Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,173,145 A→T 100% Q658L (CAG→CTG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,173,1450AT100.0% 792.8 / ‑6.7 239Q658L (CAG→CTG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (131/107);  total (132/107)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 245 total aligned reads displayed.

GAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACG  >  CP009273/4173000‑4173289
                                                                                                                                                 |                                                                                                                                                
gaagaaGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCctgc                                                                                                                                                <  2:346078/148‑1 (MQ=255)
 aagaagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCctgca                                                                                                                                               <  2:2102879/148‑1 (MQ=255)
  agaagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCctgcag                                                                                                                                              >  2:2837626/1‑148 (MQ=255)
  agaagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCctgcag                                                                                                                                              >  2:3695521/1‑148 (MQ=255)
   gaagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAgg                                                                                                                                             >  2:1069079/1‑148 (MQ=255)
    aagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAggt                                                                                                                                            >  1:3548240/1‑148 (MQ=255)
    aagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAggt                                                                                                                                            >  2:1120323/1‑148 (MQ=255)
    aagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAggt                                                                                                                                            >  2:3026912/1‑148 (MQ=255)
      ggCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAggtgg                                                                                                                                          >  2:850455/1‑148 (MQ=255)
      ggCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAggtgg                                                                                                                                          >  2:825921/1‑148 (MQ=255)
       gCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAggtggt                                                                                                                                         <  1:1631029/148‑1 (MQ=255)
       gCAACTACGTTATCGCCCAGGCGAACTCCAACCTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAggtggt                                                                                                                                         <  2:2516813/148‑1 (MQ=255)
        cAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTa                                                                                                                                        <  2:397218/148‑1 (MQ=255)
          aCTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATc                                                                                                                                      >  1:1236312/1‑148 (MQ=255)
           cTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATcc                                                                                                                                     >  1:1714604/1‑148 (MQ=255)
             aCGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGt                                                                                                                                   >  2:501175/1‑148 (MQ=255)
              cGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTc                                                                                                                                  >  1:2527162/1‑148 (MQ=255)
               gTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCg                                                                                                                                 <  1:3820518/148‑1 (MQ=255)
                    cGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCg                                                                                                                            >  2:1269460/1‑148 (MQ=255)
                       ccAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTcc                                                                                                                         <  1:1002026/148‑1 (MQ=255)
                        cAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTccc                                                                                                                        <  1:2411129/148‑1 (MQ=255)
                        cAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTccc                                                                                                                        <  1:3082361/148‑1 (MQ=255)
                           gCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGa                                                                                                                     >  2:503580/1‑148 (MQ=255)
                           gCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGa                                                                                                                     >  2:177016/1‑148 (MQ=255)
                                cTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATccct                                                                                                                >  1:2758370/1‑147 (MQ=255)
                                 tCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATccctt                                                                                                               >  1:751423/1‑146 (MQ=255)
                                     aCTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCt                                                                                                           <  1:1688725/148‑1 (MQ=255)
                                      cTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTg                                                                                                          >  2:134545/1‑148 (MQ=255)
                                        tGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGa                                                                                                        >  2:3213067/1‑148 (MQ=255)
                                           aTGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAaca                                                                                                     >  2:1900982/1‑148 (MQ=255)
                                           aTGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAaca                                                                                                     >  2:2593651/1‑148 (MQ=255)
                                           aTGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAaca                                                                                                     >  2:2914729/1‑148 (MQ=255)
                                                gaagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATg                                                                                                <  1:1431597/148‑1 (MQ=255)
                                                 aagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGa                                                                                               >  1:93974/1‑148 (MQ=255)
                                                 aagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGa                                                                                               >  1:3807582/1‑148 (MQ=255)
                                                       aCTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCaa                                                                                         <  1:3356170/148‑1 (MQ=255)
                                                          tCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCg                                                                                      <  2:1794842/148‑1 (MQ=255)
                                                              agaagaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCa                                                                                  >  1:770369/1‑148 (MQ=255)
                                                               gaagaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCAt                                                                                 >  2:3057220/1‑148 (MQ=255)
                                                               gaagaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCAt                                                                                 <  2:1545363/148‑1 (MQ=255)
                                                               gaagaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCAt                                                                                 >  2:631257/1‑148 (MQ=255)
                                                                aagaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCAtt                                                                                >  1:3336437/1‑148 (MQ=255)
                                                                aagaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCAtt                                                                                >  2:76202/1‑148 (MQ=255)
                                                                  gaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGa                                                                              <  2:2936946/148‑1 (MQ=255)
                                                                  gaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGa                                                                              <  2:1027621/148‑1 (MQ=255)
                                                                  gaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGa                                                                              <  2:3454057/148‑1 (MQ=255)
                                                                  gaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGa                                                                              >  2:626717/1‑148 (MQ=255)
                                                                   aCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGAt                                                                             >  1:2799246/1‑148 (MQ=255)
                                                                      tGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATggg                                                                          >  2:3237182/1‑148 (MQ=255)
