Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,224,685 G→T 25.5% A334S (GCG→TCG)  pgi → glucosephosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,224,6850GT25.5% 449.3 / 119.5 210A334S (GCG→TCG) pgiglucosephosphate isomerase
Reads supporting (aligned to +/- strand):  ref base G (67/82);  new base T (24/28);  total (97/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 216 total aligned reads displayed.

ATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTA  >  CP009273/4224538‑4224832
                                                                                                                                                   |                                                                                                                                                   
ttCGGCTTTGATAACTTCGTTGAACTTCTTTCCGGCGCACACGCGATGTACAAGAATTTCTCCACCACGCCTGCAGATAAAAACCTGCCTGTACTTCTGGCGCTTATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAg                                                                                                                                                     <  2:500220/147‑1 (MQ=255)
aTCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAg                                                                                                                                                     >  1:357230/1‑148 (MQ=255)
aTCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAg                                                                                                                                                     <  2:2421601/148‑1 (MQ=255)
aTCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAg                                                                                                                                                     <  2:2421626/148‑1 (MQ=255)
   ggCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGa                                                                                                                                                  <  2:631178/148‑1 (MQ=255)
   ggCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGa                                                                                                                                                  <  2:2564042/148‑1 (MQ=255)
    gCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAATCGAt                                                                                                                                                 <  1:1088947/148‑1 (MQ=255)
    gCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAATCGAt                                                                                                                                                 <  1:2995820/148‑1 (MQ=255)
       ttGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCt                                                                                                                                              >  1:82030/1‑148 (MQ=255)
        tGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTg                                                                                                                                             >  1:3018677/1‑148 (MQ=255)
         gATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGc                                                                                                                                            >  1:105936/1‑148 (MQ=255)
            aaCTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGACACTGAATCGATTCTGCCGt                                                                                                                                         >  1:1596276/1‑148 (MQ=255)
             aCTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTa                                                                                                                                        <  2:2640663/148‑1 (MQ=255)
              cTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTAt                                                                                                                                       >  2:2474488/1‑148 (MQ=255)
              cTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTAt                                                                                                                                       >  2:1875590/1‑148 (MQ=255)
                tCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGa                                                                                                                                     >  1:2002309/1‑148 (MQ=255)
                   ttGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCa                                                                                                                                  >  1:3417056/1‑148 (MQ=255)
                    tGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAATCGATTCTGCCGTATGACCAg                                                                                                                                 >  2:915573/1‑148 (MQ=255)
                    tGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAg                                                                                                                                 >  1:3282749/1‑148 (MQ=255)
                           cTTTCCGGCGCACCCGCGATGGACAAGAATTTCACCACCCCGCCTGCCGAGAAAAACCTGCCTGTACGGCTGGCGCTGATTGGCATCTGGTACAAAAATTTCTTTGGGGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGc                                                                                                                          <  1:1459835/148‑1 (MQ=255)
                           cTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGc                                                                                                                          >  1:782190/1‑148 (MQ=255)
                           cTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTACTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGc                                                                                                                          <  2:1058967/148‑1 (MQ=255)
                                cGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGt                                                                                                                     <  1:587942/148‑1 (MQ=255)
                                 ggCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAATCGATTCTGCCGTATGACCAGTATATGCACCGtt                                                                                                                    <  1:3550944/148‑1 (MQ=255)
                                       cacGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAATCGATTCTGCCGTATGACCAGTATATGCACCGTTTCgcgg                                                                                                              <  2:935669/148‑1 (MQ=255)
                                         cgcgATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAATCGATTCTGCCGTATGACCAGTATATGCACCGTGTCgcggcg                                                                                                            >  1:2399915/1‑148 (MQ=255)
                                          gcgATGGACAAGCATGTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATGGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGt                                                                                                           <  2:2945698/148‑1 (MQ=255)
                                               gtaCAAGAATTTCACCACCACGCCGGCCGACAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTGGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTc                                                                                                      <  1:9815/146‑1 (MQ=255)
                                               ggACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTc                                                                                                      <  2:3422274/148‑1 (MQ=255)
                                               ggACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTc                                                                                                      >  2:814264/1‑148 (MQ=255)
                                                gACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAATCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTcc                                                                                                     <  2:3166577/148‑1 (MQ=255)
                                                gACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTcc                                                                                                     >  1:856091/1‑148 (MQ=255)
