BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,058,809	G→T	94.9%	I106I (ATC→ATA)	yihO ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,058,809	0	G	T	94.9%	598.8 / 19.8	219	I106I (ATC→ATA)	yihO	putative transporter
Reads supporting (aligned to +/- strand): ref base G (5/7); new base T (106/100); total (112/107)
Fisher's exact test for biased strand distribution p-value = 5.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

CGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACT  >  CP009273/4058664‑4058948

cgAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTAt                                                                                                                                           <  2:1319021/148‑1 (MQ=255)

  aGCTGCGCGCGCTCATTCTGGTTTTTGGTAATTGCCGGGAACATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATaa                                                                                                                                         <  2:1070135/148‑1 (MQ=255)

  aGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATaa                                                                                                                                         <  2:282763/148‑1 (MQ=255)

  aGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATaa                                                                                                                                         <  2:3429342/148‑1 (MQ=255)

    cTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAAc                                                                                                                                       <  2:2350465/148‑1 (MQ=255)

     tGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACt                                                                                                                                      <  1:1083736/148‑1 (MQ=255)

      gcgcgcgcTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTg                                                                                                                                     <  1:269014/148‑1 (MQ=255)

           cgcTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCg                                                                                                                                <  2:2890826/148‑1 (MQ=255)

           cgcTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCg                                                                                                                                <  2:1054035/148‑1 (MQ=255)

               cATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGc                                                                                                                            >  1:1505073/1‑148 (MQ=255)

                  tCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAAt                                                                                                                         >  1:177880/1‑148 (MQ=255)

                    gggTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTa                                                                                                                       <  2:1050384/148‑1 (MQ=255)

                     ggTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTaa                                                                                                                      <  1:1184746/148‑1 (MQ=255)

                       tttttGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAAcg                                                                                                                    >  2:558994/1‑148 (MQ=255)

                        ttttGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTAAAAACTGGAGCGTTGCAAATAAagc                                                                                                                   >  1:2865384/1‑148 (MQ=255)

                           tGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAAcgccgc                                                                                                                >  2:245529/1‑148 (MQ=255)

                            ggTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTTGCTATAAACTGGAGCGTTGCAATTAACgccgcc                                                                                                               <  2:3523057/148‑1 (MQ=255)

                             gTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGccgccg                                                                                                              >  1:1472758/1‑148 (MQ=255)

                             gTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGccgccg                                                                                                              >  1:435944/1‑148 (MQ=255)

                              tAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCgg                                                                                                             >  1:1074890/1‑148 (MQ=255)

                                 ttGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAAc                                                                                                          >  2:1948388/1‑148 (MQ=255)

                                    tcGGTATCATCGCACCATACGAGCAGTTCATCAGAGTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGc                                                                                                       <  1:1363599/147‑1 (MQ=255)

                                    ccGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGc                                                                                                       <  2:3048663/148‑1 (MQ=255)

                                    ccGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGc                                                                                                       <  1:2401196/148‑1 (MQ=255)

                                        gATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCa                                                                                                   >  2:863406/1‑148 (MQ=255)

                                        gATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCa                                                                                                   >  1:3016416/1‑148 (MQ=255)

                                         atcatcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTTGAGCGTTGCAATTAAAGCCGCCGGAACCGCTGCat                                                                                                  >  1:2171540/1‑148 (MQ=255)

                                              cGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaa                                                                                             <  1:1852298/148‑1 (MQ=255)

                                              cGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaa                                                                                             <  2:662981/148‑1 (MQ=255)

                                                 aCCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaaata                                                                                          <  1:3331265/148‑1 (MQ=255)

                                                  ccATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaaataa                                                                                         <  2:2672510/148‑1 (MQ=255)

                                                   cATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATataaaataaa                                                                                        <  1:15164/148‑1 (MQ=255)

                                                     tACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAgg                                                                                      >  1:2208106/1‑148 (MQ=255)

                                                      aCGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAggg                                                                                     <  2:1262364/148‑1 (MQ=255)

                                                      aCGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAggg                                                                                     <  2:3167148/148‑1 (MQ=255)

                                                         aGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACg                                                                                  >  2:1939220/1‑148 (MQ=255)

                                                            aGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGaaa                                                                               <  2:1070656/148‑1 (MQ=255)

                                                             gTTCATCAGACTTTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAAt                                                                              <  1:513965/148‑1 (MQ=255)

                                                             gTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAAt                                                                              <  1:3690492/148‑1 (MQ=255)

                                                              ttCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAAtt                                                                             >  1:60534/1‑148 (MQ=255)

                                                                catcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTg                                                                           >  2:1454295/1‑148 (MQ=255)

                                                                  tcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGcc                                                                         <  1:3416487/148‑1 (MQ=255)

                                                                  tcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGcc                                                                         <  1:3416661/148‑1 (MQ=255)

                                                                  tcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGcc                                                                         >  2:1690061/1‑148 (MQ=255)

                                                                  tcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGcc                                                                         <  1:468514/148‑1 (MQ=255)

                                                                   caGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCt                                                                        <  2:2301063/148‑1 (MQ=255)

                                                                   caGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCt                                                                        <  2:3661349/148‑1 (MQ=255)

                                                                    aGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCtt                                                                       <  2:442733/148‑1 (MQ=255)

                                                                     gACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCttt                                                                      >  2:721899/1‑148 (MQ=255)

                                                                      aCTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTa                                                                     >  2:818164/1‑148 (MQ=255)

                                                                        tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAgg                                                                   <  2:2469337/148‑1 (MQ=255)

                                                                         gTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGc                                                                  >  2:159775/1‑148 (MQ=255)

                                                                            tGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCa                                                               <  2:848419/148‑1 (MQ=255)

