BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,258,903	G→T	6.8%	S13Y (TCT→TAT)	yjbS ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,258,903	0	G	T	6.8%	1617.3 / 49.6	440	S13Y (TCT→TAT)	yjbS	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (205/202); new base T (11/19); total (217/223)
Fisher's exact test for biased strand distribution p-value = 1.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

Only 100 of 449 total aligned reads displayed.

AAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATGCTGTATCAT  >  CP009273/4258756‑4259050

aaaaaCTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAg                                                                                                                                                     <  2:2389743/148‑1 (MQ=255)

 aaaaCTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGa                                                                                                                                                    <  1:1794746/148‑1 (MQ=255)

 aaaaCTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGa                                                                                                                                                    >  1:551670/1‑148 (MQ=255)

 aaaaCTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGa                                                                                                                                                    >  1:41719/1‑148 (MQ=255)

  aaaCTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGAt                                                                                                                                                   >  2:3598549/1‑148 (MQ=255)

   aaCTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGGGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTCGTGCATCTAGCTCAACAGGTTAAGATg                                                                                                                                                  >  2:2013042/1‑148 (MQ=255)

    aCTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGt                                                                                                                                                 <  2:3698630/148‑1 (MQ=255)

       ttACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGt                                                                                                                                              <  1:3736379/148‑1 (MQ=255)

            tGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCTTTttgt                                                                                                                                         >  2:357227/1‑148 (MQ=255)

                    aaCTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTc                                                                                                                                 >  1:684997/1‑148 (MQ=255)

                    aaCTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTc                                                                                                                                 >  1:1902653/1‑148 (MQ=255)

                      cTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTcac                                                                                                                               <  2:2917575/148‑1 (MQ=255)

                      cTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTcac                                                                                                                               >  1:2062792/1‑148 (MQ=255)

                      cTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTcac                                                                                                                               <  2:802291/148‑1 (MQ=255)

                      cTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATATAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTcac                                                                                                                               <  1:2471888/148‑1 (MQ=255)

                        tGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACat                                                                                                                             <  2:433003/148‑1 (MQ=255)

                        tGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACat                                                                                                                             <  2:519555/148‑1 (MQ=255)

                        tGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACat                                                                                                                             <  2:2806727/148‑1 (MQ=255)

                           tACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATg                                                                                                                          >  2:550123/1‑148 (MQ=255)

                            aCTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGa                                                                                                                         >  1:3605551/1‑148 (MQ=255)

                             cttcttCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGat                                                                                                                        <  2:2953874/148‑1 (MQ=255)

                               tcttctCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGatat                                                                                                                      >  2:2595141/1‑148 (MQ=255)

                                cttctCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGatata                                                                                                                     <  1:3402911/148‑1 (MQ=255)

                                 ttctCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGatatat                                                                                                                    >  1:142936/1‑148 (MQ=255)

                                    tcCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCa                                                                                                                 <  2:1655757/148‑1 (MQ=255)

                                    tcCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCa                                                                                                                 <  1:3028999/148‑1 (MQ=255)

                                    tacAGGTCTAAAAAATCTTTTGCAATTTTTAACTGAGTTTCTATGTATAAAAAGAAATCTTTGTCATAAGAGATATCTTTATTATTTAGTGCATCTAGATAAAAAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCa                                                                                                                 >  1:1012035/3‑148 (MQ=255)

                                     ccAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCat                                                                                                                >  2:2741434/1‑148 (MQ=255)

                                     ccAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCat                                                                                                                >  1:1693659/1‑148 (MQ=255)

                                         gTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATatca                                                                                                            >  2:3337760/1‑148 (MQ=255)

                                          tCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATatcat                                                                                                           >  2:2297554/1‑148 (MQ=255)

                                            tAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATatcatca                                                                                                         >  2:822706/1‑148 (MQ=255)

                                                aaaTCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCAtctc                                                                                                     <  2:1179334/148‑1 (MQ=255)

                                                 aaTCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTcc                                                                                                    <  1:1477356/148‑1 (MQ=255)

                                                   tCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCAt                                                                                                  >  1:1149698/1‑148 (MQ=255)

                                                    cTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATc                                                                                                 >  2:2400560/1‑148 (MQ=255)

                                                           aaTTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGta                                                                                          <  2:2454586/148‑1 (MQ=255)

                                                           aaTTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGta                                                                                          >  1:388400/1‑148 (MQ=255)

                                                            aTTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGtaa                                                                                         >  1:2180520/1‑148 (MQ=255)

                                                            aTTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGtaa                                                                                         <  2:1214865/148‑1 (MQ=255)

                                                              ttttAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGtaata                                                                                       >  2:3390549/1‑148 (MQ=255)

                                                               tttAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGtaataa                                                                                      >  1:1545992/1‑148 (MQ=255)

                                                                   aCTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAAtt                                                                                  >  1:3096878/1‑148 (MQ=255)

                                                                     tgtgTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTaa                                                                                >  2:1873419/1‑148 (MQ=255)

                                                                      gtgtTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAAt                                                                               <  1:2518935/148‑1 (MQ=255)

                                                                        gtTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCa                                                                             >  2:2249296/1‑148 (MQ=255)

                                                                         tttCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCAc                                                                            >  1:1622529/1‑148 (MQ=255)

                                                                          ttCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCAcc                                                                           <  2:1111250/148‑1 (MQ=255)

                                                                          ttCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCAcc                                                                           >  2:1129834/1‑148 (MQ=255)

