Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 32,802 C→A 100% I662I (ATC→ATA)  carB → carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927332,8020CA98.4% 471.3 / ‑5.0 168I662I (ATC→ATA) carBcarbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base C (0/7);  new base A (80/81);  total (80/88)
Fisher's exact test for biased strand distribution p-value = 1.44e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 169 total aligned reads displayed.

CGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATCGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACC  >  CP009273/32659‑32947
                                                                                                                                               |                                                                                                                                                 
agCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCa                                                                                                                                               <  2:1722575/147‑1 (MQ=255)
 gCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACACCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCAc                                                                                                                                              <  1:1780291/148‑1 (MQ=255)
  ccTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCAcc                                                                                                                                             >  2:1516237/1‑148 (MQ=255)
   ctctACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCa                                                                                                                                            >  2:47450/1‑148 (MQ=255)
       aCTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGccc                                                                                                                                        >  1:1206163/1‑148 (MQ=255)
             aGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGc                                                                                                                                  >  2:2852216/1‑148 (MQ=255)
             aGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGc                                                                                                                                  >  2:657411/1‑148 (MQ=255)
               ccGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTa                                                                                                                                <  2:2880257/148‑1 (MQ=255)
                     aCTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGAcc                                                                                                                          >  1:478853/1‑148 (MQ=255)
                     aCTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGAcc                                                                                                                          >  1:2399922/1‑148 (MQ=255)
                      ctctGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCg                                                                                                                         >  1:834940/1‑148 (MQ=255)
                        ctGGAATATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTg                                                                                                                       >  1:286531/1‑148 (MQ=255)
                           gAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCag                                                                                                                    >  1:2978843/1‑148 (MQ=255)
                             aGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCagaa                                                                                                                  >  1:3550423/1‑148 (MQ=255)
                             aGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCagaa                                                                                                                  >  1:1593161/1‑148 (MQ=255)
                             aGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCagaa                                                                                                                  >  1:1593167/1‑148 (MQ=255)
                               aTGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCagaaga                                                                                                                >  1:1867669/1‑148 (MQ=255)
                               aTGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCagaaga                                                                                                                >  1:2597969/1‑148 (MQ=255)
                                 gtgCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGAcc                                                                                                              <  2:1239304/148‑1 (MQ=255)
                                   gCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCCGCCCGGATGCTATCGACCGTGCAGAAGACCGt                                                                                                            <  2:1303519/148‑1 (MQ=255)
                                    cTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTCCGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTg                                                                                                           <  1:1428061/148‑1 (MQ=255)
                                    cTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTg                                                                                                           <  1:1428088/148‑1 (MQ=255)
                                      ggAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGaa                                                                                                         >  1:2404189/1‑148 (MQ=255)
                                       gAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAAc                                                                                                        >  1:164239/1‑148 (MQ=255)
                                        aaaTCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACg                                                                                                       >  1:522971/1‑148 (MQ=255)
                                         aaTCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGc                                                                                                      <  2:3483758/148‑1 (MQ=255)
                                         aaTCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGc                                                                                                      <  2:3483670/148‑1 (MQ=255)
                                         aaTCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGc                                                                                                      <  2:3483442/148‑1 (MQ=255)
                                         aaTCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGc                                                                                                      >  1:2766826/1‑148 (MQ=255)
                                          aTCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCt                                                                                                     <  2:1993858/148‑1 (MQ=255)
                                           tCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCGGGACGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCtt                                                                                                    <  1:2026808/148‑1 (MQ=255)
                                            cGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTc                                                                                                   >  1:1369695/1‑148 (MQ=255)
                                                 gTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCa                                                                                              <  2:1206163/148‑1 (MQ=255)
                                                   aTCGAGACGCCTAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACGGGCGCGCGCGCGGGAAGCTGCGGGCGTACCGGTTATCGGCACCCGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATg                                                                                            <  1:1636061/148‑1 (MQ=255)
                                                   aTCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGTCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATg                                                                                            >  1:514292/1‑148 (MQ=255)
                                                    tCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGc                                                                                           >  2:2720434/1‑148 (MQ=255)
                                                       agaAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGt                                                                                        <  2:1867669/148‑1 (MQ=255)
                                                       agaAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGt                                                                                        <  1:732390/148‑1 (MQ=255)
                                                        gaAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGtt                                                                                       >  1:1945680/1‑148 (MQ=255)
                                                        gaAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGtt                                                                                       >  2:2918394/1‑148 (MQ=255)
                                                          aGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGa                                                                                     <  2:3251368/148‑1 (MQ=255)
                                                           gCCGAATGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGACGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCCATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAg                                                                                    <  1:57422/148‑1 (MQ=255)
                                                           gCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAg                                                                                    >  1:3449621/1‑148 (MQ=255)
                                                           gCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAg                                                                                    <  1:1989522/148‑1 (MQ=255)
                                                            ccGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGc                                                                                   >  1:2030496/1‑148 (MQ=255)
                                                              gAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGt                                                                                 <  1:1070851/148‑1 (MQ=255)
                                                               aaaGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTc                                                                                >  2:1127725/1‑148 (MQ=255)
                                                               aaaGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTc                                                                                <  2:834940/148‑1 (MQ=255)
                                                               aaaGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTc                                                                                >  1:29197/1‑148 (MQ=255)