                                                                         tAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGc                                                                       <  2:2452072/148‑1 (MQ=255)
                                                                         tAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGc                                                                       >  1:542815/1‑148 (MQ=255)
                                                                           aCTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGa                                                                     <  1:260770/148‑1 (MQ=255)
                                                                           aCTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTACGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGa                                                                     >  1:2088519/1‑148 (MQ=255)
                                                                              tGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACa                                                                  <  2:3548240/148‑1 (MQ=255)
                                                                                ccGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATg                                                                <  2:1189464/148‑1 (MQ=255)
                                                                                ccGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATg                                                                <  1:1979610/148‑1 (MQ=255)
                                                                                  gTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCa                                                              <  1:2502304/148‑1 (MQ=255)
                                                                                   tAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCaa                                                             >  2:1818178/1‑148 (MQ=255)
                                                                                     gCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACg                                                           <  1:1295925/148‑1 (MQ=255)
                                                                                       aaaGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTc                                                         <  2:234250/148‑1 (MQ=255)
                                                                                       aaaGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTc                                                         <  2:2259344/148‑1 (MQ=255)
                                                                                       aaaGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTc                                                         <  1:850730/148‑1 (MQ=255)
                                                                                         aGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAg                                                       >  1:1655802/1‑148 (MQ=255)
                                                                                         aGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAg                                                       >  2:1649472/1‑148 (MQ=255)
                                                                                          ggCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAgg                                                      <  2:3808473/148‑1 (MQ=255)
                                                                                          ggCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAgg                                                      <  1:174612/148‑1 (MQ=255)
                                                                                               aTCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACAATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGtt                                                 <  2:1484838/148‑1 (MQ=255)
                                                                                                tCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTc                                                >  2:1839959/1‑148 (MQ=255)
                                                                                                tCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTc                                                >  2:2819481/1‑148 (MQ=255)
                                                                                                    gCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGACAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGAc                                            >  1:855902/1‑148 (MQ=255)
                                                                                                     cTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGAct                                           <  1:2671356/148‑1 (MQ=255)
                                                                                                       tGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGActct                                         >  1:182376/1‑148 (MQ=255)
                                                                                                          tCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcg                                      >  1:3699335/1‑148 (MQ=255)
                                                                                                            aGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcgcg                                    <  2:401485/148‑1 (MQ=255)
                                                                                                            aGCCGCGACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcgcg                                    <  2:2196162/148‑1 (MQ=255)
                                                                                                                 cgACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGAt                               <  2:1560055/148‑1 (MQ=255)
                                                                                                                 cgACCAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGAt                               <  2:427419/148‑1 (MQ=255)
                                                                                                                    ccAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAg                            <  1:3757305/148‑1 (MQ=255)
                                                                                                                     cAGGTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGc                           >  2:1333706/1‑148 (MQ=255)
                                                                                                                       ggTTGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCg                         >  1:1018083/1‑148 (MQ=255)
                                                                                                                         ttGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCt                       <  1:1120323/148‑1 (MQ=255)
                                                                                                                         ttGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCt                       >  1:618732/1‑148 (MQ=255)
                                                                                                                         ttGACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCt                       <  1:3026912/148‑1 (MQ=255)
                                                                                                                           gACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtgg                     >  2:804466/1‑148 (MQ=255)
                                                                                                                           gACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtgg                     <  1:1921884/148‑1 (MQ=255)
                                                                                                                           gACTACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtgg                     <  1:356059/148‑1 (MQ=255)
                                                                                                                              tACATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttg                  <  1:2157164/148‑1 (MQ=255)
                                                                                                                               aCATGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttgg                 <  2:2758370/148‑1 (MQ=255)
                                                                                                                                  tGGACGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTAc              >  2:3604016/1‑148 (MQ=255)
                                                                                                                                     aCGTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGNGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTgg           >  1:1937181/1‑148 (MQ=255)
                                                                                                                                       gTATCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGAAGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGTCTCTGCGCGCTGATAAGCCGCTGGTTTGTACTTGTa         <  1:2236607/148‑1 (MQ=255)
                                                                                                                                          tCCACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATgg      <  1:134545/148‑1 (MQ=255)
                                                                                                                                           ccACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGa     <  1:503580/148‑1 (MQ=255)
                                                                                                                                           ccACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGa     <  2:1420700/148‑1 (MQ=255)
                                                                                                                                           ccACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGa     >  1:2278673/1‑148 (MQ=255)
                                                                                                                                           ccACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGa     <  1:2837626/148‑1 (MQ=255)
                                                                                                                                            cACCCTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCGGATAAGCCGCTGGTTGGTACTGGTATGGaa    <  2:1820818/148‑1 (MQ=255)
                                                                                                                                              cccTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACg  >  2:1962288/1‑148 (MQ=255)
                                                                                                                                              cccTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACg  <  2:2708351/148‑1 (MQ=255)
                                                                                                                                              cccTGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCTTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACg  <  2:3175510/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACG  >  CP009273/4173000‑4173289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