BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	317,205	G→A	100%	intergenic (+413/‑114)	rclR → / → ykgE	reactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein; putative electron transport chain YkgEFG component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	317,205	0	G	A	98.2%	981.6 / 6.5	333	intergenic (+413/‑114)	rclR/ykgE	reactive chlorine species (RCS)‑specific activator of the rcl genes/cysteine‑rich LutA family protein; putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand): ref base G (4/2); new base A (150/171); total (154/179)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 334 total aligned reads displayed.

AGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTA  >  CP009273/317061‑317346

     tGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTa                                                                                                                                       <  2:2231589/148‑1 (MQ=255)

       tAGCCATTTTTAAATGGATTTCTTATTCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATg                                                                                                                                     <  2:1960177/148‑1 (MQ=255)

         gCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGtt                                                                                                                                   >  2:1012972/1‑148 (MQ=255)

             tttttAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTCTGTTGCTa                                                                                                                               >  1:2269103/1‑148 (MQ=255)

             tttttAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTa                                                                                                                               >  1:2466430/1‑148 (MQ=255)

                 tAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCa                                                                                                                           >  1:118557/1‑148 (MQ=255)

                   aaTGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCact                                                                                                                         >  2:2862676/1‑146 (MQ=255)

                    aTGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAAtt                                                                                                                        >  2:1093538/1‑148 (MQ=255)

                      ggATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTaa                                                                                                                      >  2:261317/1‑148 (MQ=255)

                       gATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTaaa                                                                                                                     <  1:3451218/148‑1 (MQ=255)

                        aTTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTaaaa                                                                                                                    >  2:682567/1‑148 (MQ=255)

                         tttCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTaaaaa                                                                                                                   >  1:955897/1‑148 (MQ=255)

                          ttCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCTATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTaaaaaa                                                                                                                  >  1:442471/1‑148 (MQ=255)

                          ttCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTaaaaaa                                                                                                                  <  1:1477675/148‑1 (MQ=255)

                            cTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGa                                                                                                                <  1:92050/148‑1 (MQ=255)

                             ttATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGaa                                                                                                               <  2:3048696/148‑1 (MQ=255)

                             ttATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGaa                                                                                                               >  1:1251521/1‑148 (MQ=255)

                             ttATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGaa                                                                                                               <  2:1143830/148‑1 (MQ=255)

                               aTGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAATGATTATCATTTTTCATACAAATAAAAGGTGGTATGTTGCTAGCCAATTAAAAAAGAACg                                                                                                             <  1:3272970/148‑1 (MQ=255)

                               aTGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACg                                                                                                             <  2:1220683/148‑1 (MQ=255)

                                tGCCAAAAATTATCGTCGATTTCCCCATAAAATGTGAGCGATGCATAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAATATTATCATTTGTAATACAAATAAAGGGTGTTATGTTGCTAGCCAATTAAAAAAGAACGc                                                                                                            <  1:2909977/148‑1 (MQ=255)

                                tGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGc                                                                                                            >  2:479089/1‑148 (MQ=255)

                                    aaaatTGATCGTCTTTTTCCCCATAAAATGTGAGCGATGCCGAAATAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAAGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCata                                                                                                        <  1:3108241/148‑1 (MQ=255)

                                        aTGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAATAAATAAAATTATTTATTGCATTTGACGTTTGGATTAAATATTATCATTTTTAATACAAATAACGGGTGGTACGTTGCTAGCCAATTAAAAAAGAACGCCAtattta                                                                                                    <  1:3558863/148‑1 (MQ=255)

                                         tGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGAATTTGACGTTTGGATGAAATATTATCATTTGTCATACAAATAAAAGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCAtatttat                                                                                                   <  1:2989484/148‑1 (MQ=255)

                                           aTCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtg                                                                                                 <  2:3585725/148‑1 (MQ=255)

                                             cgtcgATTTCCCCAAAAAAAGTGAGCGATGCCGAAATAAAAAAAAATAGTTATTGCATTTGACGTTTTGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAAATAAAAAAGAAAGCCAAATTTATtgat                                                                                               >  1:1666040/1‑148 (MQ=255)