                                                               aaaGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTc                                                                                <  1:47450/148‑1 (MQ=255)
                                                               aaaGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTc                                                                                <  1:1670503/148‑1 (MQ=255)
                                                                   gCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaa                                                                            <  1:873833/148‑1 (MQ=255)
                                                                   gCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaa                                                                            <  2:1871771/148‑1 (MQ=255)
                                                                   gCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaa                                                                            <  2:3761597/148‑1 (MQ=255)
                                                                   gCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaa                                                                            >  1:2188193/1‑148 (MQ=255)
                                                                   gCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaa                                                                            <  1:3766355/148‑1 (MQ=255)
                                                                      ttATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaact                                                                         >  2:3149328/1‑148 (MQ=255)
                                                                       tATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaactg                                                                        >  2:306479/1‑148 (MQ=255)
                                                                       tATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaactg                                                                        >  2:2234703/1‑148 (MQ=255)
                                                                       tATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCACAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaactg                                                                        <  2:2604114/148‑1 (MQ=255)
                                                                        aTCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaactga                                                                       >  2:3563786/1‑148 (MQ=255)
                                                                        aTCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaactga                                                                       >  2:3564435/1‑148 (MQ=255)
                                                                          cGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTctgaaactgaaa                                                                     <  2:2013535/148‑1 (MQ=255)
                                                                            tCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAaaca                                                                   <  2:1428095/148‑1 (MQ=255)
                                                                            tCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAaaca                                                                   >  1:715957/1‑148 (MQ=255)
                                                                              cAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAaacaac                                                                 <  2:1545384/148‑1 (MQ=255)
                                                                              cAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAaacaac                                                                 <  2:3443831/148‑1 (MQ=255)
                                                                              cAGTACGGCGGTCAGACCCCGCTGAAACGGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAaacaac                                                                 <  1:3555443/148‑1 (MQ=255)
                                                                                 tACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCgg                                                              >  1:66862/1‑148 (MQ=255)
                                                                                 tACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCgg                                                              >  1:316324/1‑148 (MQ=255)
                                                                                    ggcggTCAGACCCCGCGGAAACTGGCGCGCGCGCGGGACGCTGCGGGCGTCCCGGTTATCGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGa                                                           <  1:782974/148‑1 (MQ=255)
                                                                                         tCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGcc                                                      >  2:2724628/1‑148 (MQ=255)
                                                                                          cAGACCCCGGTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCTGCCCGTATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCa                                                     <  2:3348454/148‑1 (MQ=255)
                                                                                          cAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCa                                                     >  2:1819554/1‑148 (MQ=255)
                                                                                          cAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCa                                                     >  1:2727310/1‑148 (MQ=255)
                                                                                                ccGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTa                                               <  2:164239/148‑1 (MQ=255)
                                                                                                 cGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTAc                                              >  2:1362854/1‑148 (MQ=255)
                                                                                                       aaCGGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTAt                                        <  2:1945680/148‑1 (MQ=255)
                                                                                                          tGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGa                                     >  1:1510627/1‑148 (MQ=255)
                                                                                                            gcgcgcgcgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGaaa                                   >  2:501338/1‑148 (MQ=255)
                                                                                                                   cgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGt                            <  2:1091134/148‑1 (MQ=255)
                                                                                                                   cgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGt                            >  2:2545259/1‑148 (MQ=255)
                                                                                                                   cgcTGGAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGt                            >  2:3722616/1‑148 (MQ=255)
                                                                                                                        gAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAgaga                       <  2:3120530/148‑1 (MQ=255)
                                                                                                                        gAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAgaga                       <  2:133390/148‑1 (MQ=255)
                                                                                                                        gAAGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAgaga                       <  2:1704380/148‑1 (MQ=255)
                                                                                                                         aaGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGaa                      <  2:2776123/148‑1 (MQ=255)
                                                                                                                          aGCTGCTGGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAg                     <  2:3206721/148‑1 (MQ=255)
                                                                                                                              gctgGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGa                 >  1:2282284/1‑148 (MQ=255)
                                                                                                                               ctgGCGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGaa                <  2:431797/148‑1 (MQ=255)
                                                                                                                                   cGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAagag            >  2:1704949/1‑148 (MQ=255)
                                                                                                                                   cGTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTGGAGCGTCTGAAACTGAAACACCCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAagag            <  2:3576625/148‑1 (MQ=255)
                                                                                                                                    ggaCCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAgaga           <  2:2296623/146‑1 (MQ=255)
                                                                                                                                    gTACCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAgaga           <  2:2297291/148‑1 (MQ=255)
                                                                                                                                    gTACCGGTACTCGGCACCAGCCCGCATGCTATCGACCGTGCAGAAGACCGTGAACGATTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAgaga           <  1:3078034/148‑1 (MQ=255)
                                                                                                                                      aCCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGAtt         <  1:3456918/148‑1 (MQ=255)
                                                                                                                                      aCCGGTTATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGAtt         <  1:3457609/148‑1 (MQ=255)
                                                                                                                                           ttATAGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTa    <  1:2852414/148‑1 (MQ=255)
                                                                                                                                             aTCGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACGGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTAcc  <  1:2713633/148‑1 (MQ=255)
                                                                                                                                             aTCGGCACCAGCCCGGATGATATCGACCGTTCAGAAGACCGTGAACTCTTCCAGCATGGGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTAcc  <  1:2713640/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
CGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCCGCTGAAACTGGCGCGCGCGCTGGAAGCTGCTGGCGTACCGGTTATCGGCACCAGCCCGGATGCTATCGACCGTGCAGAAGACCGTGAACGCTTCCAGCATGCGGTTGAGCGTCTGAAACTGAAACAACCGGCGAACGCCACCGTTACCGCTATTGAAATGGCGGTAGAGAAGGCGAAAGAGATTGGCTACC  >  CP009273/32659‑32947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