Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,224,513 G→T 5.6% A276A (GCG→GCT)  pgi → glucosephosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,224,5130GT 5.6% 811.5 / 14.9 224A276A (GCG→GCT) pgiglucosephosphate isomerase
Reads supporting (aligned to +/- strand):  ref base G (95/115);  new base T (6/7);  total (102/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 224 total aligned reads displayed.

AAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTA  >  CP009273/4224367‑4224656
                                                                                                                                                  |                                                                                                                                               
aaaGCGGCAGGTGATGAAAAACACTGTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGa                                                                                                                                                <  1:758613/148‑1 (MQ=255)
aaaGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGa                                                                                                                                                >  2:1088947/1‑148 (MQ=255)
  cgCGGCAGGTGATGAAACACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGAtt                                                                                                                                              <  2:3260916/147‑1 (MQ=255)
  aGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGAtt                                                                                                                                              >  2:3550944/1‑148 (MQ=255)
  aGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGAtt                                                                                                                                              <  2:3109328/148‑1 (MQ=255)
     ggCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGc                                                                                                                                           <  1:3569587/148‑1 (MQ=255)
        aGGTGATGAAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTg                                                                                                                                        <  2:1742689/148‑1 (MQ=255)
        aGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTg                                                                                                                                        <  2:1353232/148‑1 (MQ=255)
         ggTGATGAAAAACACTTTGCAAAACACATTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGt                                                                                                                                       >  2:1047465/1‑148 (MQ=255)
          gTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCTATTGGCCTGTc                                                                                                                                      >  2:291135/1‑148 (MQ=255)
            gatgaAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTTCTCTTTGTGGTGAGCGCTTGGCCTGTCGa                                                                                                                                    >  1:2564042/1‑148 (MQ=255)
            gatgaAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGa                                                                                                                                    >  1:631189/1‑148 (MQ=255)
            gatgaAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGa                                                                                                                                    <  1:1417667/148‑1 (MQ=255)
             atgaAAAACACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGAt                                                                                                                                   >  1:1924429/1‑148 (MQ=255)
                aaaaaCACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGt                                                                                                                                >  2:483231/1‑148 (MQ=255)
                   aaCACTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGCCTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGCTTGTtct                                                                                                                             >  2:1482648/1‑148 (MQ=255)
                    acacTTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTtctc                                                                                                                            >  2:1564774/1‑148 (MQ=255)
                       cGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTcc                                                                                                                         >  2:3640468/1‑148 (MQ=255)
                       cGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTcc                                                                                                                         >  2:411423/1‑148 (MQ=255)
                         ttGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCAt                                                                                                                       <  2:3183990/148‑1 (MQ=255)
                          tGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATc                                                                                                                      <  2:3486832/148‑1 (MQ=255)
                              aaaCACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTTTTATCTCCAACGGCt                                                                                                                  >  2:1957761/1‑148 (MQ=255)
                              aaaCACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCt                                                                                                                  <  2:1236071/148‑1 (MQ=255)
                              aaaCACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCt                                                                                                                  <  2:1846980/148‑1 (MQ=255)
                               aaCACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTATCTACATCGGCtt                                                                                                                 >  1:555333/1‑148 (MQ=255)
                                acacTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACAGGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTATCTCCATCGGCgtt                                                                                                                >  2:2874294/1‑148 (MQ=255)
                                  acTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGa                                                                                                              <  1:1813545/148‑1 (MQ=255)
                                   cTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGAt                                                                                                             <  2:1483889/148‑1 (MQ=255)
                                   cTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCAACgg                                                                                                                    >  1:3384868/1‑141 (MQ=255)
                                   cTTTGCGGCGCTTTCCACCAATGCCAAAGCAGTTGGCGAGTTTGGTATTGATACTGCCCACATGTTAGAGTTCTGGGACTGGGTTGGCGGCCGGTAATCTTTTTGGTCAGCGGTTGGACTGTCGATTTTTTTCAACATCgggctttat                                                                                                             >  1:2401813/1‑141 (MQ=255)
                                     ttGCGGCGCTTTCCACCAATGACAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTAGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATaa                                                                                                           <  2:2078595/148‑1 (MQ=255)
                                          gcgcTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCg                                                                                                      <  2:1916229/148‑1 (MQ=255)