                                                gACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAAGTTCTTTGGTGCGGAAACTGAATCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTcc                                                                                                     <  2:1263134/148‑1 (MQ=255)
                                                  cAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAATCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCcag                                                                                                   <  1:154629/148‑1 (MQ=255)
                                                     gCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCcagcag                                                                                                >  2:2351583/1‑148 (MQ=255)
                                                       aTTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAggg                                                                                              <  2:1952880/148‑1 (MQ=255)
                                                        tttCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGc                                                                                             >  1:776569/1‑148 (MQ=255)
                                                        tttCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGc                                                                                             >  1:2694119/1‑148 (MQ=255)
                                                         ttCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAATCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCa                                                                                            <  2:2402310/148‑1 (MQ=255)
                                                         ttCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCa                                                                                            >  2:1139251/1‑148 (MQ=255)
                                                         ttCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCa                                                                                            >  1:3137171/1‑148 (MQ=255)
                                                         ttCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCa                                                                                            >  2:1824094/1‑148 (MQ=255)
                                                         ttCTCCACATCGGCTGCAGAGATAATCCTGCCTGTACTGCTGGCTCTTATTGGCATCTGGTAATACAATTTCTTTGGTGCTGATACTGACTCTATTCTGCCGTATGACCAGTTTATGGACCGTTTCGGGGCGTACTTCCTGCAGGGCa                                                                                            <  2:3763717/148‑1 (MQ=255)
                                                              ccccacCCCTGCCGAGAAAAACCTGCGTGTACTGGTGGCGCTGATGGGCATGTGGTACAACAATTTCTTGGTTGCGGAAACTGAAGAGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATg                                                                                       <  1:1482663/146‑1 (MQ=255)
                                                               accacGCCTGCCTATAAAAACCTGCGTGTACTGCTGGCGCTGATTGTCTTGTGGTAAAACAATTTATTTGGGGGGTAAACTGACGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATgg                                                                                      <  1:3207811/148‑1 (MQ=255)
                                                               accacGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATgg                                                                                      >  2:3275455/1‑148 (MQ=255)
                                                                  acGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGt                                                                                   <  2:2062937/148‑1 (MQ=255)
                                                                  acGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGt                                                                                   >  2:751427/1‑148 (MQ=255)
                                                                   cGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTc                                                                                  >  1:3009205/1‑148 (MQ=255)
                                                                    gCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTcc                                                                                 <  1:2474488/148‑1 (MQ=255)
                                                                     ccTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCa                                                                                <  2:1208889/148‑1 (MQ=255)
                                                                     ccTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCa                                                                                <  1:1564774/148‑1 (MQ=255)
                                                                     ccTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTGGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCa                                                                                <  1:745570/148‑1 (MQ=255)
                                                                         ccGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACgg                                                                            <  2:3610802/148‑1 (MQ=255)
                                                                           gagaAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAATCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTa                                                                          >  1:1363660/1‑148 (MQ=255)
                                                                            agaAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTaa                                                                         <  1:3454965/148‑1 (MQ=255)
                                                                             gaAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAg                                                                        >  2:2315577/1‑148 (MQ=255)
                                                                              aaaaaCCTGCCTGTACTGCTGGCGCTGATTGGCCACTGGGACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGGTTCGCGGCGTACTTCCAGCAGCGCCAGATGGAGTCCAACGGTAAGt                                                                       >  1:2867697/1‑148 (MQ=255)
                                                                               aaaaCCTGCCTGTCCTGCTGGCGCTGATGGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTa                                                                      <  1:3543226/148‑1 (MQ=255)
                                                                               aaaaCCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAATCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTa                                                                      <  1:1289221/148‑1 (MQ=255)
                                                                                  aCCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGt                                                                   >  2:1301770/1‑148 (MQ=255)
                                                                                           tACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAATCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCgtaa                                                          <  2:286084/148‑1 (MQ=255)
                                                                                           tACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCgtaa                                                          <  2:3123565/148‑1 (MQ=255)
                                                                                              tgctgGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCgtaacgg                                                       >  2:2761413/1‑148 (MQ=255)
                                                                                              tgctgGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCgtaacgg                                                       >  1:134360/1‑148 (MQ=255)
                                                                                              tgctgGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCgtaacgg                                                       >  1:2063785/1‑148 (MQ=255)
