
                                   gagaGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGata                                                                                                             >  1:1912480/1‑148 (MQ=255)
                                   gagaGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGata                                                                                                             >  1:1912435/1‑148 (MQ=255)
                                   gagaGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGata                                                                                                             >  1:77108/1‑148 (MQ=255)
                                    agagTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGatat                                                                                                            >  2:1929790/1‑148 (MQ=255)
                                      agTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATcc                                                                                                          <  1:1810360/148‑1 (MQ=255)
                                      agTTTCGCCTTGACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATcc                                                                                                          <  2:2988001/148‑1 (MQ=255)
                                        tttCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTg                                                                                                        >  1:3227825/1‑148 (MQ=255)
                                         ttCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGc                                                                                                       <  1:2829141/148‑1 (MQ=255)
                                         ttCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGc                                                                                                       <  1:2829149/148‑1 (MQ=255)
                                                ttACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAAc                                                                                                >  1:476920/1‑148 (MQ=255)
                                                 tACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAAcc                                                                                               <  2:83287/148‑1 (MQ=255)
                                                  aCCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGTAGCAAAAACTCGATATCCTGCAGGAAACCa                                                                                              <  1:1901770/148‑1 (MQ=255)
                                                  aCCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCa                                                                                              <  2:2949339/148‑1 (MQ=255)
                                                    ccTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATc                                                                                            >  1:181245/1‑148 (MQ=255)
                                                       gAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGcc                                                                                         <  2:1317428/148‑1 (MQ=255)
                                                       gAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGcc                                                                                         <  1:764968/148‑1 (MQ=255)
                                                          aCGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTc                                                                                      <  2:3425909/148‑1 (MQ=255)
                                                           cGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTcc                                                                                     <  2:2071129/148‑1 (MQ=255)
                                                             tgtgGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTg                                                                                   >  1:2375774/1‑148 (MQ=255)
                                                                   ggAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCaa                                                                             <  1:1678764/148‑1 (MQ=255)
                                                                      gCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCg                                                                          >  2:1705086/1‑148 (MQ=255)
                                                                       cTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCgg                                                                         >  1:1708025/1‑148 (MQ=255)
                                                                          aaCCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCt                                                                      >  1:324277/1‑148 (MQ=255)
                                                                           aCCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTa                                                                     <  2:2640063/148‑1 (MQ=255)
                                                                              ccGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCa                                                                  >  1:2500609/1‑148 (MQ=255)
                                                                                gATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGt                                                                <  2:1130051/148‑1 (MQ=255)
                                                                                gATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGt                                                                <  2:2479360/148‑1 (MQ=255)
                                                                                    gTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTAt                                                            <  1:2704872/148‑1 (MQ=255)
                                                                                    gTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTAt                                                            <  1:2192363/148‑1 (MQ=255)
                                                                                     tCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATc                                                           >  1:2314483/1‑148 (MQ=255)
                                                                                      cTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCg                                                          >  1:852169/1‑148 (MQ=255)
                                                                                      cTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCg                                                          >  2:3174441/1‑148 (MQ=255)
                                                                                      cTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCg                                                          >  1:2582654/1‑148 (MQ=255)
                                                                                        gAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGt                                                        >  1:554774/1‑148 (MQ=255)
                                                                                        gAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGt                                                        >  1:554783/1‑148 (MQ=255)
                                                                                        gAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGt                                                        >  2:472570/1‑148 (MQ=255)
                                                                                        gAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGt                                                        >  2:2596398/1‑148 (MQ=255)
                                                                                         aGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTa                                                       >  1:832676/1‑148 (MQ=255)
                                                                                          gtgtCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTAt                                                      <  2:2973531/148‑1 (MQ=255)
                                                                                          gtgtCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTAt                                                      <  2:2774420/148‑1 (MQ=255)
                                                                                            gtCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATgg                                                    <  1:3677948/148‑1 (MQ=255)
                                                                                             tCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGa                                                   <  1:954356/148‑1 (MQ=255)
                                                                                             tCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGa                                                   <  2:3306247/148‑1 (MQ=255)