                                              gtcgATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATtgatg                                                                                              <  2:1427446/148‑1 (MQ=255)

                                                cgATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTgatgat                                                                                            <  2:1046522/148‑1 (MQ=255)

                                                  aTTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGAtgattg                                                                                          >  1:3760753/1‑148 (MQ=255)

                                                   tttCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGAtgattga                                                                                         <  1:1903901/148‑1 (MQ=255)

                                                    ttCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGAtgattgat                                                                                        <  1:1777415/148‑1 (MQ=255)

                                                    ttCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGAtgattgat                                                                                        >  2:3525347/1‑148 (MQ=255)

                                                     tCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAAGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATc                                                                                       <  1:3376959/148‑1 (MQ=255)

                                                     gccccATAAATTGTGAGCGATGCCTAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAAGGTGGTATGTTGCTCGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATc                                                                                       <  1:1056914/147‑1 (MQ=255)

                                                      ccccATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATcc                                                                                      <  2:1822365/148‑1 (MQ=255)

                                                         cATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATccccc                                                                                   >  1:508852/1‑148 (MQ=255)

                                                           tAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCgg                                                                                 >  2:1849504/1‑148 (MQ=255)

                                                            aaaaTGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGt                                                                                >  1:2841473/1‑148 (MQ=255)

                                                            aaaaTGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGt                                                                                <  1:3692902/148‑1 (MQ=255)

                                                               aTGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTggg                                                                             <  1:2092331/148‑1 (MQ=255)

                                                                tgtgAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTgggg                                                                            >  1:1005533/1‑148 (MQ=255)

                                                                  tgAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAAGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTgggggg                                                                          <  2:3287354/148‑1 (MQ=255)

                                                                   gAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAAGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTggggggg                                                                         <  1:1507622/148‑1 (MQ=255)

                                                                    aGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATAGTTATTGATGATTGATCACCCGGTGGGGGGGa                                                                        >  1:3666754/1‑148 (MQ=255)

                                                                      cGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGaaa                                                                      <  2:2087469/148‑1 (MQ=255)

                                                                      cGATGCCGAAACAAATAAAATTAGTTAGGGCATTTGACGTTGGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGGTGCTAGCCAAGTAAAAAAGAACGCCATATTGATTGATGATTGATCCCCCGGTGGGGGGGaaa                                                                      <  2:2087711/148‑1 (MQ=255)

                                                                       gATGCCTAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTAACATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAAc                                                                     <  2:3366611/148‑1 (MQ=255)

                                                                        aTGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACa                                                                    <  1:2182629/148‑1 (MQ=255)

                                                                           ccGAAACAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAACGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCg                                                                 <  1:2700376/148‑1 (MQ=255)

                                                                            cGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCgg                                                                >  2:363038/1‑148 (MQ=255)

                                                                                   aaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAAGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATaaa                                                         >  1:2067834/1‑148 (MQ=255)

                                                                                    aTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAAt                                                        >  2:605677/1‑148 (MQ=255)

                                                                                    aTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAAt                                                        >  1:2085737/1‑148 (MQ=255)

                                                                                     tAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATg                                                       >  1:2747000/1‑148 (MQ=255)

                                                                                      aaaaTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGAtgattg                                                                                          >  2:3762370/1‑112 (MQ=255)

                                                                                      aaaaTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAAGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATgg                                                      >  2:3223/1‑148 (MQ=255)

                                                                                        aaTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGc                                                    >  1:140509/1‑148 (MQ=255)

                                                                                        aaTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGc                                                    >  1:3186802/1‑148 (MQ=255)

                                                                                        aaTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGc                                                    >  2:827120/1‑148 (MQ=255)

                                                                                         aTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCACCCGGTGGGGGGGAAACATCGGGCATAAATGGTCa                                                   >  2:3127082/1‑148 (MQ=255)

                                                                                         aTTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAAGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCa                                                   <  1:1359691/148‑1 (MQ=255)

                                                                                          ttAGTTATTGCATTTGACGTTTGTATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCAt                                                  <  1:2376224/148‑1 (MQ=255)

                                                                                            aGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAAGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGa                                                <  2:1255883/148‑1 (MQ=255)

