BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,212,209	G→T	5.7%	V69V (GTC→GTA)	arpA ←	ankyrin repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,212,209	0	G	T	5.7%	1030.7 / 6.9	306	V69V (GTC→GTA)	arpA	ankyrin repeat protein
Reads supporting (aligned to +/- strand): ref base G (141/138); new base T (25/2); total (166/140)
Fisher's exact test for biased strand distribution p-value = 1.02e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 308 total aligned reads displayed.

AGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCACTCAGGGTTTGGTGTTCATTT  >  CP009273/4212063‑4212356

agagTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGa                                                                                                                                                    >  2:2613077/1‑148 (MQ=255)

    tgtCTGAGCGTTGATGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTTTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACaaa                                                                                                                                                <  1:2289164/148‑1 (MQ=255)

      tCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAAt                                                                                                                                              <  2:1697278/148‑1 (MQ=255)

       cTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTTTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTATTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGAAAAAAta                                                                                                                                             >  2:2833657/1‑147 (MQ=255)

       cTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATc                                                                                                                                             <  1:3492326/148‑1 (MQ=255)

       cTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATAGTAATTTATTTCTTATAAGCTTGTAAGACGGACGGTGAAAATTTAATTACAAAATc                                                                                                                                             >  1:3052985/1‑148 (MQ=255)

          aGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCtt                                                                                                                                          <  2:3411479/148‑1 (MQ=255)

          aGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCtt                                                                                                                                          <  1:1530837/148‑1 (MQ=255)

           gCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCttg                                                                                                                                         <  1:3049959/148‑1 (MQ=255)

               tGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGc                                                                                                                                     <  2:95602/148‑1 (MQ=255)

               tGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGc                                                                                                                                     <  2:2420835/148‑1 (MQ=255)

                 aGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTa                                                                                                                                   >  2:1454566/1‑148 (MQ=255)

                 aGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATACTTTTTGCTa                                                                                                                                   >  2:2334945/1‑148 (MQ=255)

                   gTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATACTTGTTGCTaaa                                                                                                                                 >  1:3104260/1‑148 (MQ=255)

                      aaaTTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGat                                                                                                                              <  1:1398252/148‑1 (MQ=255)

                        aTTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCATCTTGTAAGACGGACGTGGAAAAATTAATGACAAAATACTTGTTGCTAAatatat                                                                                                                            >  1:1211978/1‑148 (MQ=255)

                        aTTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGGAATTTCTTTCTTATCAGCTTTTAAGACGGACGTGGAAAATTTAATTACAAAATACATGTTGGTAAAGatat                                                                                                                            >  1:1155069/1‑148 (MQ=255)

                          ttCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATga                                                                                                                          <  1:62020/148‑1 (MQ=255)

                                tAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCa                                                                                                                    >  1:2117672/1‑148 (MQ=255)

                                    tATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTTTTAATTATAGTAATTTATTTCTTATAAGCTTGTAATAAGGACGTGGTAAAATTAATGACAAAAACATTGTTGCTAAATATATGACGAACAtttt                                                                                                                >  1:811707/1‑148 (MQ=255)

                                    tATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCAtttt                                                                                                                <  2:2549363/148‑1 (MQ=255)

                                    tATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCAtttt                                                                                                                >  2:929071/1‑148 (MQ=255)

                                     aTTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTATTTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGTAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTg                                                                                                               >  1:963945/1‑148 (MQ=255)

                                      tttttCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGt                                                                                                              <  1:1747096/148‑1 (MQ=255)

                                      tttttCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGt                                                                                                              <  2:839614/148‑1 (MQ=255)

                                             aaTATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCGTGTTGCTAAAGATATGACGACCATTTTGTCTACAGt                                                                                                       >  2:1749072/1‑148 (MQ=255)

                                              atatTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTGTTTCTTATCAGCTTGTAATAAGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCAATTTTTCTACAGtt                                                                                                      >  1:524865/1‑148 (MQ=255)

                                              atatTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGtt                                                                                                      <  2:1522783/148‑1 (MQ=255)

                                              atatTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGtt                                                                                                      <  1:1284860/148‑1 (MQ=255)

                                               tatTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTtc                                                                                                     <  2:1557482/148‑1 (MQ=255)

                                                atTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTtct                                                                                                    <  1:369647/148‑1 (MQ=255)

                                                 ttGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTtctc                                                                                                   <  2:1333762/148‑1 (MQ=255)