                                                                                              tgctgGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCgtaacgg                                                       >  2:655124/1‑148 (MQ=255)
                                                                                               gctgGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAATCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCgtaacggt                                                      <  2:950026/148‑1 (MQ=255)
                                                                                               gctgGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCgtaacggt                                                      <  1:1529041/148‑1 (MQ=255)
                                                                                                ctgGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCgtaacggta                                                     <  1:1824094/148‑1 (MQ=255)
                                                                                                ctgGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCgtaacggta                                                     <  1:1139251/148‑1 (MQ=255)
                                                                                                ctgGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCgtaacggta                                                     >  2:2830476/1‑148 (MQ=255)
                                                                                                ctgGCGCTGATTGGAATCTTGTACAACATTTTCTTGGGTGGGGAAACTTAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCgtaacggta                                                     <  1:1957761/148‑1 (MQ=255)
                                                                                                      cTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAATCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTtg                                               <  2:3443908/148‑1 (MQ=255)
                                                                                                      cTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAATCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTtg                                               <  2:3443466/148‑1 (MQ=255)
                                                                                                       tGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTtgt                                              <  1:1863639/148‑1 (MQ=255)
                                                                                                        gATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAATCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTtgtg                                             <  1:1856094/148‑1 (MQ=255)
                                                                                                         aTTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTgg                                            <  2:578164/148‑1 (MQ=255)
                                                                                                           tGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAATCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGAt                                          >  2:259046/1‑148 (MQ=255)
                                                                                                             gCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTa                                        <  2:782190/148‑1 (MQ=255)
                                                                                                              cATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATTACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTAc                                       >  2:1197902/1‑148 (MQ=255)
                                                                                                                  tGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGa                                   <  2:1085012/148‑1 (MQ=255)
                                                                                                                    gTACAACAATTTCTTTGGTGCGGAAACTGAAACGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACt                                 >  1:2643715/1‑148 (MQ=255)
                                                                                                                    gTACAACAATTTCTTTGGTGCGGAAACTGAAACGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACt                                 >  1:2268116/1‑148 (MQ=255)
                                                                                                                      acaacaATTTCTTTGGTGCGGAAACTGAAACGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTgg                               >  2:1534018/1‑148 (MQ=255)
                                                                                                                       caacaaTTTCTTTGGTGCGGAAACTGAATCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGc                              <  2:639756/148‑1 (MQ=255)
                                                                                                                         acaaTTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGccc                            <  1:518410/148‑1 (MQ=255)
                                                                                                                          caaTTTCTTTGGTGCGGAAACTGAATCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCg                           >  2:2174394/1‑148 (MQ=255)
                                                                                                                           attttATTTGTTGCGGAAAATTTAGCGATTCTGCCGTATGAACATTATATTCAACGTTTCGCGGCTTACTTACAGCTGGTCTATATTGTGTGCAACGGTAATTATGTTGACCTTAACGGTAACGTTGTGGATTACCTGACTGGCCCgt                          <  1:2844694/146‑2 (MQ=255)
                                                                                                                            aTTTCTTTGGTGCGGAAACTGAATCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGAt                         >  2:1991320/1‑148 (MQ=255)
                                                                                                                             tttCTTTGGTGCGGAAACTGAATCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGAtt                        <  1:1170107/148‑1 (MQ=255)
                                                                                                                               tCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTAt                      <  2:2499938/148‑1 (MQ=255)
                                                                                                                                 tttGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCt                    >  1:3186197/1‑148 (MQ=255)
                                                                                                                                        cGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGTGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGa             <  2:3417056/148‑1 (MQ=255)
                                                                                                                                          gAAACTGAATCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAAc           >  1:341160/1‑148 (MQ=255)
                                                                                                                                           aaaCTGCAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAAcc          <  1:2455720/148‑1 (MQ=255)
                                                                                                                                           aaaCTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAAcc          <  1:2877363/148‑1 (MQ=255)
                                                                                                                                           aaaCTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAAcc          <  2:1741159/148‑1 (MQ=255)
                                                                                                                                               tGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGc      <  1:2315577/148‑1 (MQ=255)
                                                                                                                                                 aaGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCAc    >  2:2122141/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTA  >  CP009273/4224538‑4224832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