                                                                                             gTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGaa                                               <  2:334012/148‑1 (MQ=255)

                                                                                             gTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGaa                                               <  2:2938276/148‑1 (MQ=255)

                                                                                             gTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGaa                                               <  2:1833473/148‑1 (MQ=255)

                                                                                                aTTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTa                                            <  2:3199197/148‑1 (MQ=255)

                                                                                                    cATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAAGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATgg                                        <  2:1561760/148‑1 (MQ=255)

                                                                                                     aTTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAAGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGa                                       <  2:280840/148‑1 (MQ=255)

                                                                                                      tttGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAg                                      >  2:1439757/1‑148 (MQ=255)

                                                                                                       ttGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGt                                     >  1:2604235/1‑148 (MQ=255)

                                                                                                       ttGACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAAGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGt                                     >  2:2117519/1‑148 (MQ=255)

                                                                                                        tGACGTTTTGATGAAAGATTATCATTTGTCATACAAATAAAAGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTa                                    <  2:2490328/148‑1 (MQ=255)

                                                                                                         gACGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTAt                                   <  2:17886/148‑1 (MQ=255)

                                                                                                          aCGTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTAtt                                  >  1:1541357/1‑148 (MQ=255)

                                                                                                            gTTTGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAg                                <  1:1093538/148‑1 (MQ=255)

                                                                                                            gTTTGGATGAAAGATTATCATTTGTAATACAAATAAAAGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAg                                <  1:1987521/148‑1 (MQ=255)

                                                                                                               tGGATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCAAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTtg                             <  1:669958/148‑1 (MQ=255)

                                                                                                                 gATGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTtgtg                           >  1:3151739/1‑148 (MQ=255)

                                                                                                                  aTGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGa                          >  2:1164264/1‑148 (MQ=255)

                                                                                                                   tGAAAGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGaa                         >  1:3785222/1‑148 (MQ=255)

                                                                                                                   tGAAAGATTATCATTTGTCATACAAATAAAAGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGaa                         >  2:3786693/1‑148 (MQ=255)

                                                                                                                       aGATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTc                     <  2:1882870/148‑1 (MQ=255)

                                                                                                                        gATTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTA‑TAGTTGTGTAtttaca                   >  2:2377310/1‑143 (MQ=255)

                                                                                                                         aTTATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCaa                   <  1:599846/148‑1 (MQ=255)

                                                                                                                          ttATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAt                  <  2:1297017/148‑1 (MQ=255)

                                                                                                                          ttATCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAt                  >  1:1225139/1‑148 (MQ=255)

                                                                                                                            aTCATTTGTCATACATATAATGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCACCCGGTGGGGGGGAAACATCGGGGATAAATGGGTATGAAGTAATGGATTATTAGTTGTGAATGTCAAttt                <  2:134919/148‑1 (MQ=255)

                                                                                                                             tCATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTc               <  1:374724/148‑1 (MQ=255)

                                                                                                                              cATTTGTCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCt              >  2:119312/1‑148 (MQ=255)

                                                                                                                                gttGTAATACAAATAAAGTGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCttt            <  2:743518/147‑1 (MQ=255)

                                                                                                                                    tCATACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCa        >  1:2539983/1‑148 (MQ=255)

                                                                                                                                      aTACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAcc      <  1:3353125/148‑1 (MQ=255)

                                                                                                                                      aTACAAATAAAAGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAcc      >  1:258387/1‑148 (MQ=255)

                                                                                                                                       tACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCt     >  2:95393/1‑148 (MQ=255)

                                                                                                                                       tACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCt     >  1:3027222/1‑148 (MQ=255)

                                                                                                                                       tACAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCt     >  2:1914813/1‑148 (MQ=255)

                                                                                                                                       tACAAATAAAAGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCt     >  1:1350634/1‑148 (MQ=255)

                                                                                                                                         cAAATAAAGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGt   <  1:216008/148‑1 (MQ=255)

AGGGATGTAGCCATTTTTAAATGGATTTCTTATGCCAAAAATGATCGTCGATTTCCCCATAAAATGTGAGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCCCGGTGGGGGGGAAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTA  >  CP009273/317061‑317346

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`