                                                 ttGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTAGTTTTTATTAATTATCGTAATTTCTTTCTTATAAGCTTTTAAGTCGGACGTGGAAAATTTAATGAAAAAATCCTTGTTGCTAAAGATATTACCAACAATTTTTCTAAAGTtctc                                                                                                   >  2:2563597/1‑148 (MQ=255)

                                                       cAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTTATTTATTTTTTATAATCATTTAAAACGGACGGGGAAAATTTAATTAAAAAATAATTTTTTGTAAATATATTAAGACCCTTTTTTCTTCAGTTATATGAGaa                                                                                             >  1:3238774/1‑148 (MQ=255)

                                                         aTGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGc                                                                                           <  2:3793911/148‑1 (MQ=255)

                                                         aTGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGc                                                                                           <  1:442223/148‑1 (MQ=255)

                                                          tGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTATCTGAGAAGCt                                                                                          >  2:1142034/1‑148 (MQ=255)

                                                           ggTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCtt                                                                                         <  2:2376787/148‑1 (MQ=255)

                                                             tttGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTATTTATTATAAGCTTGTAAGAAGGACGTGGAAAATTTAATTAAAAAATACATGTTTCTAAAGATATTAAGACCATTTTTTCTACAGTTATATGAGAAGCtttt                                                                                       >  1:1499445/1‑148 (MQ=255)

                                                               tGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTa                                                                                     <  1:1956658/148‑1 (MQ=255)

                                                                  gTAAGTGTCGTGTTTTCGTTTTTATTAATTATAGTAAATTATTTATTATAAGCTTTTTAGAAGGACGTGGAAAAATTAATTACAAAAAACATGTTGCTAAATATATTAAGCCCAATTTTTATAAACTTATCTGAGAATCTTTTTAATa                                                                                  >  1:497820/1‑148 (MQ=255)

                                                                   tAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATag                                                                                 >  2:80575/1‑148 (MQ=255)

                                                                     aGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATTACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATagag                                                                               >  2:3357206/1‑148 (MQ=255)

                                                                     aGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATagag                                                                               <  2:882490/148‑1 (MQ=255)

                                                                         tCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAATACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAATATATTACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGt                                                                           >  2:676902/1‑148 (MQ=255)

                                                                               tttCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAATACGGACGTGGAAAATTTAATTACAAAATCCTTGTTGCTAAAGATATTACGACCATTTTGTATACAGTTCTCTGAGAAGCTTTTTAATATAGGCGTCGCCAg                                                                     >  1:2400201/1‑148 (MQ=255)

                                                                                ttCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGc                                                                    >  1:765417/1‑148 (MQ=255)

                                                                                      tttATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTg                                                              <  1:2546114/148‑1 (MQ=255)

                                                                                           taattaTCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGa                                                         >  1:2719668/1‑148 (MQ=255)

                                                                                           taattaTCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAAACCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATATAGGCGTCGCCAGCTCCTTGCCAGa                                                         >  1:1015092/1‑148 (MQ=255)

                                                                                              ttaTCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGacaa                                                      >  2:1859070/1‑148 (MQ=255)

                                                                                              ttaTCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCAAAAGATATGAAGACCATGTTGTCTACAGTTCTCTGAGAAGATTTTTAATAGATGCGTCGCCACCTCCTTGCCAGaaaa                                                      >  2:2189495/1‑148 (MQ=255)

                                                                                               taTCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAAt                                                     <  2:589948/148‑1 (MQ=255)

                                                                                                   gTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTAt                                                 <  2:2714580/148‑1 (MQ=255)

                                                                                                    tAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATc                                                >  2:3707457/1‑148 (MQ=255)

                                                                                                    tAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATc                                                >  2:629558/1‑148 (MQ=255)

                                                                                                    tAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATc                                                <  1:2613077/148‑1 (MQ=255)

                                                                                                     aaTTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTtct                                                                                                    >  2:1090895/1‑95 (MQ=255)

                                                                                                       tttctttcTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTc                                             >  2:3087818/1‑148 (MQ=255)

                                                                                                        ttctttctTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCg                                            >  1:1261600/1‑148 (MQ=255)

                                                                                                        ttctttctTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCg                                            >  1:3165334/1‑148 (MQ=255)

                                                                                                          ctttcttATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAg                                          <  2:1629587/148‑1 (MQ=255)

                                                                                                           tttcttATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGt                                         >  2:3155823/1‑148 (MQ=255)

                                                                                                            ttcttATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGtt                                        >  1:931909/1‑148 (MQ=255)

                                                                                                             tcttATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTc                                       <  1:853492/148‑1 (MQ=255)

