Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,255,009 A→T 100% V256V (GTA→GTT)  dnaB → replicative DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,255,0090AT96.5% 736.2 / 11.7 228V256V (GTA→GTT) dnaBreplicative DNA helicase
Reads supporting (aligned to +/- strand):  ref base A (2/6);  new base T (121/99);  total (123/105)
Fisher's exact test for biased strand distribution p-value = 1.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 228 total aligned reads displayed.

ACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTACTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTATTTTG  >  CP009273/4254864‑4255153
                                                                                                                                                 |                                                                                                                                                
   ccGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAt                                                                                                                                             <  1:750139/148‑1 (MQ=255)
   ccGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAt                                                                                                                                             <  2:2207614/148‑1 (MQ=255)
   ccGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAt                                                                                                                                             <  2:605798/148‑1 (MQ=255)
      gTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCTATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtctt                                                                                                                                          <  2:3662798/148‑1 (MQ=255)
       ttATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtcttc                                                                                                                                         <  1:54954/148‑1 (MQ=255)
       ttATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGAGTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtcttc                                                                                                                                         >  1:1708509/1‑148 (MQ=255)
        tATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtcttct                                                                                                                                        <  2:72002/148‑1 (MQ=255)
        tATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtcttct                                                                                                                                        <  2:1899258/148‑1 (MQ=255)
        tATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtcttct                                                                                                                                        >  1:1227794/1‑148 (MQ=255)
        tATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtcttct                                                                                                                                        >  1:3756876/1‑148 (MQ=255)
        tATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTAtcttct                                                                                                                                        >  1:229485/1‑148 (MQ=255)
           gACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGc                                                                                                                                     >  1:93526/1‑148 (MQ=255)
             cGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTg                                                                                                                                   <  2:2441469/148‑1 (MQ=255)
               aTCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATGTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGga                                                                                                                                 <  2:3489687/148‑1 (MQ=255)
                tctcAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGgag                                                                                                                                >  1:2514842/1‑148 (MQ=255)
                tctcAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGgag                                                                                                                                >  1:3603740/1‑148 (MQ=255)
                 ctcAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCTATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGgaga                                                                                                                               >  1:133646/1‑148 (MQ=255)
                 ctcAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGgaga                                                                                                                               >  2:78265/1‑148 (MQ=255)
                       aaaaaaaCCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCAt                                                                                                                         <  1:3470700/148‑1 (MQ=255)
                         aaaaaCCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCa                                                                                                                       >  1:1586014/1‑148 (MQ=255)
                          aaaaCCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAg                                                                                                                      >  2:1116444/1‑148 (MQ=255)
                           aaaCCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGa                                                                                                                     <  2:161021/148‑1 (MQ=255)
                            aaCCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGaa                                                                                                                    >  1:1204277/1‑148 (MQ=255)
                            aaCCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGaa                                                                                                                    >  1:1205140/1‑148 (MQ=255)
                            aaCCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGaa                                                                                                                    <  1:3282783/148‑1 (MQ=255)
                              ccGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACa                                                                                                                  >  2:640023/1‑148 (MQ=255)
                               cGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAg                                                                                                                 <  1:2623667/148‑1 (MQ=255)
                                 cTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGAt                                                                                                               <  2:486863/148‑1 (MQ=255)
                                  tGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATc                                                                                                              <  1:2993596/148‑1 (MQ=255)
                                   ggCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCa                                                                                                             <  1:1009445/148‑1 (MQ=255)
                                   ggCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCa                                                                                                             <  1:2369618/148‑1 (MQ=255)
                                   ggCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCa                                                                                                             >  1:3498552/1‑148 (MQ=255)
                                   ggCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCa                                                                                                             >  2:1532084/1‑148 (MQ=255)
                                    gCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCat                                                                                                            >  2:2664046/1‑148 (MQ=255)
                                    gCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCat                                                                                                            <  2:1794466/148‑1 (MQ=255)
                                    gCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCat                                                                                                            <  2:1716159/148‑1 (MQ=255)
                                     cTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCatg                                                                                                           <  2:2012112/148‑1 (MQ=255)
                                      ttGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCatga                                                                                                          <  1:2599322/148‑1 (MQ=255)
                                      ttGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCatga                                                                                                          <  2:2708091/148‑1 (MQ=255)
                                        gCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCatgatg                                                                                                        <  2:2643172/148‑1 (MQ=255)
                                        gCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCatgatg                                                                                                        <  1:686927/148‑1 (MQ=255)
                                           gCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGt                                                                                                     >  2:1616165/1‑148 (MQ=255)
                                             cGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTtc                                                                                                   >  2:2594430/1‑148 (MQ=255)
                                             cGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTACTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTtc                                                                                                   <  2:374055/148‑1 (MQ=255)
                                               tCGGATTTGATCATCGTGGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTACTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTtctc                                                                                                 >  2:1990483/1‑148 (MQ=255)
                                               tCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTtctc                                                                                                 >  1:1239419/1‑148 (MQ=255)
                                               tCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTtctc                                                                                                 >  2:2357751/1‑148 (MQ=255)
                                               tCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTtctc                                                                                                 >  1:1444388/1‑148 (MQ=255)
                                                   aTTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGc                                                                                             >  2:2857160/1‑148 (MQ=255)