                                           cgcTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGt                                                                                                     <  1:1855220/148‑1 (MQ=255)
                                           cgcTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGt                                                                                                     <  1:686784/148‑1 (MQ=255)
                                              tttCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGa                                                                                                  >  2:2686418/1‑148 (MQ=255)
                                               ttCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGaa                                                                                                 <  1:3260671/148‑1 (MQ=255)
                                               ttCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGaa                                                                                                 <  1:2670973/148‑1 (MQ=255)
                                                  caccaATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTg                                                                                              <  1:1339939/148‑1 (MQ=255)
                                                   accaATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGc                                                                                             <  1:46842/148‑1 (MQ=255)
                                                   accaATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGc                                                                                             <  2:2353296/148‑1 (MQ=255)
                                                   accaATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGc                                                                                             >  2:154629/1‑148 (MQ=255)
                                                   accaATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCAACGGCGTTGATAAATTCGTTGAAATGc                                                                                             >  1:3731757/1‑148 (MQ=255)
                                                    ccaATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCt                                                                                            <  2:770242/148‑1 (MQ=255)
                                                    ccaATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCt                                                                                            <  1:2126236/148‑1 (MQ=255)
                                                     caATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCTATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCtt                                                                                           <  2:3737255/148‑1 (MQ=255)
                                                       aTGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTc                                                                                         <  2:859485/148‑1 (MQ=255)
                                                        tGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTGACTCTTTGTGGTCAGCGATTGGCCTGTCGATTTTTGTATCCAAAGGCTTTTATAACATAGTTGTACAGCtttac                                                                                        >  1:1085012/1‑146 (MQ=255)
                                                             aaGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCGTTGTGGTCCTCGATTGGCCTTTCGATTGTTCTATAAATCGGATTTGATAACTTCGTTGAAATGCTATACGgcgc                                                                                   >  2:307740/1‑148 (MQ=255)
                                                              aGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTTAACTGCTTTCCGGCGca                                                                                  >  2:2886894/1‑148 (MQ=255)
                                                              aGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTTAACTGCTTTACGGCGca                                                                                  >  1:3123565/1‑148 (MQ=255)
                                                                   ttGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACAcgc                                                                             <  2:264548/148‑1 (MQ=255)
                                                                   ttGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCAAAGGCTTTGATAAAATCGTTGAAAAGCTTTCAGGCGCacaccc                                                                             >  1:2062937/1‑146 (MQ=255)
                                                                    tGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACAcgcg                                                                            <  1:666680/148‑1 (MQ=255)
                                                                      gCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTGTCTCCATCGGCTTTGTTAACTTCGTTGAACTGCTTTGCGGCGCACAAGCGAt                                                                          >  2:79812/1‑148 (MQ=255)
                                                                            ttGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACaa                                                                    <  1:2961431/148‑1 (MQ=255)
                                                                              ggTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGc                                                                  <  2:2956932/148‑1 (MQ=255)
                                                                                tATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCTATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCAt                                                                <  1:3439441/148‑1 (MQ=255)
                                                                                tATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGAGTTTTCTCTCCATCGGGGTTGATAACATAGTTGAACTGCTTTACGGCGCACACGCGATTGACAAGCAt                                                                >  1:3064392/1‑148 (MQ=255)
                                                                                 aTTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTTTGGTCAGCGATTTGCCTGTCGATTGTTTTATACCAAGGCGTTTATAAAATCGTTTTAAATCATTACCGCGCACACGCCATGGACAAACaat                                                               >  1:1142234/1‑146 (MQ=255)
                                                                                 aTTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCAtt                                                               <  2:345362/148‑1 (MQ=255)
                                                                                  ttGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCTATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCAttt                                                              <  1:1446503/148‑1 (MQ=255)
                                                                                   tGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTtc                                                             <  1:2893104/148‑1 (MQ=255)
                                                                                     aTACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTtctc                                                           <  2:47291/148‑1 (MQ=255)
                                                                                       aCTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCTATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTcca                                                         <  2:2240347/148‑1 (MQ=255)
                                                                                          gCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCTATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTccacca                                                      <  1:291135/148‑1 (MQ=255)
                                                                                             aaCATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGc                                                   <  1:1047465/148‑1 (MQ=255)
                                                                                             aaCATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGc                                                   <  1:1960832/148‑1 (MQ=255)