                                                                                 tataAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTgtag                                                                    >  1:1914241/1‑148 (MQ=255)

                                                                                 tataAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTgtag                                                                    >  2:1634793/1‑148 (MQ=255)

                                                                                  ataAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTgtagg                                                                   <  1:2598501/148‑1 (MQ=255)

                                                                                    aaaaaGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATAACCATTgtagggt                                                                 >  2:2130173/1‑148 (MQ=255)

                                                                                    aaaaaGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTgtagggt                                                                 <  2:3293670/148‑1 (MQ=255)

                                                                                     aaaaGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTgtagggta                                                                <  2:3533152/148‑1 (MQ=255)

                                                                                      aaaGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTgtagggtag                                                               >  2:400394/1‑148 (MQ=255)

                                                                                      aaaGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGATATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTgtagggtag                                                               >  2:2625861/1‑148 (MQ=255)

                                                                                         gAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAgggg                                                            <  2:1987592/148‑1 (MQ=255)

                                                                                          aaaTCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAggggg                                                           <  1:2231591/148‑1 (MQ=255)

                                                                                          aaaTCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAggggg                                                           <  2:2691646/148‑1 (MQ=255)

                                                                                            aTCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCt                                                         >  1:1758091/1‑148 (MQ=255)

                                                                                              cTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTgg                                                       >  1:1225900/1‑148 (MQ=255)

                                                                                              cTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTgg                                                       >  2:3658080/1‑148 (MQ=255)

                                                                                                 tGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCa                                                    <  1:633480/148‑1 (MQ=255)

                                                                                                  ggCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCaa                                                   <  2:3597296/148‑1 (MQ=255)

                                                                                                   gCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAAt                                                  <  2:1233127/148‑1 (MQ=255)

                                                                                                    cATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATc                                                 <  2:835930/148‑1 (MQ=255)

                                                                                                    cATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATc                                                 >  1:1669554/1‑148 (MQ=255)

                                                                                                     aTAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCa                                                >  2:599250/1‑148 (MQ=255)

                                                                                                       aaGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGa                                              >  2:937064/1‑148 (MQ=255)

                                                                                                       aaGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGa                                              >  1:2723136/1‑148 (MQ=255)

                                                                                                        agagATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGaa                                             <  2:142936/148‑1 (MQ=255)

                                                                                                          agaTATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAat                                           >  1:507627/1‑148 (MQ=255)

                                                                                                           tatatCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCTGAAatc                                          <  2:3082400/147‑1 (MQ=255)

                                                                                                             tatCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAatcat                                        <  2:1326116/148‑1 (MQ=255)

                                                                                                              atCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAatcatc                                       >  1:1064133/1‑148 (MQ=255)

                                                                                                                cTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCtt                                     >  2:746645/1‑148 (MQ=255)

                                                                                                                   tattatTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTtat                                  >  2:1591703/1‑148 (MQ=255)

                                                                                                                    attattTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTtata                                 <  1:2847530/148‑1 (MQ=255)

                                                                                                                      tattTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATaaa                               >  1:2777153/1‑148 (MQ=255)

                                                                                                                        tttAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACt                             >  1:122000/1‑148 (MQ=255)

                                                                                                                         ttAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCGTTATAAACtt                            >  2:2855715/1‑148 (MQ=255)

                                                                                                                            gTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGa                         <  1:270301/148‑1 (MQ=255)

                                                                                                                             tGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGAt                        <  1:3550950/148‑1 (MQ=255)

                                                                                                                             tGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGAt                        <  2:251720/148‑1 (MQ=255)

                                                                                                                               cATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTg                      <  1:3278510/148‑1 (MQ=255)

                                                                                                                                aTCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGt                     <  2:1781899/148‑1 (MQ=255)

                                                                                                                                  cTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATAACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAAATTCGATTGttt                   >  1:968570/1‑148 (MQ=255)

                                                                                                                                    aGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGttttt                 >  1:2887149/1‑148 (MQ=255)

                                                                                                                                     gCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTg                <  2:672152/148‑1 (MQ=255)

                                                                                                                                      cTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGt               >  1:299709/1‑148 (MQ=255)

                                                                                                                                       tCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTa              <  1:3337760/148‑1 (MQ=255)

                                                                                                                                       tCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTa              <  1:807837/148‑1 (MQ=255)

                                                                                                                                       tCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTa              <  1:2400560/148‑1 (MQ=255)

                                                                                                                                        cAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTaa             >  1:3488523/1‑148 (MQ=255)

                                                                                                                                             ggTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATGCTg        <  2:3174004/148‑1 (MQ=255)

                                                                                                                                              gTAAAGATGTCGTTTTTTTTCTATTCAGATTTGATTTATTCATATCATCATCTCCATCGCTAGTAATATTATTAATCACCATTGTAGGGTTGGGTGCTGGTCAATCAGAATTCATCTTTATAAACTTCGATTGTTTTTGTAATGCTGt       <  1:2788268/148‑1 (MQ=255)

                                                                                                                                              gTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATGCTGt       <  1:747103/148‑1 (MQ=255)

                                                                                                                                                  aGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATGCTGTATCa   >  1:2354541/1‑148 (MQ=255)

                                                                                                                                                  aGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATGCTGTATCa   <  2:1914241/148‑1 (MQ=255)

AAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATGCTGTATCAT  >  CP009273/4258756‑4259050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`