                                                                                                              cttATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCt                                      <  2:1252681/148‑1 (MQ=255)

                                                                                                                tATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCttt                                    <  2:3036452/148‑1 (MQ=255)

                                                                                                                tATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCttt                                    <  1:3010153/148‑1 (MQ=255)

                                                                                                                 aTCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTta                                   <  1:2312841/148‑1 (MQ=255)

                                                                                                                   cAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTtata                                 >  1:2035656/1‑148 (MQ=255)

                                                                                                                      cTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCGGCTCCTTGCCCGAAAATTTATCCTCGAGTTCTTTATaaaa                              >  1:3551430/1‑148 (MQ=255)

                                                                                                                      cTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCCGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATaaaa                              >  1:3344561/1‑148 (MQ=255)

                                                                                                                        tGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACa                            >  2:3310692/1‑148 (MQ=255)

                                                                                                                         gTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACaa                           <  1:1641560/148‑1 (MQ=255)

                                                                                                                          tAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAAt                          >  1:3805459/1‑148 (MQ=255)

                                                                                                                            aGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAATGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTc                        <  2:480095/148‑1 (MQ=255)

                                                                                                                            aGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTc                        <  1:2004291/148‑1 (MQ=255)

                                                                                                                            aGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTc                        <  1:2794333/148‑1 (MQ=255)

                                                                                                                             gacggacgTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCa                       >  2:1421334/1‑148 (MQ=255)

                                                                                                                             gacggacgTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCa                       <  2:1092124/148‑1 (MQ=255)

                                                                                                                             gacggacgTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCa                       >  1:464248/1‑148 (MQ=255)

                                                                                                                                ggacgTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTTTCTACAGTTCTCTGAGAAGCTTTTTAATTGTGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCACTc                    <  1:2833657/148‑1 (MQ=255)

                                                                                                                                ggacgTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCACTc                    <  1:918454/148‑1 (MQ=255)

                                                                                                                                  acgTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCACTCAg                  >  1:1323366/1‑148 (MQ=255)

                                                                                                                                  acgTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCACTCAg                  <  2:1499445/148‑1 (MQ=255)

                                                                                                                                  acgTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCACTCAg                  <  2:2981815/148‑1 (MQ=255)

                                                                                                                                  acgTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTAACTCAg                  >  2:3015749/1‑148 (MQ=255)

                                                                                                                                   cgTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCACTCAgg                 <  2:3142610/148‑1 (MQ=255)

                                                                                                                                     tGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCACTCAGGGt               <  2:2079832/148‑1 (MQ=255)

                                                                                                                                        aaaaTTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCACTCAGGGTTTg            >  2:902018/1‑148 (MQ=255)

                                                                                                                                          aaTTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCACTCAGGGTTTGgt          <  2:3192612/148‑1 (MQ=255)

                                                                                                                                           aTTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCACTCAGGGTTTGgtg         <  1:141193/148‑1 (MQ=255)

                                                                                                                                           aTTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCACTCAGGGTTTGgtg         <  1:3189054/148‑1 (MQ=255)

                                                                                                                                             ttAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCACTCAGGGTTTGGTGtt       >  1:3368775/1‑148 (MQ=255)

                                                                                                                                             ttAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCACTCAGGGTTTGGTGtt       <  1:2059409/148‑1 (MQ=255)

                                                                                                                                               aaTGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCACTCAGGGTTTGGTGTTCa     <  1:2950309/148‑1 (MQ=255)

                                                                                                                                                aTGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCACTCAGGGTTTGGTGTTCAt    <  2:1723455/148‑1 (MQ=255)

                                                                                                                                                 tGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCACTCAGGGTTTGGTGTTCAtt   >  2:3597125/1‑148 (MQ=255)

                                                                                                                                                 tGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCACTCAGGGTTTGGTGTTCAtt   >  2:1169166/1‑148 (MQ=255)

                                                                                                                                                 tGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTCCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCACTCAGGGTTTGGTGTTCAtt   >  2:3349271/1‑148 (MQ=255)

AGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGTGTTTTCGTTTTTATTAATTATCGTAATTTCTTTCTTATCAGCTTGTAAGACGGACGTGGAAAATTTAATGACAAAATCCTTGTTGCTAAAGATATGACGACCATTTTGTCTACAGTTCTCTGAGAAGCTTTTTAATAGAGGCGTCGCCAGCTCCTTGCCAGAAAATTTATCCTCGAGTTCTTTATAAAACAATTCACTCAGGGTTTGGTGTTCATTT  >  CP009273/4212063‑4212356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`