                                                                                             tCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGa                                                   >  2:3484180/1‑148 (MQ=255)
                                                                                               tttAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATc                                                 >  1:593978/1‑148 (MQ=255)
                                                                                                ttAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCa                                                <  2:2956668/148‑1 (MQ=255)
                                                                                                  aaCTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGGTTATCGGTATGGATCACt                                              >  2:342676/1‑148 (MQ=255)
                                                                                                   aCTCCGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACtt                                             <  2:2294680/148‑1 (MQ=255)
                                                                                                    cTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCCCttt                                            >  2:2032623/1‑148 (MQ=255)
                                                                                                       cgcgCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGcc                                         <  2:1230731/148‑1 (MQ=255)
                                                                                                           cATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTc                                     <  1:10797/148‑1 (MQ=255)
                                                                                                             tCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCg                                   >  2:1027811/1‑148 (MQ=255)
                                                                                                               tGCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGa                                 <  1:3197452/148‑1 (MQ=255)
                                                                                                                gCCGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGAt                                >  2:3501297/1‑148 (MQ=255)
                                                                                                                 ccGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATg                               >  2:2630605/1‑148 (MQ=255)
                                                                                                                  cGACCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGa                              <  2:2858014/148‑1 (MQ=255)
                                                                                                                    aCCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCTGTATGGATCACTTTGCCCGTCCGGATGACg                            >  1:3420242/1‑148 (MQ=255)
                                                                                                                    aCCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACg                            >  1:398815/1‑148 (MQ=255)
                                                                                                                    aCCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACg                            >  1:3420179/1‑148 (MQ=255)
                                                                                                                    aCCATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACg                            >  1:293820/1‑148 (MQ=255)
                                                                                                                     ccATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGa                           >  2:577644/1‑148 (MQ=255)
                                                                                                                      cATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAg                          >  2:2967818/1‑148 (MQ=255)
                                                                                                                      cATTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTGTGCCCGTCCGGATGACGAg                          >  2:2288156/1‑148 (MQ=255)
                                                                                                                       aTTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGc                         >  1:2195625/1‑148 (MQ=255)
                                                                                                                       aTTTTTGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGc                         <  1:226898/148‑1 (MQ=255)
                                                                                                                        tttttGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCt                        >  1:1034053/1‑148 (MQ=255)
                                                                                                                         ttttGCTGCTCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTg                       >  1:514213/1‑148 (MQ=255)
                                                                                                                               tgctCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTg                 >  2:2467386/1‑148 (MQ=255)
                                                                                                                                gctCAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTgg                >  1:879584/1‑148 (MQ=255)
                                                                                                                                   cAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGccc             >  1:1604622/1‑148 (MQ=255)
                                                                                                                                   cAGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGccc             >  1:3676922/1‑148 (MQ=255)
                                                                                                                                    aGCGCAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCa            >  2:2795165/1‑148 (MQ=255)
                                                                                                                                     gcgcAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAg           <  2:3020859/148‑1 (MQ=255)
                                                                                                                                     gcgcAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAg           >  1:1326923/1‑148 (MQ=255)
                                                                                                                                        cAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGt        >  1:2281242/1‑148 (MQ=255)
                                                                                                                                        cAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGt        >  1:1232483/1‑148 (MQ=255)
                                                                                                                                        cAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGt        >  1:3553094/1‑148 (MQ=255)
                                                                                                                                        cAAAATCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGt        >  1:2803534/1‑148 (MQ=255)
                                                                                                                                          aaaTCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGa      <  2:644879/148‑1 (MQ=255)
                                                                                                                                            aTCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAg    <  2:3018943/148‑1 (MQ=255)
                                                                                                                                            aTCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAg    >  1:1008167/1‑148 (MQ=255)
                                                                                                                                            aTCAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAg    >  1:2697572/1‑148 (MQ=255)
                                                                                                                                              cAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGc  >  1:2232852/1‑148 (MQ=255)
                                                                                                                                              cAATGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGc  >  1:1171221/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCGACCTGATTTACGGCCTGCCGAAACAGACGCCGGAGAGTTTCGCCTTTACCCTGAAACGTGTGGCGGAGCTGAACCCCGATCGTCTGAGTGTCTTTAACTACGCGCATCTGCCGACCATTTTTGCTGCTCAGCGCAAAATCAAAGATGCTGACCTGCCGAGTCCGCAGCAAAAACTCGATATCCTGCAGGAAACCATCGCCTTCCTGACGCAATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGC  >  CP009273/4046027‑4046316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