                                                                              aaaGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAat                                                             <  1:587167/148‑1 (MQ=255)

                                                                                aGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAatat                                                           >  2:1919203/1‑148 (MQ=255)

                                                                                aGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAatat                                                           >  1:746002/1‑148 (MQ=255)

                                                                                 ttCCAAACATAATAAACAGCGCGGTGGCAATGGTCTTTTTAACCGGCAATCAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTACCGCCGCCGTAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAtt                                                          <  1:2084875/147‑1 (MQ=255)

                                                                                 gTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACATAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAAGCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAtt                                                          <  2:50040/148‑1 (MQ=255)

                                                                                  tCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTTCCTTTAGGCCCAATAttt                                                         >  1:2058373/1‑148 (MQ=255)

                                                                                    cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAttttt                                                       <  2:2771150/148‑1 (MQ=255)

                                                                                    cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAttttt                                                       >  2:1582182/1‑148 (MQ=255)

                                                                                    cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATAttttt                                                       <  2:3690485/148‑1 (MQ=255)

                                                                                       aCATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAAATGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTAcg                                                    >  1:3786039/1‑148 (MQ=255)

                                                                                          tcatAAACAGCGCGGTGGCAATCGTCGTGTTAACCGGCAAAAAAAAGGTGGTGGCTATAAACTGGATCGTTGCAATTAAAGCCGCCGGAAACGCGGCATATAAAATAAAGGGACGAAATTTGGCTTTAGGCCCAATATTTTTCCGcga                                                 >  1:2749695/1‑148 (MQ=255)

                                                                                           catAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgag                                                >  2:2082523/1‑148 (MQ=255)

                                                                                            atAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgagt                                               <  2:3768441/148‑1 (MQ=255)

                                                                                             tAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCGTTAGGCCCAAAATTTTTCCGcgagtc                                              >  1:3306711/1‑148 (MQ=255)

                                                                                              aaaCCGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgagtcg                                             <  1:3055290/148‑1 (MQ=255)

                                                                                               aaCAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgagtcga                                            >  2:2452085/1‑148 (MQ=255)

                                                                                               aaCAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgagtcga                                            >  2:951978/1‑148 (MQ=255)

                                                                                                aCAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGcgagtcgag                                           >  2:3700766/1‑148 (MQ=255)

                                                                                                     gcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTaaaa                                      <  1:2331848/148‑1 (MQ=255)

                                                                                                      cgGTGGCAATCGTAGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATGTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTaaaaa                                     <  1:3270389/148‑1 (MQ=255)

                                                                                                        gTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATc                                   <  2:1064119/148‑1 (MQ=255)

                                                                                                          ggCAATCGTCGTTTTAACCGGCAAAAAAAAGGTGGTGGATATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCg                                 <  1:1646093/148‑1 (MQ=255)

                                                                                                             aaTCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTg                              <  2:60534/148‑1 (MQ=255)

                                                                                                               tcgtcgTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAg                            <  1:245529/148‑1 (MQ=255)

                                                                                                               tcgtcgTTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTACGCGAGTCGAGTAAAAATCCGGTGAg                            >  1:2774533/1‑148 (MQ=255)

                                                                                                                cgtcgtTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGc                           <  2:2544975/148‑1 (MQ=255)

                                                                                                                    gtTTTAACCGGCAAACACAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAAGCGCTGCATATAACATAAAGGGACGATATTTGCCTTTAGTCCCAATATTTTTCCGCGAGTAGAGTAAAAATCCGGTGAGCattt                       <  2:2247892/148‑3 (MQ=255)

                                                                                                                    gtTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCatat                       <  2:1811256/148‑1 (MQ=255)

                                                                                                                    gtTTTAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCatat                       <  1:258256/148‑1 (MQ=255)

                                                                                                                      tttAACCTGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCg                     <  2:3034897/148‑1 (MQ=255)

                                                                                                                      tttAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCg                     >  1:2493107/1‑148 (MQ=255)

                                                                                                                      tttAACCGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCg                     >  1:3405256/1‑148 (MQ=255)

                                                                                                                      tttAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCg                     >  1:195014/1‑148 (MQ=255)

                                                                                                                           ccGGCAAACAAAAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTaaa                >  1:1039777/1‑148 (MQ=255)

                                                                                                                               caaacaatAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAcgcg            <  2:2242893/148‑1 (MQ=255)

                                                                                                                                 aacaaaAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGt          >  1:547104/1‑148 (MQ=255)

                                                                                                                                 aacaaaAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGt          >  1:3767050/1‑148 (MQ=255)

                                                                                                                                  acaaaAGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTa         >  1:2151473/1‑148 (MQ=255)

                                                                                                                                    aaaaGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaaa       >  1:893110/1‑148 (MQ=255)

                                                                                                                                     aaaGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaaaa      >  1:972011/1‑148 (MQ=255)

                                                                                                                                      aaGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaaaaa     >  1:3124321/1‑148 (MQ=255)

                                                                                                                                      aaGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaaaaa     >  1:3079312/1‑148 (MQ=255)

                                                                                                                                      aaGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaaaaa     <  1:2787693/148‑1 (MQ=255)

                                                                                                                                       aGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaaaaaa    <  1:3638021/148‑1 (MQ=255)

                                                                                                                                       aGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaaaaaa    >  2:127583/1‑148 (MQ=255)

                                                                                                                                       aGGTGGTGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTaaaaaa    >  2:127729/1‑148 (MQ=255)

                                                                                                                                        ggtggtGGCTATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAAc   >  2:3347816/1‑148 (MQ=255)

CGAGCTGCGCGCGCTCATTCGGGTTTTTGGTAATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACT  >  CP009273/4058664‑4058948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`