                                                   aTTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGc                                                                                             >  2:3111618/1‑148 (MQ=255)
                                                   aTTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGc                                                                                             >  1:102098/1‑148 (MQ=255)
                                                   aTTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGc                                                                                             >  1:2080266/1‑148 (MQ=255)
                                                   aTTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGc                                                                                             >  1:67886/1‑148 (MQ=255)
                                                     ttGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGt                                                                                           >  1:823315/1‑148 (MQ=255)
                                                       gATCATCGTCGCCGCGCGTCCGTCGTTGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCg                                                                                         <  1:3487887/148‑1 (MQ=255)
                                                       gATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCg                                                                                         <  2:1345531/148‑1 (MQ=255)
                                                        atcatcGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGc                                                                                        >  1:3771218/1‑148 (MQ=255)
                                                          catcGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTg                                                                                      >  1:1381317/1‑148 (MQ=255)
                                                             cgtcgCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTcg                                                                                   >  1:1916969/1‑148 (MQ=255)
                                                                cgccgcGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTcgcgc                                                                                >  1:2458090/1‑148 (MQ=255)
                                                                  ccgcGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGt                                                                              <  2:2803305/148‑1 (MQ=255)
                                                                  ccgcGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGt                                                                              <  1:3404707/148‑1 (MQ=255)
                                                                  ccgcGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGt                                                                              <  2:3679474/148‑1 (MQ=255)
                                                                       cgtccgtcGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGAcc                                                                         <  2:3603740/148‑1 (MQ=255)
                                                                        gtccgtcGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCa                                                                        <  2:1036542/148‑1 (MQ=255)
                                                                         tccgtcGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAg                                                                       >  2:330597/1‑148 (MQ=255)
                                                                         tccgtcGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAg                                                                       >  2:2255810/1‑148 (MQ=255)
                                                                          ccgtcGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGa                                                                      >  2:2825902/1‑148 (MQ=255)
                                                                                 tGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAAt                                                               <  2:456501/148‑1 (MQ=255)
                                                                                      aaaaCAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTATCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTa                                                          >  1:2112857/1‑148 (MQ=255)
                                                                                       aaaCAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTAc                                                         >  1:2934153/1‑148 (MQ=255)
                                                                                        aacaacATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTAcc                                                        >  1:1188649/1‑148 (MQ=255)
                                                                                            acaTTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGc                                                    <  1:2363304/148‑1 (MQ=255)
                                                                                                   cGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGa                                             >  1:2802287/1‑148 (MQ=255)
                                                                                                   cGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCGTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGa                                             <  2:2397355/148‑1 (MQ=255)
                                                                                                     aTGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATg                                           <  1:2574763/148‑1 (MQ=255)
                                                                                                     aTGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATg                                           <  1:2284360/148‑1 (MQ=255)
                                                                                                     aTGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATg                                           <  2:3756876/148‑1 (MQ=255)
                                                                                                       gAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGAc                                         >  1:1560784/1‑148 (MQ=255)
                                                                                                         aCCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGa                                       >  1:1963831/1‑148 (MQ=255)
                                                                                                               tcgAAAACGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTg                                 <  2:1287372/148‑1 (MQ=255)
                                                                                                                   aaaCGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGgcg                             >  2:553809/1‑148 (MQ=255)
                                                                                                                     aCGCGGCGATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGgcgcg                           >  2:2573975/1‑148 (MQ=255)
                                                                                                                        cggcgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCAt                        >  1:917776/1‑148 (MQ=255)
                                                                                                                        cggcgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCAt                        <  1:1167273/148‑1 (MQ=255)
                                                                                                                        cggcgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCAt                        <  2:1227775/148‑1 (MQ=255)
                                                                                                                        cggcgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCAt                        <  2:1227817/148‑1 (MQ=255)
                                                                                                                        cggcgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCAt                        <  2:229485/148‑1 (MQ=255)
                                                                                                                          gcgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCAttt                      <  1:60654/148‑1 (MQ=255)
                                                                                                                          gcgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCAttt                      <  2:2993525/148‑1 (MQ=255)
                                                                                                                           cgATGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTc                     >  1:161485/1‑148 (MQ=255)
                                                                                                                             aTGTTGCAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCg                   >  2:2431623/1‑148 (MQ=255)
                                                                                                                                   cAGGATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCa             <  1:383410/148‑1 (MQ=255)
                                                                                                                                      gATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATgg          >  1:387908/1‑148 (MQ=255)
                                                                                                                                      gATAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATgg          <  1:78265/148‑1 (MQ=255)
                                                                                                                                        tAAACCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGt        <  1:1616165/148‑1 (MQ=255)
                                                                                                                                           aCCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCCCCATGGGTCtt     >  2:1800223/1‑148 (MQ=255)
                                                                                                                                           aCCGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTAtt     >  2:1800568/1‑148 (MQ=255)
                                                                                                                                             cGGTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTAtttt   >  2:3817271/1‑148 (MQ=255)
                                                                                                                                              ggTTCTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTATTTTg  >  1:2654714/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ACACCGGTTATGACGATCTCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACAACATTTGCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCGGTACTTATCTTCTCGCTGGAGATGCCATCAGAACAGATCATGATGCGTTCTCTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGACGAAGACTGGGCGCGCATTTCCGGCACCATGGGTATTTTG  >  CP009273/4254864‑4255153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