                                                                                             aaCATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGc                                                   <  1:3781993/148‑1 (MQ=255)
                                                                                              aCATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTTGTCAGCGATTTGCCTGTAGATTGTTTTCAACAAACGCGTTGATAAAATAGTTTTACATCTTTACGGCGCCCACCCCCAGGTCAAACAATTCTGCACCAcccc                                                  >  1:3233276/1‑148 (MQ=255)
                                                                                              aCATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCAAATGGCCTGTCGATTGTTCTCACCATCGGCTTTGATAAAATCGTTGAACAGCTTTACGGCGCACACGCGATGGACAACCATTTCTCCACCACGcc                                                  >  2:1361298/1‑148 (MQ=255)
                                                                                               cATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTTTTATATCCCACGGCTTTTATAAATTCGTTTAACTGCTTTCCGGCGCACACGCGATGGACCAGCATTTATCCACCACCCCt                                                 >  2:3543226/1‑148 (MQ=255)
                                                                                                  gTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTTGCCTGTCGATTGTTATATACAACGGCTTTGATAAATTAGTTGAACAGCTTTACGGCGCACACCCGATGGACAACCAATTATACACCACCCCAGcc                                              >  1:2495434/1‑148 (MQ=255)
                                                                                                   ttCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCTATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCg                                             >  2:1176869/1‑148 (MQ=255)
                                                                                                   ttCGAGTTCTGGGACTGGGTTGGCGGCCGTTACACTTTGTGGTCAGAGATTGGCCTGTAGATTTTTCTCTACAAAGGCTTTGATAACATAGATTAACATCTTTCCGGCGCAAACCCGATGGAAAAGCAATTCTACACCAccccatccc                                             >  2:3207811/1‑143 (MQ=255)
                                                                                                    tCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTTGCCTGTCGAATTTTATCTCCAAAGGCGTTGATAACATAGTTGAAAAGCTTTCCGGCGCACACCCGATGGACAAGCAATTATCCACCACCCCAGCCga                                            >  1:580534/1‑148 (MQ=255)
                                                                                                         ttCTGGGACTGGGTTGGCGGCCGTTACTCTTTTTGGTGAGCGATTGGCCTGTCGATTTTTGTATCCATAGGCGTTGTTAAAATAGTTTTAATTCTTTACGGCGCACAACCGATTGACAATCATTTAACCAACACCCCAGCCGAGaaaa                                       >  1:3610802/1‑148 (MQ=255)
                                                                                                         ttCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGaaaa                                       <  2:2450245/148‑1 (MQ=255)
                                                                                                         ttCTGGGACTGGGTTGGCGGCCGTTACACTTTTTGGTCAGCGATTGGCCTGTCGATTTTTGTATCCATCGGCTTTGATAAATTCGTTGAACTGCTTTACGGCGCCCACGCGATGGACAAGCATTTATCAACAACGCCAGCCGAGaaaa                                       >  2:52387/1‑148 (MQ=255)
                                                                                                            tGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAAcc                                    <  1:1012758/148‑1 (MQ=255)
                                                                                                              ggACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTTGCCTGTCGATTGTTCTCTCCAACGGCGTTGATAACTTAGTTTTACAGCTTTCCGGCGCACACGCGATTGACAAGCAATTCTCCACCACGCCTGCCCAGaaaaaacag                                  >  1:959437/1‑144 (MQ=255)
                                                                                                                    ggTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTa                            <  2:2584069/148‑1 (MQ=255)
                                                                                                                     gTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTAc                           <  2:725506/148‑1 (MQ=255)
                                                                                                                         gcggcCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTActgct                       <  1:1245474/148‑1 (MQ=255)
                                                                                                                           ggcCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTgg                     <  2:2006734/148‑1 (MQ=255)
                                                                                                                              cGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGgcgc                  <  1:1066007/148‑1 (MQ=255)
                                                                                                                                ttACTCTTTGTGGTCAGCGATTGGCCTGTCGATTTTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTg                >  1:1741159/1‑148 (MQ=255)
                                                                                                                                ttACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTg                <  2:3342986/148‑1 (MQ=255)
                                                                                                                                ttACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTg                >  2:3018071/1‑148 (MQ=255)
                                                                                                                                 tACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGa               >  1:2904877/1‑148 (MQ=255)
                                                                                                                                 tACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGa               <  1:2686418/148‑1 (MQ=255)
                                                                                                                                  aCTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGAt              <  2:2762062/148‑1 (MQ=255)
                                                                                                                                    tctTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTg            >  1:2770907/1‑148 (MQ=255)
                                                                                                                                    tctTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTg            >  2:2456691/1‑148 (MQ=255)
                                                                                                                                    tctTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTg            >  2:1863639/1‑148 (MQ=255)
                                                                                                                                       ttGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCa         >  2:1231668/1‑148 (MQ=255)
                                                                                                                                        tgtgGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCAt        <  1:2822120/148‑1 (MQ=255)
                                                                                                                                           ggTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTg     <  2:2112548/148‑1 (MQ=255)
                                                                                                                                            gTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTgg    >  1:3655345/1‑148 (MQ=255)
                                                                                                                                              cAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTa  >  1:883118/1‑148 (MQ=255)
                                                                                                                                              cAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTa  <  1:417444/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
AAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTA  >  CP009273/4224367‑4224656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